Abstract:
BACKGROUND:The aim of this research was to determine the intensity and mechanisms of the cytotoxic actions of five extracts isolated from the endemic plant species Helichrysum zivojinii Černjavski & Soška (family Asteraceae) against specific cancer cell lines. In order to evaluate the sensitivity of normal immunocompetent cells implicated in the antitumor immune response, the cytotoxicity of extracts was also tested against healthy peripheral blood mononuclear cells (PBMC). METHODS:The aerial parts of the plants were air-dried, powdered, and successively extracted with solvents of increasing polarity to obtain hexane, dichloromethane, ethyl-acetate, n-butanol and methanol extracts. The cytotoxic activities of the extracts against human cervix adenocarcinoma HeLa, human melanoma Fem-x, human myelogenous leukemia K562, human breast adenocarcinoma MDA-MB-361 cells and PBMC were evaluated by the MTT test. The mode of HeLa cell death was investigated by morphological analysis. Changes in the cell cycle of HeLa cells treated with the extracts were analyzed by flow cytometry. The apoptotic mechanisms induced by the tested extracts were determined using specific caspase inhibitors. RESULTS:The investigated Helichrysum zivojinii extracts exerted selective dose-dependent cytotoxic actions against selected cancer cell lines and healthy immunocompetent PBMC stimulated to proliferate, while the cytotoxic actions exerted on unstimulated PBMC were less pronounced. The tested extracts exhibited considerably stronger cytotoxic activities towards HeLa, Fem-x and K562 cells in comparison to resting and stimulated PBMC. It is worth noting that the cytotoxicity of the extracts was weaker against unstimulated PBMC in comparison to stimulated PBMC. Furthermore, each of the five extracts induced apoptosis in HeLa cells, through the activation of both intrinsic and extrinsic signaling pathways. CONCLUSION:Extracts obtained from the endemic plant Helichrysum zivojinii may represent an important source of novel potential antitumor agents due to their pronounced and selective cytotoxic actions towards malignant cells.
journal_name
BMC Complement Altern Medjournal_title
BMC complementary and alternative medicineauthors
Matić IZ,Aljančić I,Žižak Ž,Vajs V,Jadranin M,Milosavljević S,Juranić ZDdoi
10.1186/1472-6882-13-36subject
Has Abstractpub_date
2013-02-18 00:00:00pages
36issn
1472-6882pii
1472-6882-13-36journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Berberis vulgaris is a well known plant with traditional herbal medical history. The aims of this study was to bioscreen and compare the in vitro biological activity (antioxidant, cholinergic, antidaibetic and the anticancer) of barberry crude extract and berberine active compound. METHODS:The effect of B. ...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-218
更新日期:2013-09-05 00:00:00
abstract:BACKGROUND:In Pakistan Fagonia olivieri (Zygophyllaceae) is commonly used in the indigenous system of medicine for treatment of conditions like diabetes, cancer, fever, asthma, toothache, stomach troubles and kidney disorders. This study evaluated the crude methanol extract of F. olivieri (FOM) and its derived fraction...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-167
更新日期:2013-07-10 00:00:00
abstract:BACKGROUND:Main concerns for lactating women about medications include the safety of their breastfed infants and the potential effects of medication on quantity and quality of breast milk. While medicine treatments include conventional and complementary medicines, most studies to date have focused on evaluating the saf...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-317
更新日期:2013-11-13 00:00:00
abstract:BACKGROUND:Primary insomnia is a widespread and refractory disease. Moxibustion therapy for insomnia shows some advantages compared with conventional therapies. This systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted to evaluate the effectiveness and safety of moxibustion therapy f...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s12906-016-1179-9
更新日期:2016-07-13 00:00:00
abstract:BACKGROUND:Microbial species in the brine pools of the Red Sea and the brine pool-seawater interfaces are exposed to high temperature, high salinity, low oxygen levels and high concentrations of heavy metals. As adaptations to these harsh conditions require a large suite of secondary metabolites, these microbes have a ...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-019-2554-0
更新日期:2019-06-20 00:00:00
abstract:BACKGROUND:The National Institutes of Health reported in 2007 that approximately 38% of United States adults have used at least one type of Complementary and Alternative Medicine (CAM). There are no studies available that assess general CAM use in US pregnant women.The objectives of our study were to determine the prev...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-14-85
更新日期:2014-03-04 00:00:00
abstract:BACKGROUND:Previous animal studies have shown that Curcuma longa (turmeric) improves liver function. Turmeric may thus be a promising ingredient in functional foods aimed at improving liver function. The purpose of the study is to investigate the hepatoprotective effect of fermented turmeric powder (FTP) on liver funct...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,随机对照试验
doi:10.1186/1472-6882-13-58
更新日期:2013-03-08 00:00:00
abstract:BACKGROUND:To evaluate and compare 6-month effects of 8 weeks of an integrative treatment (IT), therapeutic acupuncture (TA), and conventional treatment (CT) in reducing symptoms of anxiety, depression and in improving health-related quality of life (HRQL) and sense of coherence (SOC) in psychologically distressed prim...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/1472-6882-14-210
更新日期:2014-06-30 00:00:00
abstract:BACKGROUND:The purpose of this study was to compare the efficacy of electroacupuncture (EA), diclofenac and their combination in symptomatic treatment of osteoarthritis (OA) of the knee. METHODS:This study was a randomized, single-blind, placebo controlled trial. The 193 out-patients with OA of the knee were randomize...
journal_title:BMC complementary and alternative medicine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1186/1472-6882-2-3
更新日期:2002-03-21 00:00:00
abstract:BACKGROUND:Hyaluronidases have been found as the target enzymes in the development of osteoarthritis (OA) disease. While there is still no curative treatment for this disease, recent studies on the treatment of OA were focused on the effectiveness of natural products which are expected to improve the symptoms with mini...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-213
更新日期:2013-08-23 00:00:00
abstract:BACKGROUND:Type 2 diabetes is a major health problem in many countries including India. Yoga may be an effective type 2 diabetes prevention strategy in India, particularly given its cultural familiarity. METHODS:This was a parallel, randomized controlled pilot study to collect feasibility and preliminary efficacy data...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,随机对照试验
doi:10.1186/1472-6882-14-212
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:Brown adipocytes are known to promote energy expenditure and limit weight gain to combat obesity. Averrhoa bilimbi, locally called belimbing buluh (DBB), is mainly used as an ethnomedicine in the treatment of metabolic disorders including diabetes mellitus, hypertension and obesity. The present study aims to...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-019-2640-3
更新日期:2019-09-05 00:00:00
abstract:BACKGROUND:The traditional use of the oleo-gum-resin of Commiphora guidottii Chiov. ex. Guid., which is commonly called scented myrrh, for topical treatment of wound is well documented. The major objective of the present study was to investigate the essential oil and resin obtained from C. guidottii for their potential...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-015-0813-2
更新日期:2015-08-18 00:00:00
abstract:BACKGROUND:Primary health care professionals (PHPs) usually report high levels of distress and burnout symptoms related to job strain. Mindfulness, defined as non-judgmental-present-moment awareness, seems to be a moderator in the causal association between life stressors and well-being. This study aimed to verify corr...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-015-0823-0
更新日期:2015-09-02 00:00:00
abstract:BACKGROUND:Rhabdomyolysis is an uncommon side effect of trabectedin which is used for the second line therapy of metastatic sarcoma after anthracycline and ifosfamide failure. This side effect may be due to pharmacokinetic interactions caused by shared mechanisms of metabolism involving the cytochrome P450 (CYP) system...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-199
更新日期:2013-07-30 00:00:00
abstract:BACKGROUND:National policy on medical pluralism in India encourages the mainstreaming of AYUSH (Ayurveda, Yoga, Unani, Siddha, and Homeopathy) systems and the revitalization of local health traditions (LHT). In Meghalaya state in the northeast, the main LHT is its indigenous tribal traditional medicine. This paper pres...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-015-0818-x
更新日期:2015-08-18 00:00:00
abstract:BACKGROUND:Flavonoid-rich aqueous fraction of methanolic extract of Hibiscus sabdariffa calyx was evaluated for its anti-hepatotoxic activities in streptozotocin-induced diabetic Wistar rats. METHODS:Diabetes Mellitus was induced in Wistar rats by a single i.p injection of 80 mg/kg b.w. streptozotocin (STZ) dissolved ...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-14-277
更新日期:2014-07-30 00:00:00
abstract:BACKGROUND:Increase oxidative trauma is the main cause behind Cisplatin (CP) induced cardiotoxicity which restricts its clinical application as anti-neoplastic prescription. Acacia hydaspica is a natural shrub with diverse bioactivities. Acacia hydaspica ethyl acetate extract (AHE) ameliorated drug-induced cardiotoxici...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-019-2575-8
更新日期:2019-07-23 00:00:00
abstract:BACKGROUND:Protein kinase C regulates various cellular processes including cell proliferation, cell adhesion, apoptosis, angiogenesis, invasion, and metastasis. Activation of different PKC isozymes results in distinct cellular responses. Novel PKCs are mainly involved in apoptotic process. Atypical PKC subfamily plays ...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-015-0810-5
更新日期:2015-08-15 00:00:00
abstract:BACKGROUND:Fumaria parviflora Lam. (Fumaraceae) is widely used in traditional as well as folkloric system of medicine from ancient. It is commonly known as 'Pitpapra' or 'Shahtrah' in Indian traditional system of medicine and used for treating numerous ailments like diarrhea, fever, influenza, blood purifier and other ...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-14-98
更新日期:2014-03-12 00:00:00
abstract:BACKGROUND:Our preliminary studies indicated that electroacupuncture (EA) at the ST36 and Ashi acupoints could promote regeneration of the rabbit gastrocnemius (GM) by improving microcirculation perfusion, promoting the recovery of myofiber structures, and inhibiting excessive fibrosis. However, the effects of EA on re...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-015-0601-z
更新日期:2015-03-19 00:00:00
abstract:BACKGROUND:The use of complementary and alternative medicine (CAM) continues to increase in Taiwan. This study examined the use of CAM and beliefs about CAM as expressed by patients with traumatic brain injury (TBI) in Taiwan. METHODS:TBI patients and their accompanying relatives were interviewed by using a structured...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-12-211
更新日期:2012-11-06 00:00:00
abstract:BACKGROUND:Infertility is a common complication in diabetic men and experimental animals, mainly due to loss of germ cells by apoptotic cell death. The aim of this study was to evaluate the effects of aqueous and ethanol extracts of Dracaena arborea in streptozotocin-induced ultra-structural spermatogenic alterations i...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-71
更新日期:2013-04-02 00:00:00
abstract:BACKGROUND:Breast cancer is the most common cancer in women and affects 1.38 million women worldwide per year. Antiestrogens such as tamoxifen, a selective estrogen receptor (ER) modulator, are widely used in clinics to treat ER-positive breast tumors. However, remissions of breast cancer are often followed by resistan...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-018-2204-y
更新日期:2018-05-09 00:00:00
abstract:BACKGROUND:Recently, considerable attention has been focused on exploring the potential antioxidant properties of plant extracts or isolated products of plant origin. Prunella vulgaris var. lilacina is widely distributed in Korea, Japan, China, and Europe, and it continues to be used to treat inflammation, eye pain, he...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-310
更新日期:2013-11-09 00:00:00
abstract:BACKGROUND:Candida species have been associated with the emergence of resistant strains towards selected antifungal agents. Plant products have been used traditionally as alternative medicine to ease candidal infections. The present study was undertaken to investigate the antifungal susceptibility patterns and growth i...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-342
更新日期:2013-12-04 00:00:00
abstract:BACKGROUND:Fructus Psoraleae is the seed of Psoralea corylifolia Linn. Fructus Psoraleae has been shown to be effective in treating some skin diseases, such as vitiligo. As a main ingredient in five types of herbs in the Qubaibabuqi tablet formula, Fructus Psoraleae plays an important role in the treatment of vitiligo....
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,评审
doi:10.1186/s12906-019-2493-9
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Drug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. Ophiop...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-12-238
更新日期:2012-11-28 00:00:00
abstract:BACKGROUND:Ginsenoside Rg1 (Rg1) is one of the most active ingredients in Panax ginseng and has been proven to have anti-oxidative and anti-aging properties. However, there have been few reports concerning the anti-aging effects of Rg1 on the hematopoietic microenvironment and bone marrow stromal cells (BMSCs). METHOD...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-015-0642-3
更新日期:2015-04-15 00:00:00
abstract:BACKGROUND:The imbalance between the generation of free radicals and natural cellular antioxidant defenses, known as oxidative stress, can cause oxidation of biomolecules and further contribute to aging-associated diseases. The purpose of this study was to evaluate the antioxidant capacities of Thai traditional tonifyi...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-019-2626-1
更新日期:2019-08-13 00:00:00