Abstract:
:Animals trained in a passive avoidance task exhibit a transient time-dependent increase in hippocampal neural cell adhesion molecule (NCAM) polysialylation at 12-24 h following the initial learning trial. Using immunocytochemical techniques with a monoclonal antibody that specifically recognises NCAM-polysialic acid homopolymers, a distinct population of granule-like cells, at the border of the granule cell layer and the hilus in the dentate gyrus of the adult rat hippocampus, has been demonstrated to exhibit time-dependent change in frequency at 10-12 h following the initial learning of a one-trial, step-through, passive avoidance response. These changes were paradigm specific as they failed to occur in those animals rendered amnesic with scopolamine. These polysialylated dentate neurons are not de novo granule cell precursors as administration of 5-bromo-2'-deoxyuridine every 2 h from the point of learning to the 12-h posttraining time showed no significant difference between trained and passive animals in the small number of heterogeneously distributed, labelled cells. These findings directly identify a morphological substrate of memory, implied by previous correlative and interventive studies on NCAM function.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Fox GB,O'Connell AW,Murphy KJ,Regan CMdoi
10.1046/j.1471-4159.1995.65062796.xsubject
Has Abstractpub_date
1995-12-01 00:00:00pages
2796-9issue
6eissn
0022-3042issn
1471-4159journal_volume
65pub_type
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journal_title:Journal of neurochemistry
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abstract::This study demonstrates that in mammalian and nonmammalian species it is possible to deplete selectively and reversibly retinal glycinergic neurons of their content of glycine by exposure to sarcosine, a competitive inhibitor of glycine transporter 1 (glyt-1). This observation was used as a tool to test the hypothesis...
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journal_title:Journal of neurochemistry
pub_type: 杂志文章
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更新日期:1992-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of neurochemistry
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2006-06-01 00:00:00
abstract::In PC12 cells, preincubated with [3H]inositol, nerve growth factor (NGF) stimulated an approximately 100% increase in the levels of [3H]inositol 1,3,4-trisphosphate ([3H]-Ins(1,3,4)P3], [3H]inositol 1,4,5-trisphosphate ([3H]Ins(1,4,5)P3], and [3H]inositol 1,3,4,5-tetrakisphosphate ([3H]Ins(1,3,4,5)P4] as early as 5-15...
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pub_type: 杂志文章
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