Abstract：
OBJECTIVE:To detect 3 kinds of N-ras mutations in Chinese patients with acute myeloid leukemia (AML). METHODS:In vitro DNA amplification followed by oligonucleotide dot analysis were used to study N-ras gene mutations in 43 cases of acute myeloid leukemia (AML). 25 healthy people were used as controls. Patients were selected in the Beijing district and consisted of 19 males and 24 females. The average age was 37. The controls were healthy individuals with the average age of 36.5 from the same region. 3 oligonucleotide probes were artificially synthesized to detect mutations in codon 12 and 13 of N-ras. RESULTS:Five out of 43 AML samples have been found contain G-->A mutation in codon 12.2 have G-->T mutation in codon 12. One has G-->A mutation in codon 13. The mutation rate was 18.6%. None of the controls presented these mutations. The frequency of mutation of N-ras in the AML samples showed statistical differences with that of the controls. CONCLUSION:Analysis of the results suggests the N-ras mutations may have some relationship with the etiology of acute myeloid leukemia.
journal_name
Chin Med J (Engl)journal_title
Chinese medical journalauthors
Guo W,Tang B,Xu S,Yao Y,Ye Dkeywords:
subject
Has Abstractpub_date
1998-04-01 00:00:00pages
343-5issue
4eissn
0366-6999issn
2542-5641journal_volume
111pub_type
杂志文章abstract：BACKGROUND:To avoid the irritation of tendons and soft tissues as well as hardware-related problems, we designed an intramedullary fixation with bioabsorbable rods for the treatment of the metacarpal shaft fractures. METHODS:Five patients with nine shaft fractures of the fourth and fifth metacarpi were treated with in...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.168040
更新日期：2015-11-05 00:00:00
abstract：:Myocardial revascularization by Nd: YAG laser used in rats with myocardial infarction was studied. The left coronary artery was ligated and the laser channels with a diameter of 300 microns were made immediately by 2.5-4.2 W lasersonics YAG unit. After 24 hours, the rats were killed. Evaluation included hemodynamics, ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：BACKGROUND:A satisfactory animal model of breast cancer metastasizing to bone is unavailable. In this study, we used human breast cancer stem-like cells and human bone to build a novel "human-source" model of human breast cancer skeletal metastasis. METHODS:Human breast cancer stem-like cells, the CD44+/CD24-/lower su...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-10-20 00:00:00
abstract：OBJECTIVE:To review the recent developments in the mechanisms of glucocorticoids induced osteonecrosis of femoral head (ONFH) and introduce a new theory of ONFH. DATA SOURCES:Both Chinese- and English-language literatures were searched using MEDLINE (1997 - 2011), Pubmed (1997 - 2011) and the Index of Chinese-language...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:To investigate the antitumor immune efficiency of mouse dendritic cells (mDCs) by using adenovirus-mediated tumor necrosis factor-alpha (AdV-TNF-alpha) gene transfer. METHODS:MDCs infected with AdV-TNF-alpha and AdV-pLpA (no gene insert) at 100 multiplicity of infection (MOI) were analyzed by RNase protectio...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：OBJECTIVE:To report our experiences in retroperitoneoscopic renal pedicle lymphatic stripping for chyluria. METHODS:Six cases of filarial chyluria were admitted to our hospital from November 2001 to June 2002. Of these cases, 4 were men and 2 women, with age ranging from 34 to 52 years (mean, 42 years). Diagnosis was ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：BACKGROUND:Acute lung injury (ALI) is a serious and common condition for which there are currently no specific strategies for treatment. Recent studies have suggested that bone marrow-derived multipotent mesenchymal stem cells (MSCs) may have therapeutic applications in multiple clinical disorders. We explored the biol...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-09-01 00:00:00
abstract：BACKGROUND:Patients with elevated admission glucose levels may be at increased risk of death after myocardial infarction, independent of other baseline risk factors and more severe coronary artery disease. However, data regarding admission glucose and epicardial and microvascular flow after primary angioplasty is limit...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2006-01-20 00:00:00
abstract：BACKGROUND:The molecular mechanisms of Shenxianshengmai (SXSM), a traditional Chinese medicine, on bradycardia have been incompletely understood. The study tried to investigate the gene expression profile and proteomics of bradycardia rabbits' hearts after SXSM treatment. METHODS:Twenty-four adult rabbits were randoml...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.197999
更新日期：2017-01-20 00:00:00
abstract：BACKGROUND:Early diagnosis and accurate staging are important to improve the cure rate and prognosis for pancreatic cancer. This study was performed to develop an automatic and accurate imaging processing technique system, allowing this system to read computed tomography (CT) images correctly and make diagnosis of panc...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.1097/CM9.0000000000000544
更新日期：2019-12-05 00:00:00
abstract：:The ultrastructural changes of chronic cardiopathy in diabetic biobreeding rats were observed electronmicroscopically. The thickness of the myocardial capillary basement membrane (CBM) and volume fraction of myofilaments in the myocardium were quantitatively measured with a video interactive display system III (VIDS-I...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：OBJECTIVE:To explore the association of human leukocyte antigen (HLA) with pregnancy induced hypertension (PIH). METHODS:We oligotyped HLA-DQA1, -DQB1 locus of 30 Chinese PIH families and 14 control families in Shanghai area by polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO) hybridization method ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：OBJECTIVES:To get a more comprehensive recognition about carpal tunnel syndrome (CTS), especially the manual housework as its risk factor, and to facilitate early diagnosis and proper treatment. METHODS:262 CTS patients (396 CTS hands) were analyzed retrospectively. A 1:1 matched case-control study of 61 woman pairs i...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Gastric stromal tumors arising from the muscularis propria are located in deeper layers. Endoscopic resection may be contraindicated due to the possibility of perforation. These tumors are therefore usually removed by surgical or laparoscopic procedures. This study evaluated the curative effects, safety and ...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.157651
更新日期：2015-06-05 00:00:00
abstract：Background:Endometriosis is a challenging disease with symptoms such as dysmenorrhea and infertility. However, its etiology is still vague and there is still no effective markers or treatment. This study aimed to profile the circular RNAs (circRNAs) expressed in eutopic endometrium from patients with ovarian endometrio...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.226070
更新日期：2018-03-05 00:00:00
abstract：OBJECTIVE:To focus on the asthmatic pathogenesis and clinical manifestations related to epithelial sodium channel (ENaC)/chlorine ion channel. DATA SOURCES:The data analyzed in this review were the English articles from 1980 to 2015 from journal databases, primarily PubMed and Google Scholar. The terms used in the lit...
journal_title：Chinese medical journal
pub_type： 杂志文章,评审
doi：10.4103/0366-6999.162494
更新日期：2015-08-20 00:00:00
abstract：BACKGROUND:The effects of near-road pollution on lung function in China have not been well studied. We aimed to investigate the effects of long-term exposure to traffic-related air pollution on lung function, airway inflammation, and respiratory symptoms. METHODS:We enrolled 1003 residents aged 57.96 ± 8.99 years livi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.189923
更新日期：2016-09-20 00:00:00
abstract：BACKGROUND:miR-338-3p is a recently discovered miRNA and is involved in cell differentiation. However, few data are yet available on the aberrant expression of miR-338-3p in human colorectal carcinoma (CRC). This work aimed to investigate the relationship between miR-338-3p expression pattern and clinicopathological fe...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:Acute fatty liver of pregnancy (AFLP) is a rare but life-threatening complication occurring in the third trimester. It is often fatal to both mother and fetus. The complicated clinical manifestations as well as an insufficient understanding of the disease make the precise diagnosis and effective treatment of...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.181963
更新日期：2016-05-20 00:00:00
abstract：BACKGROUND:Bone grafting is commonly used to repair bone defects. As the porosity of the graft scaffold increases, bone formation increases, but the strength decreases. Early attempts to engineer materials were not able to resolve this problem. In recent years, nanomaterials have demonstrated the unique ability to impr...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：BACKGROUND:It is unclear whether a history of paroxysmal atrial fibrillation (PAF) would impact the effect of catheter ablation on persistent atrial fibrillation (AF). This study aimed to compare the effect of catheter ablation on persistent AF with and without a history of PAF. METHODS:One hundred and eighty-three pa...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND:Von Hippel-Lindau (VHL) disease is a hereditary tumor disorder caused by mutations or deletions of the VHL gene. Few studies have documented the clinical phenotype and genetic basis of the occurrence of VHL disease in China. This study armed to present clinical and genetic analyses of VHL within a five-gener...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：10.4103/0366-6999.147802
更新日期：2015-01-05 00:00:00
abstract：BACKGROUND:Imaging-guided thermal ablation using different energy sources continues to gain favor as a minimally invasive technique for the treatment of primary and metastatic hepatic malignant tumors. This study aimed to evaluate the performance of microwave ablation with 2450-MHz internally cooled-shaft antenna in ex...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：BACKGROUND:Type 1 diabetes has been recognized as an organ specific autoimmune disease owing to the immune destruction of pancreatic islet beta cells in genetically susceptible individuals. In both human and rodent models of type 1 diabetes, such as nonobese diabetic (NOD) mice, biobreeding rats, the disease has a dist...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-08-05 00:00:00
abstract：BACKGROUND:Acinetobacter baumannii is one of the main gram-negative bacilli in clinical practice. Nosocomial infections caused by multi-drug resistance Acinetobacter baumannii is very difficult to treat. This study was designed to investigate the antimicrobial resistance characteristics and four resistant gene expressi...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2005-01-20 00:00:00
abstract：BACKGROUND:Transcription factors hypoxia inducible factor 1alpha (HIF 1alpha) and endothelial PAS domain protein 1 (EPAS1) promote the transcription of vascular endothelial growth factor (VEGF). VEGF enhances angiogenesis and vascular permeability of tumours, which promotes tumour growth and facilitates entry of cancer...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2008-11-20 00:00:00
abstract：BACKGROUND:It is the first multicenter clinical study in China to investigate zanamivir use among Chinese adolescents and adults with influenza-like illness (ILI) since 2009, when inhaled zanamivir (RELENZA(®)) was marketed in China. METHODS:An uncontrolled open-label, multicentre study to evaluate the antiviral activ...
journal_title：Chinese medical journal
pub_type： 杂志文章,多中心研究
doi：
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVES:To investigate the effect of Burn Healing Liquid (BHL) on the proliferation of keratinocytes and fibroblasts and to explore the potential effect of BHL on fibroblast contraction. METHODS:Human keratinocytes and dermal fibroblasts were cultured in media containing serial dilutions of BHL followed by cell pro...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：BACKGROUND:Vascular rings are uncommon anomalies in which preferred strategies for diagnosis and management may vary among institutions. In this study, we reported our approach and a review of our 5-year experience. METHODS:From May 2006 to April 2011, 45 children (31 boys) with vascular rings underwent surgical repai...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2012-04-01 00:00:00
abstract：BACKGROUND:A free running asthma screening test is usually used for screening exercise-induced bronchoconstriction. The total serum allergen IgE level can reveal the patient's atopy characteristics. Our study is to evaluate the diagnostic values of the combination of the two tests in asthmatic children and compare this...
journal_title：Chinese medical journal
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
