Abstract:
BACKGROUND:Lung cancer is one of the leading causes of cancer related deaths worldwide. Marine microalgae are a source of biologically active compounds and are widely consumed as a nutritional supplement in East Asian countries. It has been reported that Chlorella or Chlorella extracts have various beneficial pharmacological compounds that modulate immune responses; however, no studies have investigated the anti-cancer effects of Chlorella sorokiniana (CS) on non-small cell lung cancer (NSCLC). METHODS:In this study, we evaluated the anti-cancer effects of CS in two human NSCLC cell lines (A549 and CL1-5 human lung adenocarcinoma cells), and its effects on tumor growth in a subcutaneous xenograft tumor model. We also investigated the possible molecular mechanisms governing the pharmacological function of CS. RESULTS:Our results showed that exposure of the two cell lines to CS resulted in a concentration-dependent reduction in cell viability. In addition, the percentage of apoptotic cells increased in a dose-dependent manner, suggesting that CS might induce apoptosis in human NSCLC cells. Western blot analysis revealed that exposure to CS resulted in increased protein expression of the cleaved/activated forms of caspase-3, caspase-9, and PARP, except caspase-8. ZDEVD (caspase-3 inhibitor) and Z-LEHD (caspase-9 inhibitor) were sufficient at preventing apoptosis in both A549 and CL1-5 cells, proving that CS induced cell death via the mitochondria-mediated apoptotic pathway. Exposure of A549 and CL1-5 cells to CS for 24 h resulted in decreased expression of Bcl-2 protein and increased expression of Bax protein as well as decreased expression of two IAP family proteins, survivin and XIAP. CONCLUSIONS:We demonstrated that CS induces mitochondrial-mediated apoptosis in NSCLC cells via downregulation of Bcl-2, XIAP and survivin. In addition, we also found that the tumors growth of subcutaneous xenograft in vivo was markedly inhibited after oral intake of CS.
journal_name
BMC Complement Altern Medjournal_title
BMC complementary and alternative medicineauthors
Lin PY,Tsai CT,Chuang WL,Chao YH,Pan IH,Chen YK,Lin CC,Wang BYdoi
10.1186/s12906-017-1611-9subject
Has Abstractpub_date
2017-02-01 00:00:00pages
88issue
1issn
1472-6882pii
10.1186/s12906-017-1611-9journal_volume
17pub_type
杂志文章abstract:BACKGROUND:Chinese herbal formulae are composed of complex components and produce comprehensive pharmacological effects. Unlike chemical drugs that have only one clear single target, the components of Chinese herbal formulae have multiple channels and targets. How to discover the pharmacological targets of Chinese herb...
journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,随机对照试验
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,多中心研究,随机对照试验
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,随机对照试验
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
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更新日期:2019-11-12 00:00:00
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journal_title:BMC complementary and alternative medicine
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更新日期:2017-05-03 00:00:00
abstract:BACKGROUND:Medical students have consistently expressed interest in learning about alternative healing modalities, especially herbal and natural products. To fill this void in medical education at our institution, a novel elective was developed and implemented for fourth year medical students. This herbal/natural produ...
journal_title:BMC complementary and alternative medicine
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doi:10.1186/1472-6882-12-57
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-017-1992-9
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
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更新日期:2015-03-19 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-015-0827-9
更新日期:2015-08-29 00:00:00
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journal_title:BMC complementary and alternative medicine
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更新日期:2017-09-04 00:00:00