EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice.


BACKGROUND:Glioblastoma multiforme (GBM) is the most lethal form of brain cancer. With the available treatments, survival does not exceed 12-14 mo from the time of diagnosis. We describe a novel strategy to selectively induce the death of glioblastoma cells and other cancer cells that over-express the EGF receptor. Using a non-viral delivery vector that homes to the EGF receptor, we target synthetic anti-proliferative dsRNA (polyinosine-cytosine [poly IC]), a strong activator of apoptosis, selectively to cancer cells. METHODS AND FINDINGS:Poly IC was delivered by means of a non-viral vector: 25kDa polyethylenimine-polyethyleneglycol-EGF (PEI25-PEG-EGF). EGFR-targeted poly IC induced rapid apoptosis in the target cells in vitro and in vivo. Expression of several cytokines and "bystander killing" of untransfected tumor cells was detected in vitro and in vivo. Intra-tumoral delivery of the EGFR-targeted poly IC induced the complete regression of pre-established intracranial tumors in nude mice, with no obvious adverse toxic effects on normal brain tissue. A year after treatment completion the treated mice remain cancer-free and healthy. Similarly, non-viral delivery of poly IC completely eliminated pre-established breast cancer and adenocarcinoma xenografts derived from EGFR over-expressing cancer cell lines, suggesting that the strategy is applicable to other EGFR-over-expressing tumors. CONCLUSION:The strategy described has yielded an effective treatment of EGFR over-expressing GBM in an animal model. If this strategy is translated successfully to the clinical setting, it may actually offer help to GBM patients. Moreover the elimination of two additional EGFR over-expressing cancers in vivo suggests that in principle this strategy can be applied to treat other tumors that over-express EGFR.


PLoS Med


PLoS medicine


Shir A,Ogris M,Wagner E,Levitzki A





Has Abstract


2006-01-01 00:00:00














  • Scaling up diarrhea prevention and treatment interventions: a Lives Saved Tool analysis.

    abstract:BACKGROUND:Diarrhea remains a leading cause of mortality among young children in low- and middle-income countries. Although the evidence for individual diarrhea prevention and treatment interventions is solid, the effect a comprehensive scale-up effort would have on diarrhea mortality has not been estimated. METHODS A...

    journal_title:PLoS medicine

    pub_type: 杂志文章


    authors: Fischer Walker CL,Friberg IK,Binkin N,Young M,Walker N,Fontaine O,Weissman E,Gupta A,Black RE

    更新日期:2011-03-01 00:00:00

  • Acute-phase serum amyloid A: an inflammatory adipokine and potential link between obesity and its metabolic complications.

    abstract:BACKGROUND:Obesity is associated with low-grade chronic inflammation, and serum markers of inflammation are independent risk factors for cardiovascular disease (CVD). However, the molecular and cellular mechanisms that link obesity to chronic inflammation and CVD are poorly understood. METHODS AND FINDINGS:Acute-phase...

    journal_title:PLoS medicine

    pub_type: 杂志文章


    authors: Yang RZ,Lee MJ,Hu H,Pollin TI,Ryan AS,Nicklas BJ,Snitker S,Horenstein RB,Hull K,Goldberg NH,Goldberg AP,Shuldiner AR,Fried SK,Gong DW

    更新日期:2006-06-01 00:00:00

  • Association of coincident self-reported mental health problems and alcohol intake with all-cause and cardiovascular disease mortality: A Norwegian pooled population analysis.

    abstract:BACKGROUND:The disease burden attributable to mental health problems and to excess or harmful alcohol use is considerable. Despite a strong relationship between these 2 important factors in population health, there are few studies quantifying the mortality risk associated with their co-occurrence in the general populat...

    journal_title:PLoS medicine

    pub_type: 杂志文章


    authors: Degerud E,Høiseth G,Mørland J,Ariansen I,Graff-Iversen S,Ystrom E,Zuccolo L,Næss Ø

    更新日期:2020-02-03 00:00:00

  • Revision rates after primary hip and knee replacement in England between 2003 and 2006.

    abstract:BACKGROUND:Hip and knee replacement are some of the most frequently performed surgical procedures in the world. Resurfacing of the hip and unicondylar knee replacement are increasingly being used. There is relatively little evidence on their performance. To study performance of joint replacement in England, we investig...

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    pub_type: 杂志文章


    authors: Sibanda N,Copley LP,Lewsey JD,Borroff M,Gregg P,MacGregor AJ,Pickford M,Porter M,Tucker K,van der Meulen JH,Steering Committee of the National Joint Registry (NJR) for England and Wales.

    更新日期:2008-09-30 00:00:00

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    abstract::[This corrects the article DOI: 10.1371/journal.pmed.1002781.]. ...

    journal_title:PLoS medicine

    pub_type: 已发布勘误


    authors: Penn-Nicholson A,Hraha T,Thompson EG,Sterling D,Mbandi SK,Wall KM,Fisher M,Suliman S,Shankar S,Hanekom WA,Janjic N,Hatherill M,Kaufmann SHE,Sutherland J,Walzl G,De Groote MA,Ochsner U,Zak DE,Scriba TJ,ACS and GC6–74

    更新日期:2019-07-18 00:00:00

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    journal_title:PLoS medicine

    pub_type: 杂志文章,meta分析


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    更新日期:2005-03-01 00:00:00

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    journal_title:PLoS medicine

    pub_type: 杂志文章


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    更新日期:2013-11-01 00:00:00

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    pub_type: 杂志文章,评审


    authors: Stockman LJ,Bellamy R,Garner P

    更新日期:2006-09-01 00:00:00

  • Validation of IMPROD biparametric MRI in men with clinically suspected prostate cancer: A prospective multi-institutional trial.

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    journal_title:PLoS medicine

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    pub_type: 杂志文章


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    更新日期:2021-01-06 00:00:00

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    pub_type: 杂志文章


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    更新日期:2017-06-15 00:00:00

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    pub_type: 杂志文章


    authors: Leung W,Kvizhinadze G,Nair N,Blakely T

    更新日期:2016-08-09 00:00:00

  • IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study.

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    pub_type: 杂志文章


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    更新日期:2016-12-20 00:00:00

  • Major radiodiagnostic imaging in pregnancy and the risk of childhood malignancy: a population-based cohort study in Ontario.

    abstract:BACKGROUND:The association between fetal exposure to major radiodiagnostic testing in pregnancy-computed tomography (CT) and radionuclide imaging-and the risk of childhood cancer is not established. METHODS AND FINDINGS:We completed a population-based study of 1.8 million maternal-child pairs in the province of Ontari...

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    pub_type: 杂志文章


    authors: Ray JG,Schull MJ,Urquia ML,You JJ,Guttmann A,Vermeulen MJ

    更新日期:2010-09-07 00:00:00

  • Obstructive sleep apnea and risk of cardiovascular events and all-cause mortality: a decade-long historical cohort study.

    abstract:BACKGROUND:Obstructive sleep apnea (OSA) has been reported to be a risk factor for cardiovascular (CV) disease. Although the apnea-hypopnea index (AHI) is the most commonly used measure of OSA, other less well studied OSA-related variables may be more pathophysiologically relevant and offer better prediction. The objec...

    journal_title:PLoS medicine

    pub_type: 杂志文章


    authors: Kendzerska T,Gershon AS,Hawker G,Leung RS,Tomlinson G

    更新日期:2014-02-04 00:00:00

  • Correction: association between cutaneous nevi and breast cancer in the nurses' health study: a prospective cohort study.

    abstract::[This corrects the article DOI: 10.1371/journal.pmed.1001659.]. ...

    journal_title:PLoS medicine

    pub_type: 已发布勘误


    authors: PLOS Medicine Staff.

    更新日期:2015-03-03 00:00:00

  • Migration and first-year maternal mortality among HIV-positive postpartum women: A population-based longitudinal study in rural South Africa.

    abstract:BACKGROUND:In South Africa, within-country migration is common. Mobility affects many of the factors in the pathway for entry to or retention in care among people living with HIV. We characterized the patterns of migration (i.e., change in residency) among peripartum women from rural South Africa and their association ...

    journal_title:PLoS medicine

    pub_type: 杂志文章


    authors: Kim HY,Dobra A,Tanser F

    更新日期:2020-03-31 00:00:00

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    pub_type: 杂志文章


    authors: Rees CA,Pica N,Monuteaux MC,Bourgeois FT

    更新日期:2019-11-21 00:00:00

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    journal_title:PLoS medicine

    pub_type: 评论,杂志文章


    authors: Ford N,Hirnschall G

    更新日期:2013-10-01 00:00:00

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    更新日期:2017-06-27 00:00:00

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    pub_type: 已发布勘误


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    更新日期:2020-12-29 00:00:00

  • Decreased bone mineral density in adults born with very low birth weight: a cohort study.

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    journal_title:PLoS medicine

    pub_type: 杂志文章


    authors: Hovi P,Andersson S,Järvenpää AL,Eriksson JG,Strang-Karlsson S,Kajantie E,Mäkitie O

    更新日期:2009-08-01 00:00:00

  • Correction: A new aging measure captures morbidity and mortality risk across diverse subpopulations from NHANES IV: A cohort study.

    abstract::[This corrects the article DOI: 10.1371/journal.pmed.1002718.]. ...

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    更新日期:2011-03-01 00:00:00

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    pub_type: 杂志文章


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    更新日期:2019-05-03 00:00:00

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    journal_title:PLoS medicine

    pub_type: 临床试验,杂志文章


    authors: Druilhe P,Spertini F,Soesoe D,Corradin G,Mejia P,Singh S,Audran R,Bouzidi A,Oeuvray C,Roussilhon C

    更新日期:2005-11-01 00:00:00