Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing.

Abstract:

INTRODUCTION:We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009.  METHODOLOGY:This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing. RESULTS:From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation  using pyrosequencing. CONCLUSIONS:The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs.

journal_name

J Infect Dev Ctries

authors

Chantratita W,Sukasem C,Sirinavin S,Sankuntaw N,Srichantaratsamee C,Pasomsub E,Malathum K

doi

10.3855/jidc.1197

subject

Has Abstract

pub_date

2011-05-28 00:00:00

pages

348-52

issue

5

eissn

2036-6590

issn

1972-2680

journal_volume

5

pub_type

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