当前位置： SCI文献检索 > PLoS Neglected Tropical Diseases期刊下所有文献 > Easy identification of leishmania species by mass spectrometry.

Easy identification of leishmania species by mass spectrometry.

Abstract：

BACKGROUND:Cutaneous leishmaniasis is caused by several Leishmania species that are associated with variable outcomes before and after therapy. Optimal treatment decision is based on an accurate identification of the infecting species but current methods to type Leishmania isolates are relatively complex and/or slow. Therefore, the initial treatment decision is generally presumptive, the infecting species being suspected on epidemiological and clinical grounds. A simple method to type cultured isolates would facilitate disease management. METHODOLOGY:We analyzed MALDI-TOF spectra of promastigote pellets from 46 strains cultured in monophasic medium, including 20 short-term cultured isolates from French travelers (19 with CL, 1 with VL). As per routine procedure, clinical isolates were analyzed in parallel with Multilocus Sequence Typing (MLST) at the National Reference Center for Leishmania. PRINCIPAL FINDINGS:Automatic dendrogram analysis generated a classification of isolates consistent with reference determination of species based on MLST or hsp70 sequencing. A minute analysis of spectra based on a very simple, database-independent analysis of spectra based on the algorithm showed that the mutually exclusive presence of two pairs of peaks discriminated isolates considered by reference methods to belong either to the Viannia or Leishmania subgenus, and that within each subgenus presence or absence of a few peaks allowed discrimination to species complexes level. CONCLUSIONS/SIGNIFICANCE:Analysis of cultured Leishmania isolates using mass spectrometry allows a rapid and simple classification to the species complex level consistent with reference methods, a potentially useful method to guide treatment decision in patients with cutaneous leishmaniasis.

journal_name

PLoS Negl Trop Dis

journal_title

PLoS neglected tropical diseases

authors

Mouri O,Morizot G,Van der Auwera G,Ravel C,Passet M,Chartrel N,Joly I,Thellier M,Jauréguiberry S,Caumes E,Mazier D,Marinach-Patrice C,Buffet P

doi

10.1371/journal.pntd.0002841

subject

Has Abstract

pub_date

2014-06-05 00:00:00

pages

e2841

issue

6

eissn

1935-2727

issn

1935-2735

pii

PNTD-D-13-01083

journal_volume

8

pub_type

杂志文章

相关文献

PLoS Neglected Tropical Diseases文献大全
  • Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq.

    abstract：BACKGROUND:Filarial nematodes currently infect up to 54 million people worldwide, with millions more at risk for infection, representing the leading cause of disability in the developing world. Brugia malayi is one of the causative agents of lymphatic filariasis and remains the only human filarial parasite that can be ...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0005357

    authors： Grote A,Voronin D,Ding T,Twaddle A,Unnasch TR,Lustigman S,Ghedin E

    更新日期：2017-03-30 00:00:00

  • Neutralization of cholera toxin with nanoparticle decoys for treatment of cholera.

    abstract：:Diarrheal diseases are a major cause of morbidity and mortality worldwide. In many cases, antibiotic therapy is either ineffective or not recommended due to concerns about emergence of resistance. The pathogenesis of several of the most prevalent infections, including cholera and enteroxigenic Escherichia coli, is dom...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0006266

    authors： Das S,Angsantikul P,Le C,Bao D,Miyamoto Y,Gao W,Zhang L,Eckmann L

    更新日期：2018-02-22 00:00:00

  • Interaction of ATP with a small heat shock protein from Mycobacterium leprae: effect on its structure and function.

    abstract：:Adenosine-5'-triphosphate (ATP) is an important phosphate metabolite abundantly found in Mycobacterium leprae bacilli. This pathogen does not derive ATP from its host but has its own mechanism for the generation of ATP. Interestingly, this molecule as well as several antigenic proteins act as bio-markers for the detec...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0003661

    authors： Nandi SK,Chakraborty A,Panda AK,Ray SS,Kar RK,Bhunia A,Biswas A

    更新日期：2015-03-26 00:00:00

  • Using Mobile Health (mHealth) and geospatial mapping technology in a mass campaign for reactive oral cholera vaccination in rural Haiti.

    abstract：BACKGROUND:In mass vaccination campaigns, large volumes of data must be managed efficiently and accurately. In a reactive oral cholera vaccination (OCV) campaign in rural Haiti during an ongoing epidemic, we used a mobile health (mHealth) system to manage data on 50,000 participants in two isolated communities. METHOD...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0003050

    authors： Teng JE,Thomson DR,Lascher JS,Raymond M,Ivers LC

    更新日期：2014-07-31 00:00:00

  • A non mouse-adapted dengue virus strain as a new model of severe dengue infection in AG129 mice.

    abstract：:The spread of dengue (DEN) worldwide combined with an increased severity of the DEN-associated clinical outcomes have made this mosquito-borne virus of great global public health importance. Progress in understanding DEN pathogenesis and in developing effective treatments has been hampered by the lack of a suitable sm...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0000672

    authors： Tan GK,Ng JK,Trasti SL,Schul W,Yip G,Alonso S

    更新日期：2010-04-27 00:00:00

  • Socioeconomic risk markers of leprosy in high-burden countries: A systematic review and meta-analysis.

    abstract：:Over 200,000 new cases of leprosy are detected each year, of which approximately 7% are associated with grade-2 disabilities (G2Ds). For achieving leprosy elimination, one of the main challenges will be targeting higher risk groups within endemic communities. Nevertheless, the socioeconomic risk markers of leprosy rem...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章,meta分析,评审

    doi：10.1371/journal.pntd.0006622

    authors： Pescarini JM,Strina A,Nery JS,Skalinski LM,Andrade KVF,Penna MLF,Brickley EB,Rodrigues LC,Barreto ML,Penna GO

    更新日期：2018-07-09 00:00:00

  • Detection of Bartonella spp. in fleas by MALDI-TOF MS.

    abstract：BACKGROUND:Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has recently emerged in the field of entomology as a promising method for the identification of arthropods and the detection of associated pathogens. METHODOLOGY/PRINCIPAL FINDINGS:An experimental model of Ctenocepha...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0006189

    authors： El Hamzaoui B,Laroche M,Almeras L,Bérenger JM,Raoult D,Parola P

    更新日期：2018-02-16 00:00:00

  • Correction: Prevalence of Lymphatic Filariasis and Treatment Effectiveness of Albendazole/ Ivermectin in Individuals with HIV Co-infection in Southwest-Tanzania.

    abstract：:[This corrects the article DOI: 10.1371/journal.pntd.0004618.]. ...

    journal_title：PLoS neglected tropical diseases

    pub_type： 已发布勘误

    doi：10.1371/journal.pntd.0004967

    authors： Kroidl I,Saathoff E,Maganga L,Clowes P,Maboko L,Hoerauf A,Makunde WH,Haule A,Mviombo P,Pitter B,Mgeni N,Mabuye J,Kowuor D,Mwingira U,Malecela MN,Löscher T,Hoelscher M

    更新日期：2016-08-26 00:00:00

  • Broad diversity of Mycobacterium tuberculosis complex strains isolated from humans and cattle in Northern Algeria suggests a zoonotic transmission cycle.

    abstract：:Mycobacterium tuberculosis complex (MTBC) comprises closely related species responsible for human and animal tuberculosis (TB). Efficient species determination is useful for epidemiological purposes, especially for the elucidation of the zoonotic contribution. In Algeria, data on MTBC genotypes are largely unknown. In...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0008894

    authors： Damene H,Tahir D,Diels M,Berber A,Sahraoui N,Rigouts L

    更新日期：2020-11-30 00:00:00

  • Latent-class methods to evaluate diagnostics tests for Echinococcus infections in dogs.

    abstract：BACKGROUND:The diagnosis of canine echinococcosis can be a challenge in surveillance studies because there is no perfect gold standard that can be used routinely. However, unknown test specificities and sensitivities can be overcome using latent-class analysis with appropriate data. METHODOLOGY:We utilised a set of fa...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0002068

    authors： Hartnack S,Budke CM,Craig PS,Jiamin Q,Boufana B,Campos-Ponce M,Torgerson PR

    更新日期：2013-01-01 00:00:00

  • Trypanosoma cruzi CYP51 inhibitor derived from a Mycobacterium tuberculosis screen hit.

    abstract：BACKGROUND:The two front-line drugs for chronic Trypanosoma cruzi infections are limited by adverse side-effects and declining efficacy. One potential new target for Chagas' disease chemotherapy is sterol 14alpha-demethylase (CYP51), a cytochrome P450 enzyme involved in biosynthesis of membrane sterols. METHODOLOGY/PR...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0000372

    authors： Chen CK,Doyle PS,Yermalitskaya LV,Mackey ZB,Ang KK,McKerrow JH,Podust LM

    更新日期：2009-01-01 00:00:00

  • Cell-mediated and serology-based tests for Mycobacterium ulcerans disease: A systematic review and meta-analysis.

    abstract：:Buruli ulcer (BU) is a subcutaneous necrotic infection of the skin caused by Mycobacterium ulcerans. It is the third most common human mycobacterial disease after tuberculosis (TB) and leprosy. The available methods for detection of the bacilli in lesions are microscopic detection, isolation and cultivation of the bac...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章,meta分析

    doi：10.1371/journal.pntd.0008172

    authors： Avumegah MS,Waidyatillake NT,Michalski WP,O'Brien DP,Nelson TM,Athan E

    更新日期：2020-04-06 00:00:00

  • Activation of indoleamine 2,3-dioxygenase in patients with scrub typhus and its role in growth restriction of Orientia tsutsugamushi.

    abstract：BACKGROUND:Our earlier genome-wide expression study revealed up-regulation of a tryptophan-catabolizing enzyme, indoleamine 2,3-dioxygenase (IDO1), in patients with scrub typhus. This gene has been previously reported to have anti-microbial activity in a variety of infectious diseases; therefore, we aimed to prove whet...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0001731

    authors： Prachason T,Konhan K,Pongnarin P,Chatsiricharoenkul S,Suputtamongkol Y,Limwongse C

    更新日期：2012-01-01 00:00:00

  • Therapeutic control of leishmaniasis by inhibitors of the mammalian target of rapamycin.

    abstract：:Leishmaniasis is a serious global health problem affecting many people worldwide. While patients with leishmaniasis can be treated with several agents, drug toxicicty and the emergence of resistant strains render available treatments ineffective in the long run. Inhibitors of the mammalian target of rapamycin (mTOR) h...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0006701

    authors： Khadir F,Shaler CR,Oryan A,Rudak PT,Mazzuca DM,Taheri T,Dikeakos JD,Haeryfar SMM,Rafati S

    更新日期：2018-08-22 00:00:00

  • Clinical and molecular epidemiology of Crimean-Congo hemorrhagic fever in Oman.

    abstract：BACKGROUND:Crimean-Congo hemorrhagic fever (CCHF) is a serious disease with a high fatality rate reported in many countries. The first case of CCHF in Oman was detected in 1995 and serosurveys have suggested widespread infection of humans and livestock throughout the country. METHODOLOGY:Cases of CCHF reported to the ...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0007100

    authors： Al-Abri SS,Hewson R,Al-Kindi H,Al-Abaidani I,Al-Jardani A,Al-Maani A,Almahrouqi S,Atkinson B,Al-Wahaibi A,Al-Rawahi B,Bawikar S,Beeching NJ

    更新日期：2019-04-25 00:00:00

  • Characterization of schistosome tegumental alkaline phosphatase (SmAP).

    abstract：:Schistosomes are parasitic platyhelminths that currently infect over 200 million people globally. The parasites can live for years in a putatively hostile environment - the blood of vertebrates. We have hypothesized that the unusual schistosome tegument (outer-covering) plays a role in protecting parasites in the bloo...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0001011

    authors： Bhardwaj R,Skelly PJ

    更新日期：2011-04-05 00:00:00

  • A randomized trial of AmBisome monotherapy and AmBisome and miltefosine combination to treat visceral leishmaniasis in HIV co-infected patients in Ethiopia.

    abstract：BACKGROUND:Visceral leishmaniasis (VL) in human immunodeficiency virus (HIV) co-infected patients requires special case management. AmBisome monotherapy at 40 mg/kg is recommended by the World Health Organization. The objective of the study was to assess if a combination of a lower dose of AmBisome with miltefosine wou...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章,随机对照试验

    doi：10.1371/journal.pntd.0006988

    authors： Diro E,Blesson S,Edwards T,Ritmeijer K,Fikre H,Admassu H,Kibret A,Ellis SJ,Bardonneau C,Zijlstra EE,Soipei P,Mutinda B,Omollo R,Kimutai R,Omwalo G,Wasunna M,Tadesse F,Alves F,Strub-Wourgaft N,Hailu A,Alexander N,

    更新日期：2019-01-17 00:00:00

  • Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru.

    abstract：BACKGROUND:Transmission of dengue virus (DENV) from humans to mosquitoes represents a critical component of dengue epidemiology. Examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of DENV by mosquitoes from humans. In this study, we assessed arti...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0007116

    authors： Long KC,Sulca J,Bazan I,Astete H,Jaba HL,Siles C,Kocher C,Vilcarromero S,Schwarz J,Escobedo-Vargas KS,Castro-Llanos F,Angulo L,Flores G,Ramal-Asayag C,Halsey ES,Hontz RD,Paz-Soldan VA,Scott TW,Lambrechts L,Morrison

    更新日期：2019-02-12 00:00:00

  • Antibodies against immature virions are not a discriminating factor for dengue disease severity.

    abstract：:Humoral immunity plays an important role in controlling dengue virus (DENV) infection. Antibodies (Abs) developed during primary infection protect against subsequent infection with the same dengue serotype, but can enhance disease following secondary infection with a heterologous serotype. A DENV virion has two surfac...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0003564

    authors： Rodenhuis-Zybert IA,da Silva Voorham JM,Torres S,van de Pol D,Smit JM

    更新日期：2015-03-11 00:00:00

  • Assay strategies for the discovery and validation of therapeutics targeting Brugia pahangi Hsp90.

    abstract：:The chemotherapy of lymphatic filariasis relies upon drugs such as diethylcarbamazine and ivermectin that largely target the microfilarial stages of the parasite, necessitating continued treatment over the long reproductive life span of the adult worm. The identification of compounds that target adult worms has been a...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0000714

    authors： Taldone T,Gillan V,Sun W,Rodina A,Patel P,Maitland K,O'Neill K,Chiosis G,Devaney E

    更新日期：2010-06-15 00:00:00

  • Stress susceptibility in Trypanosoma brucei lacking the RNA-binding protein ZC3H30.

    abstract：:Trypanosomes rely on post-transcriptional mechanisms and mRNA-binding proteins for control of gene expression. Trypanosoma brucei ZC3H30 is an mRNA-binding protein that is expressed in both the bloodstream form (which grows in mammals) and the procyclic form (which grows in the tsetse fly midgut). Attachment of ZC3H30...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0006835

    authors： Chakraborty C,Clayton C

    更新日期：2018-10-01 00:00:00

  • Common coinfections of Giardia intestinalis and Helicobacter pylori in non-symptomatic Ugandan children.

    abstract：BACKGROUND:The protozoan parasite Giardia intestinalis and the pathogenic bacterium Helicobacter pylori are well known for their high prevalences in human hosts worldwide. The prevalence of both organisms is known to peak in densely populated, low resource settings and children are infected early in life. Different Gia...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0001780

    authors： Ankarklev J,Hestvik E,Lebbad M,Lindh J,Kaddu-Mulindwa DH,Andersson JO,Tylleskär T,Tumwine JK,Svärd SG

    更新日期：2012-01-01 00:00:00

  • A systematic review of the epidemiology of echinococcosis in domestic and wild animals.

    abstract：BACKGROUND:Human echinococcosis is a neglected zoonosis caused by parasites of the genus Echinococcus. The most frequent clinical forms of echinococcosis, cystic echinococcosis (CE) and alveolar echinococcosis (AE), are responsible for a substantial health and economic burden, particularly to low-income societies. Quan...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章,评审

    doi：10.1371/journal.pntd.0002249

    authors： Otero-Abad B,Torgerson PR

    更新日期：2013-06-06 00:00:00

  • Biodegradable nanocarriers coated with polymyxin B: Evaluation of leishmanicidal and antibacterial potential.

    abstract：:Most treatments of leishmaniasis require hospitalization and present side effects or parasite resistance; innovations in drug formulation/reposition can overcome these barriers and must be pursued to increase therapeutic alternatives. Therefore, we tested polymyxin B (polB) potential to kill Leishmania amazonensis, ad...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0007388

    authors： Souza Ribeiro Costa J,Medeiros M,Yamashiro-Kanashiro EH,Rocha MC,Cotrim PC,Stephano MA,Lancellotti M,Tavares GD,Oliveira-Nascimento L

    更新日期：2019-05-01 00:00:00

  • Congenital toxoplasmosis in Austria: Prenatal screening for prevention is cost-saving.

    abstract：BACKGROUND:Primary infection of Toxoplasma gondii during pregnancy can be transmitted to the unborn child and may have serious consequences, including retinochoroiditis, hydrocephaly, cerebral calcifications, encephalitis, splenomegaly, hearing loss, blindness, and death. Austria, a country with moderate seroprevalence...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0005648

    authors： Prusa AR,Kasper DC,Sawers L,Walter E,Hayde M,Stillwaggon E

    更新日期：2017-07-10 00:00:00

  • Epidemiological characterization of incident cases of Rickettsia infection in rural areas of Urabá region, Colombia.

    abstract：INTRODUCTION:Most of the studies related to rickettsial infection in Colombia are cross-sectional because of the challenge in conducting prospective studies on infectious disease that may have a difficult diagnosis. Although cross-sectional studies are essential to detect people exposed to rickettsiae, they are not sui...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0006911

    authors： Quintero Vélez JC,Aguirre-Acevedo DC,Rodas JD,Arboleda M,Troyo A,Vega Aguilar F,Osorio Quintero L,Rojas Arbeláez C

    更新日期：2018-10-31 00:00:00

  • Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini.

    abstract：BACKGROUND:The liver fluke Opisthorchis viverrini is classified as a class I carcinogen due to the association between cholangiocarcinoma and chronic O. viverrini infection. During its feeding activity within the bile duct, the parasite secretes several cathepsin F cysteine proteases that may induce or contribute to th...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0000398

    authors： Pinlaor P,Kaewpitoon N,Laha T,Sripa B,Kaewkes S,Morales ME,Mann VH,Parriott SK,Suttiprapa S,Robinson MW,To J,Dalton JP,Loukas A,Brindley PJ

    更新日期：2009-01-01 00:00:00

  • Anopheles gambiae antiviral immune response to systemic O'nyong-nyong infection.

    abstract：BACKGROUND:Mosquito-borne viral diseases cause significant burden in much of the developing world. Although host-virus interactions have been studied extensively in the vertebrate host, little is known about mosquito responses to viral infection. In contrast to mosquitoes of the Aedes and Culex genera, Anopheles gambia...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0001565

    authors： Waldock J,Olson KE,Christophides GK

    更新日期：2012-01-01 00:00:00

  • Access to Chagas disease treatment in the United States after the regulatory approval of benznidazole.

    abstract：:Approximately 300,000 persons in the United States (US) are infected with Trypanosoma cruzi, the protozoan that causes Chagas disease, but less than 1% are estimated to have received antiparasitic treatment. Benznidazole was approved by the US Food and Drug Administration (FDA) for treatment of T. cruzi infection in 2...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0008398

    authors： Yoshioka K,Manne-Goehler J,Maguire JH,Reich MR

    更新日期：2020-06-22 00:00:00

  • An mRNA Vaccine Encoding Rabies Virus Glycoprotein Induces Protection against Lethal Infection in Mice and Correlates of Protection in Adult and Newborn Pigs.

    abstract：:Rabies is a zoonotic infectious disease of the central nervous system (CNS). In unvaccinated or untreated subjects, rabies virus infection causes severe neurological symptoms and is invariably fatal. Despite the long-standing existence of effective vaccines, vaccine availability remains insufficient, with high numbers...

    journal_title：PLoS neglected tropical diseases

    pub_type： 杂志文章

    doi：10.1371/journal.pntd.0004746

    authors： Schnee M,Vogel AB,Voss D,Petsch B,Baumhof P,Kramps T,Stitz L

    更新日期：2016-06-23 00:00:00