L-glutamate suppresses amyloid beta-protein-induced stellation of cultured rat cortical astrocytes.

abstract：:We report on the expression of ionotropic glutamate receptor subunits in primary neuronal cultures from rat cortex, hippocampus and cerebellum and of metabotropic glutamate (mGlu) receptor subtypes in these neuronal cultures as well as in cortical astroglial cultures. We found that the NMDA receptor (NR) subunits NR1,...

journal_title：Journal of neurochemistry

authors： Janssens N,Lesage AS

更新日期：2001-06-01 00:00:00

abstract：:The uptake of glutamate and other acidic amino acids into barnacle single muscle fibres has been characterized. The uptake of glutamate consists of two components, one Na-independent and one Na-dependent. The Na-dependent uptake is saturable (half-maximal at 250 microM external glutamate) and is inhibited by a variety...

journal_title：Journal of neurochemistry

authors： Revest PA,Baker PF

更新日期：1988-01-01 00:00:00

abstract：:A mass fragmentographic method for the determination of trace amounts of amino acid neurotransmitter candidates from brain perfusates is described. The analytical procedure includes the measurements of glycine, beta-alanine, gamma-aminobutyric acid, proline, aspartic acid, and glutamic acid; alpha-alanine, leucine, an...

journal_title：Journal of neurochemistry

authors： Wolfensberger M,Amsler U

更新日期：1982-02-01 00:00:00

abstract：:Addition of vasoactive intestinal peptide (VIP) to brain homogenates increased the activity of choline acetyltransferase (ChAT) but not that of acetylcholinesterase or glucose-6-phosphate dehydrogenase. Activity of ChAT was increased in the anterior hypothalamus and in the dorsal and ventral hippocampus, but not in th...

journal_title：Journal of neurochemistry

authors： Luine VN,Rostene W,Rhodes J,McEwen BS

更新日期：1984-04-01 00:00:00

abstract：:In human fetal spinal cord (HFSC), six additional microtubule-associated protein-2 (MAP-2) transcripts are generated by alternative splicing of two recently described exons, exon 8 and exon 13. The following three translated proteins are detected by western blot analysis: MAP-2b expressing exon 8 (MAP-2b + 8; MAP-2a),...

journal_title：Journal of neurochemistry

authors： Kalcheva N,Weidenheim KM,Kress Y,Shafit-Zagardo B

更新日期：1997-01-01 00:00:00

abstract：:Traumatic brain injury (TBI) is a complex injury involving several physiological alterations, potentially leading to neurological impairment. Previous mouse studies using high-density oligonucleotide array analysis have confirmed the upregulation of transforming growth-interacting factor (TGIF) mRNA in TBI. TGIF is a ...

journal_title：Journal of neurochemistry

authors： Chio CC,Chang CP,Lin MT,Su FC,Yang CZ,Tseng HY,Liu ZM,Huang HS

更新日期：2014-12-01 00:00:00

abstract：:Using nuclear reaction 6Li(n, alpha)3H and dielectric detectors, we have studied the distribution of Li in the brain of adult mice, following Li treatment of the animals. Two strains of animals were used in parallel: "quaking" dysmyelinating mutants and normally myelinated controls. The distribution appeared to be sha...

journal_title：Journal of neurochemistry

authors： Heurteaux C,Baumann N,Lachapelle F,Wissocq JC,Thellier M

更新日期：1986-04-01 00:00:00

abstract：:Glycine and GABA are likely co-transmitters in the spinal cord. Their possible interactions in presynaptic terminals have, however, not been investigated. We studied the effects of glycine on GABA release using superfused mouse spinal cord synaptosomes. Glycine concentration dependently elicited [(3)H]GABA release whi...

journal_title：Journal of neurochemistry

authors： Raiteri L,Raiteri M,Bonanno G

更新日期：2001-03-01 00:00:00

abstract：:The myelin-deficient (mld) mouse is an autosomal recessive mutant characterized by hypomyelination of the CNS due to reduced expression of the myelin basic protein (MBP) gene. In the mld mutant, the MBP gene is duplicated in tandem. One gene is intact, but a large portion is inverted upstream of the other copy, and it...

journal_title：Journal of neurochemistry

authors： Okano H,Aruga J,Nakagawa T,Shiota C,Mikoshiba K

更新日期：1991-02-01 00:00:00

abstract：:Terminally differentiated PC12 cells are a useful neuron-like model for studying programmed cell death in response to nerve growth factor (NGF) deprivation. This in vitro model was used to investigate the mechanism by which cyanide-induced histotoxic hypoxia produces neuronal degeneration. Treatment of undifferentiate...

journal_title：Journal of neurochemistry

authors： Mills EM,Gunasekar PG,Pavlakovic G,Isom GE

更新日期：1996-09-01 00:00:00

abstract：:Neuron-astrocyte interactions are critical for signalling, energy metabolism, extracellular ion and glutamate homeostasis, volume regulation and neuroprotection in the CNS. Glutamate uptake by astrocytes may prevent excitotoxic glutamate elevation and determine neuronal survival. However, an excess of glutamate can ca...

journal_title：Journal of neurochemistry

authors： Szydlowska K,Zawadzka M,Kaminska B

更新日期：2006-11-01 00:00:00

abstract：:Increased arachidonic acid release occurred during activation of catecholamine secretion from cultured bovine adrenal medullary chromaffin cells. The nicotinic agonist 1,1-dimethyl-4- phenylpiperazinium (DMPP) caused an increased release of preincubated [3H]arachidonic acid over a time course which corresponded to the...

journal_title：Journal of neurochemistry

authors： Frye RA,Holz RW

更新日期：1984-07-01 00:00:00

abstract：:The pH dependency of the binding of ligands to adenosine A2a receptors in rat striatal membranes was examined. For those agonists sensitive to adenosine deaminase a solubilised membrane preparation was used. A two- to fourfold increase in affinity was observed for CGS-21680, 5'-N-ethylcarboxamidoadenosine, adenosine, ...

journal_title：Journal of neurochemistry

authors： Askalan R,Richardson PJ

更新日期：1994-10-01 00:00:00

abstract：:Three unique 5' untranslated regions (UTRs) have been characterized for human microtubule-associated protein-2 (MAP-2) transcripts. All three UTRs shared a common 171-bp sequence adjacent to the MAP-2 coding region and then diverged upstream. The size of the unique upstream sequence was 281, 146, or 104 bp. PCR of gen...

journal_title：Journal of neurochemistry

authors： Kalcheva N,Shafit-Zagardo B

更新日期：1995-10-01 00:00:00

abstract：:There is ample evidence that both acid (ASMase) and neutral (NSMase) sphingomyelinases play a role in cell death so inhibitors of either enzyme could have significant value as protectors against neurodegeneration. We used a fluorogenic sphingomyelinase substrate, 6-hexadecanoylamino-4-methylumbelliferyl-phosphorylchol...

journal_title：Journal of neurochemistry

authors： Testai FD,Landek MA,Goswami R,Ahmed M,Dawson G

更新日期：2004-05-01 00:00:00

abstract：:Perhaps the most reproducible early event induced by the interaction of amyloid beta peptide (A beta) with the cell is the inhibition of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. We recently demonstrated that cytotoxic amyloid peptides such as A beta and human amylin inhibi...

journal_title：Journal of neurochemistry

authors： Liu Y,Schubert D

更新日期：1998-12-01 00:00:00

abstract：:Thymosin beta 4 (Tβ4), a secreted 43 amino acid peptide, promotes oligodendrogenesis, and improves neurological outcome in rat models of neurologic injury. We demonstrated that exogenous Tβ4 treatment up-regulated the expression of the miR-200a in vitro in rat brain progenitor cells and in vivo in the peri-infarct are...

journal_title：Journal of neurochemistry

authors： Santra M,Chopp M,Santra S,Nallani A,Vyas S,Zhang ZG,Morris DC

更新日期：2016-01-01 00:00:00

abstract：:Knowledge of the relative contributions of mast cells and neurons to the overall pool of histamine in the brain is a prerequisite to determining the significance and role of this amine in brain function. Consequently, we analyzed the levels of brain histamine in four genotypes (+/+, W/+, Wv/+, and W/Wv) of WBB6F1 mice...

journal_title：Journal of neurochemistry

authors： Orr EL,Pace KR

更新日期：1984-03-01 00:00:00

abstract：:In this study, we examined the molecular and functional characterization of choline uptake into cultured rat cortical astrocytes. Choline uptake into astrocytes showed little dependence on extracellular Na+. Na+-independent choline uptake was saturable and mediated by a single transport system, with an apparent Michae...

journal_title：Journal of neurochemistry

authors： Inazu M,Takeda H,Matsumiya T

更新日期：2005-09-01 00:00:00

abstract：:Staufen is an RNA-binding protein, first identified for its role in oogenesis and CNS development in Drosophila. Two mammalian homologs of Staufen have been identified and shown to bind double-stranded RNA and tubulin, and to function in the somatodendritic transport of mRNA in neurons. Here, we examined whether Stauf...

journal_title：Journal of neurochemistry

authors： Bélanger G,Stocksley MA,Vandromme M,Schaeffer L,Furic L,DesGroseillers L,Jasmin BJ

更新日期：2003-08-01 00:00:00

abstract：:Five-day-old rats subjected to prolonged asphyxia at 37 degrees C show a slower rate of synthesis of 5-HT as measured by the accumulation of the amine after inhibition of monoamine oxidase. During recovery from asphyxia 5-HT synthesis is markedly stimulated when measured over a 50 or 90 min period. No change in 5-HT s...

journal_title：Journal of neurochemistry

authors： Tricklebank MD,Pickard FJ,Dobbing J

更新日期：1976-11-01 00:00:00

abstract：:Animals trained in a passive avoidance task exhibit a transient time-dependent increase in hippocampal neural cell adhesion molecule (NCAM) polysialylation at 12-24 h following the initial learning trial. Using immunocytochemical techniques with a monoclonal antibody that specifically recognises NCAM-polysialic acid h...

journal_title：Journal of neurochemistry

authors： Fox GB,O'Connell AW,Murphy KJ,Regan CM

更新日期：1995-12-01 00:00:00

abstract：:Pumiliotoxins (PTX) A, B, and 251D, members of a new class of indolizidine alkaloids isolated from the skin of poison frogs of the family Dendrobatidae, inhibit Ca2+-ATPase activity in sarcoplasmic reticulum vesicles from frog and rat hind-limb muscles. PTX-B and PTX-A appear to be relatively specific inhibitors of Ca...

journal_title：Journal of neurochemistry

authors： Tamburini R,Albuquerque EX,Daly JW,Kauffman FC

更新日期：1981-09-01 00:00:00

abstract：:The mu-opioid receptor agonists have a preferential effect on nociception in adults but their analgesic effect is less selective in neonates. Here we presented our finding that the mu-opioid receptor agonists had no effect on high voltage-activated Ca(2+) channels (HVACCs) in adult dorsal root ganglion (DRG) neurons t...

journal_title：Journal of neurochemistry

authors： Wu ZZ,Cai YQ,Pan HL

更新日期：2009-05-01 00:00:00

abstract：:Mitochondria and releasable endoplasmic reticulum (ER) calcium modulate neuronal calcium signaling, and both change in Alzheimer's disease (AD). The releasable calcium stores in the ER are exaggerated in fibroblasts from AD patients and in multiple models of AD. The activity of the alpha-ketoglutarate dehydrogenase co...

journal_title：Journal of neurochemistry

authors： Chen H,Cross AC,Thakkar A,Xu H,Li A,Paull D,Noggle SA,Kruger L,Denton TT,Gibson GE

更新日期：2020-08-31 00:00:00

abstract：:We have examined the binding of the adenosine agonist radioligands [3N]N6-cyclohexyladenosine ([3H]CHA) and [3H]5'-N-ethylcarboxamidoadenosine ([3H]NECA) to membranes prepared from postmortem human pineal glands. The results showed that the A-1-specific ligand CHA did not bind to membranes. By contrast, [3H]NECA, a no...

journal_title：Journal of neurochemistry

authors： Reynaud D,Gharib A,Lagarde M,Sarda N

更新日期：1990-10-01 00:00:00

abstract：:Hippocampal neurons are affected by chronic stress and have a high density of cytoplasmic mineralocorticoid and glucocorticoid receptors (MR and GR). Detailed studies on the genomic effects of the stress hormone corticosterone at physiologically relevant concentrations on different steps in synaptic transmission are l...

journal_title：Journal of neurochemistry

authors： Chatterjee S,Sikdar SK

更新日期：2014-02-01 00:00:00

abstract：:Previous evidence has suggested that brain catecholamine levels are important in the regulation of central angiotensin II receptors. In the present study, the effects of norepinephrine and 3,4-dihydroxyphenylethylamine (dopamine) on angiotensin II receptor regulation in neuronal cultures from rat hypothalamus and brai...

journal_title：Journal of neurochemistry

authors： Sumners C,Watkins LL,Raizada MK

更新日期：1986-10-01 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) was induced in cultured rat astrocytes by incubation with lipopolysaccharide (LPS) for 18 h and gap junction permeability was assessed by the scrape-loading/Lucifer yellow transfer technique. Induction of NOS was confirmed by determining either the NG-methyl-L-arginine (NMMA)-inhibita...

journal_title：Journal of neurochemistry

authors： Bolaños JP,Medina JM

更新日期：1996-05-01 00:00:00

abstract：:Using a cell culture model of the blood-brain barrier (BBB), we investigated the brain capillary endothelial cell (EC) response to hypoxia. The activities of antioxidant enzymes such as glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase and the GSH level of brain capillary ECs alone or i...

journal_title：Journal of neurochemistry

authors： Plateel M,Dehouck MP,Torpier G,Cecchelli R,Teissier E

更新日期：1995-11-01 00:00:00