Abstract:
BACKGROUND:Tuberculosis (TB) is the major cause of death in Human Immunodeficiency Virus (HIV)-infected individuals. However, diagnosis of TB in HIV remains challenging particularly when HIV infection is advanced. Several gene signatures and serum protein biomarkers have been identified that distinguish active TB from latent infection. Our study was designed to assess if gene expression signatures and cytokine levels would distinguish active TB in advanced HIV. METHODS:We conducted a case-control study of whole blood RNA-Seq and plasma cytokine/chemokine analysis in HIV-infected with CD4+ T cell count of ≤ 100 cells/μl, with and without active TB. Next, the overlap of the differentially expressed genes (DEG) with the published signatures was performed and then receiver operator characteristic (ROC) analysis was done on small gene discriminators to determine their performance in distinguishing TB in advanced HIV. ELISA was performed on plasma to evaluate cytokine and chemokine levels. RESULTS:Hierarchical clustering of the transcriptional profiles showed that, in general, HIV-infected individuals with TB (TB-HIV) clustered separately from those without TB. IPA indicated that the TB-HIV signature was characterized by an increase in inflammatory signaling pathways. Analysis of overlaps between DEG in our data set with published TB signatures revealed that significant overlap was seen with one TB signature and one TB-IRIS signature. ROC analysis revealed that transcript levels of FcGR1A (AUC = 0.85) and BATF2 (AUC = 0.82), previously reported as consistent single gene classifiers of active TB irrespective of HIV status, performed successfully even in advanced HIV. Plasma protein levels of IFNγ, a stimulator of FcGR1A and BATF2, and CXCL10, also up-regulated by IFNγ, accurately classified active TB (AUC = 0.98 and 0.91, respectively) in advanced HIV. Neither of these genes nor proteins distinguished between TB and TB-IRIS. CONCLUSIONS:Gene expression of FcGR1A and BATF2, and plasma protein levels of IFNγ and CXCL10 have the potential to independently detect TB in advanced HIV. However, since other lung diseases were not included in this study, these final candidates need to be validated as specific to TB in the advanced HIV population with TB.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Verma S,Du P,Nakanjako D,Hermans S,Briggs J,Nakiyingi L,Ellner JJ,Manabe YC,Salgame Pdoi
10.1186/s12879-018-3127-4subject
Has Abstractpub_date
2018-05-15 00:00:00pages
220issue
1issn
1471-2334pii
10.1186/s12879-018-3127-4journal_volume
18pub_type
杂志文章abstract:BACKGROUND:A more stringent QuantiFERON-TB Gold In-Tube (QFT) conversion (from negative to positive) definition has been proposed to allow more definite detection of recent tuberculosis (TB) infection. We explored alternative conversion definitions to assist the interpretation of serial QFT results and estimate inciden...
journal_title:BMC infectious diseases
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12879-020-05483-9
更新日期:2020-10-15 00:00:00
abstract:BACKGROUND:Pregnant women in malaria endemic areas are at high risk of P. falciparum infection and its complications. This study investigated the prevalence and risk factors for P. falciparum infection and malaria among pregnant women reporting for first antenatal care (ANC) clinic visit in the mount Cameroon area. ME...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1211-6
更新日期:2015-10-22 00:00:00
abstract:BACKGROUND:Lamivudine inhibits replication of both human immunodeficiency virus (HIV) and hepatitis B virus (HBV) and is commonly used as part of antiretroviral therapy. The main limitation in the use of lamivudine is resistant mutation selection. Most of these mutations affect the YMDD motif of the HBV DNA polymerase....
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-4-29
更新日期:2004-08-31 00:00:00
abstract:BACKGROUND:Dengue has global importance as a dreaded arboviral infection. It has 4 serotypes of epidemiological imporatnce. The classification denotes two clinical spectrums- dengue fever (DF) and dengue haemorragic fever (DHF). Most cases are stereotype and amenable to fluid resuscitation. However, unusual manifestati...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3596-5
更新日期:2018-12-18 00:00:00
abstract:BACKGROUND:This study aims to research two areas, one with a resistant and the other with a susceptible profile of An. gambiae to deltamethrin in the region of Plateau (southern Benin). In each area, eight localities were sought. Both areas were needed for the assessment of the impact of malaria vector resistance to py...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-103
更新日期:2014-02-25 00:00:00
abstract:BACKGROUND:Early infant diagnosis of HIV infection is challenging in sub-Saharan Africa, particularly in rural areas, leading to delays in diagnosis and treatment. Use of a point-of-care test would overcome many challenges. This study evaluated the validity of a novel point-of-care p24 antigen detection test (LYNX) in ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-021-05808-2
更新日期:2021-01-26 00:00:00
abstract:BACKGROUND:Following delivery by caesarean section, surgical site infection is the most common infectious complication. Despite a large number of caesarean sections performed at Debre Markos Referral Hospital, there was no study documenting the incidence of surgical site infection after caesarean section. Therefore, th...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05640-0
更新日期:2020-11-30 00:00:00
abstract:BACKGROUND:Q fever is caused by the intracellular bacterium Coxiella burnetii. Initial infection can present as acute Q fever, while a minority of infected individuals develops chronic Q fever endocarditis or vascular infection months to years after initial infection. Serology is an important diagnostic tool for both a...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-330
更新日期:2014-06-15 00:00:00
abstract:BACKGROUND:Dengue is endemic in over 100 countries and is an important public health problem worldwide. Dengue fever is not endemic in Taiwan; the importation of dengue viruses from neighboring countries via close commercial links and air travel is considered to be the cause of local outbreaks. Therefore, efforts towar...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-4942-y
更新日期:2020-05-15 00:00:00
abstract:BACKGROUND:The rise in antibiotic resistance is a global public health concern, and antibiotic overuse needs to be reduced. Earlier studies of out-of-hours care have indicated that antibiotic prescribing is less appropriate than that of in-hours care. However, no study has compared the out-of-hours treatment of infecti...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05334-7
更新日期:2020-08-20 00:00:00
abstract:BACKGROUND:In developing countries rotavirus is the leading cause of severe diarrhoea and diarrhoeal deaths in children under 5. Vaccination could greatly alleviate that burden, but in Mexico as in most low- and middle-income countries the decision to add rotavirus vaccine to the national immunisation program will depe...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-8-103
更新日期:2008-07-29 00:00:00
abstract:BACKGROUND:This study aims to investigate the clinical features of invasive community-acquired Staphylococcus aureus (CA-SA) infection in Chinese children and analyze its molecular features. METHODS:Clinical data and invasive CA-SA isolates were prospectively collected. Pediatric risk of mortality (PRISM) score was us...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-014-0582-4
更新日期:2014-11-07 00:00:00
abstract:BACKGROUND:The association of Chlamydia pneumoniae with atherosclerosis is controversial. We investigated the presence of C. pneumoniae and other Chlamydia spp. in atheromatous carotid artery tissue. METHODS:Forty elective carotid endarterectomy patients were recruited (27 males, mean age 65 and 13 females mean age 68...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-1-12
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:The study of the endotoxin tolerance phenomenon in light of the recently defined roles of mast cells and toll-like receptors as essential components of the innate immune response and as orchestrators of acquired immunity may reveal potentially useful mechanisms of immunomodulation of infectious and allergic ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-10-240
更新日期:2010-08-14 00:00:00
abstract:BACKGROUND:Salmonella enterica, serovar Typhi (S. Typhi), a causative agent of enteric fever (typhoid fever), predominately affects populations in developing regions with poor access to clean food and water. In addition, travelers to these regions are at risk of exposure. METHODS:We report the epidemiological characte...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1159-6
更新日期:2015-10-14 00:00:00
abstract:BACKGROUND:Despite the availability of antiretroviral therapy (ART), suboptimal treatment outcomes have been observed among HIV-seropositive illicit drug users. As there is an urgent need to improve responses to antiretroviral therapy among this population, we undertook this study to evaluate the role of physician expe...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-22
更新日期:2012-01-25 00:00:00
abstract:BACKGROUND:COVID-19 is novel infectious disease with an evolving understanding of its epidemiology and clinical manifestations. Severe cases developed life-threatening complications, such as respiratory failure, shock, and multiple organs dysfunction. Immunocompromised patients often present atypical presentations of v...
journal_title:BMC infectious diseases
pub_type: 杂志文章,评审
doi:10.1186/s12879-020-05480-y
更新日期:2020-10-19 00:00:00
abstract:BACKGROUND:Human papillomavirus (HPV) associated malignancies are the leading cause of cancer death in Botswana. We sought to determine causative HPV types in patients with anogenital malignancies in Botswana to inform vaccine strategy. METHODS:We used formalin-fixed and paraffin-embedded (FFPE) tissue blocks from pat...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2832-8
更新日期:2017-11-25 00:00:00
abstract:BACKGROUND:To verify whether a daily service of Infectious Diseases consultation (ID-cons) is more effective than a weekly service in reducing antibiotic (ATB) consumption without worsening of clinical outcomes. METHODS:Two-year observational analysis of the ID-cons provided in a hospital setting in Milan, Italy. ID-c...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05550-1
更新日期:2020-11-07 00:00:00
abstract:BACKGROUND:Chronic rhinosinusitis is an inflammatory disorder in which the role of bacteria remains uncertain. While sinus outflow obstruction is often an initiating event, mucosal inflammation and dysbiosis may persist or develop in sinuses with widely patent surgical openings. Understanding of the relationship betwee...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3272-9
更新日期:2018-08-30 00:00:00
abstract:BACKGROUND:Dengue virus (DENV) infection is an emerging arboviral infection in tropical and sub-tropical countries in South-East Asia, the Western Pacific and South and Central America. Secondary DENV infection is the most widely accepted risk factor for the development of severe forms. Methods to discriminate primary ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1022-9
更新日期:2015-07-21 00:00:00
abstract:BACKGROUND:Tuberculous meningitis (TBM) represents a diagnostic and management challenge to clinicians. The "Thwaites' system" and "Lancet consensus scoring system" are utilized to differentiate TBM from bacterial meningitis but their utility in subacute and chronic meningitis where TBM is an important consideration is...
journal_title:BMC infectious diseases
pub_type: 杂志文章,多中心研究
doi:10.1186/s12879-020-05502-9
更新日期:2020-10-23 00:00:00
abstract:BACKGROUND:Retaining patients on antiretroviral treatment in care is critical to sustaining the 90:90:90 vision. Nigeria has made some progress in placing HIV-positive patients on treatment. In an effort to increase access to treatment, ART decentralization has been implemented in the country. This is aimed at strength...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4293-8
更新日期:2019-07-22 00:00:00
abstract:BACKGROUND:Urinary tract infection is an infection affecting infants and children. The aim of this study was to determine the etiology of urinary tract infection along with their antimicrobial resistance. METHODS:This cross-sectional study was conducted from June 2015 to January 2016 at Siddhi Memorial Hospital, Bhakt...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3997-0
更新日期:2019-05-14 00:00:00
abstract:BACKGROUND:Vitamin D enhances host protective immune responses to Mycobacterium tuberculosis by suppressing Interferon-gamma (IFN-g) and reducing disease associated inflammation in the host. The objectives of this study were to determine whether vitamin D supplementation to patients with tuberculosis (TB) could influen...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1186/1471-2334-13-22
更新日期:2013-01-19 00:00:00
abstract:BACKGROUND:Pneumocystis jiroveci (formerly known as P. carinii f.sp. hominis) is an opportunistic fungus that causes Pneumocystis pneumonia (PCP) in immunocompromised individuals. Pneumocystis jiroveci can be detected by polymerase chain reaction (PCR). To investigate the clinical importance of a positive Pneumocystis-...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-2-28
更新日期:2002-11-25 00:00:00
abstract:BACKGROUND:Leishmaniasis caused by two new species of Leishmania; L. siamensis and L. martiniquensis have been recently described in Thailand. The disease has mainly been documented in AIDS patients from southern Thailand. In this study, polymerase chain reaction (PCR) was used to determine HIV-Leishmania co-infection ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1433-2
更新日期:2016-02-24 00:00:00
abstract:BACKGROUND:Toxoplasmosis is a worldwide zoonosis caused by the intracellular parasite Toxoplasma gondii. However, no effective vaccine is yet available. Poly(lactide-co-glycolide) polymers can reduce protein degradation and sustain the release of antigens over a long period, which could generate a long-lasting immune r...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-1496-0
更新日期:2016-04-18 00:00:00
abstract:BACKGROUND:Post-exposure prophylaxis (PEP) following sexual exposure to HIV has been recommended as a method of preventing HIV infection in the UK. Men who have sex with men (MSM) are the group most affected by HIV in the UK and their sexual risk taking behaviour is reported to be increasing. One-to-one behavioural int...
journal_title:BMC infectious diseases
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/1471-2334-12-70
更新日期:2012-03-22 00:00:00
abstract:BACKGROUND:The Middle East respiratory syndrome (MERS) is proposed to be a zoonotic disease. Dromedary camels have been implicated due to reports that some confirmed cases were exposed to camels. Risk factors for MERS coronavirus (MERS-CoV) infections in humans are incompletely understood. This study aimed to describe ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-2137-3
更新日期:2017-01-05 00:00:00