Glycogen is mobilized during the disposal of peroxides by cultured astroglial cells from rat brain.

Abstract:

:Regeneration of reduced nicotinamide adenine dinucleotide phosphate (NADPH) is essential for the activity of glutathione redox cycling during cellular peroxide detoxification. In order to test for a function of astroglial glycogen to serve as endogenous precursor for glucose-6-phosphate, the substrate for the regeneration of NADPH by the pentose phosphate pathway, the content of glycogen in astroglia-rich primary cultures derived from the brains of newborn rats was determined after application of peroxides. In the presence of hydrogen peroxide or cumene hydroperoxide in concentrations of 200 microM glycogen was mobilized with a half-life of 16 min in incubation medium containing 20 mM glucose, whereas in the absence of peroxides the glycogen content decreased more slowly with a half-life of 42 min. After 30 min of incubation with or without peroxides 30 and 73%, respectively, of the initial glycogen content was found. The degree of glycogen mobilization was reduced by lowering the initial concentration of the peroxides. These results demonstrate that in astroglial cells (i) glucosyl residues of glycogen are mobilized after application of peroxides despite the presence of exogenous glucose, and (ii) that the demand for glucose-6-phosphate as substrate for NADPH regeneration via the pentose phosphate pathway can, at least partially, be met by mobilization of glycogen.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Rahman B,Kussmaul L,Hamprecht B,Dringen R

doi

10.1016/s0304-3940(00)01369-0

keywords:

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

169-72

issue

3

eissn

0304-3940

issn

1872-7972

pii

S0304394000013690

journal_volume

290

pub_type

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