当前位置： SCI文献检索 > Molecular Therapy-Nucleic Acids期刊下所有文献 > Triple bioluminescence imaging for in vivo monitoring of cellular processes.

Triple bioluminescence imaging for in vivo monitoring of cellular processes.

Abstract：

:Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability of other efficiently expressed luciferases using different substrates. Here, we characterized a codon-optimized luciferase from Vargula hilgendorfii (Vluc) as a reporter for mammalian gene expression. We showed that Vluc can be multiplexed with Gluc and Fluc for sequential imaging of three distinct cellular phenomena in the same biological system using vargulin, coelenterazine, and D-luciferin substrates, respectively. We applied this triple imaging system to monitor the effect of soluble tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL) delivered using an adeno-associated viral vector (AAV) on brain tumors in mice. Vluc imaging showed efficient sTRAIL gene delivery to the brain, while Fluc imaging revealed a robust antiglioma therapy. Further, nuclear factor-κB (NF-κB) activation in response to sTRAIL binding to glioma cells death receptors was monitored by Gluc imaging. This work is the first demonstration of trimodal in vivo bioluminescence imaging and will have a broad applicability in many different fields including immunology, oncology, virology, and neuroscience.Molecular Therapy - Nucleic Acids (2013) 2, e99; doi:10.1038/mtna.2013.25; published online 18 June 2013.

journal_name

Mol Ther Nucleic Acids

journal_title

Molecular therapy. Nucleic acids

authors

Maguire CA,Bovenberg MS,Crommentuijn MH,Niers JM,Kerami M,Teng J,Sena-Esteves M,Badr CE,Tannous BA

doi

10.1038/mtna.2013.25

subject

Has Abstract

pub_date

2013-06-18 00:00:00

pages

e99

issn

2162-2531

pii

mtna201325

journal_volume

2

pub_type

杂志文章

相关文献

Molecular Therapy-Nucleic Acids文献大全
  • lncRNA IGF2 AS Regulates Bovine Myogenesis through Different Pathways.

    abstract：:The role of long non-coding RNA (lncRNA) in the regulation of bovine skeletal muscle development remains poorly understood. The present study investigated the function and regulatory mechanism of a novel lncRNA, insulin-like growth factor 2 antisense transcript (IGF2 AS), in bovine myoblast proliferation and different...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.07.002

    authors： Song C,Yang Z,Jiang R,Cheng J,Yue B,Wang J,Sun X,Huang Y,Lan X,Lei C,Chen H

    更新日期：2020-09-04 00:00:00

  • Overexpressed lncRNA GATA6-AS1 Inhibits LNM and EMT via FZD4 through the Wnt/β-Catenin Signaling Pathway in GC.

    abstract：:Gastric cancer (GC) is one of the leading causes of cancer-related deaths worldwide. Accumulating evidence reveals the significance of long non-coding RNAs (lncRNAs) in various cancers. The current study aimed to evaluate the role of GATA6 antisense RNA 1 (GATA6-AS1) in the epithelial-mesenchymal transition (EMT) and ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.09.034

    authors： Li ZT,Zhang X,Wang DW,Xu J,Kou KJ,Wang ZW,Yong G,Liang DS,Sun XY

    更新日期：2020-03-06 00:00:00

  • Inhibition of Influenza Virus Replication by DNA Aptamers Targeting a Cellular Component of Translation Initiation.

    abstract：:The genetic diversity of the influenza virus hinders the use of broad spectrum antiviral drugs and favors the appearance of resistant strains. Single-stranded DNA aptamers represent an innovative approach with potential application as antiviral compounds. The mRNAs of influenza virus possess a 5'cap structure and a 3'...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2016.20

    authors： Rodriguez P,Pérez-Morgado MI,Gonzalez VM,Martín ME,Nieto A

    更新日期：2016-04-12 00:00:00

  • MicroRNA let-7i Inhibits Histone Lysine Demethylase KDM5B to Halt Esophageal Cancer Progression.

    abstract：:Recent studies have suggested that microRNA let-7i is a tumor suppressor in human cancers, including esophageal cancer, but its underlying mechanism is not yet fully understood. We investigated the role and mechanisms of let-7i in the progression of esophageal cancer. We first showed that let-7i was downregulated in e...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.09.012

    authors： Yang Y,Li W,Wei B,Wu K,Liu D,Zhu D,Zhang C,Wen F,Fan Y,Zhao S

    更新日期：2020-09-16 00:00:00

  • Identifying Involvement of H19-miR-675-3p-IGF1R and H19-miR-200a-PDCD4 in Treating Pulmonary Hypertension with Melatonin.

    abstract：:Non-coding RNAs play an important role in the pathogenesis of pulmonary arterial hypertension (PAH). The aim of this study was to characterize the therapeutic role of melatonin as well as the underlying molecular mechanism (its effects on the expression of H19 and its downstream signaling pathways) in the treatment of...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.08.015

    authors： Wang R,Zhou S,Wu P,Li M,Ding X,Sun L,Xu X,Zhou X,Zhou L,Cao C,Fei G

    更新日期：2018-12-07 00:00:00

  • Inhibition of Murine Cytomegalovirus Infection in Animals by RNase P-Associated External Guide Sequences.

    abstract：:External guide sequence (EGS) RNAs are associated with ribonuclease P (RNase P), a tRNA processing enzyme, and represent promising agents for gene-targeting applications as they can direct RNase-P-mediated cleavage of a target mRNA. Using murine cytomegalovirus (MCMV) as a model system, we examined the antiviral effec...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2017.10.007

    authors： Li W,Sheng J,Xu M,Vu GP,Yang Z,Liu Y,Sun X,Trang P,Lu S,Liu F

    更新日期：2017-12-15 00:00:00

  • Neutrophils are Essential in Short Hairpin RNA of Indoleamine 2,3- Dioxygenase Mediated-antitumor Efficiency.

    abstract：:Indoleamine 2,3-dioxygenase (IDO) is a rate limiting enzyme in tryptophan-degrading pathways and IDO activity results in immune suppression. Targeting IDO is a strategy of cancer immunotherapies. Our previous studies demonstrate that delivery of short hairpin against IDO (IDO shRNA) suppresses tumor growth and increas...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2016.105

    authors： Liu KT,Liu YH,Liu HL,Chong IW,Yen MC,Kuo PL

    更新日期：2016-12-06 00:00:00

  • Macrophage-Derived miRNA-Containing Exosomes Induce Peritendinous Fibrosis after Tendon Injury through the miR-21-5p/Smad7 Pathway.

    abstract：:Following tendon injury, the development of fibrotic healing response impairs tendon function and restricts tendon motion. Peritendinous tissue fibrosis poses a major clinical problem in hand surgery. Communication between macrophages and tendon cells has a critical role in regulating the tendon-healing process. Yet, ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.11.006

    authors： Cui H,He Y,Chen S,Zhang D,Yu Y,Fan C

    更新日期：2019-03-01 00:00:00

  • A short antisense oligonucleotide ameliorates symptoms of severe mouse models of spinal muscular atrophy.

    abstract：:Recent reports underscore the unparalleled potential of antisense-oligonucleotide (ASO)-based approaches to ameliorate various pathological conditions. However, in vivo studies validating the effectiveness of a short ASO (<10-mer) in the context of a human disease have not been performed. One disease with proven amena...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2014.23

    authors： Keil JM,Seo J,Howell MD,Hsu WH,Singh RN,DiDonato CJ

    更新日期：2014-07-08 00:00:00

  • One-Step Biallelic and Scarless Correction of a β-Thalassemia Mutation in Patient-Specific iPSCs without Drug Selection.

    abstract：:Monogenic disorders (MGDs), which are caused by single gene mutations, have a serious effect on human health. Among these, β-thalassemia (β-thal) represents one of the most common hereditary hematological diseases caused by mutations in the human hemoglobin β (HBB) gene. The technologies of induced pluripotent stem ce...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2016.11.010

    authors： Liu Y,Yang Y,Kang X,Lin B,Yu Q,Song B,Gao G,Chen Y,Sun X,Li X,Bu L,Fan Y

    更新日期：2017-03-17 00:00:00

  • Characterization of Transcriptome Transition Associates Long Noncoding RNAs with Glioma Progression.

    abstract：:Long noncoding RNAs (lncRNAs) have been implicated in cancer biogenesis and prognosis. However, we still lack knowledge on their function during glioma progression. In this study, we analyzed the lncRNA expression profile across 907 glioma patients in grades II, III, and IV. Widespread dynamic expression of lncRNAs du...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.10.009

    authors： Lin X,Jiang T,Bai J,Li J,Wang T,Xiao J,Tian Y,Jin X,Shao T,Xu J,Chen L,Wang L,Li Y

    更新日期：2018-12-07 00:00:00

  • H19 Increases IL-17A/IL-23 Releases via Regulating VDR by Interacting with miR675-5p/miR22-5p in Ankylosing Spondylitis.

    abstract：:Long non-coding RNA (lncRNA) H19 is associated with inflammatory diseases, but the molecular mechanism of H19 in the inflammatory process of ankylosing spondylitis (AS) is unclear. Here, we investigated the role of H19 and its downstream molecules in the inflammation of AS by microarray analysis, qRT-PCR, western blot...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.11.025

    authors： Zhang X,Ji S,Cai G,Pan Z,Han R,Yuan Y,Xu S,Yang J,Hu X,Chen M,Wu M,Ma Y,Deng J,Gao X,Guan S,Xu S,Shuai Z,Laslett L,Pan F

    更新日期：2020-03-06 00:00:00

  • A cancer cell-activatable aptamer-reporter system for one-step assay of circulating tumor cells.

    abstract：:The current antibody-mediated numeration assays of circulating tumor cells (CTCs) require multiple steps and are time-consuming. To overcome these technical limitations, a cancer cell-activatable aptamer-reporter was formulated by conjugating a biomarker-specific aptamer sequence with paired fluorochrome-quencher mole...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2014.36

    authors： Zeng Z,Tung CH,Zu Y

    更新日期：2014-08-12 00:00:00

  • RNA-Seq Analysis of an Antisense Sequence Optimized for Exon Skipping in Duchenne Patients Reveals No Off-Target Effect.

    abstract：:Non-coding uridine-rich small nuclear RNAs (UsnRNAs) have emerged in recent years as effective tools for exon skipping for the treatment of Duchenne muscular dystrophy (DMD), a degenerative muscular genetic disorder. We recently showed the high capacity of a recombinant adeno-associated virus (rAAV)-U7snRNA vector to ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2017.12.008

    authors： Domenger C,Allais M,François V,Léger A,Lecomte E,Montus M,Servais L,Voit T,Moullier P,Audic Y,Le Guiner C

    更新日期：2018-03-02 00:00:00

  • Pip6-PMO, A New Generation of Peptide-oligonucleotide Conjugates With Improved Cardiac Exon Skipping Activity for DMD Treatment.

    abstract：:Antisense oligonucleotides (AOs) are currently the most promising therapeutic intervention for Duchenne muscular dystrophy (DMD). AOs modulate dystrophin pre-mRNA splicing, thereby specifically restoring the dystrophin reading frame and generating a truncated but semifunctional dystrophin protein. Challenges in the de...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2012.30

    authors： Betts C,Saleh AF,Arzumanov AA,Hammond SM,Godfrey C,Coursindel T,Gait MJ,Wood MJ

    更新日期：2012-08-14 00:00:00

  • Long noncoding RNA SMUL suppresses SMURF2 production-mediated muscle atrophy via nonsense-mediated mRNA decay.

    abstract：:As the world population grows, muscle atrophy leading to muscle wasting could become a bigger risk. Long noncoding RNAs (lncRNAs) are known to play important roles in muscle growth and muscle atrophy. Meanwhile, it has recently come to light that many putative small open reading frames (sORFs) are hidden in lncRNAs; h...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.12.003

    authors： Cai B,Li Z,Ma M,Zhang J,Kong S,Abdalla BA,Xu H,Jebessa E,Zhang X,Lawal RA,Nie Q

    更新日期：2020-12-10 00:00:00

  • LiPS-A3S, a human genomic site for robust expression of inserted transgenes.

    abstract：:Transgenesis of human pluripotent stem cells (hPSCs) can enable and empower a variety of studies in stem cell research, including lineage tracing and functional genetics studies. While in recent years much progress has been made in the development of tools for gene targeting, little attention has been given to the ide...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2016.99

    authors： Kotini AG,Sadelain M,Papapetrou EP

    更新日期：2016-11-29 00:00:00

  • Improved Performance of Anti-miRNA Oligonucleotides Using a Novel Non-Nucleotide Modifier.

    abstract：:Anti-microRNA oligonucleotides (AMOs) are steric blocking antisense reagents that inhibit microRNA (miRNA) function by hybridizing and repressing the activity of a mature miRNA. First generation AMOs employed 2'-O-Methyl RNA nucleotides (2'OMe) with phosphorothioate (PS) internucleotide linkages positioned at both end...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2013.46

    authors： Lennox KA,Owczarzy R,Thomas DM,Walder JA,Behlke MA

    更新日期：2013-08-27 00:00:00

  • N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool.

    abstract：:MicroRNAs (miRNAs or miRs) play crucial roles in biological and pathological processes. Some miRNAs also appear as promising biomarkers and therapeutic tools. However, the epitranscriptomic regulation of miRNAs is not yet fully elucidated in all of their fields of application. We report that adenosine methylation of m...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.08.010

    authors： Briand J,Sérandour AA,Nadaradjane A,Bougras-Cartron G,Heymann D,Ory B,Vallette FM,Cartron PF

    更新日期：2020-08-14 00:00:00

  • Development of Lentiviral Vectors Simultaneously Expressing Multiple siRNAs Against CCR5, vif and tat/rev Genes for an HIV-1 Gene Therapy Approach.

    abstract：:Gene therapy holds considerable promise for the functional cure of HIV-1 infection and, in this context, RNA interference (RNAi)-based approaches represent powerful strategies. Stable expression of small interfering RNAs (siRNAs) targeting HIV genes or cellular cofactors has the potential to render HIV-1 susceptible c...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2016.24

    authors： Spanevello F,Calistri A,Del Vecchio C,Mantelli B,Frasson C,Basso G,Palù G,Cavazzana M,Parolin C

    更新日期：2016-04-19 00:00:00

  • Circular RNA Expression Profiling and the Potential Role of hsa_circ_0089172 in Hashimoto's Thyroiditis via Sponging miR125a-3p.

    abstract：:Circular RNA (circRNA) is a novel subclass of noncoding-RNA molecules that participate in development and progression of a variety of human diseases via sponging microRNAs (miRNAs), but the role of circRNAs in Hashimoto's thyroiditis (HT) has not been defined. In this study, peripheral blood samples from five patients...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.05.004

    authors： Xiong S,Peng H,Ding X,Wang X,Wang L,Wu C,Wang S,Xu H,Liu Y

    更新日期：2019-09-06 00:00:00

  • An innovative data analysis strategy for accurate next-generation sequencing detection of tumor mitochondrial DNA mutations.

    abstract：:Next-generation sequencing technology has been commonly applied to detect mitochondrial DNA (mtDNA) mutations, which are reported to be strongly associated with cancers. However, several key challenges still exist regarding bioinformatics analysis of mtDNA sequencing data that greatly affect the detection accuracy of ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.11.002

    authors： Guo S,Zhou K,Yuan Q,Su L,Liu Y,Ji X,Gu X,Guo X,Xing J

    更新日期：2020-11-11 00:00:00

  • Development of a Facile Approach for Generating Chemically Modified CRISPR/Cas9 RNA.

    abstract：:The RNA-guided, modified type II prokaryotic CRISPR with CRISPR-associated proteins (CRISPR/Cas9) system represents a simple gene-editing platform with applications in biotechnology and also potentially as a therapeutic modality. The system requires a small guide RNA (sgRNA) and a catalytic Cas9 protein to induce non-...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.01.004

    authors： Scott T,Soemardy C,Morris KV

    更新日期：2020-03-06 00:00:00

  • The MicroRNA Biology of the Mammalian Nucleus.

    abstract：:MicroRNAs (miRNAs) are a class of genome-encoded small RNAs that are primarily considered to be post-transcriptional negative regulators of gene expression acting in the cytoplasm. Over a decade of research has focused on this canonical paradigm of miRNA function, with many success stories. Indeed, miRNAs have been id...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2014.40

    authors： Roberts TC

    更新日期：2014-08-19 00:00:00

  • Binding and Structural Properties of DNA Aptamers with VEGF-A-Mimic Activity.

    abstract：:Vascular endothelial growth factors (VEGFs) are hypoxia-inducible secreted proteins to promote angiogenesis, in which VEGF-A is an important molecule that binds and activates VEGF receptor-1 (VEGFR-1) and VEGFR-2. In this study, two DNA aptamers, Apt01 and Apt02, were successfully isolated by alternating consecutive s...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.12.034

    authors： Yoshitomi T,Hayashi M,Oguro T,Kimura K,Wayama F,Furusho H,Yoshimoto K

    更新日期：2020-03-06 00:00:00

  • LncRNA TRERNA1 Function as an Enhancer of SNAI1 Promotes Gastric Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition.

    abstract：:Long noncoding RNA (lncRNA) has been implicated in cancer, but little is known about the role of lncRNAs as regulators of tumor metastasis. In the present study, we demonstrate that lncRNA TRERNA1 acts like an enhancer of SNAI1 to promote cell invasion and migration and to contribute to metastasis of gastric cancer (G...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2017.06.021

    authors： Wu H,Hu Y,Liu X,Song W,Gong P,Zhang K,Chen Z,Zhou M,Shen X,Qian Y,Fan H

    更新日期：2017-09-15 00:00:00

  • Dual Regulation of miR-34a and Notch Signaling in Triple-Negative Breast Cancer by Antibody/miRNA Nanocarriers.

    abstract：:Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer that lacks expression of the three most common receptors present on other subtypes, leaving it unsusceptible to current targeted or hormonal therapies. In this study, we introduce an alternative treatment strategy for TNBC that exploits its...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.06.003

    authors： Valcourt DM,Day ES

    更新日期：2020-09-04 00:00:00

  • Preclinical Development of a Subcutaneous ALAS1 RNAi Therapeutic for Treatment of Hepatic Porphyrias Using Circulating RNA Quantification.

    abstract：:The acute hepatic porphyrias are caused by inherited enzymatic deficiencies in the heme biosynthesis pathway. Induction of the first enzyme 5-aminolevulinic acid synthase 1 (ALAS1) by triggers such as fasting or drug exposure can lead to accumulation of neurotoxic heme intermediates that cause disease symptoms. We hav...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2015.36

    authors： Chan A,Liebow A,Yasuda M,Gan L,Racie T,Maier M,Kuchimanchi S,Foster D,Milstein S,Charisse K,Sehgal A,Manoharan M,Meyers R,Fitzgerald K,Simon A,Desnick RJ,Querbes W

    更新日期：2015-11-03 00:00:00

  • Inhibition of p66Shc Oxidative Signaling via CA-Induced Upregulation of miR-203a-3p Alleviates Liver Fibrosis Progression.

    abstract：:We previously found that inhibition of p66Shc confers protection against hepatic stellate cell (HSC) activation during liver fibrosis. However, the effect of p66Shc on HSC proliferation, as well as the mechanism by which p66Shc is modulated, remains unknown. Here, we elucidated the effect of p66Shc on HSC proliferatio...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.07.013

    authors： Wang Z,Zhao Y,Zhao H,Zhou J,Feng D,Tang F,Li Y,Lv L,Chen Z,Ma X,Tian X,Yao J

    更新日期：2020-09-04 00:00:00

  • Thioflavin T Monitoring of Guanine Quadruplex Formation in the rs689-Dependent INS Intron 1.

    abstract：:The human proinsulin gene (INS) contains a thymine-to-adenine variant (rs689) located in the 3' splice site (3' ss) recognition motif of the first intron. The adenine at rs689 is strongly associated with type 1 diabetes. By weakening the polypyrimidine tract, the adenine allele reduces the efficiency of intron 1 splic...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.04.026

    authors： Lages A,Proud CG,Holloway JW,Vorechovsky I

    更新日期：2019-06-07 00:00:00