Relationship between in vivo degradative rates and isoelectric points of proteins.

abstract：:It had long been thought, but never demonstrated, that imino acids are formed in the reactions catalyzed by D- and L-amino acid oxidases (EC 1.4.3.3 and 1.4.3.2). The formation of imino acids is now shown directly by allowing the amino acid oxidase reaction to proceed in the presence of NaBH(4), when the imino acid is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hafner EW,Wellner D

更新日期：1971-05-01 00:00:00

abstract：:A recombinant plasmid containing a DNA segment complementary to rat liver albumin mRNA has been constructed, cloned, and used to examine the organization of albumin gene. The 18S fraction of total liver poly(A)-containing RNA was copied into a double-stranded cDNA by avian myeloblastosis virus reverse transcriptase an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kioussis D,Hamilton R,Hanson RW,Tilghman SM,Taylor JM

更新日期：1979-09-01 00:00:00

abstract：:Two cDNAs encoding two human receptors for retinoic acid (RA), RAR-alpha and RAR-beta, have been characterized recently. Synthetic peptides corresponding to the cDNA-deduced amino acid sequences unique to RAR-alpha and RAR-beta were used to generate anti-RAR-alpha antiserum (SP171) and anti-RAR-beta antisera (SP172 an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gaub MP,Lutz Y,Ruberte E,Petkovich M,Brand N,Chambon P

更新日期：1989-05-01 00:00:00

abstract：:Human declarative memory involves a systematic organization of information that supports generalizations and inferences from acquired knowledge. This kind of memory depends on the hippocampal region in humans, but the extent to which animals also have declarative memory, and whether inferential expression of memory de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dusek JA,Eichenbaum H

更新日期：1997-06-24 00:00:00

abstract：:The activation of plant defensive genes in leaves of tomato plants in response to herbivore damage or mechanical wounding is mediated by a mobile 18-amino acid polypeptide signal called systemin. Systemin is derived from a larger, 200-amino acid precursor called prosystemin, similar to polypeptide hormones and soluble...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bergey DR,Howe GA,Ryan CA

更新日期：1996-10-29 00:00:00

abstract：:Notch (N) is a transmembrane receptor that mediates cell-cell interactions to determine many cell-fate decisions. N contains EGF-like repeats, many of which have an O-fucose glycan modification that regulates N-ligand binding. This modification requires GDP-L-fucose as a donor of fucose. The GDP-L-fucose biosynthetic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ayukawa T,Matsumoto K,Ishikawa HO,Ishio A,Yamakawa T,Aoyama N,Suzuki T,Matsuno K

更新日期：2012-09-18 00:00:00

abstract：:We explored the feasibility of designing retroviral vectors that can target human breast cancer cells with characteristic receptors via ligand-receptor interaction. The ecotropic Moloney murine leukemia virus envelope was modified by insertion of sequences encoding human heregulin. Ecotropic virus, which normally does...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han X,Kasahara N,Kan YW

更新日期：1995-10-10 00:00:00

abstract：:The karyophilic properties of the HIV-1 nucleoprotein complex facilitate infection of nondividing cells such as macrophages and quiescent T lymphocytes, and allow the in vivo delivery of transgenes by HIV-derived retroviral vectors into terminally differentiated cells such as neurons. Although the viral matrix (MA) an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallay P,Hope T,Chin D,Trono D

更新日期：1997-09-02 00:00:00

abstract：:The mammalian innate immune system uses several sensors of double-stranded RNA (dsRNA) to develop the interferon response. Among these sensors are dsRNA-activated oligoadenylate synthetases (OAS), which produce signaling 2',5'-linked RNA molecules (2-5A) that activate regulated RNA decay in mammalian tissues. Differen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donovan J,Whitney G,Rath S,Korennykh A

更新日期：2015-03-31 00:00:00

abstract：:Renal cells isolated from vitamin D-deficient chicks had an increased Na+-dependent phosphate uptake when preincubated with 1,25-dihydroxycholecalciferol [1,25-(OH)2D3]. Phosphate uptake in the absence of Na+ and methyl alpha-glucoside uptake dependent on Na+ were not affected. Phosphate uptake was stimulated 15% by 0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang CT,Barnes J,Balakir R,Cheng L,Sacktor B

更新日期：1982-06-01 00:00:00

abstract：:Sensitization of defensive gill and siphon withdrawal reflexes in Aplysia results, in part, from presynaptic facilitation of transmitter release from mechanoreceptor sensory neurons that innervate the siphon skin and synapse with interneurons and motor neurons. Presynaptic facilitation also can be elicited by applicat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hochner B,Klein M,Schacher S,Kandel ER

更新日期：1986-11-01 00:00:00

abstract：:The fractionation of cells of a parsley suspension culture [Petroselinum crispum (Mill.) A. Hill] by protoplasting and subsequent removal of the vacuoles led to physiologically intact evacuolated protoplasts retaining light inducibility of chalcone synthase expression. Lysis of the evacuolated protoplasts permitted th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harter K,Frohnmeyer H,Kircher S,Kunkel T,Mühlbauer S,Schäfer E

更新日期：1994-05-24 00:00:00

abstract：:The Hippo pathway is an evolutionarily conserved regulator of organ growth and tumorigenesis. In Drosophila, oncogenic RasV12 cooperates with loss-of-cell polarity to promote Hippo pathway-dependent tumor growth. To identify additional factors that modulate this signaling, we performed a genetic screen utilizing the D...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo X,Sun Y,Azad T,Janse van Rensburg HJ,Luo J,Yang S,Liu P,Lv Z,Zhan M,Lu L,Zhou Y,Ma X,Zhang X,Yang X,Xue L

更新日期：2020-12-01 00:00:00

abstract：:Two genes encoding steroid 21-hydroxylase [21-OHase; steroid 21-monooxygenase; steroid, hydrogen-donor: oxygen oxidoreductase (21-hydroxylating); EC 1.14.99.10], a cytochrome P-450 enzyme, have been located within the HLA major histocompatibility complex. Congenital adrenal hyperplasia due to 21-OHase deficiency is a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White PC,Grossberger D,Onufer BJ,Chaplin DD,New MI,Dupont B,Strominger JL

更新日期：1985-02-01 00:00:00

abstract：:A significant improvement in molecular hydrogen uptake properties is revealed by our ab initio calculations for Li-decorated metal-organic framework 5. We have found that two Li atoms are strongly adsorbed on the surfaces of the six-carbon rings, one on each side, carrying a charge of +0.9e per Li atom. Each Li can cl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blomqvist A,Araújo CM,Srepusharawoot P,Ahuja R

更新日期：2007-12-18 00:00:00

abstract：:The electron spin resonance spectrum of the triplet excited state of Rhodopseudomonas spheroides R-26 reaction centers has been studied after excitation with the polarized narrow-bandwidth output of a tunable dye laser from 520 to 670 nm. A theory is developed relating experimental observables to the angles between th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boxer SG,Roelofs MG

更新日期：1979-11-01 00:00:00

abstract：:The Clausius-Mossotti function for the dielectric constant is expanded in terms of single atom and pair polarizabilities, leading to contributions that depend on both the trace and the anisotropy of the pair-polarizability tensor. The short-range contribution of the anisotropic part to the pair polarizabilities has pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alder BJ,Beers JC,Strauss HL,Weis JJ

更新日期：1980-06-01 00:00:00

abstract：:Water dynamics in concentrated ionic solutions plays an important role in a number of material and energy conversion processes such as the charge transfer at the electrolyte-electrode interface in aqueous rechargeable ion batteries. One long-standing puzzle is that all electrolytes, regardless of their "structure-maki...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Q,Wu T,Chen C,Mukamel S,Zhuang W

更新日期：2017-09-19 00:00:00

abstract：:Intergroup conflict is a persistent feature of many human societies yet little is known about why individuals participate when doing so imposes a mortality risk. To evaluate whether participation in warfare is associated with reproductive benefits, we present data on participation in small-scale livestock raids among ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glowacki L,Wrangham R

更新日期：2015-01-13 00:00:00

abstract：:In the last decade, a number of quantitative epidemiological studies of specific diseases have been done in developing countries that for the first time allow estimation of the total burden of disease (mortality and morbidity) attributable to use of solid fuels in adult women and young children, who jointly receive th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith KR

更新日期：2000-11-21 00:00:00

abstract：:Phosphorylation plays a critical role in controlling the function of cytoskeletal assemblies but no direct method yet exists to measure the phosphorylation state of proteins at the level of individual molecules and assemblies. Herein, we apply scanning transmission electron microscopy in combination with electron ener...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leapman RD,Gallant PE,Reese TS,Andrews SB

更新日期：1997-07-22 00:00:00

abstract：:A study was made of the effects of di- and trivalent cations on homomeric rho 1-type gamma-aminobutyrate (GABA rho 1) receptors expressed in Xenopus oocytes after injection of mRNA coding for the GABA rho 1 subunit. GABA elicited large currents with a Kd approximately 1 microM. The properties of these GABA rho 1 recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calvo DJ,Vazquez AE,Miledi R

更新日期：1994-12-20 00:00:00

abstract：:Vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs) are key drivers of blood and lymph vessel formation in development, but also in several pathological processes. VEGF-C signaling through VEGFR-3 promotes lymphangiogenesis, which is a clinically relevant target for treating lymphatic insufficienc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leppänen VM,Tvorogov D,Kisko K,Prota AE,Jeltsch M,Anisimov A,Markovic-Mueller S,Stuttfeld E,Goldie KN,Ballmer-Hofer K,Alitalo K

更新日期：2013-08-06 00:00:00

abstract：:Several overlapping chromosomal deletions spanning the albino locus in the mouse cause perinatal lethality when homozygous and a block in the transcriptional induction of various unlinked hepatocyte-specific genes. Studies of such lethal albino deletion homozygotes in perinatal stages revealed a deficiency in the tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaret KS,Milos P,Lia M,Bali D,Gluecksohn-Waelsch S

更新日期：1992-07-15 00:00:00

abstract：:Specific rabbit antisera against purified bovine pituitary carboxypeptidase processing enzyme (which has also been referred to as "enkephalin convertase") have been prepared and characterized. The antisera recognized both the purified soluble and the membrane-bound forms of the enzyme with equal affinity, suggesting t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hook VY,Mezey E,Fricker LD,Pruss RM,Siegel RE,Brownstein MJ

更新日期：1985-07-01 00:00:00

abstract：:In the T-cell somatic mutant J.CaM1, the T-cell antigen receptor complex is poorly coupled to the inositolphospholipid second messenger system; some antibodies against the invariant CD3 subunit of the receptor retain their agonist function in J.CaM1. Here we show by a combination of complementation assays that the mut...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldsmith MA,Dazin PF,Weiss A

更新日期：1988-11-01 00:00:00

abstract：:The antigenic sites of horse and rat cytochromes c in the major antigenic region were compared using a panel of variant cytochromes c and a large number of BALB/c mouse monoclonal antibodies (mAbs) in competitive ELISAs. The major antigenic region of cytochrome c is located on the surface opposite of that containing t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jemmerson R,Johnson JG

更新日期：1991-05-15 00:00:00

abstract：:H-NS and Lsr2 are nucleoid-associated proteins from Gram-negative bacteria and Mycobacteria, respectively, that play an important role in the silencing of horizontally acquired foreign DNA that is more AT-rich than the resident genome. Despite the fact that Lsr2 and H-NS proteins are dissimilar in sequence and structu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon BR,Li Y,Cote A,Weirauch MT,Ding P,Hughes TR,Navarre WW,Xia B,Liu J

更新日期：2011-06-28 00:00:00

abstract：:The importance of identifying VEGF-independent pathways in pathological angiogenesis is increasingly recognized as a result of the emerging drug resistance to anti-VEGF therapies. PDGF-CC is the third member of the PDGF family discovered after more than two decades of studies on PDGF-AA and PDGF-BB. The biological fun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hou X,Kumar A,Lee C,Wang B,Arjunan P,Dong L,Maminishkis A,Tang Z,Li Y,Zhang F,Zhang SZ,Wardega P,Chakrabarty S,Liu B,Wu Z,Colosi P,Fariss RN,Lennartsson J,Nussenblatt R,Gutkind JS,Cao Y,Li X

更新日期：2010-07-06 00:00:00

abstract：:The liver provides for long-term energy needs of the body by converting excess carbohydrate into fat for storage. Insulin is one factor that promotes hepatic lipogenesis, but there is increasing evidence that glucose also contributes to the coordinated regulation of carbohydrate and fat metabolism in liver by mechanis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iizuka K,Bruick RK,Liang G,Horton JD,Uyeda K

更新日期：2004-05-11 00:00:00