Abstract:
:The possibility of sperm as a vehicle to deliver foreign DNA to oocytes was tested in hamsters. Epididymal spermatozoa, incubated with linearized plasmid DNA encoding ovine growth hormone (pCMXoGH), showed a spontaneous tendency to interact with DNA. Kinetics of sperm uptake of DNA was determined by using [32P]-labeled DNA. Spermatozoa took up the added DNA by 15-30 min and the uptake was inhibited by human seminal fluid in a dose dependent manner. Addition of DNA did not affect the functional competence of spermatozoa, in terms of their ability to undergo capacitation and acrosome reaction (34.5% +/- 2.2 vs 35% +/- 1.5). The fertilizing ability of DNA treated-spermatozoa from hamsters and humans was assessed by zona-free hamster egg penetration assay. Number of sperm penetrated per oocyte were 23 +/- 4.5 and 1.4 +/- 1.3 for hamster and human spermatozoa, respectively. Penetrated oocytes harbored sperm-treated DNA both with hamster (30.2 cpm/oocyte) and human (19.2 cpm/oocyte) spermatozoa. These results show that the hamster and human spermatozoa have a strong tendency to interact with exogenous (foreign) DNA and are able to transfer DNA to oocytes. Sperm may be used as a vector for DNA transfer and this approach has potential in the production of transgenic animals.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Fernandez MA,Mani SA,Rangarajan PN,Seshagiri PBkeywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
1085-92issue
11eissn
0019-5189issn
0975-1009journal_volume
37pub_type
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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更新日期:2005-08-01 00:00:00
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journal_title:Indian journal of experimental biology
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更新日期:2007-11-01 00:00:00
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
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更新日期:2004-11-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:2001-06-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:2001-10-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
doi:
更新日期:2003-10-01 00:00:00
abstract::Three lipophilic amide derivatives of phthaloyl-GABA (P-GABA), namely gamma-phthalimido N-amyl butyramide (PGA), gamma-phthalimido-N-hexylbutyramide (PGH) and gamma-phthalimido N-phenylbutyramide (PGP), were synthesized and evaluated for their hypnotic and anticonvulsant activities in mice. Both PGA and PGH showed mod...
journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1992-07-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
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journal_title:Indian journal of experimental biology
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doi:
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journal_title:Indian journal of experimental biology
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更新日期:1997-06-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
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更新日期:2013-07-01 00:00:00
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journal_title:Indian journal of experimental biology
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doi:
更新日期:1992-06-01 00:00:00