Mining the deep Red-Sea brine pool microbial community for anticancer therapeutics.

Abstract:

BACKGROUND:Microbial species in the brine pools of the Red Sea and the brine pool-seawater interfaces are exposed to high temperature, high salinity, low oxygen levels and high concentrations of heavy metals. As adaptations to these harsh conditions require a large suite of secondary metabolites, these microbes have a huge potential as a source of novel anticancer molecules. METHODS:A total of 60 ethyl-acetate extracts of newly isolated strains from extreme environments of the Red-Sea were isolated and tested against several human cancer cell lines for potential cytotoxic and apoptotic activities. RESULTS:Isolates from the Erba brine-pool accounted for 50% of active bacterial extracts capable of inducing 30% or greater inhibition of cell growth. Among the 60 extracts screened, seven showed selectivity towards triple negative BT20 cells compared to normal fibroblasts. CONCLUSION:In this study, we identified several extracts able to induce caspase-dependent apoptosis in various cancer cell lines. Further investigations and isolation of the active compounds of these Red Sea brine pool microbes may offer a chemotherapeutic potential for cancers with limited treatment options.

authors

Esau L,Zhang G,Sagar S,Stingl U,Bajic VB,Kaur M

doi

10.1186/s12906-019-2554-0

subject

Has Abstract

pub_date

2019-06-20 00:00:00

pages

142

issue

1

issn

1472-6882

pii

10.1186/s12906-019-2554-0

journal_volume

19

pub_type

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