Abstract:
BACKGROUND:The lungworm Dictyocaulus viviparus, causing parasitic bronchitis in cattle, induces a temporary protective immunity that prevents clinical disease. A radiation-attenuated larvae based vaccine is commercially available in a few European countries, but has the disadvantages of a live vaccine. As a recombinant subunit vaccine would overcome these disadvantages, the parasite's muscle protein paramyosin (PMY) was tested as a recombinant vaccine antigen. METHODS:D. viviparus-PMY was recombinantly expressed in Escherichia coli as a glutathione-S-transferase (GST)-fused protein. Emulsified in adjuvant Saponin Quil A, the protein was given intramuscularly into calves. Two independent recombinant PMY (rPMY) vaccination trials with negative control groups (first trial: adjuvant only; second trial: non-fused GST) as well as an additional positive control group in the second trial, using the Bovilis Dictol live vaccine to verify vaccination results, were performed. To determine the vaccination success, shedding of larvae as well as worm burden and worm sizes were analyzed. Additionally, ELISA-based determination of development of immunglobulins IgM, IgA, IgE, IgG as well as the subclasses IgG1 and IgG2 was performed. To analyze PMY localization in the bovine lungworm, immunohistochemical staining of adult worms was carried out. RESULTS:Immunohistochemical staining revealed that PMY is part of the bovine lungworm's pharyngeal and body wall muscles. Vaccination with rPMY resulted in 47% [geometric mean: 67%] and 57% (geometric mean: 71%) reduction of larvae shedding in the first and second vaccination trial, respectively. Worm burden was reduced by 54% (geometric mean: 86%) and 31% (geometric mean: 68%), respectively, and worms of rPMY-vaccinated cattle were significantly shorter in both trials. Furthermore, ELISAs showed a clear antibody response towards rPMY with exception of IgE for which titers could not be detected. After challenge infection, rPMY antibodies were only exceptionally elevated among study animals indicating PMY to be a hidden antigen. CONCLUSIONS:Even though vaccination with the attenuated live vaccine was with 94% (geometric mean: 95%) reduction in larvae shedding and 93% (geometric mean: 94%) reduction in worm burden superior to rPMY vaccination, results using the latter are promising and show the potential for further development of a recombinant PMY-based vaccine against the bovine lungworm.
journal_name
Parasit Vectorsjournal_title
Parasites & vectorsauthors
Strube C,Haake C,Sager H,Schorderet Weber S,Kaminsky R,Buschbaum S,Joekel D,Schicht S,Kremmer E,Korrell J,Schnieder T,von Samson-Himmelstjerna Gdoi
10.1186/s13071-015-0733-5subject
Has Abstractpub_date
2015-02-24 00:00:00pages
119issn
1756-3305pii
10.1186/s13071-015-0733-5journal_volume
8pub_type
杂志文章abstract:BACKGROUND:Anopheles mosquitoes transmit malaria which is one of the world's most threatening diseases. Anopheles dirus (sensu stricto) is among the main vectors of malaria in South East Asia. The mosquito innate immune response is the first line of defence against malaria parasites during its development. The immune d...
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pub_type: 杂志文章
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
pub_type: 杂志文章,随机对照试验
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journal_title:Parasites & vectors
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更新日期:2014-03-19 00:00:00
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
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更新日期:2016-06-27 00:00:00
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journal_title:Parasites & vectors
pub_type: 杂志文章
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journal_title:Parasites & vectors
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journal_title:Parasites & vectors
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更新日期:2013-05-24 00:00:00
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更新日期:2012-08-31 00:00:00
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journal_title:Parasites & vectors
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