Comparison of HRM analysis and three REP-PCR genomic fingerprint methods for rapid typing of MRSA at a Brazilian hospital.

Abstract:

INTRODUCTION:Infections caused by multidrug-resistant bacteria are increasingly common and represent a serious problem for public health. Staphylococcus aureus is one of the major agents of infections, and methicillin-resistant S. aureus (MRSA) has spread worldwide. The aim of this study was to phenotypically and genotypically characterize 55 MRSAs isolated in the University Hospital of Londrina, Paraná, Brazil, during 2010. METHODOLOGY:Bacterial isolates were characterized based on their antimicrobial susceptibility profile, biofilm production capacity, and staphylococcal chromosome cassette mec (SCCmec) type. Determination of clonal groups was performed by polymerase chain reaction using the RW3A, JB1, and BOX A1R primers and high-resolution melting (HRM) analysis. RESULTS:The majority of isolates harbored SCCmec type II. SCCmec III, characteristic of the Brazilian endemic clone, was observed in four strains. Only two isolates harbored SCCmec type IV, which is common in community-acquired MRSA strains. Most isolates also showed resistance to more than four of the tested antimicrobials, and 30 isolates exhibited the ability to produce biofilm. DNA polymorphism analysis showed a higher discriminatory power for the JB1 primer, but RW3A revealed several clonal groups of MRSA with similar genotypic and phenotypic characteristics. HRM analysis showed eight different sequence types. CONCLUSIONS:These results are important for epidemiological studies involving MRSA infections.

journal_name

J Infect Dev Ctries

authors

Bodnar GC,Martins HM,De Oliveira CF,Morey AT,Tavares ER,Cardoso JD,Perugini MR,Lioni LM,Yamada-Ogatta SF,Kobayashi RK,Nakazato G

doi

10.3855/jidc.7887

subject

Has Abstract

pub_date

2016-12-30 00:00:00

pages

1306-1317

issue

12

eissn

2036-6590

issn

1972-2680

journal_volume

10

pub_type

杂志文章
  • Ascaris lumbricoides infection and parasite load are associated with asthma in children.

    abstract:INTRODUCTION:Association between Ascaris lumbricoides infection and asthma is a controversial subject that has been studied by several authors based on the hygiene theory. This work contributes to better understanding this issue. METHODOLOGY:This was a cross-sectional study involving 1,004 children from a neighborhood...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.3585

    authors: Bragagnoli G,Silva MT

    更新日期:2014-07-14 00:00:00

  • Prevalence and behavioral risk factors for STIs/HIV among attendees of the Ministry of Health hospitals in Saudi Arabia.

    abstract:INTRODUCTION:Sexually transmitted infections (STI) are a major public health, social, and economic problem leading to morbidity, mortality, and stigma. This study was conducted to determine the prevalence of STIs, investigate behavioral risk factors and the relationship between the STIs/HIV and demographic factors. ME...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.5964

    authors: Filemban SM,Yasein YA,Abdalla MH,Al-Hakeem R,Al-Tawfiq JA,Memish ZA

    更新日期:2015-04-15 00:00:00

  • Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan.

    abstract:INTRODUCTION:The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of H. pylori genotypes ...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.2942

    authors: Khan A,Farooqui A,Raza Y,Rasheed F,Manzoor H,Akhtar SS,Quraishy MS,Rubino S,Kazmi SU,Paglietti B

    更新日期:2013-03-14 00:00:00

  • Unusual mode of transmission of dengue.

    abstract::Dengue is the most important tropical mosquito-borne infectious disease caused by an arbovirus, the dengue virus. It should be noted that there are still other unusual modes of transmission of dengue infection. This paper summarizes those non vector-borne transmissions of dengue including vertical transmission, transf...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章,评审

    doi:10.3855/jidc.145

    authors: Wiwanitkit V

    更新日期:2009-11-30 00:00:00

  • Malaria knowledge and treatment adherence in a Brazilian Amazon community.

    abstract:INTRODUCTION:Malaria remains an important public health problem despite recent scientific breakthroughs regarding knowledge about malaria and treatment strategies. The objective of this study was to analyze malaria patients' knowledge about the disease, its treatment and prevention, linking it to drug treatment adheren...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.7129

    authors: Souza TG,Reiners AA,Azevedo RC,Fontes CJ,Ferreira RG,Do Carmo PU

    更新日期:2016-11-24 00:00:00

  • Plasmodium falciparum and Schistosoma mansoni coinfection and the side benefit of artemether-lumefantrine in malaria patients.

    abstract:INTRODUCTION:The distribution of both malaria and schistosomiasis exhibits a large geographical overlap in tropical environments, particularly in sub-Saharan Africa. This part of the world currently harbours more than 85% of the estimated global burden of these diseases. Studies showed that artemisinin derivatives used...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.2658

    authors: Abay SM,Tilahun M,Fikrie N,Habtewold A

    更新日期:2013-06-15 00:00:00

  • The Human papillomavirus among women living with Human Immunodeficiency Virus in Morocco: A prospective cross-sectional study.

    abstract:INTRODUCTION:Women infected with human immunodeficiency virus (HIV) have a higher risk of contracting human papillomavirus (HPV) infections and are more prone to develop cervical cancer. The objective of this study was to determine the prevalence of HPV and its association with risk factors among Moroccan women living ...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.9711

    authors: Ouladlahsen A,Fayssel N,Bensghir R,Baba H,Lamdini H,Sodqi M,Marih L,Essebbani M,Nadifi S,Benjelloun S,Himmich H,Chakib A,Wakrim L,Marhoum El Filali K,Ezzikouri S

    更新日期:2018-06-30 00:00:00

  • COVID-19, indications for professional football teams and referees training resumption.

    abstract::These indications were drawn up by the Federal Medical-Scientific Commission (FIGC Commission), supplemented for the necessary time by some experts on the subject; currently they are intended to grant the highest achievable guarantee level to protect the health of players, referees and all professionals involved in ca...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.13436

    authors: Donadu MG,Pizzi AR,Zeppilli P

    更新日期:2020-10-31 00:00:00

  • Knowledge, attitudes, and practices about tuberculosis and choice of communication channels in Thailand.

    abstract:INTRODUCTION:The aim of this study was to assess tuberculosis (TB) knowledge, attitudes, and practices in both the general population and risk groups in Thailand. METHODOLOGY:In a cross-sectional survey, a general population (n = 3,074) and family members of a TB patient (n = 559) were randomly selected, using stratif...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.6963

    authors: Pengpid S,Peltzer K,Puckpinyo A,Tiraphat S,Viripiromgool S,Apidechkul T,Sathirapanya C,Leethongdee S,Chompikul J,Mongkolchati A

    更新日期:2016-08-02 00:00:00

  • Medical-surgical management and clinical outcome in cervical abscesses.

    abstract:INTRODUCTION:This study aims at defining through a retrospective evaluation, the clinical parameters affecting the clinical course and consequently the management of patients presenting with cervicofacial abscesses. METHODOLOGY:A total of 394 patients diagnosed with abscess at the University of Sassari Otorhinolaryngo...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.12191

    authors: Melis A,Riu F,Kihlgren C,Piras A,Rizzo D,Sotgiu A,Donadu M,Usai D,Zanetti S,Bussu F

    更新日期:2020-05-31 00:00:00

  • Clinical report of serious complications associated with measles pneumonia in children hospitalized at Shengjing hospital, China.

    abstract:INTRODUCTION:Despite tremendous progress made toward elimination, measles continues to pose a great threat to the health of children in developing countries. The objective of this study was to summarize and analyze the clinical characteristics and treatment experience of serious complications of measles pneumonia in ch...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.6534

    authors: Li J,Zhao Y,Liu Z,Zhang T,Liu C,Liu X

    更新日期:2015-10-29 00:00:00

  • Antibiotic resistance and detection of the sul2 gene in urinary isolates of Escherichia coli in patients from Brazil.

    abstract:INTRODUCTION:The present study aimed to assess the antibiotic resistance profiles and detect the presence of the sul2 gene in sulfamethoxazole-susceptible and resistant isolates of Escherichia coli obtained from outpatients and inpatients with urinary tract infections. METHODOLOGY:The resistance profiles of 739 strain...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.3380

    authors: Teichmann A,Agra HN,Nunes Lde S,da Rocha MP,Renner JD,Possuelo LG,Carneiro M,Rieger A,Benitez LB,Valim AR

    更新日期:2014-01-15 00:00:00

  • Brucella suis bacteremia misidentified as Ochrobactrum anthropi by the VITEK 2 system.

    abstract::Ochrobactrum and Brucella are genetically related genera of the family Brucellaceae, sharing 98.8% rRNA similarity. Because of their phenotypic similarity, Ochrobactrum can be miscoded as Brucella by automated identification systems. The misidentification on blood cultures (BCs) of B. suis as O. anthropi by the VITEK ...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.7532

    authors: Vila A,Pagella H,Vera Bello G,Vicente A

    更新日期:2016-04-28 00:00:00

  • Seroprevalence of human cysticercosis and its associated risk factors among humans in areas of Kaduna metropolis, Nigeria.

    abstract:INTRODUCTION:Taenia solium cysticercosis is considered an emerging parasitic zoonosis of global importance due to its impact on both agriculture and public health in developing countries. Epidemiological information on human cysticercosis is limited in Nigeria. This study was conducted to determine the seroprevalence o...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.5415

    authors: Edia-Asuke AU,Inabo HI,Mukaratirwa S,Umoh VJ,Whong CM,Asuke S,Ella EE

    更新日期:2015-08-29 00:00:00

  • Gallbladder microbiota variability in Colombian gallstones patients.

    abstract:INTRODUCTION:Gallbladder stones are a very frequently occurring condition. Despite bile bactericidal activity, many bacteria have been detected inside the gallbladder, and gallstones facilitate their presence. Between 3% and 5% of the patients with Salmonella spp. infection develop the carrier stage, with the bacteria ...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.8258

    authors: Arteta AA,Carvajal-Restrepo H,Sánchez-Jiménez MM,Diaz-Rodriguez S,Cardona-Castro N

    更新日期:2017-03-31 00:00:00

  • Ribavirin therapy for Chikungunya arthritis.

    abstract:BACKGROUND:Chikungunya is an acute viral infection presenting with a febrile episode and severe arthralgia, swelling of soft tissues, especially around the ankles. Many patients recover with nonspecific treatment of analgesics. Some patients continue to have subacute crippling arthritis in the legs affecting their mobi...

    journal_title:Journal of infection in developing countries

    pub_type: 临床试验,杂志文章

    doi:

    authors: Ravichandran R,Manian M

    更新日期:2008-04-01 00:00:00

  • Fasciolopsis buski infection in a Vietnamese pregnant woman with systemic lupus erythematosus.

    abstract::A clinical case of infection caused by Fasciolopsis buski in a 24 weeks pregnant woman from Vietnam affected by systemic lupus erythematosus (SLE) is reported here. On 22 February 2012 the patient was admitted to Hue Hospital in Hue, Vietnam, with a diagnosis of general illness and suspected acute anaemia. Laboratory ...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.7017

    authors: Fiamma M,Longoni SS,Ngo TM,Le Phan MT,Santona A,Ton Nu PA,Paglietti B

    更新日期:2015-07-04 00:00:00

  • Identification of Entamoeba histolytica and E. dispar infection in Maceió, Alagoas State, northeast Brazil.

    abstract:INTRODUCTION:A cross-sectional study was carried out to assess the prevalence of E. histolytica and E. dispar by examining stool samples obtained from 1,003 students of public schools in Maceió, Alagoas, Brazil. METHODOLOGY:All stool samples were processed using the spontaneous sedimentation technique and examined mic...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.6781

    authors: Santos RV,Fontes G,Duarte IA,Santos-Júnior JA,Rocha EM

    更新日期:2016-10-31 00:00:00

  • Recognizing true H5N1 infections in humans during confirmed outbreaks.

    abstract:INTRODUCTION:The goal of this study was to evaluate whether any characteristics that are evident at presentation for urgent medical attention could be used to differentiate cases of H5N1 in the absence of viral testing. METHODOLOGY:Information about exposure to poultry, clinical signs and symptoms, treatments, and out...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.3329

    authors: Zaman M,Gasimov V,Oner AF,Dogan N,Adisasmito W,Coker R,Bamgboye EL,Chan PK,Hanshaoworakul W,Lee N,Phommasack B,Touch S,Tsang O,Swenson A,Toovey S,Dreyer NA

    更新日期:2014-02-13 00:00:00

  • Drug resistance patterns of bacteria isolated from patients with nosocomial pneumonia at Tehran hospitals during 2009-2011.

    abstract:INTRODUCTION:Nosocomial pneumonia remains an important cause of mortality and morbidity worldwide. Surveillance programs play an important role in the identification of common etiologic agents and local patterns of antimicrobial resistance. METHODOLOGY:In this study we determined the frequency and antimicrobial suscep...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.2604

    authors: Haeili M,Ghodousi A,Nomanpour B,Omrani M,Feizabadi MM

    更新日期:2013-04-17 00:00:00

  • Potency of detergents in enhancing Schistosoma mansoni tegumental antigens.

    abstract:INTRODUCTION:Vaccine strategies represent an essential component for the future control of schistosomiasis. This work aimed to evaluate the role of detergents for potentiating the effect of Schistosoma mansoni tegumental antigens (TA) against challenge infection. METHODOLOGY:Two detergents; Triton X-100 (TX-100) and s...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.1199

    authors: Hamed MA

    更新日期:2011-03-21 00:00:00

  • Antimicrobial stewardship approach: Prevalence of antimicrobial resistant bacteria at a regional hospital in South Africa.

    abstract:INTRODUCTION:Antimicrobial stewardship practices are crucial for the regular surveillance to change the antimicrobial policy. This study was conducted to decide the prevalence of common bacteria and their antibiogram regarding antimicrobial stewardship program within one year, at the regional and district, Stanger hosp...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.10685

    authors: Han KS,Gustavo L,Rajkumar VC,Swe Swe-Han K

    更新日期:2019-08-31 00:00:00

  • Genotypic and demographic characterization of invasive isolates of Salmonella Typhimurium in HIV co-infected patients in South Africa.

    abstract:BACKGROUND:Non-typhoidal Salmonella are frequently food-borne zoonotic pathogens that may cause invasive disease in HIV-positive individuals. METHODOLOGY:Invasive isolates (n = 652) of Salmonella Typhimurium from human patients in Gauteng Province of South Africa were investigated for the years 2006 and 2007. Bacteria...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.549

    authors: Keddy KH,Dwarika S,Crowther P,Perovic O,Wadula J,Hoosen A,Sooka A,Crewe-Brown HH,Smith AM

    更新日期:2009-09-15 00:00:00

  • Comparative analysis of antibodies and lymphocytes in pulmonary tuberculosis patients with negative and positive cultures.

    abstract:INTRODUCTION:The sputum smear or the culture are the definitive diagnosis of pulmonary tuberculosis. Only a fraction of clinical patients are culture-confirmed. METHODOLOGY:A total of 24 clinical cases (40 ± 14 years old) with positive smear and negative co-morbidity were studied. Cases were selected from 600 patients...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.10583

    authors: Cázares-Sosa FR,Hernández-Solís A,Andrés-Dionicio AE,González-González H,Ibáñez-Hernández M,Cicero-Sabido R,Huerta H,Tapia-Romero R,De-la-Rosa-Arana JL

    更新日期:2019-01-31 00:00:00

  • Giardia duodenalis genotypes among schoolchildren and their families and pets in urban and rural areas of Sinaloa, Mexico.

    abstract:INTRODUCTION:Giardiasis is a human health concern worldwide, especially among schoolchildren. Giardia duodenalis genotypes A and B are infective to humans, but their zoonotic potential remains controversial. In Mexico, the most prevalent genotype is A, but B was also detected in southeastern Mexico. In Sinaloa state, n...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.8223

    authors: García-Cervantes PC,Báez-Flores ME,Delgado-Vargas F,Ponce-Macotela M,Nawa Y,De-la-Cruz-Otero MD,Martínez-Gordillo MN,Díaz-Camacho SP

    更新日期:2017-02-28 00:00:00

  • Antimicrobial resistance in Salmonella enterica serovar Enteritidis in Morocco.

    abstract:INTRODUCTION:Salmonella enterica is recognised worldwide as one of the major agents of human gastrointestinal infections. The aim of the present work is to ascertain the antimicrobial susceptibilities of 150 Salmonella enterica serovar Enteritidis isolates from humans in Morocco during the period from 2000 to 2008. ME...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.806

    authors: Ohmani F,Khedid K,Britel S,Qasmaoui A,Charof R,Filali-Maltouf A,El Aouad R

    更新日期:2010-12-23 00:00:00

  • Bacterial pericarditis and antimicrobial resistance at the Tehran Heart Center, Iran.

    abstract:INTRODUCTION:When bacterial pericarditis is suspected, urgent pericardial drainage combined with intravenous antibacterial therapy is mandatory to avert devastating, life-threatening complications. There have been scanty results on antimicrobial susceptibility of common causative microorganisms of bacterial pericarditi...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.6027

    authors: Sotoudeh Anvari M,Kianinejad R,Boroumand MA,Arzhan S,Jalali A

    更新日期:2015-07-30 00:00:00

  • Critical points and the presence of pathogenic bacteria in iced beverage processing lines.

    abstract:INTRODUCTION:Ice can be contaminated by pathogenic bacteria. This study aimed to identify critical points in iced beverage production and distribution lines to examine the presence of pathogenic bacteria in a beverage and its processing environment, as well as when water and ice used as main ingredients. METHODOLOGY:T...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.8934

    authors: Nababan H,Rahayu WP,Waturangi DE,Suratmono S,Puspitasari R,Indrotristanto N,Nikastri E,Yuliangsih S,Pusparini N

    更新日期:2017-06-30 00:00:00

  • Increasing frequency of Pseudomonas aeruginosa infections during tigecycline use.

    abstract:INTRODUCTION:The widespread use of tigecycline raises the question of increasing infection rates of Pseudomonas aeruginosa (PA) in ICUs which are not affected by this antibiotic. OBJECTIVE:The aim of this study was to determine if treatment with tigecycline is a risk factor for PA infection in ICU patients. METHODOLO...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.4700

    authors: Ulu-Kilic A,Alp E,Altun D,Cevahir F,Kalın G,Demiraslan H

    更新日期:2015-03-15 00:00:00

  • Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients.

    abstract:INTRODUCTION:Intestinal and extraintestinal infections by Aeromonas spp., remain controversial, due to the existence of healthy carriers of Aeromonas spp. In children under five years old, the diarrhea of infectious origin constitutes the second cause of mortality and remains a major concern for public health. The aim ...

    journal_title:Journal of infection in developing countries

    pub_type: 杂志文章

    doi:10.3855/jidc.12966

    authors: Bello-López JM,Sanchez-Garibay C,Ibáñez-Cervantes G,León-García G,Gonzalez-Avila LU,Hernández-Cortez C,Arzate Barbosa P,Castro-Escarpulli G

    更新日期:2020-10-31 00:00:00