Abstract:
INTRODUCTION:Group A streptococci (GAS) is responsible of several human diseases ranging from mild infection to severe invasive toxin-mediated disease and post-infectious sequelae. Accordingly, a GAS surveillance program based on molecular techniques is advisable for its epidemiological control. Pulsed-field gel electrophoresis (PFGE) is the gold standard for GAS molecular subtyping, but a major disadvantage is the length of the procedure, which takes 1-3 days of work, minimum. The aim of this study was to develop a rapid and cost-effective procedure for PFGE subtyping of GAS isolates. METHODOLOGY:Different incubation times of GAS, immobilized in agarose miniplugs, in solutions containing lysozyme and/or mutanolysine followed by solutions with urea instead of proteinase K, were assayed. DNA was restricted with SmaI and the fingerprints were obtained in clamped homogeneous electric field (CHEF) chambers and minichambers. The modified procedure was used to subtype 22 GAS isolates. RESULTS:Intact DNA molecules of GAS immobilized in agarose miniplugs were prepared incubating the cells, in situ, with a solution containing lysozyme for 4 hours, followed by the incubation in a non-enzymatic solution with urea for 2 hours. SmaIDNA macrorestriction fragments were well resolved in 5 hours and 14 minutes by electrophoresis in a CHEF minichamber at 10 V/cm. This procedure for GAS DNA preparation was useful for fingerprinting GAS strains in the format of CHEF Mapper (BioRad). CONCLUSIONS:The procedure took 13 hours for GAS strains subtyping. Both sample preparation and electrophoresis in CHEF minichamber represent an economic alternative for performing massive epidemiological studies of this human pathogen.
journal_name
J Infect Dev Ctriesjournal_title
Journal of infection in developing countriesauthors
López Y,Manrique V,Niubo E,Valdés-Dapena M,Pérez Amarillo J,López I,Parra B,Escalona Venegas G,Riverón AM,López-Cánovas Ldoi
10.3855/jidc.6115subject
Has Abstractpub_date
2015-07-30 00:00:00pages
710-9issue
7eissn
2036-6590issn
1972-2680journal_volume
9pub_type
杂志文章abstract:INTRODUCTION:The sputum smear or the culture are the definitive diagnosis of pulmonary tuberculosis. Only a fraction of clinical patients are culture-confirmed. METHODOLOGY:A total of 24 clinical cases (40 ± 14 years old) with positive smear and negative co-morbidity were studied. Cases were selected from 600 patients...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10583
更新日期:2019-01-31 00:00:00
abstract:INTRODUCTION:In some developing countries, tuberculosis and brucellosis, which are commom causes of spinal infections, are still common infectious diseases. However, co-occurrence of spinal tuberculosis and brucellosis is rare. METHODOLOGY:We report a case a 47-year-old male engaged in aquaculture with a medical histo...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.12647
更新日期:2020-10-31 00:00:00
abstract:INTRODUCTION:Sexually transmitted infections (STI) are a major public health, social, and economic problem leading to morbidity, mortality, and stigma. This study was conducted to determine the prevalence of STIs, investigate behavioral risk factors and the relationship between the STIs/HIV and demographic factors. ME...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.5964
更新日期:2015-04-15 00:00:00
abstract:INTRODUCTION:Human co-infection with Plasmodium falciparum and helminthes is ubiquitous throughout Africa. This study aimed to determine the co-infections of Plasmodium falciparum infection in HIV and intestinal parasitic infections, and their immunological distribution, in Benin City, Nigeria. METHODOLOGY:A total of ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1889
更新日期:2012-05-14 00:00:00
abstract:BACKGROUND:Research on the effects of corticosteroids in bacterial meningitis (BM) yielded conflicting results. While some studies reveal that corticosteroids improve the outcomes in BM treatments, others provide strong evidence that patients do not profit from this treatment. We investigated the factors that may impac...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.51
更新日期:2009-12-14 00:00:00
abstract:INTRODUCTION:Evaluating the attenuation of influenza viruses in animal studies is important in developing safe and effective vaccines. This study aimed to demonstrate that the microRNA (miRNA)-let-7b-mediated attenuated influenza viruses (miRT-H1N1) are sufficiently attenuated and safe in mice. METHODOLOGY:The pathoge...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6861
更新日期:2016-09-30 00:00:00
abstract:BACKGROUND:Brucella is a facultative, intracellular pathogen that causes severe disease in animals and humans. Immunity against Brucella involves both humoral and cellular responses. To investigate the characteristics of immune response in acute brucellosis in Sprague-Dawley (SD) rats, IgG and its subclass specific imm...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.248
更新日期:2009-06-01 00:00:00
abstract:INTRODUCTION:Dengue virus (DENV) infection is currently a major cause of morbidity in the world, and its incidence has mainly increased during the last years in Latin America, including Paraguay. The objective of this study was to compare the clinical and laboratory findings of dengue and assess whether the serotype is...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.11584
更新日期:2019-12-31 00:00:00
abstract:INTRODUCTION:There is a paucity of information on the frequency of Chlamydia trachomatis-induced reactive arthritis (ReA) and undifferentiated spondyloarthropathy (uSpA) in India. In this study, arthritic patients suffering from ReA, uSpA, and rheumatoid arthritis (RA) were screened to investigate the presence of C. tr...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.3644
更新日期:2014-05-14 00:00:00
abstract:INTRODUCTION:The study aimed to investigate the resistance of methicillin resistant Staphylococcus aureus (MRSA), an indicator used in hospitals but isolated from faecal samples of children in the community, to commonly used antibiotics and antiseptic agents. METHODOLOGY:S. aureus isolates were identified by phenotypi...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1609
更新日期:2012-04-13 00:00:00
abstract:INTRODUCTION:Antimicrobial stewardship practices are crucial for the regular surveillance to change the antimicrobial policy. This study was conducted to decide the prevalence of common bacteria and their antibiogram regarding antimicrobial stewardship program within one year, at the regional and district, Stanger hosp...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10685
更新日期:2019-08-31 00:00:00
abstract:INTRODUCTION:Hand, foot, and mouth disease (HFMD) is a common childhood illness caused by enteroviruses. A passive surveillance system has been implemented in Shanghai Pudong since 2008 and etiology surveillance since 2009.We characterized the epidemiology and the etiology of HFMD in Pudong from 2008-2013. METHODOLOGY...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6118
更新日期:2016-06-30 00:00:00
abstract:INTRODUCTION:Hepatitis B infection is a serious global public health problem. The aim of the study was to assess the seroprevalance of hepatitis B surface antigen (HBsAg), as well as the risk factors associated with hepatitis B virus (HBV) infection among pregnant women attending antenatal care clinics of the Universit...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10018
更新日期:2018-10-31 00:00:00
abstract:INTRODUCTION:Acute respiratory infections (ARIs) are major causes of morbidity in early childhood. They are mainly caused by viruses, including influenza (INF) and respiratory syncytial viruses (RSV). We aimed to investigate the role of RSV and INF in children hospitalized for ARIs and to show the impact of RSV/INF rap...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.11386
更新日期:2019-05-16 00:00:00
abstract:INTRODUCTION:Mastitis is one of the most frequent and costly disease in cattle. We studied milk samples from cattle with mastitis from farms in Kosovo to identify mastitis-causing pathogens and possible effective antibiotics. Our ultimate goal is to help implement adequate antibiotic management and treatment practices ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.7912
更新日期:2016-10-31 00:00:00
abstract:INTRODUCTION:Dengue virus (DENV) is principally transmitted by the Aedes aegypti mosquito. To date, mosquito population control remains the key strategy for reducing the continuing spread of DENV. The focus on the development of new vector control strategies through an understanding of the mosquito-virus relationship i...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.6422
更新日期:2015-12-30 00:00:00
abstract::Lebanon yearly witnesses a high flux of expatriates and workers from Dengue virus (DENV) endemic regions. Multiple cases of Dengue fever have been documented at the American University of Beirut Medical Center (AUBMC) in travellers to endemic regions. Given the presence of the Aedes aegypti mosquito in Lebanon, introd...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9954
更新日期:2018-04-30 00:00:00
abstract:INTRODUCTION:Nosocomial pneumonia (NP) and ventilator associated pneumonia (VAP) occur most frequently in intensive care units (ICU). This study seeks to determine the etiological agents of NP and VAP along with their antibacterial susceptibility patterns, and to evaluate the factors contributing to patient mortality. ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.1212
更新日期:2011-04-26 00:00:00
abstract:INTRODUCTION:Inappropriate use of antibiotics is recognised as a leading cause of antibiotic resistance. Little is known about antibiotic prescribing practices at public health facilities in low- and middle-income countries. We examined patterns of antibiotic prescribing in public health facilities in Nepal and explore...
journal_title:Journal of infection in developing countries
pub_type: 社论,多中心研究
doi:10.3855/jidc.11817
更新日期:2020-01-31 00:00:00
abstract:INTRODUCTION:Urinary Tract Infections (UTIs) is one of the most common infections worldwide. UTIs remain a challenge to the healthcare system because of the emergence of antimicrobial resistance. The aim of this study is to report the most common UTI-causative organisms associated with the emergence of antimicrobial re...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9517
更新日期:2018-04-30 00:00:00
abstract:INTRODUCTION:Dogs may act as potential sources of zoonotic parasites, e.g. intestinal helminths like Toxocara spp., Ancylostoma spp., Echinococcus spp. In particular circumstances, the environment contaminated by parasitic elements represents a source of infection for people and animals. The present study has evaluated...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9865
更新日期:2018-08-31 00:00:00
abstract:INTRODUCTION:Antimicrobial substances (AMS) produced by bacteria may reduce or prevent the growth of pathogenic and spoilage microorganisms in food. In this study, 16 isolates of Acinetobacter baumannii/calcoaceticus (ABC) complex, previously obtained from reconstituted infant milk formula (IMF) samples and the prepara...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.9894
更新日期:2018-05-31 00:00:00
abstract:INTRODUCTION:Fungi of the genus Cryptococcus are cosmopolitan and may be agents of opportunistic mycoses in immunocompromised and sometimes immunocompetent individuals. Cryptococcus species are frequently isolated from trees and bird excreta in the environment and infection occurs by inhalation of propagules dispersed ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.10849
更新日期:2019-06-30 00:00:00
abstract::Chikungunya virus is an emerging arboviral disease that has been spreading rapidly across south Asia in recent years. Until recently, no chikungunya cases have been reported in Nepal. For the first time, we report three cases of chikungunya virus infection in Nepal. ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.3701
更新日期:2014-06-11 00:00:00
abstract::The incidence of invasive salmonellosis has increased among children and HIV-infected adults in Malawi. This has been associated with the emergence of drug resistance in the non-typhoidal Salmonella serovars Enteritidis and Typhimurium. In contrast, S. Typhi isolates have remained fully sensitive to commonly used anti...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章,评审
doi:10.3855/jidc.158
更新日期:2008-12-01 00:00:00
abstract:INTRODUCTION:Although the government of Yemen changed the national policy for treating malaria in November 2005 from chloroquine to combination drugs in the form of artesunate + sulphadoxine-pyrimethamine (SP) as first line and lumefantrine + artemether as second line treatment for uncomplicated malaria, clinicians in ...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2528
更新日期:2013-05-13 00:00:00
abstract:INTRODUCTION:Staphylococcus aureus-induced bloodstream infections (BSIs) remain a prevalent clinical challenge and the underlying pathogenesis is still poorly understood. The aim of this study was to investigate the inflammatory responses and histopathological changes in BSIs in mice. METHODOLOGY:Male C57BL/6 mice wer...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.7800
更新日期:2017-04-30 00:00:00
abstract:BACKGROUND:Tungiasis, caused by the sand flea Tunga penetrans, is highly prevalent in many resource-poor communities in sub-Saharan Africa, Latin America and the Caribbean. Studies on knowledge and treatment practices related to tungiasis in affected populations are virtually nonexistent. To fill this gap, we performed...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.418
更新日期:2009-07-01 00:00:00
abstract:INTRODUCTION:Helicobacter pylori cause damage to gastric epithelial cells and alterations in the p53 gene that lead to cancer development. This study aimed to determine the correlation of p53 expression with H. pylori using immunohistochemistry, RFLP-PCR, and histopathology. METHODOLOGY:Gastric biopsy samples from gas...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.2993
更新日期:2013-09-16 00:00:00
abstract:INTRODUCTION:Antimicrobial resistance (AR) is a multifaceted problem of global significance. In addition to developing new drugs and using antimicrobial guidelines, it is essential that health professionals understand all aspects of the problem and the most effective ways to handle it. This study evaluated pharmacists'...
journal_title:Journal of infection in developing countries
pub_type: 杂志文章
doi:10.3855/jidc.4853
更新日期:2015-03-15 00:00:00