当前位置： SCI文献检索 > Molecular Therapy-Nucleic Acids期刊下所有文献 > Osteopontin Upregulates Col IV Expression by Repressing miR-29a in Human Retinal Capillary Endothelial Cells.

Osteopontin Upregulates Col IV Expression by Repressing miR-29a in Human Retinal Capillary Endothelial Cells.

Abstract：

:Abnormal synthesis of extracellular matrix (ECM), especially collagen type IV (Col IV), in human retinal capillary endothelial cells (HRCECs) and resultant basement membrane (BM) thickening is the most prominent and characteristic feature of early diabetic retinopathy (DR). Osteopontin (OPN) has been shown to play an important role in the pathogenesis of DR and specifically, found to be critically involved in diabetic nephropathy, as it can upregulate many factors, like collagen IV. However, the precise role of OPN in the pathogenesis of DR and the underlying mechanisms remain unclear. In this study, 51 differentially expressed microRNAs (miRNAs; 42 miRNAs upregulated and 9 miRNAs downregulated) were first identified in retina of streptozotocin (STZ)-induced diabetic mice with DR. Among these miRNAs, we identified miRNA (miR)-29a as a prominent miRNA that targeted and directly downregulated Col IV expression through database prediction and dual-luciferase reporter assay, which was further confirmed in HRCECs using miR-29a mimic, miR-29a inhibitor, and pre-miR-29a transfection. Furthermore, OPN upregulated Col IV expression via a miR-29a-repressed pathway in HRCECs. Taken together, these results provided a miR-29a-repressing mechanism through which OPN plays roles in abnormal synthesis of Col IV in HRCECs and resultant BM thickening, contributing to the pathogenesis of DR.

journal_name

Mol Ther Nucleic Acids

journal_title

Molecular therapy. Nucleic acids

authors

Duan P,Chen S,Zeng Y,Xu H,Liu Y

doi

10.1016/j.omtn.2020.02.001

subject

Has Abstract

pub_date

2020-06-05 00:00:00

pages

242-251

issn

2162-2531

pii

S2162-2531(20)30073-1

journal_volume

20

pub_type

杂志文章

相关文献

Molecular Therapy-Nucleic Acids文献大全
  • Overexpression of microRNA-203 Suppresses Proliferation, Invasion, and Migration while Accelerating Apoptosis of CSCC Cell Line SCL-1.

    abstract：:Cutaneous squamous cell carcinoma (CSCC) is a malignant proliferation of cutaneous epithelium that has been observed to have an alarming rise in incidence. Numerous studies have demonstrated microRNAs (miRNAs or miRs) as important biomarkers in the diagnosis, prognosis, and treatment of CSCC. This study aims to invest...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.04.014

    authors： Ting W,Feng C,Zhang M,Long F,Bai M

    更新日期：2020-09-04 00:00:00

  • ube3d, a New Gene Associated with Age-Related Macular Degeneration, Induces Functional Changes in Both In Vivo and In Vitro Studies.

    abstract：:Neovascular age-related macular degeneration (AMD) is characterized by the formation of choroidal neovascularization, which is responsible for more than 80% of cases of severe vision loss. Ubiquitin protein ligase E3D (UBE3D) gene missense has been proven to be associated with neovascular AMD in the East Asian populat...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.02.010

    authors： Xia H,Zhang Q,Shen Y,Bai Y,Ma X,Zhang B,Qi Y,Zhang J,Hu Q,Du W,Zhu L,Zhou P,Wang B,Xu H,Huang L,Li X

    更新日期：2020-06-05 00:00:00

  • Dose-Finding Study and Pharmacokinetics Profile of the Novel 13-Mer Antisense miR-221 Inhibitor in Sprague-Dawley Rats.

    abstract：:miR-221 is overexpressed in several malignancies where it promotes tumor growth and survival by interfering with gene transcripts, including p27Kip1, PUMA, PTEN, and p57Kip2. We previously demonstrated that a novel 13-mer miR-221 inhibitor (locked nucleic acid [LNA]-i-miR-221) exerts antitumor activity against human c...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.01.036

    authors： Di Martino MT,Arbitrio M,Caracciolo D,Scionti F,Tagliaferri P,Tassone P

    更新日期：2020-06-05 00:00:00

  • Mathematical Modeling: A Tool for Optimization of Lipid Nanoparticle-Mediated Delivery of siRNA.

    abstract：:Lipid nanoparticles (LNPs) have been used to successfully deliver small interfering RNAs (siRNAs) to target cells in both preclinical and clinical studies and currently are the leading systems for in vivo delivery. Here, we propose the use of an ordinary differential equation (ODE)-based model as a tool for optimizing...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2017.04.003

    authors： Mihaila R,Ruhela D,Keough E,Cherkaev E,Chang S,Galinski B,Bartz R,Brown D,Howell B,Cunningham JJ

    更新日期：2017-06-16 00:00:00

  • Specific inhibition of SRC kinase impairs malignant glioma growth in vitro and in vivo.

    abstract：:Malignant glioma is a severe cancer with a poor prognosis. Local occurrence and rare metastases of malignant glioma make it a suitable target for gene therapy. Several studies have demonstrated the importance of Src kinase in different cancers. However, these studies have focused mainly on Src-deficient mice or pharma...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2012.13

    authors： Stedt H,Alasaarela L,Samaranayake H,Pikkarainen J,Määttä AM,Kholová I,Parker AS,Ylä-Herttuala S

    更新日期：2012-05-01 00:00:00

  • Time-Restricted PiggyBac DNA Transposition by Transposase Protein Delivery Using Lentivirus-Derived Nanoparticles.

    abstract：:Continuous innovation of revolutionizing genome engineering technologies calls for an intensified focus on new delivery technologies that not only match the inventiveness of genome editors but also enable the combination of potent delivery and time-restricted action of genome-modifying bits and tools. We have previous...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.02.006

    authors： Skipper KA,Nielsen MG,Andersen S,Ryø LB,Bak RO,Mikkelsen JG

    更新日期：2018-06-01 00:00:00

  • CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells.

    abstract：:Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notabl...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章,评审

    doi：10.1038/mtna.2016.95

    authors： Wang X,Huang X,Fang X,Zhang Y,Wang W

    更新日期：2016-11-15 00:00:00

  • Targeting RNA-Mediated Toxicity in C9orf72 ALS and/or FTD by RNAi-Based Gene Therapy.

    abstract：:A hexanucleotide GGGGCC expansion in intron 1 of chromosome 9 open reading frame 72 (C9orf72) gene is the most frequent cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The corresponding repeat-containing sense and antisense transcripts cause a gain of toxicity through the accumulation o...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.02.001

    authors： Martier R,Liefhebber JM,García-Osta A,Miniarikova J,Cuadrado-Tejedor M,Espelosin M,Ursua S,Petry H,van Deventer SJ,Evers MM,Konstantinova P

    更新日期：2019-06-07 00:00:00

  • The Bipartite Network Projection-Recommended Algorithm for Predicting Long Non-coding RNA-Protein Interactions.

    abstract：:With the development of science and biotechnology, many evidences show that ncRNAs play an important role in the development of important biological processes, especially in chromatin modification, cell differentiation and proliferation, RNA progressing, human diseases, etc. Moreover, lncRNAs account for the majority ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.09.020

    authors： Zhao Q,Yu H,Ming Z,Hu H,Ren G,Liu H

    更新日期：2018-12-07 00:00:00

  • Targeted suicide gene therapy for liver cancer based on ribozyme-mediated RNA replacement through post-transcriptional regulation.

    abstract：:Hepatocellular carcinoma (HCC) has high fatality rate and limited therapeutic options. Here, we propose a new anti-HCC approach with high cancer-selectivity and efficient anticancer effects, based on adenovirus-mediated Tetrahymena group I trans-splicing ribozymes specifically inducing targeted suicide gene activity t...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.10.036

    authors： Han SR,Lee CH,Im JY,Kim JH,Kim JH,Kim SJ,Cho YW,Kim E,Kim Y,Ryu JH,Ju MH,Jeong JS,Lee SW

    更新日期：2020-10-31 00:00:00

  • Compatibility and Fidelity of Mirror-Image Thymidine in Transcription Events by T7 RNA Polymerase.

    abstract：:Due to highly enzymatic d-stereoselectivity, l-nucleotides (l-2'-deoxynucleoside 5'-triphosphates [l-dNTPs]) are not natural targets of polymerases. In this study, we synthesized series of l-thymidine (l-T)-modified DNA strands and evaluated the processivity of nucleotide incorporation for transcription by T7 RNA poly...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.06.023

    authors： Liu Q,Ke Y,Kan Y,Tang X,Li X,He Y,Wu L

    更新日期：2020-09-04 00:00:00

  • circINSR Promotes Proliferation and Reduces Apoptosis of Embryonic Myoblasts by Sponging miR-34a.

    abstract：:As a diverse and abundant class of endogenous RNAs, circular RNAs (circRNAs) participate in processes including cell proliferation and apoptosis. Nevertheless, few researchers have investigated the function of circRNAs in bovine muscle development. Based on existing sequencing data, we identified circINSR. The localiz...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.12.032

    authors： Shen X,Zhang X,Ru W,Huang Y,Lan X,Lei C,Chen H

    更新日期：2020-03-06 00:00:00

  • Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor.

    abstract：:Thanks to their ability to recognize biomolecular targets with high affinity and specificity, nucleic acid aptamers are increasingly investigated as diagnostic and therapeutic tools, particularly when their targets are cell-surface receptors. Here, we investigate the relationship between the folding of an anti-mouse t...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2013.71

    authors： Porciani D,Signore G,Marchetti L,Mereghetti P,Nifosì R,Beltram F

    更新日期：2014-01-28 00:00:00

  • Notch-1 Confers Chemoresistance in Lung Adenocarcinoma to Taxanes through AP-1/microRNA-451 Mediated Regulation of MDR-1.

    abstract：:We previously demonstrated that expression of Notch-1 is associated with poor prognosis in lung adenocarcinoma (LAD) patients. The aim of this study is to reveal whether Notch-1 was associated with Taxanes-resistant LAD and, the underlying mechanisms. We collected 39 patients of advanced LAD treated with Taxanes and f...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2016.82

    authors： Huang J,Chen Y,Li J,Zhang K,Chen J,Chen D,Feng B,Song H,Feng J,Wang R,Chen L

    更新日期：2016-10-11 00:00:00

  • miR-223 Regulates Cell Proliferation and Invasion via Targeting PDS5B in Pancreatic Cancer Cells.

    abstract：:Emerging evidence has demonstrated that miR-223 is critically involved in the progression of pancreatic cancer (PC); however, the underlying mechanisms are not fully elucidated. In the present study, we explored the molecular basis of miR-223-mediated tumor progression in PC. We performed numerous approaches including...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.01.009

    authors： Ma J,Cao T,Cui Y,Zhang F,Shi Y,Xia J,Wang ZP

    更新日期：2019-03-01 00:00:00

  • MicroRNA199a-Based Post-transcriptional Detargeting of Gene Vectors for Hepatocellular Carcinoma.

    abstract：:A gene therapeutic platform needs to be both efficient and safe. The criterion of safety is particularly important for diseases like hepatocellular carcinoma (HCC), which develop in a background of an already compromised liver. Gene vectors can be constructed either by targeting HCC or by detargeting liver and/or othe...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.08.016

    authors： Dhungel B,Ramlogan-Steel CA,Layton CJ,Steel JC

    更新日期：2018-12-07 00:00:00

  • PAK1 Promotes the Proliferation and Inhibits Apoptosis of Human Spermatogonial Stem Cells via PDK1/KDR/ZNF367 and ERK1/2 and AKT Pathways.

    abstract：:Spermatogonial stem cells (SSCs) have significant applications in reproductive and regenerative medicine. However, nothing is known about genes in mediating human SSCs. Here we have explored for the first time the function and mechanism of P21-activated kinase 1 (PAK1) in regulating the proliferation and apoptosis of ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.06.006

    authors： Fu H,Zhang W,Yuan Q,Niu M,Zhou F,Qiu Q,Mao G,Wang H,Wen L,Sun M,Li Z,He Z

    更新日期：2018-09-07 00:00:00

  • N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool.

    abstract：:MicroRNAs (miRNAs or miRs) play crucial roles in biological and pathological processes. Some miRNAs also appear as promising biomarkers and therapeutic tools. However, the epitranscriptomic regulation of miRNAs is not yet fully elucidated in all of their fields of application. We report that adenosine methylation of m...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.08.010

    authors： Briand J,Sérandour AA,Nadaradjane A,Bougras-Cartron G,Heymann D,Ory B,Vallette FM,Cartron PF

    更新日期：2020-08-14 00:00:00

  • The Circular RNome of Developmental Retina in Mice.

    abstract：:Circular RNAs (circRNAs) represent a class of noncoding RNAs with a wide expression pattern, and they constitute an important layer of the genome regulatory network. To date, the expression pattern and regulatory potency of circRNAs in the retina, a key part of the central nervous system, are not yet well understood. ...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.11.016

    authors： Chen XJ,Zhang ZC,Wang XY,Zhao HQ,Li ML,Ma Y,Ji YY,Zhang CJ,Wu KC,Xiang L,Sun LF,Zhou M,Jin ZB

    更新日期：2020-03-06 00:00:00

  • Inhibition of Influenza Virus Replication by DNA Aptamers Targeting a Cellular Component of Translation Initiation.

    abstract：:The genetic diversity of the influenza virus hinders the use of broad spectrum antiviral drugs and favors the appearance of resistant strains. Single-stranded DNA aptamers represent an innovative approach with potential application as antiviral compounds. The mRNAs of influenza virus possess a 5'cap structure and a 3'...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2016.20

    authors： Rodriguez P,Pérez-Morgado MI,Gonzalez VM,Martín ME,Nieto A

    更新日期：2016-04-12 00:00:00

  • Enhanced Tailored MicroRNA Sponge Activity of RNA Pol II-Transcribed TuD Hairpins Relative to Ectopically Expressed ciRS7-Derived circRNAs.

    abstract：:As key regulators of gene expression, microRNAs (miRNAs) have emerged as targets in basic experimentation and therapy. Administration of DNA-encoded RNA molecules, targeting miRNAs through base pairing, is one viable strategy for inhibiting specific miRNAs. A naturally occurring circular RNA (circRNA), ciRS-7, serving...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.09.009

    authors： Hollensen AK,Andersen S,Hjorth K,Bak RO,Hansen TB,Kjems J,Aagaard L,Damgaard CK,Mikkelsen JG

    更新日期：2018-12-07 00:00:00

  • Endogenous MicroRNA Competition as a Mechanism of shRNA-Induced Cardiotoxicity.

    abstract：:Gene knockdown using short hairpin RNAs (shRNAs) is a promising strategy for targeting dominant mutations; however, delivering too much shRNA can disrupt the processing of endogenous microRNAs (miRNAs) and lead to toxicity. Here, we sought to understand the effect that excessive shRNAs have on muscle miRNAs by treatin...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.12.007

    authors： Course MM,Gudsnuk K,Desai N,Chamberlain JR,Valdmanis PN

    更新日期：2020-03-06 00:00:00

  • H19 Increases IL-17A/IL-23 Releases via Regulating VDR by Interacting with miR675-5p/miR22-5p in Ankylosing Spondylitis.

    abstract：:Long non-coding RNA (lncRNA) H19 is associated with inflammatory diseases, but the molecular mechanism of H19 in the inflammatory process of ankylosing spondylitis (AS) is unclear. Here, we investigated the role of H19 and its downstream molecules in the inflammation of AS by microarray analysis, qRT-PCR, western blot...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.11.025

    authors： Zhang X,Ji S,Cai G,Pan Z,Han R,Yuan Y,Xu S,Yang J,Hu X,Chen M,Wu M,Ma Y,Deng J,Gao X,Guan S,Xu S,Shuai Z,Laslett L,Pan F

    更新日期：2020-03-06 00:00:00

  • Gemcitabine-Incorporated G-Quadruplex Aptamer for Targeted Drug Delivery into Pancreas Cancer.

    abstract：:Gemcitabine has been considered a first-line chemotherapy agent for the treatment of pancreatic cancer. However, the initial response rate of gemcitabine is low and chemoresistance occurs frequently. Aptamers can be effectively internalized into cancer cells via binding to target molecules with high affinity and speci...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.06.003

    authors： Park JY,Cho YL,Chae JR,Moon SH,Cho WG,Choi YJ,Lee SJ,Kang WJ

    更新日期：2018-09-07 00:00:00

  • Exosomes Derived from miR-143-Overexpressing MSCs Inhibit Cell Migration and Invasion in Human Prostate Cancer by Downregulating TFF3.

    abstract：:Exosomes are membrane-enclosed nanovesicles that shuttle active cargoes, such as mRNAs and microRNAs (miRNAs), between different cells. Mesenchymal stem cells (MSCs) are able to migrate to the tumor sites and exert complex functions over tumor progress. We investigated the effect of human bone marrow-derived MSC (BMSC...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2019.08.010

    authors： Che Y,Shi X,Shi Y,Jiang X,Ai Q,Shi Y,Gong F,Jiang W

    更新日期：2019-12-06 00:00:00

  • Preclinical in vivo evaluation of the safety of a multi-shRNA-based gene therapy against HIV-1.

    abstract：:Highly active antiretroviral therapy (HAART) has significantly improved the quality of life and the life expectancy of HIV-infected individuals. Still, drug-induced side effects and emergence of drug-resistant viral variants remain important issues that justify the exploration of alternative therapeutic options. One s...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2013.48

    authors： Centlivre M,Legrand N,Klamer S,Liu YP,Jasmijn von Eije K,Bohne M,Rijnstra ES,Weijer K,Blom B,Voermans C,Spits H,Berkhout B

    更新日期：2013-09-03 00:00:00

  • Circular RNA circTNPO3 Regulates Paclitaxel Resistance of Ovarian Cancer Cells by miR-1299/NEK2 Signaling Pathway.

    abstract：:Circular RNAs (circRNAs) were recently reported to be involved in the pathogenesis of ovarian cancer (OC); however, the molecular mechanisms of circRNAs in tumor progression and paclitaxel (PTX) resistance of OC remain largely undetermined. Here, we focused on circTNPO3 (hsa_circ_0001741), which is located on chromoso...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.06.002

    authors： Xia B,Zhao Z,Wu Y,Wang Y,Zhao Y,Wang J

    更新日期：2020-09-04 00:00:00

  • Generation of Genetically Stable Human Direct-Conversion-Derived Neural Stem Cells Using Quantity Control of Proto-oncogene Expression.

    abstract：:As the human lifespan has increased due to developments in medical technology, the number of patients with neurological diseases has rapidly increased. Therefore, studies on effective treatments for neurological diseases are becoming increasingly important. To perform these studies, it is essential to obtain a large n...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2018.12.009

    authors： Daekee K,Mi-Jung H,Minjun J,Hee-Jin A,Kwang-Won S,Kyung-Sun K

    更新日期：2019-03-01 00:00:00

  • Transcriptional Factor Yin Yang 1 Promotes the Stemness of Breast Cancer Cells by Suppressing miR-873-5p Transcriptional Activity.

    abstract：:Transcription factor Yin Yang 1 (YY1) is upregulated in multiple tumors and plays essential roles in tumor proliferation and metastasis. However, the function of YY1 in breast cancer stemness remains unclear. Herein, we found that YY1 expression was negatively correlated with the overall survival and relapse-free surv...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1016/j.omtn.2020.06.018

    authors： Guo Q,Wang T,Yang Y,Gao L,Zhao Q,Zhang W,Xi T,Zheng L

    更新日期：2020-09-04 00:00:00

  • Histone-targeted Polyplexes Avoid Endosomal Escape and Enter the Nucleus During Postmitotic Redistribution of ER Membranes.

    abstract：:Nonviral gene delivery is a promising therapeutic approach because of its safety and controllability, yet limited gene transfer efficacy is a common issue. Most nonviral strategies rely upon endosomal escape designs; however, endosomal escape is often uncorrelated with improved gene transfer and membranolytic structur...

    journal_title：Molecular therapy. Nucleic acids

    pub_type： 杂志文章

    doi：10.1038/mtna.2015.2

    authors： Ross NL,Munsell EV,Sabanayagam C,Sullivan MO

    更新日期：2015-02-10 00:00:00