Abstract:
INTRODUCTION:The pathogenic bacterium Salmonella enterica serovar Typhimurium elicits a variety of genetic programs to adapt to stress conditions encountered within hostile environments such as host phagocytes and preserved food. METHODOLOGY:In this work, differential display (DD) methodology was used to investigate the effect of one month starvation in a salty microcosm (0.5 M NaCl) on transcript profiling in a Salmonella Typhimurium LT2 strain. cDNA fragments resulting from differentially expressed mRNA were eluted from the gel, re-amplified, cloned, and then sequenced. RESULTS:A total of 21 differentially expressed bands were detected by DD reverse transcription-polymerase chain reaction (RT-PCR). However, only 12 of them were successfully identified as upregulated genes in stressed cells. Based on the sequencing data and BLAST analysis, these genes were sopA, ssaD, yhhK, gmK, cspC, uspA, ompR, phoP, stcC, fimA, acrA,and yehZ. As a confirmation of the differential expression, RT-PCR was carried out using a set of specific primers. Remarkably, the expression levels of these genes were significantly increased in starved bacteria compared to standard laboratory conditions. CONCLUSIONS:Our results indicate that the starvation of Salmonella Typhimurium over one month in a salty microcosm changes the expression of stress proteins, response regulator in a two-component system, outer membrane proteins, effector proteins translocated by Salmonella pathogenicity island SPI1 and SPI2 type III secretion systems (TTSS), several metabolic enzymes, efflux pumps, and transport proteins. This suggests that the expression of the identified genes is important for the response of this pathogen to starvation in salt.
journal_name
J Infect Dev Ctriesjournal_title
Journal of infection in developing countriesauthors
Elabed H,Merghni A,Hamza R,Bakhrouf A,Gaddour Kdoi
10.3855/jidc.7033subject
Has Abstractpub_date
2016-01-31 00:00:00pages
74-81issue
1eissn
2036-6590issn
1972-2680journal_volume
10pub_type
杂志文章abstract:INTRODUCTION:Invasive fungal infection (IFI) is a major cause of morbidity and mortality in allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. A previous history of IFI is not an absolute contraindication for allo-HSCT, particularly in the era of secondary antifungal prophylaxis (SAP). Prompt di...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9961
更新日期:2018-09-30 00:00:00
abstract:INTRODUCTION:When bacterial pericarditis is suspected, urgent pericardial drainage combined with intravenous antibacterial therapy is mandatory to avert devastating, life-threatening complications. There have been scanty results on antimicrobial susceptibility of common causative microorganisms of bacterial pericarditi...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6027
更新日期:2015-07-30 00:00:00
abstract:INTRODUCTION:Melioidosis can have multiple organ involvement which can then mimic other infections. The aim of this study was to determine if there are any factors significantly associated with melioidosis which can inform diagnostic evaluations before receiving the results of confirming laboratory testing. METHODOLOG...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10727
更新日期:2019-02-28 00:00:00
abstract:BACKGROUND:Non-typhoidal Salmonella are frequently food-borne zoonotic pathogens that may cause invasive disease in HIV-positive individuals. METHODOLOGY:Invasive isolates (n = 652) of Salmonella Typhimurium from human patients in Gauteng Province of South Africa were investigated for the years 2006 and 2007. Bacteria...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.549
更新日期:2009-09-15 00:00:00
abstract:INTRODUCTION:The correlation of Chlamydia pneumoniae to coronary artery disease (CAD) in Jordan was investigated in this study. METHODOLOGY:Totals of 361 atherosclerotic patients and 392 apparently healthy controls of both sexes were enrolled. C. pneumoniae-specific IgG antibodies were measured by the microimmunofluor...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.7363
更新日期:2016-05-31 00:00:00
abstract:BACKGROUND:Research on the effects of corticosteroids in bacterial meningitis (BM) yielded conflicting results. While some studies reveal that corticosteroids improve the outcomes in BM treatments, others provide strong evidence that patients do not profit from this treatment. We investigated the factors that may impac...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.51
更新日期:2009-12-14 00:00:00
abstract:INTRODUCTION:The novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the coronavirus disease 2019 (COVID-19). First COVID-19 case was detected in March, 10, 2020 in Turkey and as of May, 18, 2020 148,067 cases have been identified and 4096 citizens have died. Tuberculosis (...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.13152
更新日期:2020-07-31 00:00:00
abstract:INTRODUCTION:Due to the shortcomings in the 1997-World Health Organisation (WHO) dengue case classification (DCC), a revised classification was proposed in 2009. This study was aimed to assess the clinical usefulness of the two classifications during a large dengue epidemic. METHODOLOGY:Clinical data of dengue patient...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.12468
更新日期:2020-07-31 00:00:00
abstract:INTRODUCTION:The emergence and rapid spread of blaIMP and blaVIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options. METHODOLOGY:A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudomonas aeruginosa (61),...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2268
更新日期:2012-11-26 00:00:00
abstract:INTRODUCTION:There is limited knowledge about the associations of Helicobacter pylori (H. pylori) infections in developing countries. This study aimed to determine the current prevalence and associations of H. pylori infection with breastfeeding practices, nutritional status, and recurrent abdominal pain (RAP) in a gro...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.3196
更新日期:2014-04-15 00:00:00
abstract:INTRODUCTION:Micronutrient deficiencies are prevalent in developing countries and may influence vulnerability to diseases particularly malaria and its severity. This study investigated serum vitamin A profile of under-five children with severe malaria (SM) in South-western, Nigeria and to determine its association with...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9572
更新日期:2018-05-31 00:00:00
abstract::Leishmaniasis is a clinically heterogeneous syndrome caused by intracellular protozoan parasites of the genus Leishmania. The clinical spectrum of leishmaniasis encompasses subclinical (not apparent), localized (skin lesion), and disseminated (cutaneous, mucocutaneous, and visceral) infection. This spectrum of manifes...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章,评审
doi:10.3855/jidc.4310
更新日期:2014-08-13 00:00:00
abstract:BACKGROUND:In the year 2005, an epidemic of Japanese encephalitis (JE) occurred in the northern states of India. The present study was planned to reconfirm the circulation of JE in the area and to assess the trend of the disease to slow down the burden of JE. METHODOLOGY:Surveillance was conducted to identify patients...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.470
更新日期:2009-08-30 00:00:00
abstract:INTRODUCTION:Uropathogenic Escherichia coli (UPEC) are the main etiological agent of urinary tract infections (UTIs). Association between different serotypes and UTIs is known, however, some strains are incapable to be serotyped. The aim of this work was to study bthe phenotypical and genotypical characteristics of 113...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.11098
更新日期:2019-06-30 00:00:00
abstract::Lebanon yearly witnesses a high flux of expatriates and workers from Dengue virus (DENV) endemic regions. Multiple cases of Dengue fever have been documented at the American University of Beirut Medical Center (AUBMC) in travellers to endemic regions. Given the presence of the Aedes aegypti mosquito in Lebanon, introd...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9954
更新日期:2018-04-30 00:00:00
abstract:INTRODUCTION:Tuberculosis (TB) continues to be a public health problem globally. The burden is further exacerbated in developing countries like Nigeria, by poor diagnosis, management and treatment, as well as rapid emergence of drug-resistant TB. This study was conducted to evaluate the prevalence of drug-resistant TB,...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.12736
更新日期:2020-09-30 00:00:00
abstract:INTRODUCTION:This study sought to identify the association of antibiotic prescribing and the risk of antibiotic-associated diarrhoea (AAD) in Korle Bu Teaching Hospital (KBTH) in Ghana. METHODOLOGY:Patients from the Male Urology Ward and Treatment Room of the Surgical Department of KBTH were followed up over three mon...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.11259
更新日期:2020-08-31 00:00:00
abstract::The case of a Hickman catheter-related bacteremia caused by Gordonia sputi in a patient with breast cancer is presented. Blood cultures grew a Gram-positive rod, susceptible to several antimicrobials, subsequently identified by 16S rRNA gene sequencing as Gordonia sputi. The infection resolved after successful treatme...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1770
更新日期:2012-02-13 00:00:00
abstract:INTRODUCTION:Antimicrobial stewardship practices are crucial for the regular surveillance to change the antimicrobial policy. This study was conducted to decide the prevalence of common bacteria and their antibiogram regarding antimicrobial stewardship program within one year, at the regional and district, Stanger hosp...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10685
更新日期:2019-08-31 00:00:00
abstract::We present the case of a 42-year-old male patient who applied to the emergency department of our hospital with clinical nephritis, orchitis, acute renal failure without endocarditis, and a low-grade fever. Brucella agglutinin titers were 1:160, Rose Bengal test was positive and Brucella melitensis was isolated from ur...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1442
更新日期:2011-12-13 00:00:00
abstract:BACKGROUND:This study was conducted to examine the frequency of contamination in retail meat available in Karachi, Pakistan. METHODOLOGY:Raw meat samples (250) and surface swabs (90) from meat processing equipment and the surrounding environment were analyzed for microbiological contamination. RESULTS:Out of 340 samp...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:
更新日期:2010-06-30 00:00:00
abstract:INTRODUCTION:Salmonella enterica serotype Kentucky was isolated from septic poultry in Nigeria. The objective of this study was to characterize this isolate by screening for SGI1 and hyper-virulence. METHODOLOGY:The strain was characterized by identification of Salmonella genomic island 1 (SGI1) through a PCR assay an...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1988
更新日期:2012-06-15 00:00:00
abstract:INTRODUCTION:The present study was conducted to evaluate the prevalence of Trichuris spp. and other intestinal parasitic infections in owned cats on St. Kitts. METHODOLOGY:The feces of 41 non-feral cats (23 indoor only; 18 indoor/outdoor) were examined for the presence of Trichuris spp. eggs. RESULTS:Nine (22%) of th...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.5778
更新日期:2015-01-15 00:00:00
abstract:INTRODUCTION:Septicemia is considered as an important cause of life-threating infections. The study was aimed at determining the incidence of septicemia considering different age groups and gender among suspected patients admitted to a tertiary care hospital in Iraq. METHODOLOGY:A total of 168 blood samples were colle...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.13089
更新日期:2020-12-31 00:00:00
abstract:INTRODUCTION:Epidemiological studies have shown the association between risk of developing cervical cancer and the persistence of high-risk papillomavirus types in addition to some co-factors. However, little is known about co-factors associated with human papillomavirus (HPV) infection, especially in developing countr...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1675
更新日期:2012-07-23 00:00:00
abstract:INTRODUCTION:Dengue virus (DENV) infection is currently a major cause of morbidity in the world, and its incidence has mainly increased during the last years in Latin America, including Paraguay. The objective of this study was to compare the clinical and laboratory findings of dengue and assess whether the serotype is...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.11584
更新日期:2019-12-31 00:00:00
abstract:INTRODUCTION:Extended-Spectrum Beta-Lactamases (ESBL) are a common mechanism of bacterial resistance in Enterobacteriaceae. The purpose of this study is to characterize the ESBL genes produced by community-acquired uropathogenic Escherichia coli strains in the Nouna District, in the West-African country, Burkina Faso. ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.11737
更新日期:2020-11-30 00:00:00
abstract:INTRODUCTION:The aim of this study was to assess tuberculosis (TB) knowledge, attitudes, and practices in both the general population and risk groups in Thailand. METHODOLOGY:In a cross-sectional survey, a general population (n = 3,074) and family members of a TB patient (n = 559) were randomly selected, using stratif...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6963
更新日期:2016-08-02 00:00:00
abstract:INTRODUCTION:Diarrhea remains one of the main causes of morbidity and mortality in children under five years of age especially in low-income countries. In Peru, epidemiological reports about enteropathogens related to acute diarrhea are scarce in rural areas. The aim of this study was to describe the etiology, epidemio...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.7881
更新日期:2017-12-10 00:00:00
abstract:INTRODUCTION:Human co-infection with Plasmodium falciparum and helminthes is ubiquitous throughout Africa. This study aimed to determine the co-infections of Plasmodium falciparum infection in HIV and intestinal parasitic infections, and their immunological distribution, in Benin City, Nigeria. METHODOLOGY:A total of ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1889
更新日期:2012-05-14 00:00:00