Abstract：
:Orf virus (OV) is a zoonotic parapoxvirus that causes highly proliferative skin lesions which resolve with minimal inflammation and scarring. OV encodes two immunomodulators, vascular endothelial growth factor (VEGF)-E and interleukin-10 (ovIL-10), which individually modulate skin repair and inflammation. This study examined the effects of the VEGF-E and ovIL-10 combination on healing processes in a murine wound model. Treatments with viral proteins, individually and in combination, were compared to a mammalian VEGF-A and IL-10 combination. Wound biopsies were harvested to measure re-epithelialisation and scarring (histology), inflammation, fibrosis and angiogenesis (immunofluorescence), and gene expression (quantitative polymerase chain reaction). VEGF-E and ovIL-10 showed additive effects on wound closure and re-epithelialisation, and suppressed M1 macrophage and myofibroblast infiltration, while allowing M2 macrophage recruitment. The viral combination also increased endothelial cell density and pericyte coverage, and improved collagen deposition while reducing the scar area. The mammalian combination showed equivalent effects on wound closure, re-epithelialisation and fibrosis, but did not promote blood vessel stabilisation or collagen remodeling. The combination treatments also differentially altered the expression of transforming growth factor beta isoforms, Tgfβ1 and Tgfβ3. These findings show that the OV proteins synergistically enhance skin repair, and act in a complimentary fashion to improve scar quality.
journal_name
J Clin Medjournal_title
Journal of clinical medicineauthors
Wise LM,Stuart GS,Jones NC,Fleming SB,Mercer AAdoi
10.3390/jcm9041085subject
Has Abstractpub_date
2020-04-11 00:00:00issue
4issn
2077-0383pii
jcm9041085journal_volume
9pub_type
杂志文章abstract：BACKGROUND:The long-term stability after soft tissue graft for covering gingival recession remains a pivotal goal for both patient and periodontist. Therefore, the aim of this study was to compare the four-year outcomes of the coronally advanced flap (CAF) versus the pouch/tunnel (POT) technique, both combined with con...
journal_title：Journal of clinical medicine
pub_type： 杂志文章
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更新日期：2020-08-14 00:00:00
abstract：:Immune checkpoint inhibitors (ICI) have revolutionised cancer therapy. However, they have been effective in only a small subset of patients and a principal mechanism underlying immune-refractoriness is a 'cold' tumour microenvironment, that is, lack of a T-cell-rich, spontaneously inflamed phenotype. As such, there is...
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abstract：:Patients with chronic obstructive pulmonary disease (COPD), asthma and interstitial lung diseases (ILD) frequently suffer from cardiovascular comorbidities (CVC). Exercise training is a cornerstone intervention for the management of these conditions, however recommendations on tailoring programmes to patients sufferin...
journal_title：Journal of clinical medicine
pub_type： 杂志文章,评审
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更新日期：2019-09-13 00:00:00
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更新日期：2020-10-03 00:00:00
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更新日期：2019-12-28 00:00:00
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更新日期：2017-09-04 00:00:00
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更新日期：2020-06-05 00:00:00
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pub_type： 杂志文章
doi：10.3390/jcm9092772
更新日期：2020-08-27 00:00:00
abstract：BACKGROUND:In-depth knowledge about potential predictors of mortality in transcatheter aortic valve replacement (TAVR) is still warranted. Currently used risk stratification models for TAVR often fail to reach a holistic approach. We, therefore, aimed to create a new staged risk model for 1-year mortality including sev...
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doi：10.3390/jcm8101642
更新日期：2019-10-08 00:00:00
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journal_title：Journal of clinical medicine
pub_type： 杂志文章
doi：10.3390/jcm8122058
更新日期：2019-11-22 00:00:00
abstract：:The aim of this study was to evaluate the ability of a PIPS (photon-induced photoacoustic streaming) Er:YAG laser to reduce the root canal system bacterial count in vivo in comparison to the traditional irrigation technique. The post-operative patients' quality of life (QoL) after endodontic therapy was evaluated thro...
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pub_type： 杂志文章
doi：10.3390/jcm9123915
更新日期：2020-12-02 00:00:00
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pub_type： 杂志文章
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更新日期：2020-05-13 00:00:00
abstract：:(1) Background: The use of smartphone-based ecological momentary assessment (EMA) questionnaires in affective disorder research has rapidly increased. Though, a thorough understanding of experienced burden of and adherence to EMA is crucial in determining the usefulness of EMA. (2) Methods: Persons with current affect...
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更新日期：2020-01-23 00:00:00
abstract：INTRODUCTION:Arterial hypertension is the most frequent cause for spontaneous intracerebral hemorrhage (sICH) and may also cause left ventricular hypertrophy (LVH). We sought to analyze whether hypertensive sICH etiology is associated with LVH. METHODS:We analyzed consecutive patients with sICH who were admitted to ou...
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pub_type： 杂志文章
doi：10.3390/jcm9072148
更新日期：2020-07-08 00:00:00
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pub_type： 杂志文章
doi：10.3390/jcm8101526
更新日期：2019-09-23 00:00:00
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更新日期：2015-05-21 00:00:00
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更新日期：2020-08-28 00:00:00
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pub_type： 杂志文章,评审
doi：10.3390/jcm8071033
更新日期：2019-07-14 00:00:00
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更新日期：2020-05-26 00:00:00
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更新日期：2018-07-22 00:00:00
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更新日期：2020-11-07 00:00:00
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pub_type： 杂志文章
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更新日期：2020-03-16 00:00:00
abstract：:There are differences in the incidence, clinical presentation, molecular pathogenesis, and outcome of colorectal cancer (CRC) based on tumor location. Emerging research suggests that the perioperative carcinoembryonic antigen (CEA) ratio (post-op/pre-op CEA) is a prognostic factor for CRC patients. We aimed to determi...
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abstract：:Descriptive and retrospective studies without control groups have suggested a possible association between primary Sjögren's syndrome (pSS) and vitamin B12 (B12) deficiency. This is of importance because several mucosal and neurological features are common to these two conditions and could be prevented or reversed in ...
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abstract：:The main goal of the present study was the identification of cellular phenotypes in attention-deficit-/hyperactivity disorder (ADHD) patient-derived cellular models from carriers of rare copy number variants (CNVs) in the PARK2 locus that have been previously associated with ADHD. Human-derived fibroblasts (HDF) were ...
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