Abstract:
BACKGROUND:Ovarian cancer is associated with poor long-term survival due to late diagnosis and development of chemoresistance. Tumour hypoxia is associated with many features of tumour aggressiveness including increased cellular proliferation, inhibition of apoptosis, increased invasion and metastasis, and chemoresistance, mostly mediated through hypoxia-inducible factor (HIF)-1α. While HIF-1α has been associated with platinum resistance in a variety of cancers, including ovarian, relatively little is known about the importance of the duration of hypoxia. Similarly, the gene pathways activated in ovarian cancer which cause chemoresistance as a result of hypoxia are poorly understood. This study aimed to firstly investigate the effect of hypoxia duration on resistance to cisplatin in an ovarian cancer chemoresistance cell line model and to identify genes whose expression was associated with hypoxia-induced chemoresistance. METHODS:Cisplatin-sensitive (A2780) and cisplatin-resistant (A2780cis) ovarian cancer cell lines were exposed to various combinations of hypoxia and/or chemotherapeutic drugs as part of a 'hypoxia matrix' designed to cover clinically relevant scenarios in terms of tumour hypoxia. Response to cisplatin was measured by the MTT assay. RNA was extracted from cells treated as part of the hypoxia matrix and interrogated on Affymetrix Human Gene ST 1.0 arrays. Differential gene expression analysis was performed for cells exposed to hypoxia and/or cisplatin. From this, four potential markers of chemoresistance were selected for evaluation in a cohort of ovarian tumour samples by RT-PCR. RESULTS:Hypoxia increased resistance to cisplatin in A2780 and A2780cis cells. A plethora of genes were differentially expressed in cells exposed to hypoxia and cisplatin which could be associated with chemoresistance. In ovarian tumour samples, we found trends for upregulation of ANGPTL4 in partial responders and down-regulation in non-responders compared with responders to chemotherapy; down-regulation of HER3 in partial and non-responders compared to responders; and down-regulation of HIF-1α in non-responders compared with responders. CONCLUSION:This study has further characterized the relationship between hypoxia and chemoresistance in an ovarian cancer model. We have also identified many potential biomarkers of hypoxia and platinum resistance and provided an initial validation of a subset of these markers in ovarian cancer tissues.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
McEvoy LM,O'Toole SA,Spillane CD,Martin CM,Gallagher MF,Stordal B,Blackshields G,Sheils O,O'Leary JJdoi
10.1186/s12885-015-1539-8subject
Has Abstractpub_date
2015-07-25 00:00:00pages
547issn
1471-2407pii
10.1186/s12885-015-1539-8journal_volume
15pub_type
杂志文章相关文献
BMC CANCER文献大全abstract:BACKGROUND:The centralisation of specialist cancer surgical services across London Cancer and Greater Manchester Cancer, England, may significantly change how patients experience care. These centres are changing specialist surgical pathways for several cancers including prostate, bladder, kidney, and oesophago-gastric ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4137-8
更新日期:2018-02-27 00:00:00
abstract:BACKGROUND:Lifestyle factors, including food and nutrition, physical activity, body composition and reproductive factors, and single nucleotide polymorphisms (SNPs) are associated with breast cancer risk, but few studies of these factors have been performed in the Japanese population. Thus, the goals of this study were...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2407-13-565
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:Translationally controlled tumor protein (TCTP), alternatively called fortilin, is believed to be involved in the development of the chemoresistance of tumor cells against anticancer drugs such as etoposide, taxol, and oxaliplatin, the underlying mechanisms of which still remain elusive. METHODS:Cell death ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-165
更新日期:2014-03-07 00:00:00
abstract::Following publication of the original article [1], the authors reported an error in Fig. 6c and in the figure legends for Fig. 5c and Fig. 6c. ...
journal_title:BMC cancer
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12885-020-6552-x
更新日期:2020-01-23 00:00:00
abstract:BACKGROUND:To report a case of metastatic hepatocholangiocarcinoma to the vitreous and retina. CASE PRESENTATION:A 70-year-old male, who was recently diagnosed with hepatocholangiocarcinoma, was complaining of floaters in his right eye over the past 5 months and was referred to the Liverpool Ocular Oncology Centre. On...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3429-8
更新日期:2017-06-19 00:00:00
abstract:BACKGROUND:Maintenance of quality of life is the primary goal during treatment of brain metastases (BM). This is a protocol of an ongoing phase III randomised multicentre study. This study aims to determine the exact additional palliative value of stereotactic radiosurgery (SRS) over whole brain radiotherapy (WBRT) in ...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12885-017-3494-z
更新日期:2017-07-25 00:00:00
abstract:BACKGROUND:Primary angiosarcomas of the right atrium are extremely rare, often resulted in missed diagnosis or misdiagnosis with routine examination tools. These malignant cardiac tumors are highly aggressive with generally poor prognosis. Surgical excision is the mainstay of treatment as it is essentially not responsi...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-6450-2
更新日期:2020-01-06 00:00:00
abstract:BACKGROUND:Continued smoking after cancer adversely affects quality of life and survival, but one fifth of cancer survivors still smoke. Despite its demands, cancer presents an opportunity for positive behaviour change. Smoking often occurs in social groups, therefore interventions which target families and individuals...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3344-z
更新日期:2017-05-19 00:00:00
abstract:BACKGROUND:Ongoing improvements in technique and instruments for video-assisted thoracoscopic surgery (VATS) have made minimally-invasive uniportal VATS lobectomy a reality. However, the outcomes of the procedure are still under investigation, and at present, uniportal VATS lobectomy is performed infrequently at most h...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3069-z
更新日期:2017-01-25 00:00:00
abstract:BACKGROUND:Renal cell carcinoma (RCC) is a malignant disease that demonstrates resistance to standard chemotherapeutic agents. Yet Active immunization using genetically modified dendritic cells holds promise for the adjuvant treatment of malignancies to eradicate or control residual disease. Cytokine-induced killer (CI...
journal_title:BMC cancer
pub_type: 临床试验,杂志文章
doi:10.1186/1471-2407-14-251
更新日期:2014-04-10 00:00:00
abstract:BACKGROUND:Lung adenocarcinoma (LUAD) is a major cause of cancer death. Therefore, identifying potential prognostic risk factors is critical to improve the survival of patients with LUAD. METHODS:Here, relevant datasets were downloaded from TCGA and GEO databases to screen the differentially expressed genes (DEGs). Un...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07235-z
更新日期:2020-08-20 00:00:00
abstract:BACKGROUND:Invasive micropapillary carcinoma (IMPC) of the breast is a rare variant of invasive ductal carcinoma (IDC). The prognosis of IMPC compared with that of IDC remains controversial; we conducted a meta-analysis to evaluate the prognostic difference between IMPC and IDC. METHODS:We searched the PubMed, Cochran...
journal_title:BMC cancer
pub_type: 杂志文章,meta分析
doi:10.1186/s12885-017-3855-7
更新日期:2017-12-11 00:00:00
abstract:BACKGROUND:Infection with human papillomavirus (HPV) is necessary for the development of cervical carcinoma. By contrast, the role of HPV in the pathogenesis of other malignancies, such as head and neck cancers, is less well characterised. This study aimed to address key information gaps by conducting a systematic revi...
journal_title:BMC cancer
pub_type: 杂志文章,meta分析,评审
doi:10.1186/1471-2407-14-968
更新日期:2014-12-17 00:00:00
abstract:BACKGROUND:Since the second half of the 1990s, kidney cancer mortality has tended to stabilize and decline in many European countries, due to the decrease in the prevalence of smokers. Nevertheless, incidence of kidney cancer is rising across the sexes in some of these countries, a trend which may possibly reflect the ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-293
更新日期:2008-10-09 00:00:00
abstract:BACKGROUND:Durvalumab is a standard drug used during maintenance therapy after chemoradiotherapy in patients with locally advanced non-small cell lung cancer (LA-NSCLC). However, little is known about the clinical benefits of durvalumab after chemoradiotherapy in patients with LA-NSCLC with a performance status (PS) of...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07406-y
更新日期:2020-10-06 00:00:00
abstract:BACKGROUND:Ameloblastomas are odontogenic neoplasms characterized by local invasiveness. This study was conducted to address the role of matrix metalloproteinase-2 (MMP-2) in the invasiveness of ameloblastomas. METHODS:Plasmids containing either MMP-2 siRNA or tissue inhibitor of metalloproteinase-2 (TIMP-2) cDNA were...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-182
更新日期:2008-06-30 00:00:00
abstract:BACKGROUND:Stereotactic ablative body radiotherapy (SABR) is a non-invasive alternative to surgery to control primary renal cell cancer (RCC) in patients that are medically inoperable or at high-risk of post-surgical dialysis. The objective of the FASTRACK II clinical trial is to investigate the efficacy of SABR for pr...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4916-2
更新日期:2018-10-23 00:00:00
abstract:BACKGROUND:We utilized miRNAs expression and clinical data to develop a prognostic signature for patients with lung adenocarcinoma, with respect to their overall survival, to identify high-risk subjects based on their miRNA genomic profile. METHODS:MiRNA expressions based on miRNA sequencing and clinical data of lung ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-5206-8
更新日期:2019-01-08 00:00:00
abstract:BACKGROUND:Multi-drug Resistance associated Protein-1 (MRP1) can export chemotherapeutics from cancer cells and is implicated in chemoresistance, particularly as is it known to be up-regulated by chemotherapeutics. Our aims in this study were to determine whether activation of Notch signalling is responsible for chemot...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1625-y
更新日期:2015-09-11 00:00:00
abstract:BACKGROUND:Bevacizumab, an antibody neutralizing Vascular Endothelial Growth Factor (VEGF), is licensed for the management of patients with advanced colon cancer. However, tumor biomarkers identifying the molecular tumor subsets most amenable to angiogenesis modulation are lacking. METHODS:We profiled expession of 245...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2407-14-111
更新日期:2014-02-20 00:00:00
abstract::Autologous stem cell transplantation (ASCT) is a well-established approach to treatment of patients with relapsed/refractory (R/R) Hodgkin lymphoma (HL) recommended by both the European Society for Medical Oncology and the National Comprehensive Cancer Network based on the results from randomized controlled studies. H...
journal_title:BMC cancer
pub_type: 杂志文章,评审
doi:10.1186/s12885-020-07561-2
更新日期:2020-11-10 00:00:00
abstract:BACKGROUND:The development of metastases is a negative prognostic parameter for the clinical outcome of breast cancer. Bone constitutes the first site of distant metastases for many affected women. The purpose of this retrospective multicentre study was to evaluate if and how different variables such as primary tumour ...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/s12885-016-2345-7
更新日期:2016-05-12 00:00:00
abstract:BACKGROUND:The failure of treatment for breast cancer usually results from distant metastasis in which the epithelial-mesenchymal transition (EMT) plays a critical role. Hyperinsulinemia, the hallmark of Type 2 diabetes mellitus (T2DM), has been regarded as a key risk factor for the progression of breast cancer. Nuclea...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07107-6
更新日期:2020-07-06 00:00:00
abstract:BACKGROUND:Adoptive T-cell therapy relying on conventional T cells transduced with T-cell receptors (TCRs) or chimeric antigen receptors (CARs) has caused substantial tumor regression in several clinical trials. However, genetically engineered T cells have been associated with serious side-effects due to off-target tox...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3539-3
更新日期:2017-08-17 00:00:00
abstract:BACKGROUND:Obesity is linked to poor disease outcomes in breast cancer patients. However, this link was mostly based on body weight or BMI rather than body-fat. The purpose of this study was to evaluate the relationship between body-fat gain and disease progression in Taiwanese women after breast cancer surgery and how...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3869-1
更新日期:2017-12-18 00:00:00
abstract:BACKGROUND:5-fluorouracil, leucovorin, irinotecan and oxaliplatin (FOLFIRINOX) is superior to gemcitabine in patients with metastatic pancreatic cancer who have a good performance status. We investigated this combination as neoadjuvant therapy for locally advanced pancreatic cancer (LAPC). METHODS:In this retrospectiv...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-199
更新日期:2012-05-29 00:00:00
abstract:BACKGROUND:Aquaporins (AQPs) have been recognized to promote tumor progression, invasion, and metastasis and are therefore recognized as promising targets for novel anti-cancer therapies. Potentially relevant AQPs in distinct cancer entities can be determined by a comprehensive expression analysis of the 13 human AQPs....
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-161
更新日期:2011-05-06 00:00:00
abstract:BACKGROUND:Alteration in gut microbiota has been recently linked with childhood leukemia and the use of chemotherapy. Whether the perturbed microbiota community is restored after disease remission and cessation of cancer treatment has not been evaluated. This study examines the chronological changes of gut microbiota i...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-6654-5
更新日期:2020-02-24 00:00:00
abstract:BACKGROUND:Positron emission tomography (PET) and PET/computed tomography (PET/CT) imaging with 3,4-dihydroxy-6-[18F] fluoro-L-phenylalanine (18F-FDOPA) has been used in the evaluation of gliomas. We performed a meta-analysis to obtain the diagnostic and grading accuracy of 18F-FDOPA PET and PET/CT in patients with gli...
journal_title:BMC cancer
pub_type: 杂志文章,meta分析
doi:10.1186/s12885-019-5938-0
更新日期:2019-08-05 00:00:00
abstract:BACKGROUND:Increased level of serum S100B can serve as a marker of metastatic spread in patients with cutaneous melanoma (CM). In patients with elevated S100 B and/or clinical signs of disease progression PET-CT scan is a valuable tool for discovering metastases and planning treatment.The aims of this study were to det...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-328
更新日期:2011-08-02 00:00:00