当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Abstract：

:Several electrophoretic and chromatographic systems have been investigated and compared for sequence analysis of oligodeoxyribonucleotides. Three systems were found to be useful for the separation of a series of sequential degradation products resulting from a labeled oligonucleotide: (I) 2-D electrophoresisdagger; (II) 2-D PEI-cellulose; and (III) 2-D homochromatography. System (III) proved generally most informative regardless of base composition and sequence. Furthermore, only in this system will the omission of an oligonucleotide in a series of oligonucleotides be self-evident from the two-dimensional map. The sequence of up to fifteen nucleotides can be determined solely by the characteristic mobility shifts of its sequential degradation products distributed on the two-dimensional map. With this method, ten nucleotides from the double-stranded region adjacent to the left-hand 3'-terminus and seven from the right-hand 3'-terminus of bacteriophage lambda DNA have been sequenced. Similarly, nine nucleotides from the double-stranded region adjacent to the left-hand 3'-terminus and five nucleotides from the right-hand terminus of bacteriophage phi80 DNA have also been sequenced. The advantages and disadvantages of each separation system with respect to sequence analysis are discussed.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Jay E,Bambara R,Padmanabhan R,Wu R

doi

10.1093/nar/1.3.331

keywords:

subject

Has Abstract

pub_date

1974-03-01 00:00:00

pages

331-53

issue

3

eissn

0305-1048

issn

1362-4962

journal_volume

1

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • RepD-mediated recruitment of PcrA helicase at the Staphylococcus aureus pC221 plasmid replication origin, oriD.

    abstract：:Plasmid encoded replication initiation (Rep) proteins recruit host helicases to plasmid replication origins. Previously, we showed that RepD recruits directionally the PcrA helicase to the pC221 oriD, remains associated with it, and increases its processivity during plasmid unwinding. Here we show that RepD forms a co...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkp1153

    authors： Machón C,Lynch GP,Thomson NH,Scott DJ,Thomas CD,Soultanas P

    更新日期：2010-04-01 00:00:00

  • Complete nucleotide sequence of alfalfa mosaic virus RNA 4.

    abstract：:Alfalfa mosaic virus RNA 4, the subgenomic messenger for viral coat protein, was partially digested with RNase T1 or RNase A and the sequence of a number of fragments was deduced by in vitro labeling with polynucleotide kinase and application of RNA sequencing techniques. From overlapping fragments, the complete prima...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/8.10.2213

    authors： Brederode FT,Koper-Zwarthoff EC,Bol JF

    更新日期：1980-05-24 00:00:00

  • Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors.

    abstract：:Engineered derivatives of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV) possessing a unique restriction site provide a source of viral DNA that can be linearized by digestion with a specific endonuclease. Circular or linearized DNA from two such viruses were compared in terms of thei...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/18.19.5667

    authors： Kitts PA,Ayres MD,Possee RD

    更新日期：1990-10-11 00:00:00

  • p53-dependent stimulation of redox-related genes in the lymphoid organs of gamma-irradiated--mice identification of Haeme-oxygenase 1 as a direct p53 target gene.

    abstract：:Recent data showed that p53 stimulates the expression of genes encoding not only pro- but also antioxidant enzymes. It was suggested that antioxidant genes could be induced under physiologic levels of stress while the prooxidant ones respond to higher level of stress. Results presented in this article illustrate an ad...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm824

    authors： Meiller A,Alvarez S,Drané P,Lallemand C,Blanchard B,Tovey M,May E

    更新日期：2007-01-01 00:00:00

  • The mitochondrial genome of the stramenopile alga Chrysodidymus synuroideus. Complete sequence, gene content and genome organization.

    abstract：:This is the first report of a complete mitochondrial genome sequence from a photosynthetic member of the stramenopiles, the chrysophyte alga Chrysodidymus synuroideus. The circular-mapping mitochondrial DNA (mtDNA) of 34 119 bp contains 58 densely packed genes (all without introns) and five unique open reading frames ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/28.13.2512

    authors： Chesnick JM,Goff M,Graham J,Ocampo C,Lang BF,Seif E,Burger G

    更新日期：2000-07-01 00:00:00

  • Transmission of dynamic supercoiling in linear and multi-way branched DNAs and its regulation revealed by a fluorescent G-quadruplex torsion sensor.

    abstract：:DNA supercoiling is an important regulator of gene activity. The transmission of transcription-generated supercoiling wave along a DNA helix provides a way for a gene being transcribed to communicate with and regulate its neighboring genes. Currently, the dynamic behavior of supercoiling transmission remains unclear o...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky534

    authors： Xia Y,Zheng KW,He YD,Liu HH,Wen CJ,Hao YH,Tan Z

    更新日期：2018-08-21 00:00:00

  • Crystal structure of the P2 C-repressor: a binder of non-palindromic direct DNA repeats.

    abstract：:As opposed to the vast majority of prokaryotic repressors, the immunity repressor of temperate Escherichia coli phage P2 (C) recognizes non-palindromic direct repeats of DNA rather than inverted repeats. We have determined the crystal structure of P2 C at 1.8 Å. This constitutes the first structure solved from the fam...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq626

    authors： Massad T,Skaar K,Nilsson H,Damberg P,Henriksson-Peltola P,Haggård-Ljungquist E,Högbom M,Stenmark P

    更新日期：2010-11-01 00:00:00

  • The nucleotide sequence of the nitrogen-regulation gene ntrA of Klebsiella pneumoniae and comparison with conserved features in bacterial RNA polymerase sigma factors.

    abstract：:The nucleotide sequence of the Klebsiella pneumoniae ntrA gene has been determined. NtrA encodes a 53,926 Dalton acidic polypeptide; a calculated molecular weight which is significantly lower than that determined by SDS polyacrylamide gel analysis. NtrA is followed by another open-reading frame (orf) of at least 75 am...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/13.21.7607

    authors： Merrick MJ,Gibbins JR

    更新日期：1985-11-11 00:00:00

  • A sequence motif in many polymerases.

    abstract：:A 15-residue sequence motif has been found in many polymerases from various species and involving DNA and RNA dependence and product. The motif is characterized by a Tyr-Gly-Asp-(Thr)-Asp core flanked by hydrophobic spans five residues in length. An mRNA maturase segment is also suggested to display the motif pattern....

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/16.21.9909

    authors： Argos P

    更新日期：1988-11-11 00:00:00

  • INTERSPIA: a web application for exploring the dynamics of protein-protein interactions among multiple species.

    abstract：:Proteins perform biological functions through cascading interactions with each other by forming protein complexes. As a result, interactions among proteins, called protein-protein interactions (PPIs) are not completely free from selection constraint during evolution. Therefore, the identification and analysis of PPI c...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky378

    authors： Kwon D,Lee D,Kim J,Lee J,Sim M,Kim J

    更新日期：2018-07-02 00:00:00

  • Stable complex formation of CENP-B with the CENP-A nucleosome.

    abstract：:CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetitive DNA. In the present study, we found that the CENP-A nucleosome more stably re...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkv405

    authors： Fujita R,Otake K,Arimura Y,Horikoshi N,Miya Y,Shiga T,Osakabe A,Tachiwana H,Ohzeki J,Larionov V,Masumoto H,Kurumizaka H

    更新日期：2015-05-26 00:00:00

  • Identification of microRNA clusters cooperatively acting on epithelial to mesenchymal transition in triple negative breast cancer.

    abstract：:MicroRNAs play important roles in many biological processes. Their aberrant expression can have oncogenic or tumor suppressor function directly participating to carcinogenesis, malignant transformation, invasiveness and metastasis. Indeed, miRNA profiles can distinguish not only between normal and cancerous tissue but...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkz016

    authors： Cantini L,Bertoli G,Cava C,Dubois T,Zinovyev A,Caselle M,Castiglioni I,Barillot E,Martignetti L

    更新日期：2019-03-18 00:00:00

  • ExPASy: SIB bioinformatics resource portal.

    abstract：:ExPASy (http://www.expasy.org) has worldwide reputation as one of the main bioinformatics resources for proteomics. It has now evolved, becoming an extensible and integrative portal accessing many scientific resources, databases and software tools in different areas of life sciences. Scientists can henceforth access s...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks400

    authors： Artimo P,Jonnalagedda M,Arnold K,Baratin D,Csardi G,de Castro E,Duvaud S,Flegel V,Fortier A,Gasteiger E,Grosdidier A,Hernandez C,Ioannidis V,Kuznetsov D,Liechti R,Moretti S,Mostaguir K,Redaschi N,Rossier G,Xenarios

    更新日期：2012-07-01 00:00:00

  • NMR structure of a vestigial nuclease provides insight into the evolution of functional transitions in viral dsDNA packaging motors.

    abstract：:Double-stranded DNA viruses use ATP-powered molecular motors to package their genomic DNA. To ensure efficient genome encapsidation, these motors regulate functional transitions between initiation, translocation, and termination modes. Here, we report structural and biophysical analyses of the C-terminal domain of the...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa874

    authors： Mahler BP,Bujalowski PJ,Mao H,Dill EA,Jardine PJ,Choi KH,Morais MC

    更新日期：2020-11-18 00:00:00

  • Non-canonical translation initiation of the spliced mRNA encoding the human T-cell leukemia virus type 1 basic leucine zipper protein.

    abstract：:Human T-cell leukemia virus type 1 (HTLV-1) is the etiological agent of adult T-cell leukemia (ATL). The HTLV-1 basic leucine zipper protein (HBZ) is expressed in all cases of ATL and is directly associated with virus pathogenicity. The two isoforms of the HBZ protein are synthesized from antisense messenger RNAs (mRN...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gky802

    authors： Cáceres CJ,Angulo J,Lowy F,Contreras N,Walters B,Olivares E,Allouche D,Merviel A,Pino K,Sargueil B,Thompson SR,López-Lastra M

    更新日期：2018-11-16 00:00:00

  • Parallel intramolecular DNA triple helix with G and T bases in the third strand stabilized by Zn(2+) ions.

    abstract：:We present evidence of formation of an intramolecular parallel triple helix with T*A.T and G*G.C base triplets (where * represents the hydrogen bonding interaction between the third strand and the duplex while. represents the Watson-Crick interactions which stabilize the duplex). The third GT strand, containing seven ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/28.18.3511

    authors： Khomyakova EB,Gousset H,Liquier J,Huynh-Dinh T,Gouyette C,Takahashi M,Florentiev VL,Taillandier E

    更新日期：2000-09-15 00:00:00

  • Argonaute binding within 3'-untranslated regions poorly predicts gene repression.

    abstract：:Despite two decades of study, the full scope of RNAi in mammalian cells has remained obscure. Here we combine: (i) Knockout of argonaute (AGO) variants; (ii) RNA sequencing analysis of gene expression changes and (iii) Enhanced Crosslinking Immunoprecipitation Sequencing (eCLIP-seq) using anti-AGO2 antibody to identif...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa478

    authors： Chu Y,Kilikevicius A,Liu J,Johnson KC,Yokota S,Corey DR

    更新日期：2020-07-27 00:00:00

  • Isolation and partial characterisation of a Chinese hamster O6-alkylguanine-DNA alkyltransferase cDNA.

    abstract：:The cDNA encoding Chinese hamster O6-alkylguanine-DNA-alkyltransferase (ATase) has been isolated from a library prepared from RNA isolated from V79 lung fibroblasts which had an upregulated level of this repair activity following stepwise selection with a chloroethylating agent (1, 2). Expression of the cDNA in E. col...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/20.8.1891

    authors： Rafferty JA,Elder RH,Watson AJ,Cawkwell L,Potter PM,Margison GP

    更新日期：1992-04-25 00:00:00

  • Demonstration of a word design strategy for DNA computing on surfaces.

    abstract：:A strategy for DNA computing on surfaces using linked sets of 'DNA words' that are short oligonucleotides (16mers) is proposed. The 16mer words have the format 5'-FFFFvvvvvvvvFFFF-3' in which 4-8 bits of data are stored in 8 variable ('v') base locations, and the remaining fixed ('F') base locations are used as a word...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/25.23.4748

    authors： Frutos AG,Liu Q,Thiel AJ,Sanner AM,Condon AE,Smith LM,Corn RM

    更新日期：1997-12-01 00:00:00

  • H-InvDB in 2009: extended database and data mining resources for human genes and transcripts.

    abstract：:We report the extended database and data mining resources newly released in the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). H-InvDB is a comprehensive annotation resource of human genes and transcripts, and consists of two main views and six sub-databases. The latest release of H-InvDB (release 6...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkp1020

    authors： Yamasaki C,Murakami K,Takeda J,Sato Y,Noda A,Sakate R,Habara T,Nakaoka H,Todokoro F,Matsuya A,Imanishi T,Gojobori T

    更新日期：2010-01-01 00:00:00

  • The folding of the hepatitis C virus internal ribosome entry site depends on the 3'-end of the viral genome.

    abstract：:Hepatitis C virus (HCV) translation initiation is directed by an internal ribosome entry site (IRES) and regulated by distant regions at the 3'-end of the viral genome. Through a combination of improved RNA chemical probing methods, SHAPE structural analysis and screening of RNA accessibility using antisense oligonucl...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks927

    authors： Romero-López C,Barroso-Deljesus A,García-Sacristán A,Briones C,Berzal-Herranz A

    更新日期：2012-12-01 00:00:00

  • The MH1 domain of Smad3 interacts with Pax6 and represses autoregulation of the Pax6 P1 promoter.

    abstract：:Pax6 transcription is under the control of two main promoters (P0 and P1), and these are autoregulated by Pax6. Additionally, Pax6 expression is under the control of the TGFbeta superfamily, although the precise mechanisms of such regulation are not understood. The effect of TGFbeta on Pax6 expression was studied in t...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkl1105

    authors： Grocott T,Frost V,Maillard M,Johansen T,Wheeler GN,Dawes LJ,Wormstone IM,Chantry A

    更新日期：2007-01-01 00:00:00

  • Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism.

    abstract：:The human MTH1 gene for 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase, produces seven types (types 1, 2A, 2B, 3A, 3B, 4A and 4B) of mRNAs. The B-type mRNAs with exon 2b-2c segments have three additional in-frame AUGs in their 5' regions. We report here that these transcripts produce three forms of MTH1 polypeptides (...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/27.22.4335

    authors： Oda H,Taketomi A,Maruyama R,Itoh R,Nishioka K,Yakushiji H,Suzuki T,Sekiguchi M,Nakabeppu Y

    更新日期：1999-11-15 00:00:00

  • Two self-splicing group I introns in the ribonucleotide reductase large subunit gene of Staphylococcus aureus phage Twort.

    abstract：:We have recently described three group I introns inserted into a single gene, orf142, of the staphylococcal bacteriophage Twort and suggested the presence of at least two additional self-splicing introns in this phage genome. Here we report that two previously uncharacterized introns, 429 and 1087 nt in length, interr...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/30.9.1935

    authors： Landthaler M,Begley U,Lau NC,Shub DA

    更新日期：2002-05-01 00:00:00

  • Identification, cloning and characterization of a new DNA-binding protein from the hyperthermophilic methanogen Methanopyrus kandleri.

    abstract：:Three novel DNA-binding proteins with apparent molecular masses of 7, 10 and 30 kDa have been isolated from the hyperthermophilic methanogen Methanopyrus kandleri. The proteins were identified using a blot overlay assay that was modified to emulate the high ionic strength intracellular environment of M.kandleri protei...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/30.3.685

    authors： Pavlov NA,Cherny DI,Nazimov IV,Slesarev AI,Subramaniam V

    更新日期：2002-02-01 00:00:00

  • High-resolution microbial community reconstruction by integrating short reads from multiple 16S rRNA regions.

    abstract：:The emergence of massively parallel sequencing technology has revolutionized microbial profiling, allowing the unprecedented comparison of microbial diversity across time and space in a wide range of host-associated and environmental ecosystems. Although the high-throughput nature of such methods enables the detection...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt1070

    authors： Amir A,Zeisel A,Zuk O,Elgart M,Stern S,Shamir O,Turnbaugh PJ,Soen Y,Shental N

    更新日期：2013-12-01 00:00:00

  • Selection of bacteriophage lambda integrases with altered recombination specificity by in vitro compartmentalization.

    abstract：:In vitro compartmentalization (IVC) was employed for the first time to select for novel bacteriophage lambda integrase variants displaying significantly enhanced recombination activity on a non-cognate target DNA sequence. These variants displayed up to 9-fold increased recombination activity over the parental enzyme,...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkp1089

    authors： Tay Y,Ho C,Droge P,Ghadessy FJ

    更新日期：2010-03-01 00:00:00

  • The two Saccharomyces cerevisiae SUA7 (TFIIB) transcripts differ at the 3'-end and respond differently to stress.

    abstract：:Despite much information as to the structure and function of the general transcription factors, little is known about the regulation of their expression. Transcription of the Saccharomyces cerevisiae SUA7 (TFIIB) gene results in the formation of two discrete transcripts. It was originally reported that the two transcr...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/28.22.4435

    authors： Hoopes BC,Bowers GD,DiVisconte MJ

    更新日期：2000-11-15 00:00:00

  • Electrochemical detection of mismatched DNA using a MutS probe.

    abstract：:A direct and label-free electrochemical biosensor for the detection of the protein-mismatched DNA interaction was designed using immobilized N-terminal histidine tagged Escherichia coli. MutS on a Ni-NTA coated Au electrode. General electrochemical methods, cyclic voltammetry (CV), electrochemical quartz crystal micro...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkl364

    authors： Cho M,Lee S,Han SY,Park JY,Rahman MA,Shim YB,Ban C

    更新日期：2006-06-13 00:00:00

  • Two zinc finger proteins from Xenopus laevis bind the same region of 5S RNA but with different nuclease protection patterns.

    abstract：:Immature oocytes from Xenopus laevis contain a 42S ribonucleoprotein particle (RNP) containing 5S RNA, tRNA, a 43 kDa protein, and a 48 kDa protein. A particle containing 5S RNA and the 43 kDa protein (p43-5S) liberated from the 42S particle upon brief treatment with urea can be purified by anion exchange chromatograp...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/19.8.1797

    authors： Sands MS,Bogenhagen DF

    更新日期：1991-04-25 00:00:00