Abstract:
:Culture filtrate from Mycobacterium tuberculosis contains protective molecules which have been used successfully in experimental vaccines against tuberculosis. Despite an increasing number of mycobacterial proteins being characterised, a major effort is still needed to get an overview of the many potentially interesting molecules in culture filtrate. In this study we describe a high throughput method for purification and biological evaluation of protein components in complex protein mixtures. The method presents a new application of the recently developed Mini Whole Gel Eluter and employs this apparatus for the high resolution electroelution of selected molecular mass fractions of protein mixtures previously separated in large polyacrylamide gels. Two novel M. tuberculosis culture filtrate proteins (CspA and TB18.6) were purified by this method, their N-terminal sequences were determined and the open reading frame encoding each of the proteins identified. The immunological recognition of the molecules were evaluated in tuberculosis infected mice and guinea pigs. Both proteins induced DTH responses in guinea pigs and IFN-gamma release from spleen lymphocytes isolated from infected mice.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Weldingh K,Hansen A,Jacobsen S,Andersen Pdoi
10.1046/j.1365-3083.2000.00655.xkeywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
79-86issue
1eissn
0300-9475issn
1365-3083pii
sji655journal_volume
51pub_type
杂志文章abstract::Nowadays, people pay more attention to biomarkers that can predict clinical efficacy of immunotherapy for allergic rhinitis. As the only recognized aetiological treatment, the efficacy of allergen immunotherapy (AIT) has been proved by many studies. However, treatment success depends on compliance and persistence grea...
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journal_title:Scandinavian journal of immunology
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