当前位置： SCI文献检索 > Parasites & Vectors期刊下所有文献 > Trypanosome infections in naturally infected horses and donkeys of three active sleeping sickness foci in the south of Chad.

Trypanosome infections in naturally infected horses and donkeys of three active sleeping sickness foci in the south of Chad.

Abstract：

BACKGROUND:Equine trypanosomiases are complex infectious diseases with overlapping clinical signs defined by their mode of transmission. Despite their economic impacts, these diseases have been neglected by the scientific community, the veterinary authorities and regulatory organizations. To fill the observed knowledge gap, we undertook the identification of different trypanosome species and subspecies naturally infecting horses and donkeys within the Chadian sleeping sickness focus. The objective of the study was to investigate the potential role of these domestic animals as reservoirs of the human-infective Trypanosoma brucei gambiense. METHOD:Blood samples were collected from 155 donkeys and 131 horses in three human African trypanosomiasis (HAT) foci in Chad. Rapid diagnostic test (RDT) and capillary tube centrifugation (CTC) test were used to search for trypanosome infections. DNA was extracted from each blood sample and different trypanosome species and subspecies were identified with molecular tools. RESULTS:From 286 blood samples collected, 54 (18.9%) and 36 (12.6%) were positive for RDT and CTC, respectively. PCR revealed 101 (35.3%) animals with trypanosome infections. The Cohen's kappa coefficient used to evaluate the concordance between the diagnostic methods were low; ranging from 0.09 ± 0.05 to 0.48 ± 0.07. Trypanosomes of the subgenus Trypanozoon were the most prevalent (29.4%), followed by T. congolense forest (11.5%), Trypanosoma congolense savannah (4.9%) and Trypanosoma vivax (4.5%). Two donkeys and one horse from the Maro HAT focus were found with T. b. gambiense infections. No significant differences were observed in the infection rates of different trypanosomes between animal species and HAT foci. CONCLUSIONS:This study revealed several trypanosome species and subspecies in donkeys and horses, highlighting the existence of AAT in HAT foci in Chad. The identification of T. b. gambiense in donkeys and horses suggests considering these animals as potential reservoir for HAT in Chad. The presence of both human-infective and human non-infective trypanosomes species highlights the need for developing joint control strategies for HAT and AAT.

journal_name

Parasit Vectors

journal_title

Parasites & vectors

authors

Vourchakbé J,Tiofack AAZ,Mbida M,Simo G

doi

10.1186/s13071-020-04192-1

subject

Has Abstract

pub_date

2020-06-23 00:00:00

pages

323

issue

issn

1756-3305

pii

10.1186/s13071-020-04192-1

journal_volume

pub_type

杂志文章

在线工具

Trypanosome infections in naturally infected horses and donkeys of three active sleeping sickness foci in the south of Chad.