lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.

abstract：:Current acute myeloid leukemia (AML) disease models face severe limitations because most of them induce un-physiological gene expressions that do not represent conditions in AML patients and/or depend on external promoters for regulation of gene expression/repression. Furthermore, many AML models are based on reciproc...

journal_title：Molecular therapy. Nucleic acids

authors： Brabetz O,Alla V,Angenendt L,Schliemann C,Berdel WE,Arteaga MF,Mikesch JH

更新日期：2017-03-17 00:00:00

abstract：:Mitochondrial DNA (mtDNA) haplogroups are associated with various types of cancer; however, the molecular mechanisms by which mtDNA haplogroups affect primary hepatocellular carcinoma (HCC) are not known. In this study, we carried out a case-control study on 388 HCC patients and 511 geographically matched asymptomatic...

journal_title：Molecular therapy. Nucleic acids

authors： Hua S,Li M,Zhao Q,Wang J,Zhou Y,Liu J,Fang H,Jiang M,Shen L

更新日期：2019-12-06 00:00:00

abstract：:Ischemia-reperfusion (I/R) injury is a major side effect of the reperfusion treatment of the ischemic heart. Few therapies are available for the effective prevention of this injury caused by the oxidative stress-induced cardiomyocyte apoptosis. Metformin was shown to have a potential cardiac protective effect and abil...

journal_title：Molecular therapy. Nucleic acids

authors： Zhang Y,Liu X,Zhang L,Li X,Zhou Z,Jiao L,Shao Y,Li M,Leng B,Zhou Y,Liu T,Liu Q,Shan H,Du Z

更新日期：2018-12-07 00:00:00

abstract：:Precise genome editing using engineered nucleases can significantly facilitate biological studies and disease treatment. In particular, clustered regularly interspaced short palindromic repeats (CRISPR) with CRISPR-associated (Cas) proteins are a potentially powerful tool for modifying a genome by targeted cleavage of...

journal_title：Molecular therapy. Nucleic acids

authors： Cradick TJ,Qiu P,Lee CM,Fine EJ,Bao G

更新日期：2014-12-02 00:00:00

abstract：:Next-generation sequencing technology has been commonly applied to detect mitochondrial DNA (mtDNA) mutations, which are reported to be strongly associated with cancers. However, several key challenges still exist regarding bioinformatics analysis of mtDNA sequencing data that greatly affect the detection accuracy of ...

journal_title：Molecular therapy. Nucleic acids

authors： Guo S,Zhou K,Yuan Q,Su L,Liu Y,Ji X,Gu X,Guo X,Xing J

更新日期：2020-11-11 00:00:00

abstract：:Spermatogonial stem cells (SSCs) have significant applications in reproductive and regenerative medicine. However, nothing is known about genes in mediating human SSCs. Here we have explored for the first time the function and mechanism of P21-activated kinase 1 (PAK1) in regulating the proliferation and apoptosis of ...

journal_title：Molecular therapy. Nucleic acids

authors： Fu H,Zhang W,Yuan Q,Niu M,Zhou F,Qiu Q,Mao G,Wang H,Wen L,Sun M,Li Z,He Z

更新日期：2018-09-07 00:00:00

abstract：:Besides the acquisition of pharmacokinetic parameters of antisense oligonucleotide microRNA (miRNA) inhibitors, such as measuring in vivo concentration, their pharmacodynamic characteristics are also of interest. An emerging and straightforward method for studying molecular interactions is microscale thermophoresis (M...

journal_title：Molecular therapy. Nucleic acids

authors： Herkt M,Batkai S,Thum T

更新日期：2019-12-06 00:00:00

abstract：:Long non-coding RNA (lncRNA) H19 is associated with inflammatory diseases, but the molecular mechanism of H19 in the inflammatory process of ankylosing spondylitis (AS) is unclear. Here, we investigated the role of H19 and its downstream molecules in the inflammation of AS by microarray analysis, qRT-PCR, western blot...

journal_title：Molecular therapy. Nucleic acids

authors： Zhang X,Ji S,Cai G,Pan Z,Han R,Yuan Y,Xu S,Yang J,Hu X,Chen M,Wu M,Ma Y,Deng J,Gao X,Guan S,Xu S,Shuai Z,Laslett L,Pan F

更新日期：2020-03-06 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is one of the most lethal human malignancies. Chemotherapeutic agents, such as sorafenib and lenvatinib, can improve the outcomes of HCC patients. Nevertheless, chemoresistance has become a major hurdle in the effective treatment of HCC. Noncoding RNAs (ncRNAs), including mircoRNAs (miRN...

journal_title：Molecular therapy. Nucleic acids

authors： Wang M,Yu F,Chen X,Li P,Wang K

更新日期：2020-09-04 00:00:00

abstract：:Limited duration of transgene expression, insertional mutagenesis, and size limitations for transgene cassettes pose challenges and risk factors for many gene therapy vectors. Here, we report on physiological expression of liver phenylalanine hydroxylase (PAH) by delivery of naked DNA/minicircle (MC)-based vectors for...

journal_title：Molecular therapy. Nucleic acids

authors： Grisch-Chan HM,Schlegel A,Scherer T,Allegri G,Heidelberger R,Tsikrika P,Schmeer M,Schleef M,Harding CO,Häberle J,Thöny B

更新日期：2017-06-16 00:00:00

abstract：:We previously found that inhibition of p66Shc confers protection against hepatic stellate cell (HSC) activation during liver fibrosis. However, the effect of p66Shc on HSC proliferation, as well as the mechanism by which p66Shc is modulated, remains unknown. Here, we elucidated the effect of p66Shc on HSC proliferatio...

journal_title：Molecular therapy. Nucleic acids

authors： Wang Z,Zhao Y,Zhao H,Zhou J,Feng D,Tang F,Li Y,Lv L,Chen Z,Ma X,Tian X,Yao J

更新日期：2020-09-04 00:00:00

abstract：:Keratinocyte proliferation and migration are crucial steps during skin wound healing. The functional role of microRNAs (miRs) remains relatively unknown during this process. miR-93 levels have been reported to increase within 24 h of skin wound healing; however, whether miR-93-3p or miR-93-5p plays a specific role in ...

journal_title：Molecular therapy. Nucleic acids

authors： Feng X,Zhou S,Cai W,Guo J

更新日期：2020-11-26 00:00:00

abstract：:Ribozyme genes were designed to reduce voluntary alcohol drinking in a rat model of alcohol dependence. Acetaldehyde generated from alcohol in the liver is metabolized by the mitochondrial aldehyde dehydrogenase (ALDH2) such that diminishing ALDH2 activity leads to the aversive effects of blood acetaldehyde upon alcoh...

journal_title：Molecular therapy. Nucleic acids

authors： Sapag A,Irrazábal T,Lobos-González L,Muñoz-Brauning CR,Quintanilla ME,Tampier L

更新日期：2016-07-12 00:00:00

abstract：:Oral squamous cell carcinoma (OSCC) is a highly recurrent form of cancer arising from the oral epithelium, which is the result of mutational change due to etiological factors such as tobacco, smoking, chewing of areca nuts, and alcohol consumption. OSCC occurrence has been observed to be prevalent in different regions...

journal_title：Molecular therapy. Nucleic acids

authors： Saikishore R,Velmurugan P,Ranjithkumar D,Latha R,Sathiamoorthi T,Arun A,Ravi AV,Sivakumar S

更新日期：2020-09-06 00:00:00

abstract：:Exosomes are membrane-enclosed nanovesicles that shuttle active cargoes, such as mRNAs and microRNAs (miRNAs), between different cells. Mesenchymal stem cells (MSCs) are able to migrate to the tumor sites and exert complex functions over tumor progress. We investigated the effect of human bone marrow-derived MSC (BMSC...

journal_title：Molecular therapy. Nucleic acids

authors： Che Y,Shi X,Shi Y,Jiang X,Ai Q,Shi Y,Gong F,Jiang W

更新日期：2019-12-06 00:00:00

abstract：:The role of microglia in the pathophysiology of ischemic retinal diseases has been studied extensively. Exosomes from microglial cells exert protective effects during several nervous system diseases, but their roles in hypoxia-induced retinopathy remain unclear. In our study, exosomes derived from microglial cells wer...

journal_title：Molecular therapy. Nucleic acids

authors： Xu W,Wu Y,Hu Z,Sun L,Dou G,Zhang Z,Wang H,Guo C,Wang Y

更新日期：2019-06-07 00:00:00

abstract：:Non-coding uridine-rich small nuclear RNAs (UsnRNAs) have emerged in recent years as effective tools for exon skipping for the treatment of Duchenne muscular dystrophy (DMD), a degenerative muscular genetic disorder. We recently showed the high capacity of a recombinant adeno-associated virus (rAAV)-U7snRNA vector to ...

journal_title：Molecular therapy. Nucleic acids

authors： Domenger C,Allais M,François V,Léger A,Lecomte E,Montus M,Servais L,Voit T,Moullier P,Audic Y,Le Guiner C

更新日期：2018-03-02 00:00:00

abstract：:Leukemias arise from genetic alterations in normal hematopoietic stem or progenitor cells, leading to impaired regulation of proliferation, differentiation, apoptosis, and survival of the transformed cells. With the advent of RNA interference (RNAi) and the short interfering RNA (siRNA) as its pharmacological mediator...

journal_title：Molecular therapy. Nucleic acids

authors： Landry B,Valencia-Serna J,Gul-Uludag H,Jiang X,Janowska-Wieczorek A,Brandwein J,Uludag H

更新日期：2015-05-12 00:00:00

abstract：:Due to highly enzymatic d-stereoselectivity, l-nucleotides (l-2'-deoxynucleoside 5'-triphosphates [l-dNTPs]) are not natural targets of polymerases. In this study, we synthesized series of l-thymidine (l-T)-modified DNA strands and evaluated the processivity of nucleotide incorporation for transcription by T7 RNA poly...

journal_title：Molecular therapy. Nucleic acids

authors： Liu Q,Ke Y,Kan Y,Tang X,Li X,He Y,Wu L

更新日期：2020-09-04 00:00:00

abstract：:With the development of science and biotechnology, many evidences show that ncRNAs play an important role in the development of important biological processes, especially in chromatin modification, cell differentiation and proliferation, RNA progressing, human diseases, etc. Moreover, lncRNAs account for the majority ...

journal_title：Molecular therapy. Nucleic acids

authors： Zhao Q,Yu H,Ming Z,Hu H,Ren G,Liu H

更新日期：2018-12-07 00:00:00

abstract：:Abnormal synthesis of extracellular matrix (ECM), especially collagen type IV (Col IV), in human retinal capillary endothelial cells (HRCECs) and resultant basement membrane (BM) thickening is the most prominent and characteristic feature of early diabetic retinopathy (DR). Osteopontin (OPN) has been shown to play an ...

journal_title：Molecular therapy. Nucleic acids

authors： Duan P,Chen S,Zeng Y,Xu H,Liu Y

更新日期：2020-06-05 00:00:00

abstract：:miR-221 is overexpressed in several malignancies where it promotes tumor growth and survival by interfering with gene transcripts, including p27Kip1, PUMA, PTEN, and p57Kip2. We previously demonstrated that a novel 13-mer miR-221 inhibitor (locked nucleic acid [LNA]-i-miR-221) exerts antitumor activity against human c...

journal_title：Molecular therapy. Nucleic acids

authors： Di Martino MT,Arbitrio M,Caracciolo D,Scionti F,Tagliaferri P,Tassone P

更新日期：2020-06-05 00:00:00

abstract：:Menin displays either tumor suppression or promotion functions in a context-dependent manner. Previously, we proposed that Menin acts as a tumor suppressor by inhibiting cell growth in pancreatic ductal adenocarcinoma (PDAC), whereas the relationship between the Menin expression and overall survival rate of PDAC patie...

journal_title：Molecular therapy. Nucleic acids

authors： Cheng P,Chen Y,He TL,Wang C,Guo SW,Hu H,Ni CM,Jin G,Zhang YJ

更新日期：2019-12-06 00:00:00

abstract：:The melanoma cell adhesion molecule (MCAM) is involved in melanoma development and its progression, including invasiveness, metastatic potential and angiogenesis. Therefore, MCAM represents a potential target for gene therapy of melanoma, whose expression could be hindered with posttranscriptional specific gene silenc...

journal_title：Molecular therapy. Nucleic acids

authors： Prosen L,Markelc B,Dolinsek T,Music B,Cemazar M,Sersa G

更新日期：2014-10-28 00:00:00

abstract：:Apoptosis and calcification of endplate chondrocytes (EPCs) can exacerbate intervertebral disc degeneration (IVDD). Mesenchymal stem cell-derived exosomes (MSC-exosomes) are reported to have the therapeutic potential in IVDD. However, the effects and related mechanisms of MSC-exosomes on EPCs are still unclear. We aim...

journal_title：Molecular therapy. Nucleic acids

authors： Xie L,Chen Z,Liu M,Huang W,Zou F,Ma X,Tao J,Guo J,Xia X,Lyu F,Wang H,Zheng C,Jiang J

更新日期：2020-09-26 00:00:00

abstract：:Nonviral vector systems are used increasingly in gene targeting and gene transfer applications. The piggyBac transposon represents an alternative integrating vector for in vivo gene transfer. We hypothesized that this system could achieve persistent gene transfer to the liver when administered systemically. We report ...

journal_title：Molecular therapy. Nucleic acids

authors： Burnight ER,Staber JM,Korsakov P,Li X,Brett BT,Scheetz TE,Craig NL,McCray PB Jr

更新日期：2012-10-16 00:00:00

abstract：:Retinoic acid-inducible gene-I (RIG-I) is a cytosolic pathogen sensor that is crucial against a number of viral infections. Many viruses have evolved to inhibit pathogen sensors to suppress host innate immune responses. In the case of influenza, nonstructural protein 1 (NS1) suppresses RIG-I function, leading to viral...

journal_title：Molecular therapy. Nucleic acids

authors： Singh N,Ranjan P,Cao W,Patel J,Gangappa S,Davidson BA,Sullivan JM,Prasad PN,Knight PR,Sambhara S

更新日期：2020-03-06 00:00:00

abstract：:Gene therapy, in its current configuration, is irreversible and does not allow control over transgene expression in case of side effects. Only few regulated vector systems are available, and none of these has reached clinical applicability yet. The mifepristone (Mfp)-regulated Gene Switch (GS) system is characterized ...

journal_title：Molecular therapy. Nucleic acids

authors： Maddalena A,Tereshchenko J,Bähr M,Kügler S

更新日期：2013-07-16 00:00:00

abstract：:The popularization of genome-wide analyses and RNA sequencing led to the discovery that a large part of the human genome, while effectively transcribed, does not encode proteins. Long non-coding RNAs have emerged as critical regulators of gene expression in both normal and disease states. Studies of long non-coding RN...

journal_title：Molecular therapy. Nucleic acids

authors： Gomes CPC,Spencer H,Ford KL,Michel LYM,Baker AH,Emanueli C,Balligand JL,Devaux Y,Cardiolinc network.

更新日期：2017-09-15 00:00:00

abstract：:Lipid nanoparticles (LNPs) have been used to successfully deliver small interfering RNAs (siRNAs) to target cells in both preclinical and clinical studies and currently are the leading systems for in vivo delivery. Here, we propose the use of an ordinary differential equation (ODE)-based model as a tool for optimizing...

journal_title：Molecular therapy. Nucleic acids

authors： Mihaila R,Ruhela D,Keough E,Cherkaev E,Chang S,Galinski B,Bartz R,Brown D,Howell B,Cunningham JJ

更新日期：2017-06-16 00:00:00