HPV in oral squamous cell carcinomas of a Brazilian population: amplification by PCR.

Abstract:

:Human Papilomaviruses (HPV) are a group of viruses associated with benign and malignant lesions of cutaneous and mucosal epithelia. Some "high risk" HPV types, especially HPV 16 and 18, are strongly correlated with cervical and anogenital cancers and are also related to the genesis of oral squamous cell carcinomas (OSCC). The aim of this work was to investigate the incidence of HPV infection in 40 paraffin-embedded or fresh specimens of OSCC, using PCR amplification of the viral DNA. Literature based primers (GP5+/GP6+) were used in order to amplify HPV DNA from the L1 gene, present in more than 22 types of HPV. A condyloma case with HPV 16 and 18 detected by in situ hybridization was used as a positive control. Amplification of HPV was observed only in the positive control. No squamous cell carcinoma cases showed DNA viral amplification. Absence of HPV DNA amplification by PCR in the analyzed specimens of OSCCs suggests that this virus not always plays a role in the carcinogenesis process. Discrepancy with some studies found in the literature may be related to methodology or population differences.

journal_name

Braz Oral Res

journal_title

Brazilian oral research

authors

Rivero ER,Nunes FD

doi

10.1590/s1806-83242006000100005

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

21-4

issue

1

eissn

1806-8324

issn

1807-3107

pii

S1806-83242006000100005

journal_volume

20

pub_type

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