Abstract：
OBJECTIVE:To detect 3 kinds of N-ras mutations in Chinese patients with acute myeloid leukemia (AML). METHODS:In vitro DNA amplification followed by oligonucleotide dot analysis were used to study N-ras gene mutations in 43 cases of acute myeloid leukemia (AML). 25 healthy people were used as controls. Patients were selected in the Beijing district and consisted of 19 males and 24 females. The average age was 37. The controls were healthy individuals with the average age of 36.5 from the same region. 3 oligonucleotide probes were artificially synthesized to detect mutations in codon 12 and 13 of N-ras. RESULTS:Five out of 43 AML samples have been found contain G-->A mutation in codon 12.2 have G-->T mutation in codon 12. One has G-->A mutation in codon 13. The mutation rate was 18.6%. None of the controls presented these mutations. The frequency of mutation of N-ras in the AML samples showed statistical differences with that of the controls. CONCLUSION:Analysis of the results suggests the N-ras mutations may have some relationship with the etiology of acute myeloid leukemia.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Guo W,Tang B,Xu S,Yao Y,Ye Dkeywords:
subject
Has Abstractpub_date
1998-04-01 00:00:00pages
343-5issue
4eissn
0366-6999issn
2542-5641journal_volume
111pub_type
杂志文章abstract：BACKGROUND:There are more than 300 genetic loci that have been found to be related to hereditary hearing impairment (HHI), including 92 causative genes for nonsyndromic hearing loss, among which 34 genes are related to autosomal dominant nonsyndromic HHI (ADNSHHI). Traditional linkage analysis and candidate gene sequen...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.171440
更新日期：2015-12-20 00:00:00
abstract：OBJECTIVE:This article aims to present a brief profile of the advances in prevention and treatment of myocardial injury in cancer therapy based on relevant literature or reports. DATA SOURCES:The data cited in this review were obtained from articles indexed in PubMed and China National Knowledge Internet (CNKI) up to ...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.1097/CM9.0000000000000498
更新日期：2019-11-20 00:00:00
abstract：:Ninety-five patients with perforation, hemorrhage or stenosis due to duodenal ulcer were treated by extended parietal cell vagotomy. Postoperative follow-up ranged from 3.5 to 10 years (mean 6 years) in 88 patients (92%) with acute perforation (60), hemorrhage (8) and stenosis (20). There was no operative mortality. U...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：BACKGROUND:The controversy on the treatment strategy for severe acute pancreatitis (SAP) has never stopped for the past century. Even now surgical procedures play a decisive role in the treatment of SAP, especially in managing the related complications, but the rational indications, timing, and approaches of surgical i...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Using tissue Doppler imaging and conventional echocardiographic technique, we examined the cardiac function and synchronicity in individuals with isolated right bundle branch block (RBBB) or left bundle branch block (LBBB) and assessed the relationship between QRS duration and synchronicity. METHODS:Subject...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-05-20 00:00:00
abstract：:Recombinant DNA Yeast-Derived Hepatitis B Vaccine (RYHB vaccine) is comparable to and can replace Plasma-Derived Hepatitis B Vaccine (PHB vaccine) for the prevention of mother-infant transmission of hepatitis B virus (HBV), but the duration of immune efficacy of RYHB vaccine is not clear. This study indicates the long...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1994-12-01 00:00:00
abstract：OBJECTIVE:To evaluate the incidence, severity, clinical manifestations and immunological features relevant to liver involvement in 135 cases of primary Sjögren's syndrome. METHODS:One hundred and thirty-five patients with definite primary Sjögren's syndrome were analyzed retrospectively for liver involvement by the ab...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Induced pluripotent stem (iPS) cells are a recent development which has brought a promise of great therapeutic values. The previous technique of somatic cell nuclear transfer (SCNT) has been ineffective in humans. Recent discoveries show that human fibroblasts can be reprogrammed by a transient over expression of a sm...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：BACKGROUND:Ulcerative colitis (UC) is associated with differential expression of genes involved in inflammation and tissue remodeling. MicroRNA (miRNA) plays an important role in the pathogenesis of UC by regulating the gene expression at the post-transcriptional level and control crucial physiological processes. This ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:As a new electroencephalogram (EEG) signal processing technique for monitoring the depth of anesthesia, entropy consists of two indices: reaction entropy (RE) and state entropy (SE). Our study compared entropy with classical bispectral index (BIS) in reduction of myoelectrical interference and noxious stimul...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：2012-04-01 00:00:00
abstract：OBJECTIVE:To study the prevalence of skin positivity to purified protein derivative (PPD) in human immunodeficiency virus (HIV)-infected patients in Hong Kong. METHODS:Consecutive clients of an HIV clinic were administered the PPD test and 2 units of PPD-RT23 were used. The area of induration was then measured in 48 t...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-07-01 00:00:00
abstract：OBJECTIVE:To investigate the protective effect of dl-3-n-butylphthalide (NBP) as an anti-cerebral ischemic drug on brain damage 24 h after closed head injury in mice. METHODS:Closed head injury was induced by dropping a 50-g weight from a height of 18 cm on a metal impounder resting on the parietal bone in mice. RESU...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：OBJECTIVE:To clarify whether genetic variants of the renin-angiotensin system (RAS) contribute to the development of diabetic nephropathy (DN) in the Chinese. METHODS:Totally 173 Chinese subjects of Han nationality from Shanghai were classified into! control, DN (-) and DN (+) groups. The latter was subdivided accordi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：BACKGROUND:The endovascular treatment of abdominal aortic aneurysm (AAA) has improved greatly in the last 15 years. The present study aimed to evaluate the endografting experience for the treatment of unfavorable abdominal aortic aneurysm (uAAA). METHODS:During December 2001 and December 2007, 41 patients with uAAA we...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2009-04-05 00:00:00
abstract：BACKGROUND:Diffusion tensor imaging (DTI) permits quantitative examination within the pyramidal tract (PT) by measuring fractional anisotropy (FA). To the best of our knowledge, the inter-variability measures of FA along the PT remain unexplained. A clear understanding of these reference values would help radiologists ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-06-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of Q-switched alexandrite laser irradiation on dermal melanocytes of nevus of Ota. METHODS:Multiple biopsies were carried out on 4 patients with nevus of Ota before and after laser irradiation. Altogether 11 samples were examined under light microscope and 14 under transmission elec...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：OBJECTIVE:To focus on the asthmatic pathogenesis and clinical manifestations related to epithelial sodium channel (ENaC)/chlorine ion channel. DATA SOURCES:The data analyzed in this review were the English articles from 1980 to 2015 from journal databases, primarily PubMed and Google Scholar. The terms used in the lit...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.162494
更新日期：2015-08-20 00:00:00
abstract：BACKGROUND:Gastric stromal tumors arising from the muscularis propria are located in deeper layers. Endoscopic resection may be contraindicated due to the possibility of perforation. These tumors are therefore usually removed by surgical or laparoscopic procedures. This study evaluated the curative effects, safety and ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.157651
更新日期：2015-06-05 00:00:00
abstract：OBJECTIVE:To review articles aiming to present an overview of the principles, progress, uses and limitations of laser speckle flowgraphy (LSFG) in posterior fundus circulation research. DATA SOURCES:The data used in this review was obtained mainly from the studies reported in PubMed using the key terms "laser speckle"...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVE:To review the research progress on Type IV secretion system (T4SS) in Helicobacter pylori. DATA SOURCES:The data used in this review were identified by searching of PUBMED (1995 - 2007) online resources using the key terms 'Type IV secretion system' and 'Helicobacter pylori'. STUDY SELECTION:Mainly original...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2007-12-05 00:00:00
abstract：BACKGROUND:This retrospective study aimed to investigate the difference of the intra-dural reserving space for spinal cord in magnetic resonance imaging (MRI) between patients with and without developmental cervical stenosis and its clinical significance. METHODS:A total of 264 patients with cervical spondylotic myelo...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Candidal esophagitis is the primary infection among all digestive tract opportunistic ones in acquired immunodeficiency syndrome (AIDS) cases. X-ray manifestation reports of it are still rare. This study aimed to conduct a retrospective analysis on the X-ray data of 6 AIDS cases complicated with candidal eso...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
abstract：BACKGROUND:In patients with chronic total occlusion (CTO) and multivessel coronary artery disease, the comparison of surgical and the percutaneous revascularization strategies has rarely been conducted. The aim of this study was to compare long term clinical outcomes of drug eluting stent (DES) implantation with corona...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
abstract：OBJECTIVE:To investigate the regulation effect of protein kinase ERK on the activation of transcription factor STAT3 in response to IL-6 in the Sko-007 human myeloma cell line. METHODS:Electrophoretic mobility shift assay (EMSA) and immunoprecipitation (IP) were used to show the activation of STAT3 and ERK in Sko-007 ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：OBJECTIVE:To investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial cavity injection (ICI). METHODS:Fifteen dogs were used, and 0.3 ml (1 x 10(9) pfu) of an adenovirus (Ad...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-04-01 00:00:00
abstract：BACKGROUND:Retroperitoneal sarcoma is a rare disease with poor prognosis. The aim of this study was to investigate the prognostic factors of the disease. METHODS:Between January 1988 and December 2003, 132 patients with retroperitoneal sarcoma were surgically treated in our hospital, 79 of them were followed up for 1 ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2007-06-20 00:00:00
abstract：:Aluminium citrate (Al-cit) was shown to be biologically effective in preventing silicosis. A total of 917 cases selected from both engineering corps of railway and coalmines with stage 0 or 0+ by radiographs were studied. Cases were randomly divided into Al-cit treatment group and control group with continuing dust ex...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1990-02-01 00:00:00
abstract：BACKGROUND:The Dynamic Interspinous Assisted Motion (DIAM) system was designed to stabilize degenerative spinal segments without fusion surgery, maintain segment motion and prevent adjacent segment degeneration. The aim of this study was to investigate clinical efficacy of the DIAM system in treatment of degenerative l...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2010-11-01 00:00:00
abstract：BACKGROUND:The efficacy of percutaneous intradiscal radiofrequency thermocoagulation (PIRFT) for the treatment of discogenic low back pain (LBP) remains controversial. However, all the PIRFT studies utilized monopolar radiofrequency thermocoagulation (RFTC). The aim of this study was to investigate the safety and effic...
journal_title：Chinese medical journal
pub_type： 临床试验,杂志文章
doi：10.4103/0366-6999.190682
更新日期：2016-10-05 00:00:00
abstract：BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) pneumonia is an important issue with significant morbidity and mortality in clinical practice, especially in diabetes mellitus (DM). Studies focusing on S. aureus pneumonia in DM is limited, we sought to make a relatively comprehensive exploration of clinica...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000270
更新日期：2019-06-20 00:00:00
