Abstract：
PURPOSE:Modalities for inhibiting neovascularization may be one avenue to the development of effective therapies for retinopathy. The effect of squalamine, an antiangiogenic amino sterol, on oxygen-induced retinopathy (OIR) was assessed in a mouse model. METHODS:OIR was induced in C57BL6 mice by a 5-day exposure to 75% oxygen from postnatal day (P)7 through P12. Squalamine (25 mg/kg, subcutaneous)treated animals received either daily doses for five days from P12 to P16 or one dose just after removal from oxygen on P12. Each set of animals was killed at P17 to P21. Retinopathy was assessed with a retinopathy scoring system evaluation of retinal wholemounts and by quantification of neovascular nuclei on retinal sections. RESULTS:Animals receiving 5 days of squalamine after a 5-day exposure to oxygen had total retinopathy scores (expressed as median score with 25th and 75th quartiles in parentheses) of 4(3, 5) versus oxygen-only-reared animals with scores of 8(7, 9; P < 0.001). Animals reared in room air and animals exposed to squalamine only had similar retinopathy scores: 1(1, 2) and 1(0, 2). Oxygen-reared animals receiving single-dose squalamine also showed improvement, with a median retinopathy score of 4(4, 6.75) versus oxygen-only-reared animals with median retinopathy score of 9(7, 10; P < 0.001). There was a decreased number of neovascular nuclei extending beyond the inner limiting membrane on retinal sections in animals treated with 5 days (P < 0.01) and 1 day (P < 0.001) of squalamine. CONCLUSIONS:Squalamine significantly improved retinopathy and may be a novel agent for effective treatment of ocular neovascularization.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Higgins RD,Sanders RJ,Yan Y,Zasloff M,Williams JIkeywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
1507-12issue
6eissn
0146-0404issn
1552-5783journal_volume
41pub_type
杂志文章abstract：PURPOSE:To quantify and compare the structural and functional changes in subjects with early age-related macular degeneration (AMD), using spectral-domain optical coherence tomography (SD-OCT) and microperimetry. METHODS:Twenty-one eyes of 21 subjects with early AMD were examined. MP-1 10-2 visual fields (VFs) and SD-...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.12-10361
更新日期：2012-11-09 00:00:00
abstract：PURPOSE:Pterygia are a common, benign, fibrovascular, and infiltrative process of the corneal-conjunctival junction of unknown pathogenesis. Matrix metalloproteinases (MMPs) are a family of proteolytic enzymes active against all components of the extracellular matrix, whose activity is specifically neutralized by tissu...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.16-19410
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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abstract：:Chloride ion (Cl-) secretion by the rabbit corneal epithelium involves a catecholamine-stimulated Cl- conductance at the apical membrane, but the characteristics of the ion channel(s) responsible are unknown. A primary cell culture system was developed that induces stratified epithelial differentiation of rat and rabb...
journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：10.1167/iovs.11-8377
更新日期：2012-02-23 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1978-11-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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更新日期：2017-12-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1985-02-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.09-3651
更新日期：2009-11-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1989-07-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：10.1167/iovs.03-1239
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：1978-05-01 00:00:00
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更新日期：2015-06-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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更新日期：2016-10-01 00:00:00
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更新日期：2013-03-28 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：10.1167/iovs.10-6588
更新日期：2011-06-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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doi：
更新日期：2001-06-01 00:00:00
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更新日期：2010-03-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
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更新日期：2007-08-01 00:00:00
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journal_title：Investigative ophthalmology & visual science
pub_type： 杂志文章
doi：10.1167/iovs.10-6782
更新日期：2011-08-22 00:00:00
