Abstract:
BACKGROUND:Dengue is a serious public health problem worldwide, including in Selangor, Malaysia. Being an important vector of dengue virus, Aedes aegypti are subjected to control measures which rely heavily on the usage of insecticides. Evidently, insecticide resistance in Ae. aegypti, which arise from several different point mutations within the voltage-gated sodium channel genes, has been documented in many countries. Thus, this robust study was conducted in all nine districts of Selangor to understand the mechanisms of resistance to various insecticides in Ae. aegypti. Mosquitoes were collected from dengue epidemic and non-dengue outbreak areas in Selangor. METHODS:Using the Center for Disease Control and Prevention (CDC) bottle assays, the insecticide resistance status of nine different Ae. aegypti strains from Selangor was accessed. Synergism tests and biochemical assays were conducted to further understand the metabolic mechanisms of insecticide resistance. Polymerase chain reaction (PCR) amplification and sequencing of the IIP-IIS6 as well as IIIS4-IIIS6 regions of the sodium channel gene were performed to enable comparisons between susceptible and resistant mosquito strains. Additionally, genomic DNA was used for allele-specific PCR (AS-PCR) genotyping of the gene to detect the presence of F1534C, V1016G and S989P mutations. RESULTS:Adult female Ae. aegypti from various locations were susceptible to malathion and propoxur. However, they exhibited different levels of resistance against dichlorodiphenyltrichloroethane (DDT) and pyrethroids. The results of synergism tests and biochemical assays indicated that the mixed functions of oxidases and glutathione S-transferases contributed to the DDT and pyrethroid resistance observed in the present study. Besides detecting three single kdr mutations, namely F1534C, V1016G and S989P, co-occurrence of homozygous V1016G/S989P (double allele) and F1534C/V1016G/S989P (triple allele) mutations were also found in Ae. aegypti. As per the results, the three kdr mutations had positive correlations with the expressions of resistance to DDT and pyrethroids. CONCLUSIONS:In view of the above outcomes, it is important to seek new tools for vector management instead of merely relying on insecticides. If the latter must be used, regular monitoring of insecticide resistance should also be carried out at all dengue epidemic areas. Since the eggs of Ae. aegypti can be easily transferred from one location to another, it is probable that insecticide-resistant Ae. aegypti can be found at non-dengue outbreak sites as well.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Leong CS,Vythilingam I,Liew JW,Wong ML,Wan-Yusoff WS,Lau YLdoi
10.1186/s13071-019-3472-1subject
Has Abstractpub_date
2019-05-16 00:00:00pages
236issue
1issn
1756-3305pii
10.1186/s13071-019-3472-1journal_volume
12pub_type
杂志文章abstract:BACKGROUND:Zoonotic leishmaniosis, caused by the protozoan Leishmania infantum, is a public and animal health problem in Asia, Central and South America, the Middle East and the Mediterranean Basin. Several phlebotomine sand fly species from the subgenus Larroussius are vectors of L. infantum. Data from dogs living in ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3376-0
更新日期:2019-03-26 00:00:00
abstract:BACKGROUND:Red foxes (Vulpes vulpes) are one of the most widespread wild carnivores in the world, being recognized to harbor and transmit a wide range of vector-borne diseases. Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato are zoonotic tick-borne pathogens causing emerging diseases. Wild animals play an...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-1130-9
更新日期:2015-10-08 00:00:00
abstract:BACKGROUND:Two hemoplasma species, Mycoplasma suis and M. parvum, previously known as Eperythrozoon suis and E. parvum, respectively, have been identified in pigs. Swine hemoplasmosis is a global problem, and M. suis infection results in economic losses to pig producers worldwide. This study investigated the frequency ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3638-x
更新日期:2019-07-29 00:00:00
abstract:BACKGROUND:Schistosoma japonicum is a parasitic flatworm that causes human schistosomiasis. Secreted extracellular vesicles (EVs) play a key role in pathogen-host interfaces. Previous studies have shown that S. japonicum adult worms can release microRNA (miRNA)-containing EVs, which can transfer their cargo to mammalia...
journal_title:Parasites & vectors
pub_type: 杂志文章
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更新日期:2016-11-08 00:00:00
abstract:BACKGROUND:Cryptosporidiosis is a gastrointestinal disease affecting many people worldwide. Disease incidence is often unknown and surveillance of human cryptosporidiosis is installed in only a handful of developed countries. A genetic marker that mirrors disease incidence is potentially a powerful tool for monitoring ...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0921-3
更新日期:2015-06-06 00:00:00
abstract:BACKGROUND:Aedes albopictus (Skuse) is an invasive mosquito that has become an important vector of chikungunya, dengue and Zika viruses. In the absence of specific antiviral therapy or a vaccine, vector management is the sole method available for reducing Aedes-induced disease morbidity. Determining the resistance stat...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-017-2581-y
更新日期:2018-01-03 00:00:00
abstract:BACKGROUND:Aedes albopictus is widely distributed across tropical and sub-tropical regions and is associated with the transmission of several arboviruses. Although this species is increasingly relevant to public health due its ability to successfully colonize both urban and rural habitats, favoring the dispersion of vi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-253
更新日期:2014-05-30 00:00:00
abstract:BACKGROUND:The TNF signaling pathway is involved in the regulation of many cellular processes (such as apoptosis and cell proliferation). Previous reports indicated the effect of human TNF-α on metabolism, physiology, gene expression and protein phosphorylation of the human parasite Schistosoma mansoni and suggested th...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04370-1
更新日期:2020-09-25 00:00:00
abstract:BACKGROUND:Thymidylate synthase is a housekeeping gene, designated ancient due to its role in DNA synthesis and ubiquitous phyletic distribution. The genomic sequences were characterized coding for thymidylate synthase in two species of the genus Trichinella, an encapsulating T. spiralis and a non-encapsulating T. pseu...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-7-175
更新日期:2014-04-09 00:00:00
abstract:BACKGROUND:In Brazil, the autochthonous transmission of extra-Amazonian malaria occurs mainly in areas of the southeastern coastal Atlantic Forest, where Anopheles cruzii is the primary vector. In these locations, the population density of the mosquito varies with altitude (5-263 m above sea level), prompting us to hyp...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0581-8
更新日期:2014-12-16 00:00:00
abstract::Presently, 45% of the total human population of Europe, as well as their domestic and companion animals, are exposed to the risk of vector-borne helminths (VBH) causing diseases. A plethora of intrinsic biological and extrinsic factors affect the relationship among helminths, vectors and animal hosts, in a constantly ...
journal_title:Parasites & vectors
pub_type: 杂志文章,评审
doi:10.1186/1756-3305-6-16
更新日期:2013-01-16 00:00:00
abstract:BACKGROUND:The survival of adult female Aedes mosquitoes is a critical component of their ability to transmit pathogens such as dengue viruses. One of the principal determinants of Aedes survival is temperature, which has been associated with seasonal changes in Aedes populations and limits their geographical distribut...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-6-351
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1943-1
更新日期:2017-01-03 00:00:00
abstract:BACKGROUND:Malaria mosquitoes spend a considerable part of their life in the aquatic stage, rendering them vulnerable to interventions directed to aquatic habitats. Recent successes of mosquito larval control have been reported using environmental and biological tools. Here, we report the effects of shading by plants a...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-130
更新日期:2011-07-06 00:00:00
abstract:BACKGROUND:Gastrointestinal nematodes are parasites that commonly infect dogs, and infections can be subclinical or may cause considerable clinical disease. Some species are zoonotic and may also cause clinical disease in humans. Year-round treatment of dogs is recommended to eliminate existing infections, which also i...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-3947-0
更新日期:2020-03-01 00:00:00
abstract:BACKGROUND:Phagocyte-derived extracellular traps (ETs) were recently demonstrated mainly in vertebrate hosts as an important effector mechanism against invading parasites. In the present study we aimed to characterize gastropod-derived invertebrate extracellular phagocyte trap (InEPT) formation in response to larval st...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1961-z
更新日期:2017-01-31 00:00:00
abstract:BACKGROUND:Spotted Fever Group (SFG) Rickettsiae can cause febrile diseases with or without rash in humans worldwide. In Germany only limited data are available about their medical significance. Serological screening tests for antibodies against rickettsiae usually only distinguish between SFG and Typhus Group (TG) Ric...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0745-1
更新日期:2015-03-23 00:00:00
abstract:BACKGROUND:Sand flies are responsible for the transmission of several disease pathogens including Leishmania. Sand flies breed in habitats with high levels of humidity and organic matter. They are nocturnal in nature and peak activity ranges from dusk to dawn. The scientific evidence on breeding ecology and diurnal res...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04154-7
更新日期:2020-06-05 00:00:00
abstract:BACKGROUND:Free-living amoebae of the genus Acanthamoeba are cosmopolitan, widely distributed protozoans that cause a severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). The majority of the increasing number of AK cases are associated with contact lens use. Appropriate eye hygiene and eff...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-020-04453-z
更新日期:2020-12-22 00:00:00
abstract:BACKGROUND:Ecto-Nucleoside Triphosphate Diphosphohydrolases (Ecto-NTPDases) are enzymes that hydrolyze tri- and/or di-phosphate nucleotides. Evidences point to their participation in Trypanosoma cruzi virulence and infectivity. In this work, we evaluate TcNTPDase-1 gene expression in comparison with ecto-NTPDase activi...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-014-0463-0
更新日期:2014-10-05 00:00:00
abstract:BACKGROUND:The cat flea (Ctenocephalides felis) is a blood-feeding ectoparasitic insect and particular nuisance pest of companion animals worldwide. Identification of genes that are differentially expressed in response to feeding is important for understanding flea biology and discovering targets for their control. ME...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0972-5
更新日期:2015-07-14 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-018-2956-8
更新日期:2018-06-25 00:00:00
abstract:BACKGROUND:Canine vector-borne diseases (CVBD) have become a major concern for canine and human public health. The aim of the study described here is to add epidemiological data regarding four pathogens responsible for CVBD, namely anaplasmosis, borreliosis, dirofilariosis and ehrlichiosis in a national survey conducte...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-016-1686-z
更新日期:2016-07-29 00:00:00
abstract:BACKGROUND:With the spread of pyrethroid resistance in mosquitoes, the combination of an insecticide (carbamate or organophosphate) with a repellent (DEET) is considered as a promising alternative strategy for the treatment of mosquito nets and other relevant materials. The efficacy of these mixtures comes from the fac...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-2-17
更新日期:2009-04-16 00:00:00
abstract:BACKGROUND:Both falciparum and vivax malaria were historically prevalent in China with high incidence. With the control efforts, the annual incidence in the whole country has reduced to 0.0001% except in some areas in the southern borders after 2000. Despite this, the re-emergence or outbreak of malaria was unavoidable...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-5-136
更新日期:2012-07-09 00:00:00
abstract:BACKGROUND:The present study was conducted to investigate the serological survey of Toxoplasma antibodies in local.horses from three major regions: a neighbourhood of a city in the North (Sidi Thabet), a neighbourhood of a city on the coast (Monastir) and a neighbourhood of a city in the middle (Battan) of Tunisia (Nor...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/1756-3305-4-218
更新日期:2011-11-22 00:00:00
abstract:BACKGROUND:The Smad proteins function in TGF-β signalling transduction. In the model nematode Caenorhabditis elegans, the co-Smad, DAF-3 mediates R-Smads and performs a central role in DAF-7 signal transduction, regulating dauer formation and reproductive processes. Considering the divergent evolutionary patterns of th...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3855-3
更新日期:2019-12-27 00:00:00
abstract:BACKGROUND:Epidemics of dengue, chikungunya and Zika are a growing threat to areas where Aedes aegypti are present. The efficacy of chemical control of Ae. aegypti is threatened by the increasing frequency of insecticide resistance. The objective of this study was to determine the susceptibility status as well as the b...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-019-3739-6
更新日期:2019-10-22 00:00:00
abstract:BACKGROUND:As part of efforts to more fully understand the potential risks posed by West Nile virus (WNV) and Usutu virus (USUV) in the UK, and following on from previous reports of a potential bridge vector Culex modestus for these viruses, at wetland sites in North Kent, mosquito surveillance was undertaken more wide...
journal_title:Parasites & vectors
pub_type: 杂志文章
doi:10.1186/s13071-015-0705-9
更新日期:2015-02-10 00:00:00
abstract:BACKGROUND:Mosquito-borne arboviruses, like dengue virus, continue to cause significant global morbidity and mortality, particularly in Southeast Asia. When the infectious mosquitoes probe into human skin for a blood meal, they deposit saliva containing a myriad of pharmacologically active compounds, some of which alte...
journal_title:Parasites & vectors
pub_type: 临床试验,杂志文章
doi:10.1186/s13071-018-3224-7
更新日期:2018-12-20 00:00:00