Ectopic expression of prion protein (PrP) in T lymphocytes or hepatocytes of PrP knockout mice is insufficient to sustain prion replication.

abstract：:PTP1C, a protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48) containing two src homology 2 domains, is poorly active when assayed with various protein substrates in vitro. Its activity is stimulated > 1000-fold by anionic phospholipids when myelin basic protein or mitogen-activated ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Z,Shen SH,Fischer EH

更新日期：1993-05-01 00:00:00

abstract：:Membrane-integrated type II phosphatidic acid phosphatases (PAP2s) are important for numerous bacterial to human biological processes, including glucose transport, lipid metabolism, and signaling. Escherichia coli phosphatidylglycerol-phosphate phosphatase B (ecPgpB) catalyzes removing the terminal phosphate group fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fan J,Jiang D,Zhao Y,Liu J,Zhang XC

更新日期：2014-05-27 00:00:00

abstract：:The transforming growth factors beta (TGF-beta s) are important modulators of growth and differentiation. They are intermolecular disulfide-bonded homodimeric molecules. The monomer fold has a conserved cystine knot and lacks a hydrophobic core. The biological specificity of a given member of the family is believed to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Venkataraman G,Sasisekharan V,Cooney CL,Langer R,Sasisekharan R

更新日期：1995-06-06 00:00:00

abstract：:We report a machine-learning strategy for design of organic structure directing agents (OSDAs) for zeolite beta. We use machine learning to replace a computationally expensive molecular dynamics evaluation of the stabilization energy of the OSDA inside zeolite beta with a neural network prediction. We train the neural...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daeyaert F,Ye F,Deem MW

更新日期：2019-02-26 00:00:00

abstract：:Embryonic stem cell-based therapies exhibit great potential for the treatment of Parkinson's disease (PD) because they can significantly rescue PD-like behaviors. However, whether the transplanted cells themselves release dopamine in vivo remains elusive. We and others have recently induced human embryonic stem cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang X,Xu H,Teng S,Zhang X,Deng Z,Zhou L,Zuo P,Liu B,Liu B,Wu Q,Wang L,Hu M,Dou H,Liu W,Zhu F,Li Q,Guo S,Gu J,Lei Q,Lü J,Mu Y,Jin M,Wang S,Jiang W,Liu K,Wang C,Li W,Zhang K,Zhou Z

更新日期：2014-11-04 00:00:00

abstract：:Using currently available gene fusion techniques, we have constructed plasmids that direct the overproduction of the carboxyl-terminal two-thirds of DNA polymerase I, corresponding to the proteolytically derived "Klenow fragment." We have obtained overproduction amounting to several percent of the cellular protein usi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joyce CM,Grindley ND

更新日期：1983-04-01 00:00:00

abstract：:During mitochondrial ATP synthesis, F1-ATPase-the portion of the ATP synthase that contains the catalytic and regulatory nucleotide binding sites-undergoes a series of concerted conformational changes that couple proton translocation to the synthesis of the high levels of ATP required for cellular function. In the str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bianchet MA,Hullihen J,Pedersen PL,Amzel LM

更新日期：1998-09-15 00:00:00

abstract：:We define an integer invariant [Formula: see text] of a smooth, compact, closed 4-manifold X by minimizing a certain complexity of a trisection of X over all trisections. The good feature of [Formula: see text] is that when [Formula: see text] and X is a homology 4-sphere, then X is diffeomorphic to the 4-sphere. Natu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirby R,Thompson A

更新日期：2018-10-23 00:00:00

abstract：:Kruppel-like factor 4 (KLF4) is involved in self-renewal of embryonic stem cells and reprogramming of somatic cells to pluripotency. However, its role in lineage-committed stem cells remains largely unknown. Here, we show that KLF4 is expressed in neural stem cells (NSCs) and is down-regulated during neuronal differen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qin S,Liu M,Niu W,Zhang CL

更新日期：2011-12-27 00:00:00

abstract：:The repair of DNA double-strand breaks (DSBs) requires open, flexible chromatin domains. The NuA4-Tip60 complex creates these flexible chromatin structures by exchanging histone H2A.Z onto nucleosomes and promoting acetylation of histone H4. Here, we demonstrate that the accumulation of H2A.Z on nucleosomes at DSBs is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gursoy-Yuzugullu O,Ayrapetov MK,Price BD

更新日期：2015-06-16 00:00:00

abstract：:After pollen grains germinate on the stigma, pollen tubes traverse the extracellular matrix of the style on their way to the ovules. We previously characterized two pollen-specific, receptor-like kinases, LePRK1 and LePRK2, from tomato (Lycopersicon esculentum). Their structure and immunolocalization pattern and the s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wengier D,Valsecchi I,Cabanas ML,Tang WH,McCormick S,Muschietti J

更新日期：2003-05-27 00:00:00

abstract：:Circadian pacemaking requires the orderly synthesis, posttranslational modification, and degradation of clock proteins. In mammals, mutations in casein kinase 1 (CK1) epsilon or delta can alter the circadian period, but the particular functions of the WT isoforms within the pacemaker remain unclear. We selectively tar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meng QJ,Maywood ES,Bechtold DA,Lu WQ,Li J,Gibbs JE,Dupré SM,Chesham JE,Rajamohan F,Knafels J,Sneed B,Zawadzke LE,Ohren JF,Walton KM,Wager TT,Hastings MH,Loudon AS

更新日期：2010-08-24 00:00:00

abstract：:Clinically isolated syndrome (CIS) refers to the earliest clinical manifestation of multiple sclerosis (MS). Currently there are no prognostic biological markers that accurately predict conversion of CIS to clinically definite MS (CDMS). Furthermore, the earliest molecular events in MS are still unknown. We used micro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Corvol JC,Pelletier D,Henry RG,Caillier SJ,Wang J,Pappas D,Casazza S,Okuda DT,Hauser SL,Oksenberg JR,Baranzini SE

更新日期：2008-08-19 00:00:00

abstract：:Soluble extracts of adenovirus-infected HeLa cell nuclei support DNA replication on exogenous adenovirus DNA templates. Conditions of synthesis using both wild-type and temperature-sensitive extracts have been defined. Nuclear extracts prepared from cells permissively infected with the adenovirus mutant H5ts125 expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaplan LM,Ariga H,Hurwitz J,Horwitz MS

更新日期：1979-11-01 00:00:00

abstract：:The initiator RNAs of mammalian Okazaki fragments are thought to be removed by RNase HI and the 5'-3' flap endonuclease (FEN1). Earlier evidence indicated that the cleavage site of RNase HI is 5' of the last ribonucleotide at the RNA-DNA junction on an Okazaki substrate. In current work, highly purified calf RNase HI ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murante RS,Henricksen LA,Bambara RA

更新日期：1998-03-03 00:00:00

abstract：:A full-length placental alkaline phosphatase (PLAP) cDNA was used to identify and clone the PLAP-like Nagao isozyme gene from human genomic libraries. The entire nucleotide sequence of the gene reveals the existence of 11 exons interrupted by 10 small introns (76-427 base pairs). Putative regulatory sequences have bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millán JL,Manes T

更新日期：1988-05-01 00:00:00

abstract：:Normal neural development is essential for the formation of neuronal networks and brain function. Cutaneous T cell lymphoma-associated antigen 5 (cTAGE5)/meningioma expressed antigen 6 (MEA6) plays a critical role in the secretion of proteins. However, its roles in the transport of nonsecretory cellular components and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang F,Wang Y,Wang T,Yao L,Lam SM,Huang X,Fan J,Wang Q,Liu L,Jiang Y,Zhang H,Shi L,Yu M,Shui G,Wang Y,Gao F,Zhang X,Xu Z

更新日期：2018-10-02 00:00:00

abstract：:CCAAT/enhancer-binding protein δ (C/EBPδ) recently emerged as an essential player in the inflammatory response to bacterial infections. C/EBPδ levels increase rapidly after a proinflammatory stimulus, and increasing C/EBPδ levels seem to be indispensable for amplification of the inflammatory response. Here we aimed to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duitman J,Schouten M,Groot AP,Borensztajn KS,Daalhuisen JB,Florquin S,van der Poll T,Spek CA

更新日期：2012-06-05 00:00:00

abstract：:Effects of opiate receptor antagonists on centrally mediated cardiovascular responses to clonidine and beta-endorphin were studied in urethane-anesthetized spontaneously hypertensive Okamoto-Aoki rats (SHR), normotensive Sprague-Dawley rats, and Sprague-Dawley rats made hypertensive with deoxycorticosterone pivalate/s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mosqueda-Garcia R,Kunos G

更新日期：1987-12-01 00:00:00

abstract：:The Kitaev model with an applied magnetic field in the [Formula: see text] direction shows two transitions: from a nonabelian gapped quantum spin liquid (QSL) to a gapless QSL at [Formula: see text] and a second transition at a higher field [Formula: see text] to a gapped partially polarized phase, where K is the stre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel ND,Trivedi N

更新日期：2019-06-18 00:00:00

abstract：:In flowering plants, the asymmetrical division of the zygote is the first hallmark of apical-basal polarity of the embryo and is controlled by a MAP kinase pathway that includes the MAPKKK YODA (YDA). In Arabidopsis, YDA is activated by the membrane-associated pseudokinase SHORT SUSPENSOR (SSP) through an unusual pare...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neu A,Eilbert E,Asseck LY,Slane D,Henschen A,Wang K,Bürgel P,Hildebrandt M,Musielak TJ,Kolb M,Lukowitz W,Grefen C,Bayer M

更新日期：2019-03-19 00:00:00

abstract：:IFNs are a family of cytokines that are essential for the antiviral response in vertebrates. Not surprisingly, viruses have adapted to encode virulence factors to cope with the IFN response. Intriguingly, we show here that all three types of interferons, IFN-α, IFN-γ, and IFN-λ, efficiently promote infection by a huma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao X,Guo F,Liu F,Cuconati A,Chang J,Block TM,Guo JT

更新日期：2014-05-06 00:00:00

abstract：:Many empirical studies throughout the social and biomedical sciences focus only on very narrow outcomes such as income, or a single specific disease state, or a measure of positive affect. Human well-being or flourishing, however, consists in a much broader range of states and outcomes, certainly including mental and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： VanderWeele TJ

更新日期：2017-08-01 00:00:00

abstract：:Microcin B17 (MccB17) is a 3.1-kDa Escherichia coli antibiotic that contains thiazole and oxazole heterocycles in a peptide backbone. MccB17 inhibits its cellular target, DNA gyrase, by trapping the enzyme in a complex that is covalently bound to double-strand cleaved DNA, in a manner similar to the well-known quinolo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zamble DB,Miller DA,Heddle JG,Maxwell A,Walsh CT,Hollfelder F

更新日期：2001-07-03 00:00:00

abstract：:The post-translational proteolytic conversion of somatostatin-14 precursors was studied to characterize the enzyme system responsible for the production of the tetradecapeptide either from its 15-kDa precursor protein or from its COOH-terminal fragment, somatostatin-28. A synthetic undecapeptide Pro-Arg-Glu-Arg-Lys-Al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gluschankof P,Morel A,Gomez S,Nicolas P,Fahy C,Cohen P

更新日期：1984-11-01 00:00:00

abstract：:The ribosomal RNA genes (rDNA) of Tetrahymena thermophila macronucleus exist as free linear 21-kilobase molecules that contain replication origins and telomeres. A mutation in this gene confers resistance to the antibiotic paromomycin. We have isolated rDNA from such a mutant (strain p2f), microinjected it into the ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tondravi MM,Yao MC

更新日期：1986-06-01 00:00:00

abstract：:We construct a class of global exact solutions of the Einstein equations that extend the Oppeheimer-Snyder model to the case of nonzero pressure, inside the black hole, by incorporating a shock wave at the leading edge of the expansion of the galaxies, arbitrarily far beyond the Hubble length in the Friedmann-Robertso...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smoller J,Temple B

更新日期：2003-09-30 00:00:00

abstract：:An investigation by paramagnetic resonance spectroscopy of the photoreduction of ferredoxin, oxygenically by water and anoxygenically by a direct electron donor to photosystem I, led to the unexpected findings that different reductive mechanisms may be involved. Ferredoxin photoreduced by water was not reoxidized in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arnon DI,Tsujimoto HY,Tang GM

更新日期：1980-05-01 00:00:00

abstract：:We study the electrophoretic transport of single-stranded RNA molecules through 1.5-nm-wide pores of carbon nanotube membranes by molecular dynamics simulations. From approximately 170 individual RNA translocation events analyzed at full atomic resolution of solvent, membrane, and RNA, we identify key factors in membr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeh IC,Hummer G

更新日期：2004-08-17 00:00:00

abstract：:Transcription initiation is a dynamic process in which RNA polymerase (RNAP) and promoter DNA act as partners, changing in response to one another, to produce a polymerase/promoter open complex (RPo) competent for transcription. In Escherichia coli RNAP, region 1.1, the N-terminal 100 residues of sigma(70), is thought...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hook-Barnard IG,Hinton DM

更新日期：2009-01-20 00:00:00