Activating PTEN Tumor Suppressor Expression with the CRISPR/dCas9 System.

abstract：:Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability of other efficiently expressed luciferases using different substra...

journal_title：Molecular therapy. Nucleic acids

authors： Maguire CA,Bovenberg MS,Crommentuijn MH,Niers JM,Kerami M,Teng J,Sena-Esteves M,Badr CE,Tannous BA

更新日期：2013-06-18 00:00:00

abstract：:Vascular endothelial growth factors (VEGFs) are hypoxia-inducible secreted proteins to promote angiogenesis, in which VEGF-A is an important molecule that binds and activates VEGF receptor-1 (VEGFR-1) and VEGFR-2. In this study, two DNA aptamers, Apt01 and Apt02, were successfully isolated by alternating consecutive s...

journal_title：Molecular therapy. Nucleic acids

authors： Yoshitomi T,Hayashi M,Oguro T,Kimura K,Wayama F,Furusho H,Yoshimoto K

更新日期：2020-03-06 00:00:00

abstract：:Selenium deficiency has been proven to induce calcium disorders in the chicken heart. However, detailed regulatory mechanisms, e.g., the long noncoding RNA (lncRNA)-microRNA (miRNA)-mRNA regulatory axis, have not yet been described. Here, we point out lnc-2315, miR-1594, and Troponin T (TNNT2) based on the results of ...

journal_title：Molecular therapy. Nucleic acids

authors： Yang J,Gong Y,Cai J,Liu Q,Zhang Z

更新日期：2019-12-06 00:00:00

abstract：:Adeno-associated virus (AAV) is the most common vector for clinical gene therapy of the CNS. This popularity originates from a high safety record and the longevity of transgene expression in neurons. Nevertheless, clinical efficacy for CNS indications is lacking, and one reason for this is the relatively limited sprea...

journal_title：Molecular therapy. Nucleic acids

authors： Kanaan NM,Sellnow RC,Boye SL,Coberly B,Bennett A,Agbandje-McKenna M,Sortwell CE,Hauswirth WW,Boye SE,Manfredsson FP

更新日期：2017-09-15 00:00:00

abstract：:Glioblastoma is the most common malignant primary brain tumor among adults and one of the most lethal cancers. It is characterized by the deregulation of signaling pathways involving proliferation, growth, survival, and other factors. MicroRNAs (miRNAs) play a role in the regulation of genes by affecting the 3' untran...

journal_title：Molecular therapy. Nucleic acids

authors： Moradimotlagh A,Arefian E,Rezazadeh Valojerdi R,Ghaemi S,Jamshidi Adegani F,Soleimani M

更新日期：2020-03-06 00:00:00

abstract：:Cutaneous squamous cell carcinoma (CSCC) is a malignant proliferation of cutaneous epithelium that has been observed to have an alarming rise in incidence. Numerous studies have demonstrated microRNAs (miRNAs or miRs) as important biomarkers in the diagnosis, prognosis, and treatment of CSCC. This study aims to invest...

journal_title：Molecular therapy. Nucleic acids

authors： Ting W,Feng C,Zhang M,Long F,Bai M

更新日期：2020-09-04 00:00:00

abstract：:Next-generation sequencing technology has been commonly applied to detect mitochondrial DNA (mtDNA) mutations, which are reported to be strongly associated with cancers. However, several key challenges still exist regarding bioinformatics analysis of mtDNA sequencing data that greatly affect the detection accuracy of ...

journal_title：Molecular therapy. Nucleic acids

authors： Guo S,Zhou K,Yuan Q,Su L,Liu Y,Ji X,Gu X,Guo X,Xing J

更新日期：2020-11-11 00:00:00

abstract：:We took advantage of the ability of human telomeres to silence neighboring genes (telomere position effect or TPE) to design a high-throughput screening assay for drugs altering telomeres. We identified, for the first time, that two dietary flavones, acacetin and chrysin, are able to specifically alleviate TPE in huma...

journal_title：Molecular therapy. Nucleic acids

authors： Boussouar A,Barette C,Nadon R,Saint-Léger A,Broucqsault N,Ottaviani A,Firozhoussen A,Lu Y,Lafanechère L,Gilson E,Magdinier F,Ye J

更新日期：2013-08-20 00:00:00

abstract：:E-selectin is an adhesion molecule expressed on the luminal surface of inflamed blood vessels that mediates hematogenous metastasis by assisting shear-resistant adhesion of circulating tumor cells to the vessel surface under dynamic blood flow. Previously, we developed an E-selectin antagonistic thioaptamer (ESTA) for...

journal_title：Molecular therapy. Nucleic acids

authors： Morita Y,Kamal M,Kang SA,Zhang R,Lokesh GL,Thiviyanathan V,Hasan N,Woo S,Zhao D,Leslie M,Suh S,Razaq W,Rui H,Gorenstein DG,Volk DE,Tanaka T

更新日期：2016-12-13 00:00:00

abstract：:Numerous studies have suggested that dysregulated long noncoding RNAs (lncRNAs) contributed to the development and progression of many cancers. lncRNA OIP5 antisense RNA 1 (OIP5-AS1) has been reported to be increased in several cancers. However, the roles of OIP5-AS1 in liver hepatocellular carcinoma (LIHC) remain to ...

journal_title：Molecular therapy. Nucleic acids

authors： Ma YS,Chu KJ,Ling CC,Wu TM,Zhu XC,Liu JB,Yu F,Li ZZ,Wang JH,Gao QX,Yi B,Wang HM,Gu LP,Li L,Tian LL,Shi Y,Jiang XQ,Fu D,Zhang XW

更新日期：2020-09-04 00:00:00

abstract：:Reactivation of γ-globin expression has been shown to ameliorate disease phenotypes associated with mutations in the adult β-globin gene, including sickle cell disease. Specific mutations in the promoter of the γ-globin genes are known to prevent repression of the genes in the adult and thus lead to hereditary persist...

journal_title：Molecular therapy. Nucleic acids

authors： Hossain MA,Shen Y,Knudson I,Thakur S,Stees JR,Qiu Y,Pace BS,Peterson KR,Bungert J

更新日期：2016-10-18 00:00:00

abstract：:[This corrects the article DOI: 10.1038/mtna.2016.46.]. ...

journal_title：Molecular therapy. Nucleic acids

authors： Cao L,Han G,Lin C,Gu B,Gao X,Moulton HM,Seow Y,Yin H

更新日期：2020-11-19 00:00:00

abstract：:MicroRNA (miR)-137 is highly expressed in the brain and plays a crucial role in the development and prognosis of glioma. In this review, we aim to summarize the latest findings regarding miR-137 in glioma cell apoptosis, proliferation, migration, invasion, angiogenesis, drug resistance, and cancer treatment. In additi...

journal_title：Molecular therapy. Nucleic acids

authors： Wang Y,Chen R,Zhou X,Guo R,Yin J,Li Y,Ma G

更新日期：2020-09-04 00:00:00

abstract：:Gastric cancer (GC) is one of the leading causes of cancer-related deaths worldwide. Accumulating evidence reveals the significance of long non-coding RNAs (lncRNAs) in various cancers. The current study aimed to evaluate the role of GATA6 antisense RNA 1 (GATA6-AS1) in the epithelial-mesenchymal transition (EMT) and ...

journal_title：Molecular therapy. Nucleic acids

authors： Li ZT,Zhang X,Wang DW,Xu J,Kou KJ,Wang ZW,Yong G,Liang DS,Sun XY

更新日期：2020-03-06 00:00:00

abstract：:With the development of science and biotechnology, many evidences show that ncRNAs play an important role in the development of important biological processes, especially in chromatin modification, cell differentiation and proliferation, RNA progressing, human diseases, etc. Moreover, lncRNAs account for the majority ...

journal_title：Molecular therapy. Nucleic acids

authors： Zhao Q,Yu H,Ming Z,Hu H,Ren G,Liu H

更新日期：2018-12-07 00:00:00

abstract：:Keratinocyte proliferation and migration are crucial steps during skin wound healing. The functional role of microRNAs (miRs) remains relatively unknown during this process. miR-93 levels have been reported to increase within 24 h of skin wound healing; however, whether miR-93-3p or miR-93-5p plays a specific role in ...

journal_title：Molecular therapy. Nucleic acids

authors： Feng X,Zhou S,Cai W,Guo J

更新日期：2020-11-26 00:00:00

abstract：:Diabetic retinopathy (DR) is a serious complication of diabetes, which is the main cause of blindness among adults. Traditional Chinese medicines (TCMs) have been proven to delay the development of DR. Nonetheless, the effect of Schizandrin A (SchA) on DR remains uninvestigated. The present study aimed to probe the pr...

journal_title：Molecular therapy. Nucleic acids

authors： Dong Y,Qian C,Wan G,Yan P,Liang S,Wang J

更新日期：2020-03-06 00:00:00

abstract：:Circular RNA (circRNA) is a novel subclass of noncoding-RNA molecules that participate in development and progression of a variety of human diseases via sponging microRNAs (miRNAs), but the role of circRNAs in Hashimoto's thyroiditis (HT) has not been defined. In this study, peripheral blood samples from five patients...

journal_title：Molecular therapy. Nucleic acids

authors： Xiong S,Peng H,Ding X,Wang X,Wang L,Wu C,Wang S,Xu H,Liu Y

更新日期：2019-09-06 00:00:00

abstract：:Acid fibroblast growth factor (aFGF) has shown neuroprotection in Alzheimer's disease (AD) models in previous studies, yet its mechanism is still uncertain. Here we report that the efficacy of Tat-haFGF14-154 is markedly increased when loaded cationic liposomes for intranasal delivery are intranasally administered to ...

journal_title：Molecular therapy. Nucleic acids

authors： Meng T,Cao Q,Lei P,Bush AI,Xiang Q,Su Z,He X,Rogers JT,Chiu IM,Zhang Q,Huang Y

更新日期：2017-06-16 00:00:00

abstract：:Due to highly enzymatic d-stereoselectivity, l-nucleotides (l-2'-deoxynucleoside 5'-triphosphates [l-dNTPs]) are not natural targets of polymerases. In this study, we synthesized series of l-thymidine (l-T)-modified DNA strands and evaluated the processivity of nucleotide incorporation for transcription by T7 RNA poly...

journal_title：Molecular therapy. Nucleic acids

authors： Liu Q,Ke Y,Kan Y,Tang X,Li X,He Y,Wu L

更新日期：2020-09-04 00:00:00

abstract：:Recent studies have shown the effect of microRNAs on HSC activation and transformation, which is essential for the pathogenesis of liver fibrosis. In our study, we explored the role of miR-185 in liver fibrosis. Plasma miR-185 was detected in hepatitis B virus-related liver fibrosis patients (S2/3, n = 10) by Illumina...

journal_title：Molecular therapy. Nucleic acids

authors： Zhou L,Liu S,Han M,Ma Y,Feng S,Zhao J,Lu H,Yuan X,Cheng J

更新日期：2018-03-02 00:00:00

abstract：:Antiviral strategies targeting hijacked cellular processes are less easily evaded by the virus than viral targets. If selective for viral functions, they can have a high therapeutic index. We used RNA trans-splicing to deliver the herpes simplex virus thymidine kinase-ganciclovir (HSV-tk/GCV) cell suicide system into ...

journal_title：Molecular therapy. Nucleic acids

authors： Ingemarsdotter CK,Poddar S,Mercier S,Patzel V,Lever AML

更新日期：2017-06-16 00:00:00

abstract：:Non-coding RNAs play an important role in the pathogenesis of pulmonary arterial hypertension (PAH). The aim of this study was to characterize the therapeutic role of melatonin as well as the underlying molecular mechanism (its effects on the expression of H19 and its downstream signaling pathways) in the treatment of...

journal_title：Molecular therapy. Nucleic acids

authors： Wang R,Zhou S,Wu P,Li M,Ding X,Sun L,Xu X,Zhou X,Zhou L,Cao C,Fei G

更新日期：2018-12-07 00:00:00

abstract：:Human spermatogonial stem cells (SSCs) could have significant applications in reproductive medicine and regenerative medicine because of their great plasticity. The fate determinations of human SSCs are mediated by epigenetic factors. However, nothing is known about the regulation of non-coding RNA on human SSCs. Here...

journal_title：Molecular therapy. Nucleic acids

authors： Zhou F,Yuan Q,Zhang W,Niu M,Fu H,Qiu Q,Mao G,Wang H,Wen L,Wang H,Lu M,Li Z,He Z

更新日期：2018-09-07 00:00:00

abstract：:The role of microglia in the pathophysiology of ischemic retinal diseases has been studied extensively. Exosomes from microglial cells exert protective effects during several nervous system diseases, but their roles in hypoxia-induced retinopathy remain unclear. In our study, exosomes derived from microglial cells wer...

journal_title：Molecular therapy. Nucleic acids

authors： Xu W,Wu Y,Hu Z,Sun L,Dou G,Zhang Z,Wang H,Guo C,Wang Y

更新日期：2019-06-07 00:00:00

abstract：:MicroRNAs (miRNAs) are an important class of small noncoding RNA molecules that serve as excellent biomarkers of various diseases. However, current miRNA biomarkers, including those comprised of multiple miRNAs, work at a single-miRNA level but not at a miRNA-set level, which is defined as a group of miRNAs sharing co...

journal_title：Molecular therapy. Nucleic acids

authors： Shi J,Cui Q

更新日期：2020-09-04 00:00:00

abstract：:Bone marrow-derived mesenchymal stem cells (BMSCs) have been suggested to possess the capacity to differentiate into different cell lineages. Maintaining a balanced stem cell differentiation program is crucial to the bone microenvironment and bone development. MicroRNAs (miRNAs) have played a critical role in regulati...

journal_title：Molecular therapy. Nucleic acids

authors： Li Y,Yang F,Gao M,Gong R,Jin M,Liu T,Sun Y,Fu Y,Huang Q,Zhang W,Liu S,Yu M,Yan G,Feng C,He M,Zhang L,Ding F,Ma W,Bi Z,Xu C,Yuan Y,Cai B,Yang L

更新日期：2019-09-06 00:00:00

abstract：:Circular RNA (circRNA), as a kind of novel identified non-coding RNA, has become the focus of attention for its vital physiological and pathological roles. However, the function and mechanism of circRNAs in the regulation of cancer progression are largely unknown. In the present study we found a circRNA termed circHMC...

journal_title：Molecular therapy. Nucleic acids

authors： Song X,Liang Y,Sang Y,Li Y,Zhang H,Chen B,Du L,Liu Y,Wang L,Zhao W,Ma T,Wang C,Yang Q

更新日期：2020-06-05 00:00:00

abstract：:Monogenic disorders (MGDs), which are caused by single gene mutations, have a serious effect on human health. Among these, β-thalassemia (β-thal) represents one of the most common hereditary hematological diseases caused by mutations in the human hemoglobin β (HBB) gene. The technologies of induced pluripotent stem ce...

journal_title：Molecular therapy. Nucleic acids

authors： Liu Y,Yang Y,Kang X,Lin B,Yu Q,Song B,Gao G,Chen Y,Sun X,Li X,Bu L,Fan Y

更新日期：2017-03-17 00:00:00

abstract：:Blocking the immunoinhibitory PD-1:PD-L1 pathway using monoclonal antibodies has led to dramatic clinical responses by reversing tumor immune evasion and provoking robust and durable antitumor responses. Anti-PD-1 antibodies have now been approved for the treatment of melanoma, and are being clinically tested in a num...

journal_title：Molecular therapy. Nucleic acids

authors： Prodeus A,Abdul-Wahid A,Fischer NW,Huang EH,Cydzik M,Gariépy J

更新日期：2015-04-28 00:00:00