Abstract:
:Recent findings have suggested that oxidative damage might contribute to the cytotoxicity and carcinogenicity of aflatoxin B1 (AFB1). Salvia miltiorrhiza (Sm), a herbal plant that has been used extensively in traditional Chinese medicine for treating cardiovascular and liver diseases, is believed to have some antioxidative capabilities. In this study, the protective effect of Sm against AFB1-induced cytotoxicity was investigated in cultured primary rat hepatocytes. AFB1-induced cytotoxicity and lipid peroxidation (LPO) were estimated by determination of lactate dehydrogenase (LDH) leakage and thiobarbituric acid reactive substances (TBARS) formation, respectively. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2',7'-dichlorofluorescein diacetate (DCFH-DA). In addition, changes of intracellular glutathione (GSH) content were also studied. Results showed that Sm was able to suppress the LDH leakage induced by AFB1 in a dose-dependent manner. A dose-dependent inhibitory effect of Sm on AFB1-induced LPO was also found in hepatocytes treated with Sm. It was further observed that Sm produced an inhibitory effect on ROS formation caused by AFB1. Concomitantly, the GSH content in Sm-treated groups increased substantially compared to those without Sm treatment. These findings suggest that Sm can inhibit the cytotoxicity of AFB1 through decreasing ROS formation, inhibiting LPO and preventing GSH depletion. The major component of the aqueous extract of Sm was identified by using high performance liquid chromatography (HPLC), proton magnetic resonance (1H-NMR) and mass spectrum (MS). Analytical results suggested that D(+)beta3,4-dihydroxyphenol lactic acid (DA) is the main compound of the aqueous extract of Sm.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Liu J,Yang CF,Lee BL,Shen HM,Ang SG,Ong CNdoi
10.1080/10715769900301131keywords:
subject
Has Abstractpub_date
1999-12-01 00:00:00pages
559-68issue
6eissn
1071-5762issn
1029-2470journal_volume
31pub_type
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journal_title:Free radical research
pub_type: 杂志文章,评审
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journal_title:Free radical research
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Free radical research
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pub_type: 杂志文章,随机对照试验
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更新日期:2012-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Free radical research
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
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更新日期:1999-10-01 00:00:00
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更新日期:2018-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.978311
更新日期:2015-01-01 00:00:00
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journal_title:Free radical research
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doi:10.3109/10715769709097838
更新日期:1997-07-01 00:00:00
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更新日期:1996-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
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更新日期:2001-04-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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更新日期:2014-02-01 00:00:00
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journal_title:Free radical research
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更新日期:2016-09-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00