Feasibility of feeding Aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in Iquitos, Peru.


BACKGROUND:Transmission of dengue virus (DENV) from humans to mosquitoes represents a critical component of dengue epidemiology. Examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of DENV by mosquitoes from humans. In this study, we assessed artificial and natural blood feeding methods based on rates of DENV infection and dissemination within mosquitoes for use in a field-based epidemiological cohort study in Iquitos, Peru. METHODOLOGY/PRINCIPAL FINDINGS:Our study was implemented, stepwise, between 2011 and 2015. Participants who were 5 years and older with 5 or fewer days of fever were enrolled from ongoing clinic- and neighborhood-based studies on dengue in Iquitos. Wild type, laboratory-reared Aedes aegypti were fed directly on febrile individuals or on blood collected from participants that was either untreated or treated with EDTA. Mosquitoes were tested after approximately 14 days of extrinsic incubation for DENV infection and dissemination. A total of 58 participants, with viremias ranging from 1.3 × 10(2) to 2.9 × 10(6) focus-forming units per mL of serum, participated in one or more feeding methods. DENV infection and dissemination rates were not significantly different following direct and indirect-EDTA feeding; however, they were significantly lower for mosquitoes that fed indirectly on blood with no additive. Relative to direct feeding, infection rates showed greater variation following indirect-EDTA than indirect-no additive feeding. Dissemination rates were similar across all feeding methods. No differences were detected in DENV infection or dissemination rates in mosquitoes fed directly on participants with different dengue illness severity. CONCLUSIONS/SIGNIFICANCE:Our study demonstrates the feasibility of using direct and indirect feeding methods for field-based studies on vector competence. Direct mosquito feeding is preferable in terms of logistical ease, biosecurity, and reliability.


PLoS Negl Trop Dis


Long KC,Sulca J,Bazan I,Astete H,Jaba HL,Siles C,Kocher C,Vilcarromero S,Schwarz J,Escobedo-Vargas KS,Castro-Llanos F,Angulo L,Flores G,Ramal-Asayag C,Halsey ES,Hontz RD,Paz-Soldan VA,Scott TW,Lambrechts L,Morrison




Has Abstract


2019-02-12 00:00:00














  • Optimization of a Membrane Feeding Assay for Plasmodium vivax Infection in Anopheles albimanus.

    abstract:INTRODUCTION:Individuals exposed to malaria infections for a long time develop immune responses capable of blocking Plasmodium transmission to mosquito vectors, potentially limiting parasite spreading in nature. Development of a malaria TB vaccine requires a better understanding of the mechanisms and main effectors res...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Vallejo AF,Rubiano K,Amado A,Krystosik AR,Herrera S,Arévalo-Herrera M

    更新日期:2016-06-29 00:00:00

  • Detection of clinical and neurological signs in apparently asymptomatic HTLV-1 infected carriers: Association with high proviral load.

    abstract::Several studies suggest that HTLV-1 infection may be associated with a wider spectrum of neurologic manifestations that do not meet diagnostic criteria for HAM/TSP. These conditions may later progress to HAM/TSP or constitute an intermediate clinical form, between asymptomatic HTLV-1 carriers and those with full myelo...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Haziot ME,Gascon MR,Assone T,Fonseca LAM,Luiz ODC,Smid J,Paiva AM,Marcusso RMDN,de Oliveira ACP,Casseb J

    更新日期:2019-05-01 00:00:00

  • Prevalence of chronic chikungunya and associated risks factors in the French West Indies (La Martinique): A prospective cohort study.

    abstract:BACKGROUND:The chikungunya virus (CHIKV) is a re-emerging alphavirus that can cause chronic and potentially incapacitating rheumatic musculoskeletal disorders known as chronic chikungunya arthritis (CCA). We conducted a prospective cohort study of CHIKV-infected subjects during the 2013 chikungunya outbreak in Martiniq...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Bertolotti A,Thioune M,Abel S,Belrose G,Calmont I,Césaire R,Cervantes M,Fagour L,Javelle É,Lebris C,Najioullah F,Pierre-François S,Rozé B,Vigan M,Laouénan C,Cabié A,Chronic Chikungunya working group of University Medical

    更新日期:2020-03-12 00:00:00

  • Community knowledge, attitude, and perceived stigma of leprosy amongst community members living in Dhanusha and Parsa districts of Southern Central Nepal.

    abstract:BACKGROUND:Though Nepal declared leprosy elimination in 2010, its burden is constantly rising in Terai communities for the past 2 years with 3000 new leprosy cases being diagnosed annually. Community's perception is important for prevention and control of leprosy and enhancing quality of life of leprosy patients. Poor ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Singh R,Singh B,Mahato S

    更新日期:2019-01-11 00:00:00

  • Early Indicators of Fatal Leptospirosis during the 2010 Epidemic in Puerto Rico.

    abstract:BACKGROUND:Leptospirosis is a potentially fatal bacterial zoonosis that is endemic throughout the tropics and may be misdiagnosed as dengue. Delayed hospital admission of leptospirosis patients is associated with increased mortality. METHODOLOGY/PRINCIPAL FINDINGS:During a concurrent dengue/leptospirosis epidemic in P...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Sharp TM,Rivera García B,Pérez-Padilla J,Galloway RL,Guerra M,Ryff KR,Haberling D,Ramakrishnan S,Shadomy S,Blau D,Tomashek KM,Bower WA

    更新日期:2016-02-25 00:00:00

  • Correction: Schistosoma species detection by environmental DNA assays in African freshwaters.

    abstract::[This corrects the article DOI: 10.1371/journal.pntd.0008129.]. ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 已发布勘误


    authors: Alzaylaee H,Collins RA,Rinaldi G,Shechonge A,Ngatunga B,Morgan ER,Genner MJ

    更新日期:2020-09-01 00:00:00

  • Activation of indoleamine 2,3-dioxygenase in patients with scrub typhus and its role in growth restriction of Orientia tsutsugamushi.

    abstract:BACKGROUND:Our earlier genome-wide expression study revealed up-regulation of a tryptophan-catabolizing enzyme, indoleamine 2,3-dioxygenase (IDO1), in patients with scrub typhus. This gene has been previously reported to have anti-microbial activity in a variety of infectious diseases; therefore, we aimed to prove whet...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Prachason T,Konhan K,Pongnarin P,Chatsiricharoenkul S,Suputtamongkol Y,Limwongse C

    更新日期:2012-01-01 00:00:00

  • An investig-ation into the epidemiology of chikungunya virus across neglected regions of Indonesia.

    abstract:BACKGROUND:Chikungunya virus (CHIKV) is an important emerging and re-emerging public health problem worldwide. In Indonesia, where the virus is endemic, epidemiological information from outside of the main islands of Java and Bali is limited. METHODOLOGY/PRINCIPAL FINDINGS:Four hundred and seventy nine acutely febrile...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Stubbs SCB,Johar E,Yudhaputri FA,Yohan B,Santoso MS,Hayati RF,Denis D,Blacklaws BA,Powers AM,Sasmono RT,Myint KSA,Frost SDW

    更新日期:2020-12-21 00:00:00

  • Effects of 'The Vicious Worm' educational tool on Taenia solium knowledge retention in Zambian primary school students after one year.

    abstract:BACKGROUND:Taenia solium is a neglected zoonotic parasite endemic throughout many low-income countries worldwide, including Zambia, where it causes human and pig diseases with high health and socioeconomic burdens. Lack of knowledge is a recognized risk factor, and consequently targeted health educational programs can ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Hobbs EC,Mwape KE,Devleesschauwer B,Van Damme I,Krit M,Berkvens D,Zulu G,Mambwe M,Chembensofu M,Trevisan C,Baauw J,Phiri IK,Speybroeck N,Ketzis J,Dorny P,Willingham AL,Gabriël S

    更新日期:2019-05-20 00:00:00

  • Hydrological Regime and Water Shortage as Drivers of the Seasonal Incidence of Diarrheal Diseases in a Tropical Montane Environment.

    abstract:BACKGROUND:The global burden of diarrhea is a leading cause of morbidity and mortality worldwide. In montane areas of South-East Asia such as northern Laos, recent changes in land use have induced increased runoff, soil erosion and in-stream suspended sediment loads, and potential pathogen dissemination. To our knowled...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Boithias L,Choisy M,Souliyaseng N,Jourdren M,Quet F,Buisson Y,Thammahacksa C,Silvera N,Latsachack K,Sengtaheuanghoung O,Pierret A,Rochelle-Newall E,Becerra S,Ribolzi O

    更新日期:2016-12-09 00:00:00

  • Mixed Th1 and Th2 Mycobacterium tuberculosis-specific CD4 T cell responses in patients with active pulmonary tuberculosis from Tanzania.

    abstract::Mycobacterium tuberculosis (Mtb) and helminth infections elicit antagonistic immune effector functions and are co-endemic in several regions of the world. We therefore hypothesized that helminth infection may influence Mtb-specific T-cell immune responses. We evaluated the cytokine profile of Mtb-specific T cells in 7...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Amelio P,Portevin D,Reither K,Mhimbira F,Mpina M,Tumbo A,Nickel B,Marti H,Knopp S,Ding S,Penn-Nicholson A,Darboe F,Ohmiti K,Scriba TJ,Pantaleo G,Daubenberger C,Perreau M

    更新日期:2017-07-31 00:00:00

  • Assessment of control strategies against Clonorchis sinensis infection based on a multi-group dynamic transmission model.

    abstract::Clonorchiasis is one of the most important food-borne trematodiases affecting millions of people. Strategies were recommended by different organizations and control programmes were implemented but mostly in short-time periods. It's important to assess the long-term benefits and sustainability of possible control strat...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Huang XH,Qian MB,Zhu GH,Fang YY,Hao YT,Lai YS

    更新日期:2020-03-27 00:00:00

  • Human african trypanosomiasis diagnosis in first-line health services of endemic countries, a systematic review.

    abstract::While the incidence of Human African Trypanosomiasis (HAT) is decreasing, the control approach is shifting from active population screening by mobile teams to passive case detection in primary care centers. We conducted a systematic review of the literature between 1970 and 2011 to assess which diagnostic tools are mo...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章,评审


    authors: Mitashi P,Hasker E,Lejon V,Kande V,Muyembe JJ,Lutumba P,Boelaert M

    更新日期:2012-01-01 00:00:00

  • Neotropical bats that co-habit with humans function as dead-end hosts for dengue virus.

    abstract::Several studies have shown Dengue Virus (DENV) nucleic acids and/or antibodies present in Neotropical wildlife including bats, suggesting that some bat species may be susceptible to DENV infection. Here we aim to elucidate the role of house-roosting bats in the DENV transmission cycle. Bats were sampled in households ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Vicente-Santos A,Moreira-Soto A,Soto-Garita C,Chaverri LG,Chaves A,Drexler JF,Morales JA,Alfaro-Alarcón A,Rodríguez-Herrera B,Corrales-Aguilar E

    更新日期:2017-05-18 00:00:00

  • Impact of a Reference Center on Leprosy Control under a Decentralized Public Health Care Policy in Brazil.

    abstract:OBJECTIVE:We evaluated the profile of patients referred to the Fiocruz Outpatient Clinic, a reference center for the diagnosis and treatment of leprosy in Rio de Janeiro, RJ, and analyzed the origins and outcomes of these referrals. METHODS:This is an observational retrospective study based on information collected fr...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Barbieri RR,Sales AM,Hacker MA,Nery JA,Duppre NC,Machado AM,Moraes MO,Sarno EN

    更新日期:2016-10-12 00:00:00

  • Chaetomium atrobrunneum causing human eumycetoma: The first report.

    abstract::In this communication, a case of black grain eumycetoma produced by the fungus C. atrobrunneum is reported. The patient was initially misdiagnosed with M. mycetomatis eumycetoma based on the grains' morphological and cytological features. However, further aerobic culture of the black grains generated a melanised fungu...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Mhmoud NA,Santona A,Fiamma M,Siddig EE,Deligios M,Bakhiet SM,Rubino S,Fahal AH

    更新日期:2019-05-30 00:00:00

  • Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively.

    abstract:BACKGROUND:Treponema pallidum subsp. endemicum (TEN) is the causative agent of endemic syphilis (bejel). An unusual human TEN 11q/j isolate was obtained from a syphilis-like primary genital lesion from a patient that returned to France from Pakistan. METHODOLOGY/PRINCIPAL FINDINGS:The TEN 11q/j isolate was characteriz...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Mikalová L,Strouhal M,Oppelt J,Grange PA,Janier M,Benhaddou N,Dupin N,Šmajs D

    更新日期:2017-03-06 00:00:00

  • Trypanosoma cruzi I and IV stocks from Brazilian Amazon are divergent in terms of biological and medical properties in mice.

    abstract:BACKGROUND:In the Brazilian Amazon, clinical and epidemiological frameworks of Chagas disease are very dissimilar in relation to the endemic classical areas of transmission, possibly due to genetic and biological characteristics of the circulating Trypanosoma cruzi stocks. Twenty six T. cruzi stocks from Western Amazon...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Monteiro WM,Margioto Teston AP,Gruendling AP,dos Reis D,Gomes ML,de Araújo SM,Bahia MT,Magalhães LK,de Oliveira Guerra JA,Silveira H,Toledo MJ,Vale Barbosa Md

    更新日期:2013-01-01 00:00:00

  • Modeling to predict cases of hantavirus pulmonary syndrome in Chile.

    abstract:BACKGROUND:Hantavirus pulmonary syndrome (HPS) is a life threatening disease transmitted by the rodent Oligoryzomys longicaudatus in Chile. Hantavirus outbreaks are typically small and geographically confined. Several studies have estimated risk based on spatial and temporal distribution of cases in relation to climate...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Nsoesie EO,Mekaru SR,Ramakrishnan N,Marathe MV,Brownstein JS

    更新日期:2014-04-24 00:00:00

  • IL-33/ST2 correlates with severity of haemorrhagic fever with renal syndrome and regulates the inflammatory response in Hantaan virus-infected endothelial cells.

    abstract:BACKGROUND:Hantaan virus (HTNV) causes a severe lethal haemorrhagic fever with renal syndrome (HFRS) in humans. Despite a limited understanding of the pathogenesis of HFRS, the importance of the abundant production of pro-inflammatory cytokines has been widely recognized. Interleukin 33 (IL-33) has been demonstrated to...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Zhang Y,Zhang C,Zhuang R,Ma Y,Zhang Y,Yi J,Yang A,Jin B

    更新日期:2015-02-06 00:00:00

  • Projecting the long-term impact of school- or community-based mass-treatment interventions for control of Schistosoma infection.

    abstract:BACKGROUND:Schistosomiasis remains a significant health burden in many areas of the world. Morbidity control, focused on limiting infection intensity through periodic delivery of anti-schistosomal medicines, is the thrust of current World Health Organization guidelines (2006) for reduction of Schistosoma-related diseas...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Wang X,Gurarie D,Mungai PL,Muchiri EM,Kitron U,King CH

    更新日期:2012-01-01 00:00:00

  • Isothermal microcalorimetry, a new tool to monitor drug action against Trypanosoma brucei and Plasmodium falciparum.

    abstract::Isothermal microcalorimetry is an established tool to measure heat flow of physical, chemical or biological processes. The metabolism of viable cells produces heat, and if sufficient cells are present, their heat production can be assessed by this method. In this study, we investigated the heat flow of two medically i...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Wenzler T,Steinhuber A,Wittlin S,Scheurer C,Brun R,Trampuz A

    更新日期:2012-01-01 00:00:00

  • Modeling Powassan virus infection in Peromyscus leucopus, a natural host.

    abstract::The tick-borne flavivirus, Powassan virus (POWV) causes life-threatening encephalitis in humans in North America and Europe. POWV is transmitted by ixodid tick vectors that feed on small to medium-sized mammals, such as Peromyscus leucopus mice, which may serve as either reservoir, bridge or amplification hosts. Intra...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Mlera L,Meade-White K,Saturday G,Scott D,Bloom ME

    更新日期:2017-01-31 00:00:00

  • Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos.

    abstract::Mycobacterium leprae (M. leprae) is a human pathogen and the causative agent for leprosy, a chronic disease characterized by lesions of the skin and peripheral nerve damage. Zoonotic transmission of M. leprae to humans by nine-banded armadillos (Dasypus novemcinctus) has been shown to occur in the southern United Stat...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: da Silva MB,Portela JM,Li W,Jackson M,Gonzalez-Juarrero M,Hidalgo AS,Belisle JT,Bouth RC,Gobbo AR,Barreto JG,Minervino AHH,Cole ST,Avanzi C,Busso P,Frade MAC,Geluk A,Salgado CG,Spencer JS

    更新日期:2018-06-28 00:00:00

  • A voluntary use of insecticide treated nets can stop the vector transmission of Chagas disease.

    abstract::One of the stated goals of the London Declaration on Neglected Tropical Diseases is the interruption of domiciliary transmissions of Chagas disease in the region of the Americas. We used a game-theoretic approach to assess the voluntary use of insecticide treated nets (ITNs) in the prevention of the spread of infectio...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Han CY,Issa H,Rychtář J,Taylor D,Umana N

    更新日期:2020-11-03 00:00:00

  • Epidemiologic Correlates of Mortality among Symptomatic Visceral Leishmaniasis Cases: Findings from Situation Assessment in High Endemic Foci in India.

    abstract:BACKGROUND:Visceral leishmaniasis (VL) is highly prevalent in the Indian state of Bihar and, without proper diagnosis and treatment, is associated with high fatality. However, lack of efficient reporting mechanism had been an impediment in estimating the burden of mortality and its antecedents among symptomatic VL case...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Das A,Karthick M,Dwivedi S,Banerjee I,Mahapatra T,Srikantiah S,Chaudhuri I

    更新日期:2016-11-21 00:00:00

  • Zika virus infection in the Veterans Health Administration (VHA), 2015-2016.

    abstract:BACKGROUND:Zika virus (ZIKV) is an important flavivirus infection. Although ZIKV infection is rarely fatal, risk for severe disease in adults is not well described. Our objective was to describe the spectrum of illness in U.S. Veterans with ZIKV infection. METHODOLOGY:Case series study including patients with laborato...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Schirmer PL,Wendelboe A,Lucero-Obusan CA,Ryono RA,Winters MA,Oda G,Martinez M,Saavedra S,Holodniy M

    更新日期:2018-05-24 00:00:00

  • Misconceptions and paradoxes in soil-transmitted helminthiases control as a public health problem.

    abstract::Soil-transmitted helminthiases (STHs) constitute a public health problem that requires immediate action to resolve the morbidity of those harboring the parasites in their guts, to prevent infection in all those at risk, and to interrupt the vicious circle of poverty and disease in the affected communities, structural ...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Krolewiecki AJ

    更新日期:2018-09-13 00:00:00

  • Landscape changes influence the occurrence of the melioidosis bacterium Burkholderia pseudomallei in soil in northern Australia.

    abstract:BACKGROUND:The soil-dwelling saprophyte bacterium Burkholderia pseudomallei is the cause of melioidosis, a severe disease of humans and animals in southeast Asia and northern Australia. Despite the detection of B. pseudomallei in various soil and water samples from endemic areas, the environmental habitat of B. pseudom...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Kaestli M,Mayo M,Harrington G,Ward L,Watt F,Hill JV,Cheng AC,Currie BJ

    更新日期:2009-01-01 00:00:00

  • Targeting trachoma control through risk mapping: the example of Southern Sudan.

    abstract:BACKGROUND:Trachoma is a major cause of blindness in Southern Sudan. Its distribution has only been partially established and many communities in need of intervention have therefore not been identified or targeted. The present study aimed to develop a tool to improve targeting of survey and control activities. METHODS...

    journal_title:PLoS neglected tropical diseases

    pub_type: 杂志文章


    authors: Clements AC,Kur LW,Gatpan G,Ngondi JM,Emerson PM,Lado M,Sabasio A,Kolaczinski JH

    更新日期:2010-08-17 00:00:00