Abstract:
:Current acute myeloid leukemia (AML) disease models face severe limitations because most of them induce un-physiological gene expressions that do not represent conditions in AML patients and/or depend on external promoters for regulation of gene expression/repression. Furthermore, many AML models are based on reciprocal chromosomal translocations that only reflect the minority of AML patients, whereas more than 50% of patients have a normal karyotype. The majority of AML, however, is driven by somatic mutations. Thus, identification as well as a detailed molecular and functional characterization of the role of these driver mutations via improved AML models is required for better approaches toward novel targeted therapies. Using the IDH2 R140Q mutation as a model, we present a new effective methodology here using the RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system to reproduce or remove AML-associated mutations in or from human leukemic cells, respectively, via introduction of a DNA template at the endogenous gene locus via homologous recombination. Our technology represents a precise way for AML modeling to gain insights into AML development and progression and provides a basis for future therapeutic approaches.
journal_name
Mol Ther Nucleic Acidsjournal_title
Molecular therapy. Nucleic acidsauthors
Brabetz O,Alla V,Angenendt L,Schliemann C,Berdel WE,Arteaga MF,Mikesch JHdoi
10.1016/j.omtn.2016.12.012subject
Has Abstractpub_date
2017-03-17 00:00:00pages
243-248issn
2162-2531pii
S2162-2531(17)30001-Xjournal_volume
6pub_type
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journal_title:Molecular therapy. Nucleic acids
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journal_title:Molecular therapy. Nucleic acids
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journal_title:Molecular therapy. Nucleic acids
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
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doi:10.1016/j.omtn.2020.08.003
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journal_title:Molecular therapy. Nucleic acids
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doi:10.1016/j.omtn.2018.11.006
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章,评审
doi:10.1016/j.omtn.2020.05.011
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
doi:10.1038/mtna.2015.2
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
doi:10.1016/j.omtn.2017.12.008
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
doi:10.1016/j.omtn.2019.07.005
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
doi:10.1016/j.omtn.2017.07.011
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journal_title:Molecular therapy. Nucleic acids
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journal_title:Molecular therapy. Nucleic acids
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doi:10.1016/j.omtn.2017.11.010
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
doi:10.1016/j.omtn.2020.09.026
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journal_title:Molecular therapy. Nucleic acids
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doi:10.1016/j.omtn.2019.05.008
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journal_title:Molecular therapy. Nucleic acids
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journal_title:Molecular therapy. Nucleic acids
pub_type: 杂志文章
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journal_title:Molecular therapy. Nucleic acids
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