Abstract:
Background:Metabolic syndrome is characterized by macrophage infiltration and inflammatory responses-metaflammation in adipose tissue. IL-6 and IL-1β could mediate the inflammatory responses in macrophage stimulated-preadipocytes by modulating MAPK and NF-κB pathways. To test this hypothesis we used antibodies to block IL-6 and IL-1β action in macrophage conditioned medium (MacCM)-stimulated human white preadipocytes. Moreover, as interventions that prevent this could potentially be used to treat or prevent metabolic syndrome, and 1α,25(OH)2D3 has previously been reported to exert an anti-inflammatory action on macrophage-stimulated adipocytes, in this study we also investigated whether 1α,25(OH)2D3 could attenuate inflammatory responses in MacCM-stimulated preadipocytes, and explored the potential anti-inflammatory mechanisms. Methods:Human white preadipocytes were cultured with 25% MacCM for 24 h to elicit inflammatory responses. This was confirmed by measuring the concentrations and mRNA levels of major pro-inflammatory factors [IL-1β, IL-6, IL-8, monocyte chemoattractant protein (MCP)-1 and regulated on activation, normal T cell expressed and secreted (RANTES)] by ELISA and qPCR, respectively. IL-6 and IL-1β actions were blocked using IL-6 antibody (300 ng/ml) and IL-1β antibody (15 μg/ml), respectively. Potential anti-inflammatory effects of 1α,25(OH)2D3 were investigated by pre-treatment and treatment of 1α,25(OH)2D3 (0.01 to 10 nM) for 48 h in MacCM-stimulated preadipocytes. In parallel, western blotting was used to determine inflammatory signaling molecules including relA of the NF-κB pathway and p44/42 MAPK modified during these processes. Results:MacCM enhanced the secretion and gene expression of IL-1β, IL-6, IL-8, MCP-1 and RANTES by increasing the phosphorylation levels of relA and p44/42 MAPK in preadipocytes, whereas blocking IL-6 and IL-1β action inhibited the inflammatory responses by decreasing p44/42 MAPK and relA phosphorylation, respectively. Furthermore, 10 nM of 1α,25(OH)2D3 generally inhibited the IL-6 and IL-1β-mediated inflammatory responses, and reduced both p44/42 MAPK and relA phosphorylation in MacCM-stimulated preadipocytes. Conclusions:1α,25(OH)2D3 attenuates IL-6 and IL-1β-mediated inflammatory responses, probably by inhibiting p44/42 MAPK and relA phosphorylation in MacCM-stimulated human white preadipocytes.
journal_name
Diabetol Metab Syndrjournal_title
Diabetology & metabolic syndromeauthors
Zhu J,Bing C,Wilding JPHdoi
10.1186/s13098-019-0405-2subject
Has Abstractpub_date
2019-01-25 00:00:00pages
9issn
1758-5996pii
405journal_volume
11pub_type
杂志文章abstract:Background:Exercise can disrupt homeostasis and trigger many adaptive responses in different hormonal axes. The study of hormonal interactions with physical activity is highly complex due to the number of variables, such as exercise duration, exercise intensity, individual level of training, circadian rhythm, nutrition...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-019-0414-1
更新日期:2019-02-15 00:00:00
abstract:BACKGROUND:Reduced insulin sensitivity not only contributes to the pathogenesis of type 2 diabetes but is also linked to multiple metabolic risk factors and cardiovascular diseases (CVD). A prolonged heart rate-corrected QT interval (QTc interval) is related to ventricular arrhythmias and CVD mortality and exhibits a h...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-017-0268-3
更新日期:2017-09-11 00:00:00
abstract:BACKGROUND:Polymorphisms in genes encoding adiponectin (ADIPOQ) and interleukin-6 (IL6) have been associated with adiposity and obese-related phenotypes. This study investigated the relationship of ADIPOQ and IL6 gene polymorphisms with pro-inflammatory and cardiometabolic markers in obese patients. METHODS:Anthropome...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-015-0027-2
更新日期:2015-04-11 00:00:00
abstract:BACKGROUND:Hypertriglyceridemia (HTG) is considered an independent risk factor for major adverse cardiovascular events (MACE). METHODS:This study analyzed the effects of various agents on MACE risk reduction in HTG (serum triglyceride ≥ 150 mg/dl) populations by performing a network meta-analysis. We performed a frequ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-021-00626-7
更新日期:2021-01-29 00:00:00
abstract:UNLABELLED: BACKGROUND:Achieving good glycemic control is of paramount importance in the reduction of diabetes mellitus (DM) associated morbidity and mortality. Insulin plays a key role in the management of DM but unfortunately whilst some healthcare providers present insulin as a treatment of last resort , patients o...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-4-50
更新日期:2012-12-02 00:00:00
abstract:Background:To investigate the association of low carbohydrate diet (LCD) score with the risk of type 2 diabetes among adults. Methods:This cohort study was conducted on 4356 healthy participants aged ≥ 19 years old, who were followed-up for a mean duration of 3 years within the framework of the Tehran Lipid and Glucos...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-020-00596-2
更新日期:2020-10-07 00:00:00
abstract:Background:Endothelial dysfunction (ED) is a hallmark in type 2 diabetes mellitus (T2DM) that favor both atherogenesis and ischemia and reperfusion injury (IRI). Sodium-glucose-2 co-transporter inhibitors (SGLT2i) may hypothetically improve microvascular and macrovascular functions via a broad spectrum of mechanisms, b...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-019-0457-3
更新日期:2019-07-31 00:00:00
abstract:BACKGROUND:Adipokines, e.g. TNFα, IL-6 and leptin increase insulin resistance, and consequent hyperinsulinaemia influences breast cancer progression. Beside its mitogenic effects, insulin may influence adipokine production from adipocyte stromal cells and paracrine enhancement of breast cancer cell growth. In contrast,...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-3-16
更新日期:2011-07-20 00:00:00
abstract:Background:The role of serum fibroblast growth factor 19 (FGF19) in arteriosclerosis is not well known. In the present study, we aimed to explore whether serum FGF19 levels were related to arteriosclerosis parameters, including arterial stiffness and atherogenic index of plasma (AIP), in patients with type 2 diabetes (...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-020-00552-0
更新日期:2020-05-20 00:00:00
abstract:Purpose:People with obesity have a compromised browning capacity of adipose tissue when faced with sympathetic stimuli. This study aimed to determine whether norepinephrine treatment can enhance the induction of precursor cells from human white adipose tissue to differentiate into adipocytes that express key markers of...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-020-00599-z
更新日期:2020-10-22 00:00:00
abstract:BACKGROUND:Vildagliptin, a DPP-4 inhibitor widely used for the treatment of type 2 diabetes mellitus (T2DM), shows beneficial effects on endothelial function. This study aims to evaluate the effect of vildagliptin on endothelial function and arterial stiffness in patients with T2DM and hypertension. METHODS:Fifty over...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-015-0062-z
更新日期:2015-08-26 00:00:00
abstract:Background:In type I diabetes mellitus (T1DM) pancreatic β cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet transplant could be an alternative in patients with poor glycemic control, but inefficient islet purificati...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-020-00573-9
更新日期:2020-08-05 00:00:00
abstract:BACKGROUND:Many patients with chronic heart failure (CHF) are believed to have unrecognized diabetes, which is associated with a worse prognosis. This study aimed to describe glucose tolerance in a general stable CHF population and to identify determinants of glucose tolerance focusing on body composition and skeletal ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-6-44
更新日期:2014-03-27 00:00:00
abstract:BACKGROUND:Many self-management programs have been developed so far. Their effectiveness varies. The program 'Beyond Good Intentions' (BGI) is based on proactive coping and has proven to be (cost-) effective in achieving reductions in BMI and blood pressure in screen-detected type 2 diabetes patients up until nine mont...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-016-0199-4
更新日期:2016-12-20 00:00:00
abstract:Background:Several studies suggest that statin may increase the risk of new-onset diabetes mellitus (NODM). This study aimed to evaluate the association between the duration and recent use of statin, and the risk of NODM, based on population-based data sets. Methods:We used the South Korean National Health Insurance S...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-019-0427-9
更新日期:2019-04-22 00:00:00
abstract:BACKGROUND:Distal symmetric polyneuropathy (DSPN) is the most common complication of type 2 diabetes mellitus (T2DM) and the most common form of peripheral neuropathy. DSPN increases the risk of foot ulceration up to seven-fold, and is a significant risk factor in more than 60 % of the amputations of the lower limbs in...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-016-0146-4
更新日期:2016-04-11 00:00:00
abstract:BACKGROUND:In most individuals, obesity and insulin resistance coexist. However, some individuals have excessive adipose tissue mass but remain insulin sensitive. Moreover, lean individuals can develop acute inflammation-induced insulin resistance, even without excess adipose tissue mass. OBJECTIVE:Our aim was to comp...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-016-0125-9
更新日期:2016-02-09 00:00:00
abstract:BACKGROUND:Type 1 diabetes mellitus in pregnant women is associated with an increased risk of congenital malformations, obstetric complications, neonatal morbidity, and mortality. Our aim was to evaluate which factors from the first trimester of pregnancy have a significant impact on the pregnancy outcomes of women wit...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-6-125
更新日期:2014-11-22 00:00:00
abstract:BACKGROUND:The single-center, open-label, four-arm, exploratory study investigates the relation of different anti-diabetics to serum levels of active TGF-β, a known pro-fibrotic stimulus, before and after a defined test meal. FINDINGS:We investigated sera of patients with type 2 diabetes mellitus (T2DM) treated with m...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-5-48
更新日期:2013-08-31 00:00:00
abstract:BACKGROUND:Liraglutide, a glucagon-like peptide-1 (GLP-1) analogue, has been shown to possess pleiotropic effects including body weight reduction. However, long-term effect of liraglutide on body weight and glycemic control has not been elucidated in Japanese type 2 diabetes (T2D) subjects. Present study investigates w...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-6-95
更新日期:2014-09-08 00:00:00
abstract:Background:The present study identified whether glycemic variability (GV) was associated with vascular calcification and explored the underlying mechanisms. Methods:Eighty-four consecutive type 2 diabetic patients with unstable angina (UA) were included from January 2018 to June 2018 to calculate calcification scores ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-019-0464-4
更新日期:2019-08-19 00:00:00
abstract:Background:Vitamins are essential micronutrients with antioxidant potential that may provide a complementary treatment for patients with chronic diseases. Our aim was to assess the effect of vitamin supplementation on the antioxidant status and glycemic index of type 2 diabetes mellitus patients. Methods:We performed ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-018-0318-5
更新日期:2018-03-14 00:00:00
abstract:BACKGROUND:The oral DPP-4 inhibitors are new incretin-based therapies for treatment of type 2 diabetes. To assess the efficacy and safety of three DPP-4 inhibitors (Saxagliptin, Sitagliptin and Vildagliptin) as add-on therapy to dual combination of traditional oral hypoglycemic agents in Chinese type 2 diabetes patient...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-6-69
更新日期:2014-05-31 00:00:00
abstract:Background:Obesity is associated with several comorbidities, such as cardiovascular disease and type 2 diabetes mellitus, and may have its origin in early life stages, such as in the lactation period, through metabolic programming. Physical activity aids in decreasing the chances of developing cardiovascular and metabo...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-018-0374-x
更新日期:2018-09-26 00:00:00
abstract:OBJECTIVE:The primary purpose of this study was to examine the construct validity of a continuous metabolic syndrome score (cMetS) in children. The secondary purpose was to identify a cutpoint value(s) for an adverse cMetS based on receiver operating characteristic (ROC) curve analysis. METHODS:378 children aged 7 to ...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-2-8
更新日期:2010-01-28 00:00:00
abstract:Background:Whether polymorphisms in tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10) or adiponectin (ADIPOQ) influence the risk of gestational diabetes mellitus (GDM) or not remain inconclusive. Therefore, the authors conducted a meta-analysis to robustly assess relationships between polymo...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-020-00582-8
更新日期:2020-09-17 00:00:00
abstract:Background:The prevalence of diabetes and prediabetes were estimated to be 10.9% and 35.7% in the Chinese adult population, respectively, and the middle-aged and elderly Chinese are at even higher risk of diabetes and prediabetes than younger population. With the increasing trend of aging in China, the burden of diabet...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-019-0418-x
更新日期:2019-03-05 00:00:00
abstract:BACKGROUND:Using patient data from the GetGoal-Duo1, -L, and L-Asia trials, the objectives of this study were to evaluate and compare the impact of lixisenatide once-daily add-on treatment to basal insulin therapy ±oral antidiabetic drugs (OADs) among type 2 diabetes (T2DM) patients subdivided into groups, based on the...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-015-0104-6
更新日期:2015-11-21 00:00:00
abstract:Background:Cardiovascular disease (CVD) is the leading cause of morbidity and mortality in patients with type 2 diabetes (T2DM). Carotid intima-media thickness (CIMT) is considered a preclinical stage of atherosclerosis. Therefore, it is necessary to identify the related risk factors for CIMT to facilitate the early pr...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/s13098-020-0517-8
更新日期:2020-01-28 00:00:00
abstract:BACKGROUND:Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of nitric oxide (NO) synthetase. Elevated ADMA reduces NO formation and is associated with endothelial dysfunction. The aims of this study were to evaluate endothelial function and the cardiovascular risk (CVR) profile in patients with...
journal_title:Diabetology & metabolic syndrome
pub_type: 杂志文章
doi:10.1186/1758-5996-6-109
更新日期:2014-10-14 00:00:00