当前位置： SCI文献检索 > Molecular Therapy-Nucleic Acids期刊下所有文献 > Binding and Structural Properties of DNA Aptamers with VEGF-A-Mimic Activity.

Binding and Structural Properties of DNA Aptamers with VEGF-A-Mimic Activity.

Abstract：

:Vascular endothelial growth factors (VEGFs) are hypoxia-inducible secreted proteins to promote angiogenesis, in which VEGF-A is an important molecule that binds and activates VEGF receptor-1 (VEGFR-1) and VEGFR-2. In this study, two DNA aptamers, Apt01 and Apt02, were successfully isolated by alternating consecutive systematic evolution of ligands by exponential enrichment (SELEX) against VEGFR-1 and -2 using deep sequencing analysis in an early selection round. Their binding affinities for VEGFR-2 were lower than that of VEGFR-1, which is similar to that of VEGF-A. Structural analyses with the measurements of circular dichroism spectra and ultraviolet melting curve showed that Apt01 possessed the stem-loop structure in the molecule, whereas Apt02 formed G-quadruplex structures. In addition, Apt02 accelerated a tube formation of human umbilical vein endothelial cells faster than Apt01, which was affected by difference of binding affinity and nuclease resistance due to G-quadruplex structures. These results demonstrated that Apt02 might have a potential to function as an alternative to VEGF-A.

journal_name

Mol Ther Nucleic Acids

journal_title

Molecular therapy. Nucleic acids

authors

Yoshitomi T,Hayashi M,Oguro T,Kimura K,Wayama F,Furusho H,Yoshimoto K

doi

10.1016/j.omtn.2019.12.034

subject

Has Abstract

pub_date

2020-03-06 00:00:00

pages

1145-1152

issn

2162-2531

pii

S2162-2531(20)30018-4

journal_volume

19

pub_type

杂志文章

相关文献

Molecular Therapy-Nucleic Acids文献大全
  • Inhibition of Murine Cytomegalovirus Infection in Animals by RNase P-Associated External Guide Sequences.

    abstract：:External guide sequence (EGS) RNAs are associated with ribonuclease P (RNase P), a tRNA processing enzyme, and represent promising agents for gene-targeting applications as they can direct RNase-P-mediated cleavage of a target mRNA. Using murine cytomegalovirus (MCMV) as a model system, we examined the antiviral effec...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2017.10.007

    authors： Li W,Sheng J,Xu M,Vu GP,Yang Z,Liu Y,Sun X,Trang P,Lu S,Liu F

    更新日期：2017-12-15 00:00:00

  • Progress in RNAi-mediated Molecular Therapy of Acute and Chronic Myeloid Leukemia.

    abstract：:Leukemias arise from genetic alterations in normal hematopoietic stem or progenitor cells, leading to impaired regulation of proliferation, differentiation, apoptosis, and survival of the transformed cells. With the advent of RNA interference (RNAi) and the short interfering RNA (siRNA) as its pharmacological mediator...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章,评审

    doi：10.1038/mtna.2015.13

    authors： Landry B,Valencia-Serna J,Gul-Uludag H,Jiang X,Janowska-Wieczorek A,Brandwein J,Uludag H

    更新日期：2015-05-12 00:00:00

  • Identification and Characterization of an eIF4e DNA Aptamer That Inhibits Proliferation With High Throughput Sequencing.

    abstract：:Development of DNA aptamer screens that are both simple and informative can increase the success rate of DNA aptamer selection and induce greater adoption. High eIF4e levels contribute to malignancies, thus eIF4e presents itself as a valuable target for DNA aptamer-based inhibition screen. Here, we demonstrate a metho...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2014.70

    authors： Guo WM,Kong KW,Brown CJ,Quah ST,Yeo HL,Hoon S,Seow Y

    更新日期：2014-12-16 00:00:00

  • The Processing, Gene Regulation, Biological Functions, and Clinical Relevance of N4-Acetylcytidine on RNA: A Systematic Review.

    abstract：:N4-acetylcytidine (ac4C) is often considered to be a conservative, chemically modified nucleoside present on tRNA and rRNA. Recent studies have shown extensive ac4C modifications in human and yeast mRNAs. ac4C helps to correctly read codons during translation and improves translation efficiency and the stability of mR...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章,评审

    doi：10.1016/j.omtn.2020.01.037

    authors： Jin G,Xu M,Zou M,Duan S

    更新日期：2020-06-05 00:00:00

  • Targeting the IGF1R Pathway in Breast Cancer Using Antisense lncRNA-Mediated Promoter cis Competition.

    abstract：:Aberrant insulin-like growth factor I receptor (IGF1R) signaling pathway serves as a well-established target for cancer drug therapy. The intragenic antisense long noncoding RNA (lncRNA) IRAIN, a putative tumor suppressor, is downregulated in breast cancer cells, while IGF1R is overexpressed, leading to an abnormal IG...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.04.013

    authors： Pian L,Wen X,Kang L,Li Z,Nie Y,Du Z,Yu D,Zhou L,Jia L,Chen N,Li D,Zhang S,Li W,Hoffman AR,Sun J,Cui J,Hu JF

    更新日期：2018-09-07 00:00:00

  • miR-185 Inhibits Fibrogenic Activation of Hepatic Stellate Cells and Prevents Liver Fibrosis.

    abstract：:Recent studies have shown the effect of microRNAs on HSC activation and transformation, which is essential for the pathogenesis of liver fibrosis. In our study, we explored the role of miR-185 in liver fibrosis. Plasma miR-185 was detected in hepatitis B virus-related liver fibrosis patients (S2/3, n = 10) by Illumina...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2017.11.010

    authors： Zhou L,Liu S,Han M,Ma Y,Feng S,Zhao J,Lu H,Yuan X,Cheng J

    更新日期：2018-03-02 00:00:00

  • Repair of Mybpc3 mRNA by 5'-trans-splicing in a Mouse Model of Hypertrophic Cardiomyopathy.

    abstract：:RNA trans-splicing has been explored as a therapeutic option for a variety of genetic diseases, but not for cardiac genetic disease. Hypertrophic cardiomyopathy (HCM) is an autosomal-dominant disease, characterized by left ventricular hypertrophy (LVH) and diastolic dysfunction. MYBPC3, encoding cardiac myosin-binding...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2013.31

    authors： Mearini G,Stimpel D,Krämer E,Geertz B,Braren I,Gedicke-Hornung C,Précigout G,Müller OJ,Katus HA,Eschenhagen T,Voit T,Garcia L,Lorain S,Carrier L

    更新日期：2013-07-02 00:00:00

  • Expanding the Repertoire of Target Sites for Zinc Finger Nuclease-mediated Genome Modification.

    abstract：:Recent studies have shown that zinc finger nucleases (ZFNs) are powerful reagents for making site-specific genomic modifications. The generic structure of these enzymes includes a ZF DNA-binding domain and nuclease domain (Fn) are separated by an amino acid "linker" and cut genomic DNA at sites that have a generic str...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2013.13

    authors： Wilson KA,McEwen AE,Pruett-Miller SM,Zhang J,Kildebeck EJ,Porteus MH

    更新日期：2013-04-30 00:00:00

  • Overexpressed lncRNA GATA6-AS1 Inhibits LNM and EMT via FZD4 through the Wnt/β-Catenin Signaling Pathway in GC.

    abstract：:Gastric cancer (GC) is one of the leading causes of cancer-related deaths worldwide. Accumulating evidence reveals the significance of long non-coding RNAs (lncRNAs) in various cancers. The current study aimed to evaluate the role of GATA6 antisense RNA 1 (GATA6-AS1) in the epithelial-mesenchymal transition (EMT) and ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.09.034

    authors： Li ZT,Zhang X,Wang DW,Xu J,Kou KJ,Wang ZW,Yong G,Liang DS,Sun XY

    更新日期：2020-03-06 00:00:00

  • CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells.

    abstract：:Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notabl...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章,评审

    doi：10.1038/mtna.2016.95

    authors： Wang X,Huang X,Fang X,Zhang Y,Wang W

    更新日期：2016-11-15 00:00:00

  • Endogenous MicroRNA Competition as a Mechanism of shRNA-Induced Cardiotoxicity.

    abstract：:Gene knockdown using short hairpin RNAs (shRNAs) is a promising strategy for targeting dominant mutations; however, delivering too much shRNA can disrupt the processing of endogenous microRNAs (miRNAs) and lead to toxicity. Here, we sought to understand the effect that excessive shRNAs have on muscle miRNAs by treatin...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.12.007

    authors： Course MM,Gudsnuk K,Desai N,Chamberlain JR,Valdmanis PN

    更新日期：2020-03-06 00:00:00

  • Thioflavin T Monitoring of Guanine Quadruplex Formation in the rs689-Dependent INS Intron 1.

    abstract：:The human proinsulin gene (INS) contains a thymine-to-adenine variant (rs689) located in the 3' splice site (3' ss) recognition motif of the first intron. The adenine at rs689 is strongly associated with type 1 diabetes. By weakening the polypyrimidine tract, the adenine allele reduces the efficiency of intron 1 splic...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.04.026

    authors： Lages A,Proud CG,Holloway JW,Vorechovsky I

    更新日期：2019-06-07 00:00:00

  • An innovative data analysis strategy for accurate next-generation sequencing detection of tumor mitochondrial DNA mutations.

    abstract：:Next-generation sequencing technology has been commonly applied to detect mitochondrial DNA (mtDNA) mutations, which are reported to be strongly associated with cancers. However, several key challenges still exist regarding bioinformatics analysis of mtDNA sequencing data that greatly affect the detection accuracy of ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.11.002

    authors： Guo S,Zhou K,Yuan Q,Su L,Liu Y,Ji X,Gu X,Guo X,Xing J

    更新日期：2020-11-11 00:00:00

  • Identifying Involvement of H19-miR-675-3p-IGF1R and H19-miR-200a-PDCD4 in Treating Pulmonary Hypertension with Melatonin.

    abstract：:Non-coding RNAs play an important role in the pathogenesis of pulmonary arterial hypertension (PAH). The aim of this study was to characterize the therapeutic role of melatonin as well as the underlying molecular mechanism (its effects on the expression of H19 and its downstream signaling pathways) in the treatment of...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.08.015

    authors： Wang R,Zhou S,Wu P,Li M,Ding X,Sun L,Xu X,Zhou X,Zhou L,Cao C,Fei G

    更新日期：2018-12-07 00:00:00

  • H19 Increases IL-17A/IL-23 Releases via Regulating VDR by Interacting with miR675-5p/miR22-5p in Ankylosing Spondylitis.

    abstract：:Long non-coding RNA (lncRNA) H19 is associated with inflammatory diseases, but the molecular mechanism of H19 in the inflammatory process of ankylosing spondylitis (AS) is unclear. Here, we investigated the role of H19 and its downstream molecules in the inflammation of AS by microarray analysis, qRT-PCR, western blot...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.11.025

    authors： Zhang X,Ji S,Cai G,Pan Z,Han R,Yuan Y,Xu S,Yang J,Hu X,Chen M,Wu M,Ma Y,Deng J,Gao X,Guan S,Xu S,Shuai Z,Laslett L,Pan F

    更新日期：2020-03-06 00:00:00

  • Mir-34: a new weapon against cancer?

    abstract：:The microRNA(miRNA)-34a is a key regulator of tumor suppression. It controls the expression of a plethora of target proteins involved in cell cycle, differentiation and apoptosis, and antagonizes processes that are necessary for basic cancer cell viability as well as cancer stemness, metastasis, and chemoresistance. I...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章,评审

    doi：10.1038/mtna.2014.47

    authors： Misso G,Di Martino MT,De Rosa G,Farooqi AA,Lombardi A,Campani V,Zarone MR,Gullà A,Tagliaferri P,Tassone P,Caraglia M

    更新日期：2014-09-23 00:00:00

  • LncRNA LINC00963 Promotes Tumorigenesis and Radioresistance in Breast Cancer by Sponging miR-324-3p and Inducing ACK1 Expression.

    abstract：:Upregulation of long non-coding RNA LINC00963 has been observed in several cancer types. In this study, we analyzed the clinical and biological significance of LINC00963 in breast cancer. The key microRNA (miR) mediating the action of LINC00963 was identified. We show that LINC00963 upregulation is correlated with agg...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.09.033

    authors： Zhang N,Zeng X,Sun C,Guo H,Wang T,Wei L,Zhang Y,Zhao J,Ma X

    更新日期：2019-12-06 00:00:00

  • Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C>T and c.639+919G>A GLA Deep Intronic Mutations.

    abstract：:Fabry disease is a rare X-linked lysosomal storage disorder caused by deficiency of the α-galactosidase A (α-Gal A) enzyme, which is encoded by the GLA gene. GLA transcription in humans produces a major mRNA encoding α-Gal A and a minor mRNA of unknown function, which retains a 57-nucleotide-long cryptic exon between ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2016.88

    authors： Ferri L,Covello G,Caciotti A,Guerrini R,Denti MA,Morrone A

    更新日期：2016-10-25 00:00:00

  • Targeting the PD-1/PD-L1 Immune Evasion Axis With DNA Aptamers as a Novel Therapeutic Strategy for the Treatment of Disseminated Cancers.

    abstract：:Blocking the immunoinhibitory PD-1:PD-L1 pathway using monoclonal antibodies has led to dramatic clinical responses by reversing tumor immune evasion and provoking robust and durable antitumor responses. Anti-PD-1 antibodies have now been approved for the treatment of melanoma, and are being clinically tested in a num...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2015.11

    authors： Prodeus A,Abdul-Wahid A,Fischer NW,Huang EH,Cydzik M,Gariépy J

    更新日期：2015-04-28 00:00:00

  • Tat-haFGF14-154 Upregulates ADAM10 to Attenuate the Alzheimer Phenotype of APP/PS1 Mice through the PI3K-CREB-IRE1α/XBP1 Pathway.

    abstract：:Acid fibroblast growth factor (aFGF) has shown neuroprotection in Alzheimer's disease (AD) models in previous studies, yet its mechanism is still uncertain. Here we report that the efficacy of Tat-haFGF14-154 is markedly increased when loaded cationic liposomes for intranasal delivery are intranasally administered to ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2017.05.004

    authors： Meng T,Cao Q,Lei P,Bush AI,Xiang Q,Su Z,He X,Rogers JT,Chiu IM,Zhang Q,Huang Y

    更新日期：2017-06-16 00:00:00

  • Multi-pathway Protective Effects of MicroRNAs on Human Chondrocytes in an In Vitro Model of Osteoarthritis.

    abstract：:Osteoarthritis (OA) is the most common degenerative joint disease. One of the main pathogenic factors of OA is thought to be inflammation. Other factors associated with OA are dysregulation of microRNAs, reduced autophagic activity, oxidative stress, and altered metabolism. microRNAs are small non-coding RNAs that are...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.07.011

    authors： Al-Modawi RN,Brinchmann JE,Karlsen TA

    更新日期：2019-09-06 00:00:00

  • The miR-93-3p/ZFP36L1/ZFX axis regulates keratinocyte proliferation and migration during skin wound healing.

    abstract：:Keratinocyte proliferation and migration are crucial steps during skin wound healing. The functional role of microRNAs (miRs) remains relatively unknown during this process. miR-93 levels have been reported to increase within 24 h of skin wound healing; however, whether miR-93-3p or miR-93-5p plays a specific role in ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.11.017

    authors： Feng X,Zhou S,Cai W,Guo J

    更新日期：2020-11-26 00:00:00

  • Priming Human Repopulating Hematopoietic Stem and Progenitor Cells for Cas9/sgRNA Gene Targeting.

    abstract：:Engineered nuclease-mediated gene targeting through homologous recombination (HR) in hematopoietic stem and progenitor cells (HSPCs) has the potential to treat a variety of genetic hematologic and immunologic disorders. Here, we identify critical parameters to reproducibly achieve high frequencies of RNA-guided (singl...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.04.017

    authors： Charlesworth CT,Camarena J,Cromer MK,Vaidyanathan S,Bak RO,Carte JM,Potter J,Dever DP,Porteus MH

    更新日期：2018-09-07 00:00:00

  • circHMCU Promotes Proliferation and Metastasis of Breast Cancer by Sponging the let-7 Family.

    abstract：:Circular RNA (circRNA), as a kind of novel identified non-coding RNA, has become the focus of attention for its vital physiological and pathological roles. However, the function and mechanism of circRNAs in the regulation of cancer progression are largely unknown. In the present study we found a circRNA termed circHMC...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.03.014

    authors： Song X,Liang Y,Sang Y,Li Y,Zhang H,Chen B,Du L,Liu Y,Wang L,Zhao W,Ma T,Wang C,Yang Q

    更新日期：2020-06-05 00:00:00

  • Expression of Herpes Simplex Virus Thymidine Kinase/Ganciclovir by RNA Trans-Splicing Induces Selective Killing of HIV-Producing Cells.

    abstract：:Antiviral strategies targeting hijacked cellular processes are less easily evaded by the virus than viral targets. If selective for viral functions, they can have a high therapeutic index. We used RNA trans-splicing to deliver the herpes simplex virus thymidine kinase-ganciclovir (HSV-tk/GCV) cell suicide system into ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2017.03.004

    authors： Ingemarsdotter CK,Poddar S,Mercier S,Patzel V,Lever AML

    更新日期：2017-06-16 00:00:00

  • Mathematical Modeling: A Tool for Optimization of Lipid Nanoparticle-Mediated Delivery of siRNA.

    abstract：:Lipid nanoparticles (LNPs) have been used to successfully deliver small interfering RNAs (siRNAs) to target cells in both preclinical and clinical studies and currently are the leading systems for in vivo delivery. Here, we propose the use of an ordinary differential equation (ODE)-based model as a tool for optimizing...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2017.04.003

    authors： Mihaila R,Ruhela D,Keough E,Cherkaev E,Chang S,Galinski B,Bartz R,Brown D,Howell B,Cunningham JJ

    更新日期：2017-06-16 00:00:00

  • MSC-Derived Exosomes Protect Vertebral Endplate Chondrocytes against Apoptosis and Calcification via the miR-31-5p/ATF6 Axis.

    abstract：:Apoptosis and calcification of endplate chondrocytes (EPCs) can exacerbate intervertebral disc degeneration (IVDD). Mesenchymal stem cell-derived exosomes (MSC-exosomes) are reported to have the therapeutic potential in IVDD. However, the effects and related mechanisms of MSC-exosomes on EPCs are still unclear. We aim...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.09.026

    authors： Xie L,Chen Z,Liu M,Huang W,Zou F,Ma X,Tao J,Guo J,Xia X,Lyu F,Wang H,Zheng C,Jiang J

    更新日期：2020-09-26 00:00:00

  • miR-223 Regulates Cell Proliferation and Invasion via Targeting PDS5B in Pancreatic Cancer Cells.

    abstract：:Emerging evidence has demonstrated that miR-223 is critically involved in the progression of pancreatic cancer (PC); however, the underlying mechanisms are not fully elucidated. In the present study, we explored the molecular basis of miR-223-mediated tumor progression in PC. We performed numerous approaches including...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.01.009

    authors： Ma J,Cao T,Cui Y,Zhang F,Shi Y,Xia J,Wang ZP

    更新日期：2019-03-01 00:00:00

  • circINSR Promotes Proliferation and Reduces Apoptosis of Embryonic Myoblasts by Sponging miR-34a.

    abstract：:As a diverse and abundant class of endogenous RNAs, circular RNAs (circRNAs) participate in processes including cell proliferation and apoptosis. Nevertheless, few researchers have investigated the function of circRNAs in bovine muscle development. Based on existing sequencing data, we identified circINSR. The localiz...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.12.032

    authors： Shen X,Zhang X,Ru W,Huang Y,Lan X,Lei C,Chen H

    更新日期：2020-03-06 00:00:00

  • Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor.

    abstract：:Thanks to their ability to recognize biomolecular targets with high affinity and specificity, nucleic acid aptamers are increasingly investigated as diagnostic and therapeutic tools, particularly when their targets are cell-surface receptors. Here, we investigate the relationship between the folding of an anti-mouse t...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2013.71

    authors： Porciani D,Signore G,Marchetti L,Mereghetti P,Nifosì R,Beltram F

    更新日期：2014-01-28 00:00:00