N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool.

abstract：:Non-coding RNAs play an important role in the pathogenesis of pulmonary arterial hypertension (PAH). The aim of this study was to characterize the therapeutic role of melatonin as well as the underlying molecular mechanism (its effects on the expression of H19 and its downstream signaling pathways) in the treatment of...

journal_title：Molecular therapy. Nucleic acids

authors： Wang R,Zhou S,Wu P,Li M,Ding X,Sun L,Xu X,Zhou X,Zhou L,Cao C,Fei G

更新日期：2018-12-07 00:00:00

abstract：:Gene therapy holds considerable promise for the functional cure of HIV-1 infection and, in this context, RNA interference (RNAi)-based approaches represent powerful strategies. Stable expression of small interfering RNAs (siRNAs) targeting HIV genes or cellular cofactors has the potential to render HIV-1 susceptible c...

journal_title：Molecular therapy. Nucleic acids

authors： Spanevello F,Calistri A,Del Vecchio C,Mantelli B,Frasson C,Basso G,Palù G,Cavazzana M,Parolin C

更新日期：2016-04-19 00:00:00

abstract：:E-selectin is an adhesion molecule expressed on the luminal surface of inflamed blood vessels that mediates hematogenous metastasis by assisting shear-resistant adhesion of circulating tumor cells to the vessel surface under dynamic blood flow. Previously, we developed an E-selectin antagonistic thioaptamer (ESTA) for...

journal_title：Molecular therapy. Nucleic acids

authors： Morita Y,Kamal M,Kang SA,Zhang R,Lokesh GL,Thiviyanathan V,Hasan N,Woo S,Zhao D,Leslie M,Suh S,Razaq W,Rui H,Gorenstein DG,Volk DE,Tanaka T

更新日期：2016-12-13 00:00:00

abstract：:A gene therapeutic platform needs to be both efficient and safe. The criterion of safety is particularly important for diseases like hepatocellular carcinoma (HCC), which develop in a background of an already compromised liver. Gene vectors can be constructed either by targeting HCC or by detargeting liver and/or othe...

journal_title：Molecular therapy. Nucleic acids

authors： Dhungel B,Ramlogan-Steel CA,Layton CJ,Steel JC

更新日期：2018-12-07 00:00:00

abstract：:Glioblastoma is the most common malignant primary brain tumor among adults and one of the most lethal cancers. It is characterized by the deregulation of signaling pathways involving proliferation, growth, survival, and other factors. MicroRNAs (miRNAs) play a role in the regulation of genes by affecting the 3' untran...

journal_title：Molecular therapy. Nucleic acids

authors： Moradimotlagh A,Arefian E,Rezazadeh Valojerdi R,Ghaemi S,Jamshidi Adegani F,Soleimani M

更新日期：2020-03-06 00:00:00

abstract：:Circular RNA (circRNA), as a kind of novel identified non-coding RNA, has become the focus of attention for its vital physiological and pathological roles. However, the function and mechanism of circRNAs in the regulation of cancer progression are largely unknown. In the present study we found a circRNA termed circHMC...

journal_title：Molecular therapy. Nucleic acids

authors： Song X,Liang Y,Sang Y,Li Y,Zhang H,Chen B,Du L,Liu Y,Wang L,Zhao W,Ma T,Wang C,Yang Q

更新日期：2020-06-05 00:00:00

abstract：:The current antibody-mediated numeration assays of circulating tumor cells (CTCs) require multiple steps and are time-consuming. To overcome these technical limitations, a cancer cell-activatable aptamer-reporter was formulated by conjugating a biomarker-specific aptamer sequence with paired fluorochrome-quencher mole...

journal_title：Molecular therapy. Nucleic acids

authors： Zeng Z,Tung CH,Zu Y

更新日期：2014-08-12 00:00:00

abstract：:Mitochondrial DNA (mtDNA) haplogroups are associated with various types of cancer; however, the molecular mechanisms by which mtDNA haplogroups affect primary hepatocellular carcinoma (HCC) are not known. In this study, we carried out a case-control study on 388 HCC patients and 511 geographically matched asymptomatic...

journal_title：Molecular therapy. Nucleic acids

authors： Hua S,Li M,Zhao Q,Wang J,Zhou Y,Liu J,Fang H,Jiang M,Shen L

更新日期：2019-12-06 00:00:00

abstract：:We have reported that of the 10 most commonly used adeno-associated virus (AAV) serotype vectors, AAV6 is the most efficient in transducing primary human hematopoietic stem cells (HSCs) in vitro, as well as in vivo. More recently, polyvinyl alcohol (PVA), was reported to be a superior replacement for human serum album...

journal_title：Molecular therapy. Nucleic acids

authors： Yang H,Qing K,Keeler GD,Yin L,Mietzsch M,Ling C,Hoffman BE,Agbandje-McKenna M,Tan M,Wang W,Srivastava A

更新日期：2020-06-05 00:00:00

abstract：:Abnormal synthesis of extracellular matrix (ECM), especially collagen type IV (Col IV), in human retinal capillary endothelial cells (HRCECs) and resultant basement membrane (BM) thickening is the most prominent and characteristic feature of early diabetic retinopathy (DR). Osteopontin (OPN) has been shown to play an ...

journal_title：Molecular therapy. Nucleic acids

authors： Duan P,Chen S,Zeng Y,Xu H,Liu Y

更新日期：2020-06-05 00:00:00

abstract：:The genetic diversity of the influenza virus hinders the use of broad spectrum antiviral drugs and favors the appearance of resistant strains. Single-stranded DNA aptamers represent an innovative approach with potential application as antiviral compounds. The mRNAs of influenza virus possess a 5'cap structure and a 3'...

journal_title：Molecular therapy. Nucleic acids

authors： Rodriguez P,Pérez-Morgado MI,Gonzalez VM,Martín ME,Nieto A

更新日期：2016-04-12 00:00:00

abstract：:Ischemia-reperfusion (I/R) injury is a major side effect of the reperfusion treatment of the ischemic heart. Few therapies are available for the effective prevention of this injury caused by the oxidative stress-induced cardiomyocyte apoptosis. Metformin was shown to have a potential cardiac protective effect and abil...

journal_title：Molecular therapy. Nucleic acids

authors： Zhang Y,Liu X,Zhang L,Li X,Zhou Z,Jiao L,Shao Y,Li M,Leng B,Zhou Y,Liu T,Liu Q,Shan H,Du Z

更新日期：2018-12-07 00:00:00

abstract：:F-box and WD repeat domain-containing protein 7 (FBW7) has been documented to be implicated in nuclear factor κB (NF-κB) signaling and inflammation, but its role in the pathogenesis of inflammatory bowel disease (IBD) remains unknown. FBW7 was increased both in colon tissues from IBD patients and trinitrobenzene sulph...

journal_title：Molecular therapy. Nucleic acids

authors： Meng Q,Wu W,Pei T,Xue J,Xiao P,Sun L,Li L,Liang D

更新日期：2020-03-06 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is one of the most lethal human malignancies. Chemotherapeutic agents, such as sorafenib and lenvatinib, can improve the outcomes of HCC patients. Nevertheless, chemoresistance has become a major hurdle in the effective treatment of HCC. Noncoding RNAs (ncRNAs), including mircoRNAs (miRN...

journal_title：Molecular therapy. Nucleic acids

authors： Wang M,Yu F,Chen X,Li P,Wang K

更新日期：2020-09-04 00:00:00

abstract：:Oral squamous cell carcinoma (OSCC) is a highly recurrent form of cancer arising from the oral epithelium, which is the result of mutational change due to etiological factors such as tobacco, smoking, chewing of areca nuts, and alcohol consumption. OSCC occurrence has been observed to be prevalent in different regions...

journal_title：Molecular therapy. Nucleic acids

authors： Saikishore R,Velmurugan P,Ranjithkumar D,Latha R,Sathiamoorthi T,Arun A,Ravi AV,Sivakumar S

更新日期：2020-09-06 00:00:00

abstract：:Malignant glioma is a severe cancer with a poor prognosis. Local occurrence and rare metastases of malignant glioma make it a suitable target for gene therapy. Several studies have demonstrated the importance of Src kinase in different cancers. However, these studies have focused mainly on Src-deficient mice or pharma...

journal_title：Molecular therapy. Nucleic acids

authors： Stedt H,Alasaarela L,Samaranayake H,Pikkarainen J,Määttä AM,Kholová I,Parker AS,Ylä-Herttuala S

更新日期：2012-05-01 00:00:00

abstract：:Retinoic acid-inducible gene-I (RIG-I) is a cytosolic pathogen sensor that is crucial against a number of viral infections. Many viruses have evolved to inhibit pathogen sensors to suppress host innate immune responses. In the case of influenza, nonstructural protein 1 (NS1) suppresses RIG-I function, leading to viral...

journal_title：Molecular therapy. Nucleic acids

authors： Singh N,Ranjan P,Cao W,Patel J,Gangappa S,Davidson BA,Sullivan JM,Prasad PN,Knight PR,Sambhara S

更新日期：2020-03-06 00:00:00

abstract：:Limited duration of transgene expression, insertional mutagenesis, and size limitations for transgene cassettes pose challenges and risk factors for many gene therapy vectors. Here, we report on physiological expression of liver phenylalanine hydroxylase (PAH) by delivery of naked DNA/minicircle (MC)-based vectors for...

journal_title：Molecular therapy. Nucleic acids

authors： Grisch-Chan HM,Schlegel A,Scherer T,Allegri G,Heidelberger R,Tsikrika P,Schmeer M,Schleef M,Harding CO,Häberle J,Thöny B

更新日期：2017-06-16 00:00:00

abstract：:Acute lung injury (ALI) is a pulmonary disorder that causes acute respiratory failure, thus leading to relative high mortality worldwide. However, the molecular mechanisms of ALI remain largely unknown. MicroRNA (miRNA)-dependent control of gene expression at a post-transcriptional level has been recently reported. He...

journal_title：Molecular therapy. Nucleic acids

authors： Ying Y,Mao Y,Yao M

更新日期：2019-12-06 00:00:00

abstract：:Due to highly enzymatic d-stereoselectivity, l-nucleotides (l-2'-deoxynucleoside 5'-triphosphates [l-dNTPs]) are not natural targets of polymerases. In this study, we synthesized series of l-thymidine (l-T)-modified DNA strands and evaluated the processivity of nucleotide incorporation for transcription by T7 RNA poly...

journal_title：Molecular therapy. Nucleic acids

authors： Liu Q,Ke Y,Kan Y,Tang X,Li X,He Y,Wu L

更新日期：2020-09-04 00:00:00

abstract：:MicroRNAs (miRNAs) are small noncoding RNAs approximately 20-25 nt in length, which play crucial roles through directly binding to corresponding 3' UTR of targeted mRNAs. It has been reported that miRNAs are involved in numerous of diseases, including cancers. Recently, miR-134 has been identified to dysregulate in ha...

journal_title：Molecular therapy. Nucleic acids

authors： Pan JY,Zhang F,Sun CC,Li SJ,Li G,Gong FY,Bo T,He J,Hua RX,Hu WD,Yuan ZP,Wang X,He QQ,Li DJ

更新日期：2017-03-17 00:00:00

abstract：:Gene knockdown using short hairpin RNAs (shRNAs) is a promising strategy for targeting dominant mutations; however, delivering too much shRNA can disrupt the processing of endogenous microRNAs (miRNAs) and lead to toxicity. Here, we sought to understand the effect that excessive shRNAs have on muscle miRNAs by treatin...

journal_title：Molecular therapy. Nucleic acids

authors： Course MM,Gudsnuk K,Desai N,Chamberlain JR,Valdmanis PN

更新日期：2020-03-06 00:00:00

abstract：:Adeno-associated virus (AAV) is the most common vector for clinical gene therapy of the CNS. This popularity originates from a high safety record and the longevity of transgene expression in neurons. Nevertheless, clinical efficacy for CNS indications is lacking, and one reason for this is the relatively limited sprea...

journal_title：Molecular therapy. Nucleic acids

authors： Kanaan NM,Sellnow RC,Boye SL,Coberly B,Bennett A,Agbandje-McKenna M,Sortwell CE,Hauswirth WW,Boye SE,Manfredsson FP

更新日期：2017-09-15 00:00:00

abstract：:Neuroblastoma is the primary cause of cancer death in childhood. METTL14 is tightly linked to cancer. However, whether single-nucleotide polymorphisms (SNPs) in the METTL14 gene could predispose to neuroblastoma susceptibility lacks evidence. With an epidemiology case-control study, associations between METTL14 gene S...

journal_title：Molecular therapy. Nucleic acids

authors： Zhuo Z,Lu H,Zhu J,Hua RX,Li Y,Yang Z,Zhang J,Cheng J,Zhou H,Li S,Li L,Xia H,He J

更新日期：2020-08-14 00:00:00

abstract：:Recent studies have shown the effect of microRNAs on HSC activation and transformation, which is essential for the pathogenesis of liver fibrosis. In our study, we explored the role of miR-185 in liver fibrosis. Plasma miR-185 was detected in hepatitis B virus-related liver fibrosis patients (S2/3, n = 10) by Illumina...

journal_title：Molecular therapy. Nucleic acids

authors： Zhou L,Liu S,Han M,Ma Y,Feng S,Zhao J,Lu H,Yuan X,Cheng J

更新日期：2018-03-02 00:00:00

abstract：:Cardiac hypertrophy accompanied by maladaptive cardiac remodeling is the uppermost risk factor for the development of heart failure. Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have various biological functions, and their vital role in the regulation of cardiac hypertrophy still needs to be explored. In this...

journal_title：Molecular therapy. Nucleic acids

authors： Lv L,Li T,Li X,Xu C,Liu Q,Jiang H,Li Y,Liu Y,Yan H,Huang Q,Zhou Y,Zhang M,Shan H,Liang H

更新日期：2018-03-02 00:00:00

abstract：:Long noncoding RNA (lncRNA) has been implicated in cancer, but little is known about the role of lncRNAs as regulators of tumor metastasis. In the present study, we demonstrate that lncRNA TRERNA1 acts like an enhancer of SNAI1 to promote cell invasion and migration and to contribute to metastasis of gastric cancer (G...

journal_title：Molecular therapy. Nucleic acids

authors： Wu H,Hu Y,Liu X,Song W,Gong P,Zhang K,Chen Z,Zhou M,Shen X,Qian Y,Fan H

更新日期：2017-09-15 00:00:00

abstract：:A hexanucleotide GGGGCC expansion in intron 1 of chromosome 9 open reading frame 72 (C9orf72) gene is the most frequent cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The corresponding repeat-containing sense and antisense transcripts cause a gain of toxicity through the accumulation o...

journal_title：Molecular therapy. Nucleic acids

authors： Martier R,Liefhebber JM,García-Osta A,Miniarikova J,Cuadrado-Tejedor M,Espelosin M,Ursua S,Petry H,van Deventer SJ,Evers MM,Konstantinova P

更新日期：2019-06-07 00:00:00

abstract：:The microRNA(miRNA)-34a is a key regulator of tumor suppression. It controls the expression of a plethora of target proteins involved in cell cycle, differentiation and apoptosis, and antagonizes processes that are necessary for basic cancer cell viability as well as cancer stemness, metastasis, and chemoresistance. I...

journal_title：Molecular therapy. Nucleic acids

authors： Misso G,Di Martino MT,De Rosa G,Farooqi AA,Lombardi A,Campani V,Zarone MR,Gullà A,Tagliaferri P,Tassone P,Caraglia M

更新日期：2014-09-23 00:00:00

abstract：:Emerging evidence demonstrates that long non-coding RNAs (lncRNAs) are deeply involved in the development of various cancers. This study identified that SBF2-AS1, an early-stage-specific lncRNA, is critical for the tumorigenesis of lung adenocarcinoma (LUAD). We first analyzed LUAD transcriptome data from The Cancer G...

journal_title：Molecular therapy. Nucleic acids

authors： Chen R,Xia W,Wang S,Xu Y,Ma Z,Xu W,Zhang E,Wang J,Fang T,Zhang Q,Dong G,Cho WC,Ma PC,Brandi G,Tavolari S,Ujhazy P,Metro G,Popper HH,Yin R,Qiu M,Xu L

更新日期：2019-06-07 00:00:00