Abstract：
:Besides the acquisition of pharmacokinetic parameters of antisense oligonucleotide microRNA (miRNA) inhibitors, such as measuring in vivo concentration, their pharmacodynamic characteristics are also of interest. An emerging and straightforward method for studying molecular interactions is microscale thermophoresis (MST). This technique makes it possible to study interactions between miRNAs and various oligonucleotide inhibitors, independent of the chemical modifications of the inhibitors or their respective target structure, with very little sample volume required compared to competitive techniques, such as surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC). Interaction studies between these inhibitors and their respective target structures were performed, and they allowed the assessment of binding characteristics and parameters, such as EC50 for a number of these inhibitors, with little effort. Furthermore, MST could be utilized for obtaining kinetic binding data of the Argonaute-2 protein with a miRNA, which showed a possible RNA-induced silencing complex (RISC)-mediated turnover of inhibited miRNAs.
journal_name
Mol Ther Nucleic Acidsjournal_title
Molecular therapy. Nucleic acidsauthors
Herkt M,Batkai S,Thum Tdoi
10.1016/j.omtn.2019.08.019subject
Has Abstractpub_date
2019-12-06 00:00:00pages
259-268issn
2162-2531pii
S2162-2531(19)30234-3journal_volume
18pub_type
杂志文章abstract：:VPS33B is reported to be a tumor suppressor in hepatocellular carcinoma, nasopharyngeal carcinoma, colon cancer, and lung adenocarcinoma. Here, we observed that reduced VPS33B protein level was an unfavorable factor that promoted the pathogenesis of non-small cell lung cancer (NSCLC) in clinical specimens. We achieved...
journal_title：Molecular therapy. Nucleic acids
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更新日期：2020-11-17 00:00:00
abstract：:Cystic fibrosis (CF) is a recessive inherited disease associated with multiorgan damage that compromises epithelial and inflammatory cell function. Induced pluripotent stem cells (iPSCs) have significantly advanced the potential of developing a personalized cell-based therapy for diseases like CF by generating patient...
journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
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journal_title：Molecular therapy. Nucleic acids
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
doi：10.1038/mtna.2016.20
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journal_title：Molecular therapy. Nucleic acids
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journal_title：Molecular therapy. Nucleic acids
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journal_title：Molecular therapy. Nucleic acids
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.omtn.2018.09.020
更新日期：2018-12-07 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
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更新日期：2020-08-29 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
doi：10.1016/j.omtn.2020.01.004
更新日期：2020-03-06 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
doi：10.1016/j.omtn.2018.08.015
更新日期：2018-12-07 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
doi：10.1016/j.omtn.2019.12.007
更新日期：2020-03-06 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
doi：10.1016/j.omtn.2020.06.003
更新日期：2020-09-04 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
doi：10.1016/j.omtn.2019.10.036
更新日期：2020-03-06 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Molecular therapy. Nucleic acids
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
doi：10.1016/j.omtn.2020.12.003
更新日期：2020-12-10 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
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更新日期：2019-09-06 00:00:00
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journal_title：Molecular therapy. Nucleic acids
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更新日期：2019-03-01 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
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更新日期：2019-12-06 00:00:00
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journal_title：Molecular therapy. Nucleic acids
pub_type： 杂志文章
doi：10.1016/j.omtn.2020.10.036
更新日期：2020-10-31 00:00:00
