Abstract:
INTRODUCTION:Vaccine strategies represent an essential component for the future control of schistosomiasis. This work aimed to evaluate the role of detergents for potentiating the effect of Schistosoma mansoni tegumental antigens (TA) against challenge infection. METHODOLOGY:Two detergents; Triton X-100 (TX-100) and sodium dodecyl sulfate (SDS), were selected for extraction of tegumental proteins. Mice were vaccinated with two doses of each preparation (100 µg protein) at time intervals 15 days before infection. Evaluation was performed by estimating particular metabolic pathways, including the Krebs cycle (via succinate dehydrogenase); glycolysis (lactate dehydrogenase); gluconeogenesis (glucose-6-phosphatase); hydrolytic enzymes (acid phosphatase); and nucleic acid catabolic enzymes (5'-nucleotidase). Serum protein profiles, worm burden, ova counts, spleen and relative liver weights were also investigated. The work was extended to study the histopathological picture of the liver including granuloma count, and total infection area. Vaccinated post-challenged mice showed amelioration of the selected biochemical parameters and reduction in worm and ova count (P ≤ 0.0001) as well as improvement in the histological features of the liver. RESULTS:Triton X-100 potentiated the protective effect of S. mansoni tegumental antigen and extracted effective proteins better than SDS, while TA alone recorded the lowest level of protection. CONCLUSION:TX-100 will be more efficient for future studies in molecular identification of novel candidate tegumental proteins.
journal_name
J Infect Dev Ctriesjournal_title
Journal of infection in developing countriesauthors
Hamed MAdoi
10.3855/jidc.1199subject
Has Abstractpub_date
2011-03-21 00:00:00pages
209-15issue
3eissn
2036-6590issn
1972-2680journal_volume
5pub_type
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.7033
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abstract:INTRODUCTION:Pneumocystis jirovecii (PJ) pneumonia (PJP) is an important opportunistic infection affecting various types of immunocompromised patients and is associated with an increased risk of mortality. PJ is a unique fungal pathogen which is increasingly common and maybe associated with a higher mortality rate in p...
journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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更新日期:2010-03-08 00:00:00
abstract:INTRODUCTION:Salmonella enterica serotype Kentucky was isolated from septic poultry in Nigeria. The objective of this study was to characterize this isolate by screening for SGI1 and hyper-virulence. METHODOLOGY:The strain was characterized by identification of Salmonella genomic island 1 (SGI1) through a PCR assay an...
journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
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abstract::Although malaria is endemic in India, neonatal disease is considered rare. We report a case of neonatal malaria in a 26-day-old neonate with fever and splenomegaly who was diagnosed after a long and unsuccessful battery of tests for splenomegaly. Routine screening for malaria is essential for all neonates with fever i...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
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abstract::Zika virus (ZIKV) is an emerging arbovirus of the Flaviviridae family and is related to dengue, Chikungunya, West Nile, yellow fever, and Japanese encephalitis viruses. ZIKV was first discovered in Uganda in 1947. Different species of mosquito from the Aedes genus, mainly A. aegypti and A. albopictus are the vectors r...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章,评审
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abstract::Orthotopic liver transplantation is a life-saving procedure for patients with end-stage liver failure. However, Acinetobacter baumannii infections and acute rejection are important causes of morbidity and mortality following transplants. Here we present a case report of a cadaveric donor liver transplantation with inf...
journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章,评审
doi:10.3855/jidc.238
更新日期:2009-06-01 00:00:00
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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journal_title:Journal of infection in developing countries
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.5694
更新日期:2015-05-18 00:00:00
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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更新日期:2012-02-13 00:00:00
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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更新日期:2009-10-26 00:00:00
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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更新日期:2016-11-24 00:00:00
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章,随机对照试验
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更新日期:2011-10-13 00:00:00
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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更新日期:2016-10-31 00:00:00
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journal_title:Journal of infection in developing countries
pub_type: 杂志文章
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