Abstract:
BACKGROUND:Streptococcus pneumoniae is a major human pathogen. The emergence of penicillin resistant strains since the 1970s has been life threatening and the evolution of the bacteria have enabled itself to develop resistance to many other antibiotics such as the macrolides and the fluoroquinolones. This study aims to characterize S. pneumoniae isolates for the presence of penicillin and macrolide resistance genes. METHODOLOGY:One hundred and twenty clinical isolates of S. pneumoniae were obtained from patients of University Malaya Medical Centre (UMMC). The strains were screened using a multiplex real-time PCR method for the presence of alterations in the genes encoding the penicillin binding proteins: pbp2b, macrolide resistance determinant ermB and the pneumolysin gene, ply. Dual-labelled Taqman probes were used in the real-time detection method comprising three different genes labeled with individual fluorophores at different wavelengths. One hundred and twenty isolates from bacterial cultures and isolates directly from blood cultures samples were analyzed using this assay. RESULTS:A multiplex PCR comprising the antibiotic resistance genes, ermB and and pneumolysin gene (ply), a S. pneumoniae species specific gene, was developed to characterize strains of S. pneumoniae. Out of the 120 pneumococcal isolates, 58 strains were categorized as Penicillin Sensitive Streptococcus pneumoniae (PSSP), 36 as Penicillin Intermediate Streptococcus pneumoniae (PISP) and 26 as Penicillin Resistant Streptococcus pneumoniae (PRSP). All the 58 PSSP strains harboured the pbp2b gene while the 36 PISP and 26 PRSP strains did not harbour this gene, thus suggesting reduced susceptibility to penicillin. Resistance to erythromycin was observed in 47 of the pneumococcal strains while 15 and 58 were intermediate and sensitive to this drug respectively. Susceptibility testing to other beta-lactams (CTX and CRO) also showed reduced susceptibility among the strains within the PISP and PRSP groups but most PSSP strains were sensitive to other antibiotics. CONCLUSION:The characterization of pneumococcal isolates for penicillin and erythromycin resistance genes could be useful to predict the susceptibility of these isolates to other antibiotics, especially beta-lactams drugs. We have developed an assay with a shorter turnaround time to determine the species and resistance profile of Streptococcus pneumoniae with respect to penicillin and macrolides using the Real Time PCR format with fluorescent labeled Taqman probes, hence facilitating earlier and more definitive antimicrobial therapy which may lead to better patient management.
journal_name
J Infect Dev Ctriesjournal_title
Journal of infection in developing countriesauthors
Kumari N,Navaratnam P,Sekaran SDdoi
10.3855/jidc.262subject
Has Abstractpub_date
2008-06-01 00:00:00pages
193-9issue
3eissn
2036-6590issn
1972-2680journal_volume
2pub_type
杂志文章abstract:INTRODUCTION:Nosocomial pneumonia remains an important cause of mortality and morbidity worldwide. Surveillance programs play an important role in the identification of common etiologic agents and local patterns of antimicrobial resistance. METHODOLOGY:In this study we determined the frequency and antimicrobial suscep...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2604
更新日期:2013-04-17 00:00:00
abstract:INTRODUCTION:Miscarriage is one of the most common adverse pregnancy outcomes. The aim of this study was to investigate the relationship between miscarriage in humans and infections caused by zoonotic bacteria and genital pathogens. METHODOLOGY:Cervicovaginal swabs and placenta samples from 132 women with miscarriage ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9829
更新日期:2019-05-31 00:00:00
abstract:INTRODUCTION:Due to intercontinental traffic, population migration trends, natural disasters, and climate change, imported malaria remains important to consider in a febrile returning traveler. This study aims to raise awareness about malaria and help European clinicians maintain a working knowledge of this disease by ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6799
更新日期:2016-08-02 00:00:00
abstract:INTRODUCTION:Nosocomial infections are normally hospital acquired. Nasal carriage of Staphylococcus aureus (S. aureus) is very common and may be transmitted via a hand-to-nose route. The objective of the present study was to screen healthcare workers for the colonization of their nasal cavities with MRSA. METHODOLOGY:...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.3589
更新日期:2014-03-13 00:00:00
abstract:INTRODUCTION:Cholera still remains a significant cause of morbidity and mortality in developing countries, although comprehensive surveillance data to inform policy and strategies are scarce. METHODOLOGY:A desk review of the national cholera database and zonal and districts reports was conducted. Interviews were condu...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.3506
更新日期:2014-06-11 00:00:00
abstract:INTRODUCTION:A country's development level is measured with a quantitative parameter called the human development index (HDI). The present study researched the effects of HDI parameters (such as healthcare standards, income, and education level) on the incidence of tuberculosis. METHODOLOGY:HDI data of 36 provinces of...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.8101
更新日期:2016-11-24 00:00:00
abstract:INTRODUCTION:There is an urgent need for affordable point-of-care diagnostics for the differentiation of febrile illnesses and the confirmation of typhoid in endemic countries. METHODOLOGY:Blood samples were collected from febrile patients with clinical suspicion of typhoid and screened for typhoid fever using the Wid...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1836
更新日期:2011-07-27 00:00:00
abstract::Zika virus (ZIKV) infection is spreading rapidly within the Americas after originating from an outbreak in Brazil. We describe the current ZIKV infection epidemic in Brazil and the neurological symptoms arising. First cases of an acute exanthematic disease were reported in Brazil's Northeast region at the end of 2014....
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.8217
更新日期:2016-02-28 00:00:00
abstract:INTRODUCTION:Diagnosis of bloodstream infections using bacteriological cultures suffers from low sensitivity and reporting delay. Advanced molecular techniques introduced in many laboratories provide rapid results and may show improvements in patient outcomes. This study aimed to evaluate the usefulness of a molecular ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.4867
更新日期:2014-10-15 00:00:00
abstract::Lebanon yearly witnesses a high flux of expatriates and workers from Dengue virus (DENV) endemic regions. Multiple cases of Dengue fever have been documented at the American University of Beirut Medical Center (AUBMC) in travellers to endemic regions. Given the presence of the Aedes aegypti mosquito in Lebanon, introd...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9954
更新日期:2018-04-30 00:00:00
abstract:INTRODUCTION:The emergence and rapid spread of blaIMP and blaVIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options. METHODOLOGY:A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudomonas aeruginosa (61),...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2268
更新日期:2012-11-26 00:00:00
abstract:INTRODUCTION:When a surgical operation is to be conducted through a hair bearing part of the body, hair removal is often performed. This study aimed to evaluate the relationship of two methods of preoperative hair removal to postoperative wound infection in a developing country where razor shaving is very popular. MET...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章,随机对照试验
doi:10.3855/jidc.1527
更新日期:2011-10-13 00:00:00
abstract:INTRODUCTION:Melioidosis can have multiple organ involvement which can then mimic other infections. The aim of this study was to determine if there are any factors significantly associated with melioidosis which can inform diagnostic evaluations before receiving the results of confirming laboratory testing. METHODOLOG...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10727
更新日期:2019-02-28 00:00:00
abstract::Guangxi is a province of China endemic for enteric fever. Salmonella enterica serovar Paratyphi A has been found to be causing more and more enteric fever episodes in the last 10 years, evident not only from routine surveillance but also from prospective population-based surveillance. The prevalent pattern of paratyph...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.223
更新日期:2008-08-30 00:00:00
abstract::Brucellosis is an emergent and endemic zoonotic disease in Bosnia and Herzegovina. In this report we have diagnosed the first case of human brucellosis in Bosnia and Herzegovina, using molecular and microbiological tests, caused by live attenuated Brucella melitensis Rev.1 strain. The infection was caused through unin...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.11949
更新日期:2020-02-29 00:00:00
abstract:BACKGROUND:Non-typhoidal Salmonella are frequently food-borne zoonotic pathogens that may cause invasive disease in HIV-positive individuals. METHODOLOGY:Invasive isolates (n = 652) of Salmonella Typhimurium from human patients in Gauteng Province of South Africa were investigated for the years 2006 and 2007. Bacteria...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.549
更新日期:2009-09-15 00:00:00
abstract:INTRODUCTION:Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in the region of the city B...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.12552
更新日期:2020-06-30 00:00:00
abstract:INTRODUCTION:The aim of this study was to assess tuberculosis (TB) knowledge, attitudes, and practices in both the general population and risk groups in Thailand. METHODOLOGY:In a cross-sectional survey, a general population (n = 3,074) and family members of a TB patient (n = 559) were randomly selected, using stratif...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6963
更新日期:2016-08-02 00:00:00
abstract:INTRODUCTION:The goal of this study was to evaluate whether any characteristics that are evident at presentation for urgent medical attention could be used to differentiate cases of H5N1 in the absence of viral testing. METHODOLOGY:Information about exposure to poultry, clinical signs and symptoms, treatments, and out...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.3329
更新日期:2014-02-13 00:00:00
abstract:INTRODUCTION:Although omphalitis (umbilical infections) among newborns is common and a major cause of neonatal deaths in developing countries, information on its burden and etiology from community settings is lacking. This study aimed to determine the incidence and etiology of omphalitis in newborns in high neonatal mo...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章,评审
doi:10.3855/jidc.1229
更新日期:2011-12-13 00:00:00
abstract::Zika virus (ZIKV) is an emerging arbovirus of the Flaviviridae family and is related to dengue, Chikungunya, West Nile, yellow fever, and Japanese encephalitis viruses. ZIKV was first discovered in Uganda in 1947. Different species of mosquito from the Aedes genus, mainly A. aegypti and A. albopictus are the vectors r...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章,评审
doi:10.3855/jidc.8350
更新日期:2016-03-31 00:00:00
abstract:INTRODUCTION:The impact of mutations in the reverse transcriptase region of HBV on serum HBsAg titer and its correlation with HBV DNA is largely unknown. METHODOLOGY:A total of 644 patients, with a history of lamivudine or adefovir dipivoxil resistance who underwent genotypic resistance tests, were enrolled in this st...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.11447
更新日期:2019-11-30 00:00:00
abstract:INTRODUCTION:Brucellosis is a multisystemic infectious disease, which can manifest as endocarditis. Diagnosis can be challenging. CASE PRESENTATION:An 80-year-old male patient presented with fever 38.5 °C, cough and progressive shortness of breath of 14 days. History of Brucellosis 18 months earlier inadequately treat...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10186
更新日期:2018-02-22 00:00:00
abstract:INTRODUCTION:When bacterial pericarditis is suspected, urgent pericardial drainage combined with intravenous antibacterial therapy is mandatory to avert devastating, life-threatening complications. There have been scanty results on antimicrobial susceptibility of common causative microorganisms of bacterial pericarditi...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6027
更新日期:2015-07-30 00:00:00
abstract:INTRODUCTION:Assays based on DNA amplification can provide information that contributes to the initial management of patients with leptospirosis. However, these have not been adopted in Uruguay. Our aim was to evaluate the performance of the lipL32 real-time PCR (qPCR) for diagnosis of leptospirosis. METHODOLOGY:We an...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.4110
更新日期:2013-12-15 00:00:00
abstract:INTRODUCTION:Urinary Tract Infections (UTIs) is one of the most common infections worldwide. UTIs remain a challenge to the healthcare system because of the emergence of antimicrobial resistance. The aim of this study is to report the most common UTI-causative organisms associated with the emergence of antimicrobial re...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9517
更新日期:2018-04-30 00:00:00
abstract:INTRODUCTION:Antimicrobial resistance (AR) is a multifaceted problem of global significance. In addition to developing new drugs and using antimicrobial guidelines, it is essential that health professionals understand all aspects of the problem and the most effective ways to handle it. This study evaluated pharmacists'...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.4853
更新日期:2015-03-15 00:00:00
abstract:INTRODUCTION:Saudi Arabia (SA) experienced a highly pathogenic avian influenza (HPAI) H5N1 outbreak in domesticated birds in 2007. METHODOLOGY:Forty-three hemagglutinin (HA) and 41 neuraminidase (NA) genes of HPAI H5N1 viruses were sequenced and phylogenetic analyses of completely sequenced genes were performed to com...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6546
更新日期:2015-11-30 00:00:00
abstract:INTRODUCTION:Salmonella infections are increasing worldwide, but there are few reports on Salmonella surveillance in African countries and other developing countries. This has made it difficult to estimate the actual burden of salmonellosis, especially in Africa. This study was conducted in a neglected Northern Region ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.3548
更新日期:2013-10-15 00:00:00
abstract:INTRODUCTION:Invasive fungal infection (IFI) is a major cause of morbidity and mortality in allogeneic hematopoietic stem cell transplantation (allo-HSCT) recipients. A previous history of IFI is not an absolute contraindication for allo-HSCT, particularly in the era of secondary antifungal prophylaxis (SAP). Prompt di...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9961
更新日期:2018-09-30 00:00:00