Abstract:
INTRODUCTION:Assays based on DNA amplification can provide information that contributes to the initial management of patients with leptospirosis. However, these have not been adopted in Uruguay. Our aim was to evaluate the performance of the lipL32 real-time PCR (qPCR) for diagnosis of leptospirosis. METHODOLOGY:We analyzed by microscopic agglutination test (MAT) and lipL32 qPCR serum samples from 183 patients with suspected leptospirosis. To establish the analytical sensitivity of the qPCR, experimentally spiked samples with known amounts of Leptospira interrogans were analyzed. RESULTS:The analytical sensitivity of the qPCR was 102 leptospires/mL. In 98 patients MAT results were negative meanwhile 85 showed positive reactions, revealing acute infections. Twenty six acute-phase sera of these 85 patients showed a positive signal by qPCR (diagnostic sensitivity 30%). In these patients the average time between onset of symptoms and collection of the first sample was 8 days. In patients with negative results for qPCR and positive MAT results (n=59) the average interval between onset of symptoms and collection of the first sample was 13 days. The qPCR did not yield false positive results. CONCLUSIONS:The qPCR had a lower diagnostic sensitivity than MAT and a higher cost. However, it allowed to make an early diagnosis in 26 patients. In patients with confirmed acute infections and negative results by qPCR, more than 8 days had elapsed between the onset of the illness and extraction of the first serum sample. Our data support that the qPCR from sera have clinical utility within the first week of illness.
journal_name
J Infect Dev Ctriesjournal_title
Journal of infection in developing countriesauthors
González S,Geymonat JP,Hernández E,Marqués JM,Schelotto F,Varela Gdoi
10.3855/jidc.4110subject
Has Abstractpub_date
2013-12-15 00:00:00pages
941-5issue
12eissn
2036-6590issn
1972-2680journal_volume
7pub_type
杂志文章abstract:INTRODUCTION:Evaluating the attenuation of influenza viruses in animal studies is important in developing safe and effective vaccines. This study aimed to demonstrate that the microRNA (miRNA)-let-7b-mediated attenuated influenza viruses (miRT-H1N1) are sufficiently attenuated and safe in mice. METHODOLOGY:The pathoge...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6861
更新日期:2016-09-30 00:00:00
abstract:INTRODUCTION:Giardiasis is a human health concern worldwide, especially among schoolchildren. Giardia duodenalis genotypes A and B are infective to humans, but their zoonotic potential remains controversial. In Mexico, the most prevalent genotype is A, but B was also detected in southeastern Mexico. In Sinaloa state, n...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.8223
更新日期:2017-02-28 00:00:00
abstract:INTRODUCTION:Leptospirosis is a zoonotic disease caused by the spirochete of genus Leptospira with widespread distribution in tropical, subtropical and temperate zones. Leptospirosis is often confused with other febrile illnesses including jaundice, dengue, and malaria. Generally, the disease is often underdiagnosed or...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9545
更新日期:2018-01-31 00:00:00
abstract:INTRODUCTION:Antimicrobial substances (AMS) produced by bacteria may reduce or prevent the growth of pathogenic and spoilage microorganisms in food. In this study, 16 isolates of Acinetobacter baumannii/calcoaceticus (ABC) complex, previously obtained from reconstituted infant milk formula (IMF) samples and the prepara...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9894
更新日期:2018-05-31 00:00:00
abstract:INTRODUCTION:Rotavirus (RV) is the leading cause of severe diarrhea-associated morbidity and mortality among children worldwide. Limited data exist on the epidemiology and burden of rotavirus gastroenteritis in Armenia. The purpose of this study is to estimate the economic losses and describe the epidemiological charac...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10883
更新日期:2019-04-30 00:00:00
abstract:INTRODUCTION:The novel coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the coronavirus disease 2019 (COVID-19). First COVID-19 case was detected in March, 10, 2020 in Turkey and as of May, 18, 2020 148,067 cases have been identified and 4096 citizens have died. Tuberculosis (...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.13152
更新日期:2020-07-31 00:00:00
abstract:INTRODUCTION:HIV causes progressive impairment of the cellular immune system leading to increased susceptibility to infectious agents. Parasitic infestations are common in HIV-infected patients and usually lead to diarrhoea. Few studies have addressed the issue of intestinal parasites among HIV-infected persons in Came...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2757
更新日期:2013-02-15 00:00:00
abstract::Splenic abscess due to Brucella spp. is extremely rare. We report a case of a splenic abscess due to Brucella spp. in a 61-year-old male patient. Brucella slide and tube agglutination tests (Wright) were positive while blood culture and culture from splenic tissue yielded negative results. The abdominal ultrasonograph...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.204
更新日期:2008-10-01 00:00:00
abstract:INTRODUCTION:Methicillin resistant Staphylococcus aureus (MRSA) causes illness to people and can be picked up from both healthcare facilities and the environment leading to high morbidity and mortality. The study was aimed at identifying phenotypic characteristics of Methicillin-Resistant Staphylococcus aureus and dete...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.12174
更新日期:2020-06-30 00:00:00
abstract:BACKGROUND:This study determines the prevalence and antibiotic resistance of Salmonella serovars from humans and chickens in Ibadan, Nigeria, in 2004-2007. METHODOLOGY:A total of 991 blood samples were collected from patients in 2004 to 2005 and 641 fecal samples were collected from poultry farms in 2007. All Salmonel...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.909
更新日期:2010-09-03 00:00:00
abstract::We present the case of a 42-year-old male patient who applied to the emergency department of our hospital with clinical nephritis, orchitis, acute renal failure without endocarditis, and a low-grade fever. Brucella agglutinin titers were 1:160, Rose Bengal test was positive and Brucella melitensis was isolated from ur...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1442
更新日期:2011-12-13 00:00:00
abstract:INTRODUCTION:Imported parasitosis, which do not require an invertebrate vector, are extremely dangerous and can lead to the occurrence of disease in currently parasite free areas. In the present study we report a case of multi-parasitic infection in a young immigrant from Ghana to Italy caused by filaria, Schistosoma s...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.13151
更新日期:2020-11-30 00:00:00
abstract:BACKGROUND:In the year 2005, an epidemic of Japanese encephalitis (JE) occurred in the northern states of India. The present study was planned to reconfirm the circulation of JE in the area and to assess the trend of the disease to slow down the burden of JE. METHODOLOGY:Surveillance was conducted to identify patients...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.470
更新日期:2009-08-30 00:00:00
abstract:INTRODUCTION:Sexually transmitted infections (STI) are a major public health, social, and economic problem leading to morbidity, mortality, and stigma. This study was conducted to determine the prevalence of STIs, investigate behavioral risk factors and the relationship between the STIs/HIV and demographic factors. ME...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.5964
更新日期:2015-04-15 00:00:00
abstract:INTRODUCTION:In August 2011, Chittoor district authorities reported a cluster of suspected human anthrax cases to the Andhra Pradesh state surveillance unit. We investigated this cluster to confirm its etiology, describe its magnitude, identify potential risk factors, and make recommendations for preventing similar out...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2635
更新日期:2012-10-19 00:00:00
abstract:INTRODUCTION:Hypodermosis is an ectoparasitic disease of cattle caused by Hypoderma lineatum and Hypoderma bovis. It is an important health problem of cattle, leading to considerable economic losses. There are various factors that are involved in the spread of this disease such as herd size, location, temperature, humi...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.5387
更新日期:2015-08-29 00:00:00
abstract:INTRODUCTION:Although the government of Yemen changed the national policy for treating malaria in November 2005 from chloroquine to combination drugs in the form of artesunate + sulphadoxine-pyrimethamine (SP) as first line and lumefantrine + artemether as second line treatment for uncomplicated malaria, clinicians in ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2528
更新日期:2013-05-13 00:00:00
abstract:INTRODUCTION:Infants represent an important risk group for influenza associated hospitalizations and mortality. This study evaluated the clinical presentations, hospitalization course and outcome of infants hospitalized with the pandemic influenza A H1N1 [Influenza A(H1N1)pdm09] in relation to their previous health sta...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2339
更新日期:2012-08-21 00:00:00
abstract:INTRODUCTION:Women infected with human immunodeficiency virus (HIV) have a higher risk of contracting human papillomavirus (HPV) infections and are more prone to develop cervical cancer. The objective of this study was to determine the prevalence of HPV and its association with risk factors among Moroccan women living ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9711
更新日期:2018-06-30 00:00:00
abstract::The increasing prevalence of foodborne diseases observed in developing countries has been linked to a rise in the consumption of raw foods. However, unlike the classical pathogens that are commonly implicated in foodborne illnesses, members of the genus Acinetobacter are rarely associated with diarrheal disease, proba...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章,评审
doi:10.3855/jidc.8418
更新日期:2017-02-28 00:00:00
abstract:INTRODUCTION:Norovirus is not usually investigated in diarrheal patients in Bangladesh which may account for the many cases where no pathogens are identified. METHODOLOGY:Stool specimens collected from diarrheal patients from three hospitals in Bangladesh during 2011 were investigated for norovirus RNA using real-time...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2944
更新日期:2013-11-15 00:00:00
abstract:INTRODUCTION:Group A streptococci (GAS) is responsible of several human diseases ranging from mild infection to severe invasive toxin-mediated disease and post-infectious sequelae. Accordingly, a GAS surveillance program based on molecular techniques is advisable for its epidemiological control. Pulsed-field gel electr...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6115
更新日期:2015-07-30 00:00:00
abstract:BACKGROUND:Burkholderia cepacia has the potential to cause fatal infections in ICUs, and multidrug resistance makes them a serious threat in hospital settings. The aim of this study was to evaluate the epidemiology of B. cepacia infections in our hospital. METHODOLOGY:The incidence, clinical characteristics, antimicro...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.124
更新日期:2009-05-01 00:00:00
abstract:INTRODUCTION:Urinary Tract Infections (UTIs) is one of the most common infections worldwide. UTIs remain a challenge to the healthcare system because of the emergence of antimicrobial resistance. The aim of this study is to report the most common UTI-causative organisms associated with the emergence of antimicrobial re...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9517
更新日期:2018-04-30 00:00:00
abstract:INTRODUCTION:Infections caused by multidrug-resistant bacteria are increasingly common and represent a serious problem for public health. Staphylococcus aureus is one of the major agents of infections, and methicillin-resistant S. aureus (MRSA) has spread worldwide. The aim of this study was to phenotypically and genot...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.7887
更新日期:2016-12-30 00:00:00
abstract:INTRODUCTION:We assessed the influence of a result-based financing (RBF) model, which included incentives for Primary Healthcare facilities on TB treatment outcomes. METHODOLOGY:We compared TB patients > 17 years and their treatment outcomes among those who did and did not benefit from RBF-model in 14 districts of Ode...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.11292
更新日期:2019-07-31 00:00:00
abstract:INTRODUCTION:Brucellosis is a multisystemic infectious disease, which can manifest as endocarditis. Diagnosis can be challenging. CASE PRESENTATION:An 80-year-old male patient presented with fever 38.5 °C, cough and progressive shortness of breath of 14 days. History of Brucellosis 18 months earlier inadequately treat...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10186
更新日期:2018-02-22 00:00:00
abstract:INTRODUCTION:Fungi of the genus Cryptococcus are cosmopolitan and may be agents of opportunistic mycoses in immunocompromised and sometimes immunocompetent individuals. Cryptococcus species are frequently isolated from trees and bird excreta in the environment and infection occurs by inhalation of propagules dispersed ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10849
更新日期:2019-06-30 00:00:00
abstract::Pegylated interferon (PEG-IFN) alpha and ribavirin therapy has become the standard treatment in chronic hepatitis C virus (HCH)-infected patients. While thrombocytopenia associated with IFN use is frequently observed among these patients, autoimmune thrombocytopenia is one of the rarely observed adverse effects. In th...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.559
更新日期:2009-09-15 00:00:00
abstract:INTRODUCTION:Dentistry is predominantly a field of surgery, involving exposure to blood and other potentially infectious materials and therefore requires a high standard of infection control and safety practice in controlling cross-contamination and occupational exposures to blood- and saliva-borne diseases. METHODOLO...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1652
更新日期:2011-11-15 00:00:00