Antibiofilm efficacy of tea tree oil and of its main component terpinen-4-ol against Candida albicans.

Abstract:

:Candida infection is an important cause of morbidity and mortality in immunocompromised patients. The increase in its incidence has been associated with resistance to antimicrobial therapy and biofilm formation. The aim of this study was to evaluate the efficacy of tea tree oil (TTO) and its main component - terpinen-4-ol - against resistant Candida albicans strains (genotypes A and B) identified by molecular typing and against C. albicans ATCC 90028 and SC 5314 reference strains in planktonic and biofilm cultures. The minimum inhibitory concentration, minimum fungicidal concentration, and rate of biofilm development were used to evaluate antifungal activity. Results were obtained from analysis of the biofilm using the cell proliferation assay 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) and confocal laser scanning microscopy (CLSM). Terpinen-4-ol and TTO inhibited C. albicans growth. CLSM confirmed that 17.92 mg/mL of TTO and 8.86 mg/mL of terpinen-4-ol applied for 60 s (rinse simulation) interfered with biofilm formation. Hence, this in vitro study revealed that natural substances such as TTO and terpinen-4-ol present promising results for the treatment of oral candidiasis.

journal_name

Braz Oral Res

journal_title

Brazilian oral research

authors

Francisconi RS,Huacho PMM,Tonon CC,Bordini EAF,Correia MF,Sardi JCO,Spolidorio DMP

doi

10.1590/1807-3107bor-2020.vol34.0050

subject

Has Abstract

pub_date

2020-06-05 00:00:00

pages

e050

eissn

1806-8324

issn

1807-3107

pii

S1806-83242020000100236

journal_volume

34

pub_type

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