Abstract：
BACKGROUND:Leishmaniasis caused by two new species of Leishmania; L. siamensis and L. martiniquensis have been recently described in Thailand. The disease has mainly been documented in AIDS patients from southern Thailand. In this study, polymerase chain reaction (PCR) was used to determine HIV-Leishmania co-infection in southern Thailand. METHODS:One ml of saliva and 3 ml of EDTA blood were collected from HIV-infected patients for PCR detection of Leishmania DNA, cloning and sequencing. The positive PCR samples were then cultured on Schneider's insect medium. RESULTS:Three out of 316 saliva samples collected from HIV-infected patients were found to be positive for Leishmania DNA (0.95%). Among the positive samples, one patient was observed with disseminated cutaneous lesions and also tested positive via saliva, whole blood and buffy coat in PCR. The second case presenting with nodular lesions also gave a positive saliva test via PCR two months prior to buffy coat. This diagnosis was confirmed by microscopic examination and a culture of biopsy samples from a nodule. The last case was an asymptomatic Leishmania infection which tested PCR positive only in saliva with a consecutive sample collection conducted for three months. CONCLUSIONS:The prevalence of Leishmania infection in HIV infected patients within this study is 0.95%. Leishmania DNA was detected in saliva by PCR prior to blood and buffy coat of two HIV infected patients. Early detection of Leishmania DNA in saliva would be beneficial for the follow up of asymptomatic Leishmania infected patients, the early treatment of leishmaniasis and for surveillance survey purpose. However, full evaluation of sensitivity and specificity of this technique with a large cohort of patients is required before deployment.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Siriyasatien P,Chusri S,Kraivichian K,Jariyapan N,Hortiwakul T,Silpapojakul K,Pym AM,Phumee Adoi
10.1186/s12879-016-1433-2subject
Has Abstractpub_date
2016-02-24 00:00:00pages
89issn
1471-2334pii
10.1186/s12879-016-1433-2journal_volume
16pub_type
杂志文章abstract：BACKGROUND:In developing countries rotavirus is the leading cause of severe diarrhoea and diarrhoeal deaths in children under 5. Vaccination could greatly alleviate that burden, but in Mexico as in most low- and middle-income countries the decision to add rotavirus vaccine to the national immunisation program will depe...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/1471-2334-8-103
更新日期：2008-07-29 00:00:00
abstract：BACKGROUND:The study of the endotoxin tolerance phenomenon in light of the recently defined roles of mast cells and toll-like receptors as essential components of the innate immune response and as orchestrators of acquired immunity may reveal potentially useful mechanisms of immunomodulation of infectious and allergic ...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/1471-2334-10-240
更新日期：2010-08-14 00:00:00
abstract：BACKGROUND:Hepatitis B virus (HBV) DNA levels are crucial for managing chronic hepatitis B (CHB). It was unclear whether Daan real-time polymerase chain reaction test (Daan test) or COBAS TaqMan HBV DNA Test (Cobas TaqMan) was superior in measuring different HBV DNA levels in clinical specimens. METHODS:We enrolled 67...
journal_title：BMC infectious diseases
pub_type： 杂志文章,多中心研究
doi：10.1186/1471-2334-14-85
更新日期：2014-02-14 00:00:00
abstract：BACKGROUND:In Colombia, the dengue virus (DENV) has been endemic for decades, and with the recent entry of the chikungunya virus (CHIKV) (2014) and the Zika virus (ZIKV) (2015), health systems are overloaded because the diagnosis of these three diseases is based on clinical symptoms, and the three diseases share a symp...
journal_title：BMC infectious diseases
pub_type： 杂志文章
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abstract：BACKGROUND:Immunization policymakers at global and local levels need to establish priorities among new vaccines competing for limited resources. However, comparison of the potential impact of single vaccination programs is challenging, primarily due to the limited number of vaccine analyses as well as their differing a...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/1471-2334-11-174
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abstract：BACKGROUND:Collecting adequate information on key epidemiological indicators is a prerequisite to informing a public health response to reduce the impact of hepatitis C virus (HCV) infection in Malaysia. Our goal was to overcome the acute data shortage typical of low/middle income countries using statistical modelling ...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-014-0564-6
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abstract：BACKGROUND:Reactivation of hepatitis B virus (HBV), characterized by increased levels of serum HBV DNA, abnormal liver function and hepatic failure, is a frequent complication of immunosuppressive therapy and chemotherapy in patients with HBV infection. However, reactivation of occult HBV infection with immunosuppressi...
journal_title：BMC infectious diseases
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abstract：BACKGROUND:The emergence of extensively drug-resistant tuberculosis (XDR-TB) has raised public health concern for global TB control. Although multi drug-resistant tuberculosis (MDR- TB) prevalence and associated genetic mutations in Morocco are well documented, scarce information on XDR TB is available. Hence, the eval...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-018-3009-9
更新日期：2018-02-27 00:00:00
abstract：BACKGROUND:Group B streptococcus (GBS) is reported as the leading cause of neonatal sepsis and meningitis. Newborns from GBS colonized pregnant women are at high risk of infection. METHOD:A Hospital based cross-sectional study was conducted at Hawassa University Comprehensive Specialized Hospital from November 05, 201...
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abstract：BACKGROUND:ProvLab Alberta provides all laboratory testing for Bordetella pertussis including sporadic cases and outbreak investigations through collaborations with provincial public health partners. We describe B. pertussis activity in Alberta from July 2004 to December 2012. METHODS:Laboratory testing for pertussis ...
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journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-016-1678-9
更新日期：2016-07-22 00:00:00
abstract：BACKGROUND:Since correct maintenance of intravascular catheters is an effective strategy for preventing central-line infections, the aim of this study was to assess the level of adherence to guidelines for CVC maintenance amongst Italian HCWs. METHODS:From July 2016 thru January 2017, a cross sectional survey was carr...
journal_title：BMC infectious diseases
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更新日期：2018-12-03 00:00:00
abstract：BACKGROUND:No comparison data have been reported on viral and epidemiological profiles of hospitalized children with severe acute respiratory infection (SARI) in Beijing or Shanghai, China. METHODS:We collected 700 nasopharyngeal aspirates (NPA) from hospitalized children with SARI in Beijing (northern China) and Shan...
journal_title：BMC infectious diseases
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abstract：BACKGROUND:The diagnostic accuracy of fine-needle aspiration (FNA) cytology for the diagnosis of tuberculous lymphadenitis (TBLN) is confounded by mimicking cytomorphologic disorders. The objective of this study was to determine whether supplementing FNA cytology with bacteriological methods improves the overall accura...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-014-0720-z
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abstract：BACKGROUND:Some children are prone to recurrent invasive pneumococcal disease (rIPD) and of these, some respond insufficiently to standard pneumococcal vaccination. Little is known about how to handle these children and if they benefit from additional vaccination. Here, we present results from a nationwide study of ped...
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doi：10.1186/s12879-018-3267-6
更新日期：2018-08-06 00:00:00
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doi：10.1186/1471-2334-9-45
更新日期：2009-04-20 00:00:00
abstract：BACKGROUND:Screening and treatment of latent tuberculosis infection (LTBI) in asylum seekers (AS) may prevent future cases of tuberculosis. As the screening with Interferon Gamma Release Assay (IGRA) is costly, the objective of this study was to assess which factors were associated with LTBI and to define a score allow...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/1471-2334-12-285
更新日期：2012-11-02 00:00:00
abstract：BACKGROUND:The swine influenza H1N1 first identified in Mexico, spread rapidly across the globe and is considered the fastest moving pandemic in history. The early phase of an outbreak, in which data is relatively scarce, presents scientific challenges on key issues such as: scale, severity and immunity which are funda...
journal_title：BMC infectious diseases
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doi：10.1186/1471-2334-11-92
更新日期：2011-04-14 00:00:00
abstract：BACKGROUND:Substantial numbers of patients are now receiving either immunosuppressive therapies or chemotherapy. There are significant risks in such patients of developing opportunistic infections or re-activation of latent infections, with higher associated morbidity and mortality. The aim of this quality improvement ...
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journal_title：BMC infectious diseases
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abstract：BACKGROUND:Eight genotypes of Hepatitis B virus designated A-H, have been known but in Pakistan, no such data is available on the prevalent HBV genotypes. Therefore, the subject study was conducted to determine HBV genotypes in the indigenous Pakistani population. METHODS:A total of 690 individuals were enrolled for H...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/1471-2334-7-115
更新日期：2007-10-08 00:00:00
abstract：BACKGROUND:Despite Noroviruses (NV, previously "Norwalk-like viruses") being a leading cause of acute gastroenteritis outbreaks, the impact of NV infection is at present unknown and little information is available about strains circulating in Italy. In April 2002 an outbreak of gastroenteritis occurred in the province ...
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pub_type： 杂志文章
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更新日期：2004-09-21 00:00:00
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journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/1471-2334-12-6
更新日期：2012-01-14 00:00:00
abstract：BACKGROUND:Although Indonesia has high fatality rate of human A/H5N1 cases, epidemiological and clinical data on influenza virus circulation among humans has been limited. Within Indonesia, Bali province is of interest due to high population densities of humans, pigs and poultry. This study aims to characterize and com...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-019-3842-5
更新日期：2019-03-07 00:00:00
abstract：BACKGROUND:Carbapenemase-producing organisms (CPOs) have emerged as antibiotic-resistant bacteria of global concern. Here we assessed the performance of the Carba (beta) assay, a multiplex real-time PCR assay developed by SpeeDx for the detection of key carbapenemase-encoding genes: KPC, NDM, OXA-48-like, IMP-4-like, a...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-019-4176-z
更新日期：2019-07-02 00:00:00
abstract：BACKGROUND:The aim of the study was to assess the correlation between the tuberculin skin test (TST) and in vitro interferon-gamma released assays (IGRAs) with risk factors for the spread of infection in smear positive pulmonary tuberculosis (TB) contacts. METHODS:We recruited prospective contacts with smear positive ...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/1471-2334-14-258
更新日期：2014-05-13 00:00:00
abstract：BACKGROUND:Over 80% of morbidity due to soil-transmitted helminthiasis (STH) occurs in low-income countries. Children under 5 account for 20-30% of the burden in endemic areas. This study assessed the prevalence, intensity and factors associated with STH infections among preschool-age children (PSAC) in Hoima district,...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-018-3289-0
更新日期：2018-08-17 00:00:00
abstract：BACKGROUND:The outbreak of the 2009 H1N1 influenza pandemic (H1N1pdm) affected thousands of people in Mexico and the United States, and spread rapidly throughout the world from April 2009 to July 2010. To explore the age-specific prevalence of seroprotection against H1N1pdm infection, we estimated pre-existing humoral ...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-017-2215-1
更新日期：2017-02-10 00:00:00
abstract：BACKGROUND:Human papillomavirus (HPV) associated malignancies are the leading cause of cancer death in Botswana. We sought to determine causative HPV types in patients with anogenital malignancies in Botswana to inform vaccine strategy. METHODS:We used formalin-fixed and paraffin-embedded (FFPE) tissue blocks from pat...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-017-2832-8
更新日期：2017-11-25 00:00:00
abstract：BACKGROUND:Adjustment of immunosuppression is the main therapy for BK polyomavirus (BKPyV)-associated nephropathy (BKPyVAN) after kidney transplantation (KT). Studies of BKPyV-specific T cell immune response are scarce. Here, we investigated BKPyV-specific T cell immunity in KT recipients diagnosed with BKPyVAN. METHO...
journal_title：BMC infectious diseases
pub_type： 杂志文章
doi：10.1186/s12879-019-4615-x
更新日期：2019-11-19 00:00:00
