Low-intensity pulsed ultrasound promotes tissue regeneration in rat dental follicle cells in a porous ceramic scaffold.

Abstract:

:The aim of this study was to investigate the effects of low-intensity pulsed ultrasound (LIPUS) on the osteogenic differentiation of dental follicle cells (DFCs) in vitro and on the regenerative effects of DFC-OsteoBoneTM complexes in vivo. DFCs were isolated and characterized. In the in vitro study, DFCs were cultured in an osteogenic medium in the presence or absence of LIPUS. The expression levels of ALP, Runx2, OSX, and COL-I mRNA were analyzed using real-time polymerase chain reaction (RT-PCR) on day 7. Alizarin red staining was performed on day 21. The state of the growth of the DFCs that were seeded on the scaffold at 3, 5, 7, and 9 days was detected by using a scanning electron microscope. In our in vivo study, 9 healthy nude mice randomly underwent subcutaneous transplantation surgery in one of three groups: group A, empty scaffold; group B, DFCs + scaffold; and group C, DFCs + scaffold + LIPUS. After 8 weeks of implantation, a histological analysis was performed by HE and Mason staining. Our results indicate that LIPUS promotes the osteogenic differentiation of DFCs by increasing the expression of the ALP, Runx2, OSX, and COL-I genes and the formation of mineralized nodules. The cells can adhere and grow on the scaffolds and grow best at 9 days. The HE and Mason staining results showed that more cells, fibrous tissue and blood vessels could be observed in the DFCs + scaffold + LIPUS group than in the other groups. LIPUS could promote the osteogenic differentiation of DFCs in vitro and promote tissue regeneration in a DFCs-scaffold complex in vivo. Further studies should be conducted to explore the underlying mechanisms of LIPUS.

journal_name

Braz Oral Res

journal_title

Brazilian oral research

authors

Kuang Y,Hu B,Xia Y,Jiang D,Huang H,Song J

doi

10.1590/1807-3107bor-2019.vol33.0045

subject

Has Abstract

pub_date

2019-09-09 00:00:00

pages

e0045

eissn

1806-8324

issn

1807-3107

pii

S1806-83242019000100265

journal_volume

33

pub_type

杂志文章
  • Effect of filling technique on the bond strength of methacrylate and silorane-based composite restorations.

    abstract::The bond strength of methacrylate (Z350, 3M ESPE) and silorane (P90, 3M ESPE) restorations, using different cavity filling techniques, was investigated. Cavities (6 × 3 × 3) in bovine teeth were filled using bulk, oblique, or horizontal increments. A push-out test was carried out after 24 h. Data were statistically an...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2016.vol30.0044

    authors: Machado FW,Borges FB,Cenci MS,Moraes RR,Boscato N

    更新日期:2016-01-01 00:00:00

  • Reclassification and treatment of odontogenic keratocysts: A cohort study.

    abstract::The odontogenic keratocyst (OKC) is a recurrent cyst that has been recently reclassified from an odontogenic tumor to an odontogenic cyst. The aim of the present study was to investigate its treatment and address issues related to its association with nevoid basal cell carcinoma syndrome (NBCCS). Lesions from the coho...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2017.vol31.0098

    authors: Ribeiro-Júnior O,Borba AM,Alves CAF,Gouveia MM,Deboni MCZ,Naclério-Homem MDG

    更新日期:2017-12-18 00:00:00

  • Risk factors associated with the failure of miniscrews - A ten-year cross sectional study.

    abstract::The aims of this study were to identify (1) patient-related factors (sex, age, craniofacial pattern and smoking habit), (2) miniscrews implants (MSI)-related factors (length and diameter) and (3) location-related factors [bone (maxilla or mandible) and area (buccal, lingual and alveolar ridge)] that may be associated ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2016.vol30.0124

    authors: Melo AC,Andrighetto AR,Hirt SD,Bongiolo AL,Silva SU,Silva MA

    更新日期:2016-10-24 00:00:00

  • Assessment of the flexural strength of two heat-curing acrylic resins for artificial eyes.

    abstract::Prosthetic eyes are artificial substitutes for the eyeball, made of heat-curing acrylic resin, serving to improve the esthetic appearance of the mutilated patient and his/her inclusion in society. The aim of this study was to assess the flexural strength of two heat-curing acrylic resins used for manufacturing prosthe...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242009000300007

    authors: Fernandes AU,Portugal A,Veloso LR,Goiato MC,Santos DM

    更新日期:2009-07-01 00:00:00

  • Effect of different factors on patient perception of surgical discomfort in third molar surgery.

    abstract::The aim of this study was to evaluate patient perception of surgical discomfort in third molar surgery and the association with clinical variables and polymorphisms associated with the FKBP5, SLC6A4, and COMT genes. This cross-sectional observational study was carried out on 196 participants aged between 18 and 64 yea...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2021.vol35.0007

    authors: Reis GEDS,Calixto RD,Petinati MFP,Souza JF,Kuchler EC,Costa DJD,Bonotto D,Rebellato NLB,Scariot R

    更新日期:2020-11-13 00:00:00

  • Epidemiologic survey of traumatic dental injuries in children seen at the Federal University of Rio de Janeiro, Brazil.

    abstract::This epidemiologic survey aimed at assessing the prevalence of traumatic dental injuries in children seen at the Federal University of Rio de Janeiro, Brazil. The records of a total of 111 children (aged 0 to 6 years) seen from 2004 to 2006 in the dental trauma clinic were surveyed, comprising a total of 201 traumatiz...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242010000100015

    authors: Jesus MA,Antunes LA,Risso Pde A,Freire MV,Maia LC

    更新日期:2010-01-01 00:00:00

  • Psychological factors and the incidence of temporomandibular disorders in early adolescence.

    abstract::The purpose of the present study was to evaluate the relationship between psychological variables and the clinical diagnosis of temporomandbular disorders (TMD) in 12-year-old adolescents. TMD pain was assessed by RDC/TMD examination (Research Diagnostic Criteria for Temporomandibular Disorders) (Axis I and II). Five-...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242009000200011

    authors: Pereira LJ,Pereira-Cenci T,Pereira SM,Cury AA,Ambrosano GM,Pereira AC,Gavião MB

    更新日期:2009-04-01 00:00:00

  • Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women.

    abstract::The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242012000500011

    authors: Machado FC,Cesar DE,Assis AV,Diniz CG,Ribeiro RA

    更新日期:2012-09-01 00:00:00

  • Evaluation of the dental structure loss produced during maintenance and replacement of occlusal amalgam restorations.

    abstract::The aim of this in vitro study was to evaluate four different approaches to the decision of changing or not defective amalgam restorations in first primary molar teeth concerning the loss of dental structure. Ditched amalgam restorations (n = 11) were submitted to four different treatments, as follows: Control group -...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242008000300009

    authors: Sardenberg F,Bonifácio CC,Braga MM,Imparato JC,Mendes FM

    更新日期:2008-07-01 00:00:00

  • HPV in oral squamous cell carcinomas of a Brazilian population: amplification by PCR.

    abstract::Human Papilomaviruses (HPV) are a group of viruses associated with benign and malignant lesions of cutaneous and mucosal epithelia. Some "high risk" HPV types, especially HPV 16 and 18, are strongly correlated with cervical and anogenital cancers and are also related to the genesis of oral squamous cell carcinomas (OS...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242006000100005

    authors: Rivero ER,Nunes FD

    更新日期:2006-01-01 00:00:00

  • Effect of root canal filling techniques on the bond strength of epoxy resin-based sealers.

    abstract::The aim of this study was to evaluate the effects of different root canal filling techniques on the bond strength of epoxy resin-based sealers. Sixty single-rooted canines were prepared using ProTaper (F5) and divided into the following groups based on the root filling technique: Lateral Compaction (LC), Single Cone (...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2016.vol30.0024

    authors: Rached-Júnior FJ,Souza AM,Macedo LM,Raucci-Neto W,Baratto-Filho F,Silva BM,Silva-Sousa YT

    更新日期:2016-01-01 00:00:00

  • Importance of periodontal ligament thickness.

    abstract::This study evaluated whether periodontal ligament (PL) thickness varied with root size and examined the possible influence of this variation on orthodontic mechanics. Measurements were taken of the maxillary left first molar in 54 male Wistar rats. Mean mesial and distal PL thicknesses were compared between the interm...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242013000100014

    authors: Cuoghi OA,Tondelli PM,Aiello CA,Mendonça MR,Costa SC

    更新日期:2013-01-01 00:00:00

  • Measurement of oral health quality of life among patients who underwent haematopoietic stem-cell transplantation.

    abstract::Oral mucositis is a painful condition that occurs in patients who have undergone haematopoietic stem-cell transplantation (HSCT) and has a huge impact on their quality of life. The objective of this study was to examine the extent to which interleukin-6 (IL-6) and tumour necrosis factor alpha (TNF-α) are associated wi...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107BOR-2018.vol32.0078

    authors: Pereira NF,Silva PVRD,Fukuoka CY,Michel-Crosato E,Gonçalves AS,Alves FA,Vieira GMM,Biazevic MGH

    更新日期:2018-07-23 00:00:00

  • Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans.

    abstract::Candida infection is an important cause of morbidity and mortality in immunocompromised patients. The increase in its incidence has been associated with resistance to antimicrobial therapy and biofilm formation. The aim of this study was to evaluate the efficacy of tea tree oil (TTO) and its main component - terpinen-...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2020.vol34.0050

    authors: Francisconi RS,Huacho PMM,Tonon CC,Bordini EAF,Correia MF,Sardi JCO,Spolidorio DMP

    更新日期:2020-06-05 00:00:00

  • Radiopacity of endodontic materials using two models for conversion to millimeters of aluminum.

    abstract::The aims of the present study were to compare conventional radiography, radiographs digitized with a scanner or photographic camera, and digital radiography, used to evaluate the radiopacity of endodontic materials, and to compare the accuracy of linear and quadratic models used to convert radiopacity values to equiva...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2020.vol34.0080

    authors: Ochoa-RodrÍguez VM,Wilches-Visbal JH,Roma B,Coaguila-Llerena H,Tanomaru-Filho M,GonÇalves A,Spin-Neto R,Faria G

    更新日期:2020-01-01 00:00:00

  • Analysis of 153 cases of odontogenic cysts in a South Indian sample population: a retrospective study over a decade.

    abstract::The purpose of this study was to determine the prevalence of odontogenic cysts and to identify their clinico-pathological features among patients by studying biopsy specimens obtained from the archives of the Department of Oral and Maxillofacial Pathology, College of Dental Sciences, Davangere, Karnataka, India, durin...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242012005000007

    authors: Selvamani M,Donoghue M,Basandi PS

    更新日期:2012-07-01 00:00:00

  • Use of chewing gum containing 15% of xylitol and reduction in mutans streptococci salivary levels.

    abstract::Frequent use of Xylitol may decrease the S. mutans levels. However, very little is known about whether this effect on the levels of cariogenic bacteria is maintained after the interruption of short-term usage of xylitol. This study aimed at evaluating changes in mutans streptococci (MS) salivary levels after using a c...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242010000200003

    authors: Fraga CP,Mayer MP,Rodrigues CR

    更新日期:2010-04-01 00:00:00

  • Effects of different concentrations of carbamide peroxide and bleaching periods on the roughness of dental ceramics.

    abstract::The wide use of dental bleaching treatment has brought concern about the possible effects of hydrogen peroxide on dental tissue and restorative materials. The objective of this study was to evaluate in vitro the effect of nightguard bleaching on the surface roughness of dental ceramics after different periods of bleac...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242011000500013

    authors: Ourique SA,Arrais CA,Cassoni A,Ota-Tsuzuki C,Rodrigues JA

    更新日期:2011-09-01 00:00:00

  • Clinical and biochemical evaluation of the saliva of patients with xerostomia induced by radiotherapy.

    abstract::Clinical aspects and biochemical properties in the saliva of 21 patients prior to and following radiotherapy for head and neck cancer were evaluated (experimental group) and compared with the same properties in a control group of 21 subjects free of cancer. Salivary flow was evaluated by measuring the time necessary, ...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242004000100013

    authors: de Barros Pontes C,Polizello AC,Spadaro AC

    更新日期:2004-01-01 00:00:00

  • Comparison among dental, skeletal and chronological development in HIV-positive children: a radiographic study.

    abstract::The goal of this study was to evaluate skeletal, dental and chronological development in an HIV-positive group of children, as compared with a control group, during a four-year period. Panoramic radiographs and hand and wrist radiographs of 60 children were taken. The children, of both sexes, aged 5 years and 2 months...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242005000300010

    authors: Holderbaum RM,Veeck EB,Oliveira HW,Silva CL,Fernandes A

    更新日期:2005-07-01 00:00:00

  • Torsional fatigue strength of reciprocating and rotary pathfinding instruments manufactured from different NiTi alloys.

    abstract::To evaluate the torsional properties of engine-driven pathfinding instruments manufactured from different NiTi alloys - R-Pilot (tip size 12.5;.04 taper; M-Wire) and One G (tip size 14;.03 taper; Conventional NiTi). A total of 40 NiTi instruments from engine-driven pathfinding instruments (n = 20) were used. The torsi...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2019.vol33.0097

    authors: Vivan RR,Alcalde MP,Candeiro G,Gavini G,Caldeira CL,Duarte MAH

    更新日期:2019-10-28 00:00:00

  • Etiologic role of root canal infection in apical periodontitis and its relationship with clinical symptomatology.

    abstract::Evidence shows the polymicrobial etiology of endodontic infections, in which bacteria and their products are the main agents for the development, progression, and dissemination of apical periodontitis. Microbial factors in necrotic root canals (e.g., endotoxin) may spread into apical tissue, evoking and supporting a c...

    journal_title:Brazilian oral research

    pub_type: 杂志文章,评审

    doi:10.1590/1807-3107bor-2018.vol32.0069

    authors: Gomes BPFA,Herrera DR

    更新日期:2018-10-18 00:00:00

  • Effect of the sense of coherence and associated factors in the quality of life of head and neck cancer patients.

    abstract::The sense of coherence (SOC) is a measure of global orientation regarding the ability of individuals to cope with stressful situations. The aim of the present study was to evaluate the association between SOC and quality of life (QoL) and clinical and sociodemographic characteristics among survivors of oral, oropharyn...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2020.vol34.0009

    authors: Bonzanini LIL,Soldera EB,Ortigara GB,Schulz RE,Knorst JK,Ardenghi TM,Ferrazzo KL

    更新日期:2020-02-07 00:00:00

  • Periodontal conditions of Colombian university students aged 16 to 35.

    abstract::Epidemiological data on the periodontal health and oral hygiene practices of young individuals in Latin America are limited. This study was conducted in a sample of 355 first-year students randomly selected from 3,251 new students entering the largest university in Colombia. Participants received a periodontal examina...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2014.vol28.0009

    authors: Marulanda AM,Coral D,Sabogal D,Serrano C

    更新日期:2014-01-01 00:00:00

  • Mouthwashes for the control of supragingival biofilm and gingivitis in orthodontic patients: evidence-based recommendations for clinicians.

    abstract::Properly performed daily mechanical biofilm control is the most important prevention strategy for periodontal diseases. However, proper mechanical biofilm control is not performed effectively by the majority of the population, mainly due to lack of motivation and of manual dexterity. Local biofilm retention factors ma...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2014.vol28.0021

    authors: Haas AN,Pannuti CM,Andrade AK,Escobar EC,Almeida ER,Costa FO,Cortelli JR,Cortelli SC,Rode SD,Pedrazzi V,Oppermann RV

    更新日期:2014-07-11 00:00:00

  • Influence of chlorhexidine application on longitudinal adhesive bond strength in deciduous teeth.

    abstract::The aim of this study was to evaluate the influence of applying 2% chlorhexidine for 30 seconds after phosphoric acid conditioning of dentin on the immediate and long-term bond strengths in deciduous teeth. The occlusal enamel was removed from 40 human sound deciduous molars, which were exfoliated by natural means, an...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242011000500003

    authors: Leitune VC,Portella FF,Bohn PV,Collares FM,Samuel SM

    更新日期:2011-09-01 00:00:00

  • Effect of light curing tip distance and resin shade on microhardness of a hybrid resin composite.

    abstract::Resin composite shades and resin composite polymerization performed with a distanced light tip are factors that can affect polymerization effectiveness. This in vitro study aimed to evaluate the influence of curing tip distance and resin shade on the microhardness of a hybrid resin composite (Z250-3M ESPE). Forty-five...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/s1806-83242005000400012

    authors: Aguiar FH,Lazzari CR,Lima DA,Ambrosano GM,Lovadino JR

    更新日期:2005-10-01 00:00:00

  • Assessment of apically extruded debris and irrigant produced by different nickel-titanium instrument systems.

    abstract::The aim of this study was to evaluate the preparation time and the amounts of apically extruded debris and irrigant using different nickel-titanium instrumentation systems. Forty-five extracted single-rooted mandibular premolar teeth were selected and divided into three groups. The root canals were instrumented accord...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2015.vol29.0002

    authors: Küçükyilmaz E,Savas S,Saygili G,Uysal B

    更新日期:2015-01-01 00:00:00

  • Can heat-pressed feldspathic ceramic be submitted to multiple heat-pressing?

    abstract::The aim of this study was to determine if multiple processing (heat-pressing) of a dental ceramic influences flexural strength, hardness, and microstructure. Ninety bar-shaped specimens (15 mm × 4 mm × 2 mm) of a pressed ceramic (Vita PM9) were fabricated and randomly divided into 6 groups (n = 15) according to the fa...

    journal_title:Brazilian oral research

    pub_type: 杂志文章

    doi:10.1590/1807-3107bor-2018.vol32.0106

    authors: Dal Piva AMO,Barcellos ASP,Bottino MA,Souza ROAE,Melo RM

    更新日期:2018-10-25 00:00:00

  • Is there justification for prophylactic extraction of third molars? A systematic review.

    abstract::The present systematic review was performed to investigate if there is evidence justifying the prophylactic extraction of third molars, one of the most frequent procedures in oral surgery. A series of searches was carried out for randomized, clinical trials and systematic reviews in seven databases (MEDLINE, BBO, LILA...

    journal_title:Brazilian oral research

    pub_type: 杂志文章,评审

    doi:

    authors: Costa MG,Pazzini CA,Pantuzo MC,Jorge ML,Marques LS

    更新日期:2013-03-01 00:00:00