Collaborative interactions between neutrophil elastase and metalloproteinases in extracellular matrix degradation in three-dimensional collagen gels.


BACKGROUND:Extended culture of monocytes and fibroblasts in three-dimensional collagen gels leads to degradation of the gels (see linked study in this issue, "Fibroblasts and monocytes contract and degrade three-dimensional collagen gels in extended co-culture"). The current study, therefore, was designed to evaluate production of matrix-degrading metalloproteinases by these cells in co-culture and to determine if neutrophil elastase could collaborate in the activation of these enzymes. Since co-cultures produce prostaglandin E2 (PGE2), the role of PGE2 in this process was also evaluated. METHODS:Blood monocytes from healthy donors and human fetal lung fibroblasts were cast into type I collagen gels and maintained in floating cultures for three weeks. Matrix metalloproteinases (MMPs) were assessed by gelatin zymography (MMPs 2 and 9) and immunoblotting (MMPs 1 and 3). The role of PGE2 was explored by direct quantification, and by the addition of exogenous indomethacin and/or PGE2. RESULTS:Gelatin zymography and immunoblots revealed that MMPs 1, 2, 3 and 9 were induced by co-cultures of fibroblasts and monocytes. Neutrophil elastase added to the medium resulted in marked conversion of latent MMPs to lower molecular weight forms consistent with active MMPs, and was associated with augmentation of both contraction and degradation (P < 0.01). PGE2 appeared to decrease both MMP production and activation. CONCLUSION:The current study demonstrates that interactions between monocytes and fibroblasts can mediate tissue remodeling.


Respir Res


Respiratory research


Zhu Y,Liu X,Sköld CM,Wang H,Kohyama T,Wen FQ,Ertl RF,Rennard SI





Has Abstract


2001-01-01 00:00:00












  • COPD diagnosis related to different guidelines and spirometry techniques.

    abstract::The aim was to compare the diagnosis of COPD among smokers according to different international guidelines and to compare the outcome when using slow (SVC) and forced vital capacity (FVC). In order to find current smokers a questionnaire was sent to persons who had been on sick leave for more than two weeks. Those who...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Nathell L,Nathell M,Malmberg P,Larsson K

    更新日期:2007-12-04 00:00:00

  • Characteristics, disease burden and costs of COPD patients in the two years following initiation of long-acting bronchodilators in UK primary care.

    abstract:BACKGROUND:To assess the symptomatic and cost burden among patients initiating long-acting bronchodilator (LABD) therapy and impact of adherence on healthcare resource use and costs. METHODS:This retrospective cohort study identified patients with COPD who were newly prescribed a LABD (long-acting muscarinic antagonis...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Punekar YS,Landis SH,Wurst K,Le H

    更新日期:2015-11-16 00:00:00

  • Protein tyrosine phosphatase PTP-RR regulates corticosteroid sensitivity.

    abstract:BACKGROUND:We have recently reported that protein phosphate 2A (PP2A) inactivation resulted in increased phosphorylation of the mitogen-activated protein kinase (MAPK) c-Jun N-terminal kinase 1 (JNK1) and glucocorticoid receptors (GR) at Ser(226), thereby reducing GR nuclear translocation and causing corticosteroid ins...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Kobayashi Y,Ito K,Kanda A,Tomoda K,Miller-Larsson A,Barnes PJ,Mercado N

    更新日期:2016-03-24 00:00:00

  • Electronic cigarette vapour increases virulence and inflammatory potential of respiratory pathogens.

    abstract:INTRODUCTION:Bacteria have been extensively implicated in the development of smoking related diseases, such as COPD, by either direct infection or bacteria-mediated inflammation. In response to the health risks associated with tobacco exposure, the use of electronic cigarettes (e-cigs) has increased. This study compare...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Gilpin DF,McGown KA,Gallagher K,Bengoechea J,Dumigan A,Einarsson G,Elborn JS,Tunney MM

    更新日期:2019-12-18 00:00:00

  • Corticosteroid suppression of lipoxin A4 and leukotriene B4 from alveolar macrophages in severe asthma.

    abstract:BACKGROUND:An imbalance in the generation of pro-inflammatory leukotrienes, and counter-regulatory lipoxins is present in severe asthma. We measured leukotriene B4 (LTB4), and lipoxin A4 (LXA4) production by alveolar macrophages (AMs) and studied the impact of corticosteroids. METHODS:AMs obtained by fiberoptic bronch...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Bhavsar PK,Levy BD,Hew MJ,Pfeffer MA,Kazani S,Israel E,Chung KF

    更新日期:2010-06-07 00:00:00

  • Genes associated with polymorphic variants predicting lung function are differentially expressed during human lung development.

    abstract:BACKGROUND:Recent meta-analyses of genome-wide association studies have identified single nucleotide polymorphisms (SNPs) within/near 54 genes associated with lung function measures. Current understanding of the contribution of these genes to human lung development is limited. We set out to further define i) the expres...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Miller S,Melén E,Merid SK,Hall IP,Sayers I

    更新日期:2016-07-29 00:00:00

  • Activation of the nicotinamide N-methyltransferase (NNMT)-1-methylnicotinamide (MNA) pathway in pulmonary hypertension.

    abstract:BACKGROUND:Pulmonary arterial hypertension (PAH) is associated with inflammatory response but it is unknown whether it is associated with alterations in NNMT activity and MNA plasma concentration. Here we examined changes in NNMT-MNA pathway in PAH in rats and humans. METHODS:PAH in rats was induced by a single subcut...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Fedorowicz A,Mateuszuk Ł,Kopec G,Skórka T,Kutryb-Zając B,Zakrzewska A,Walczak M,Jakubowski A,Łomnicka M,Słomińska E,Chlopicki S

    更新日期:2016-08-31 00:00:00

  • Rate of progression of CT-quantified emphysema in male current and ex-smokers: a follow-up study.

    abstract:BACKGROUND:Little is known about the factors associated with CT-quantified emphysema progression in heavy smokers. The objective of this study was to investigate the effect of length of smoking cessation and clinical / demographical factors on the rate of emphysema progression and FEV1-decline in male heavy smokers. M...

    journal_title:Respiratory research

    pub_type: 杂志文章,随机对照试验


    authors: Mohamed Hoesein FA,Zanen P,de Jong PA,van Ginneken B,Boezen HM,Groen HJ,Oudkerk M,de Koning HJ,Postma DS,Lammers JW

    更新日期:2013-05-20 00:00:00

  • The anti-proliferative and anti-inflammatory response of COPD airway smooth muscle cells to hydrogen sulfide.

    abstract:BACKBROUND:COPD is a common, highly debilitating disease of the airways, primarily caused by smoking. Chronic inflammation and structural remodelling are key pathological features of this disease caused, in part, by the aberrant function of airway smooth muscle (ASM). We have previously demonstrated that hydrogen sulfi...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Perry MM,Tildy B,Papi A,Casolari P,Caramori G,Rempel KL,Halayko AJ,Adcock I,Chung KF

    更新日期:2018-05-09 00:00:00

  • Profibrotic role of WNT10A via TGF-β signaling in idiopathic pulmonary fibrosis.

    abstract:BACKGROUND:WNT/β-catenin signaling plays an important role in the pathogenesis of idiopathic pulmonary fibrosis (IPF); however, the role of WNT10A via transforming growth factor (TGF)-β signaling remains unclear. METHODS:We evaluated the expression of WNT10A and TGF-β in bleomycin (BLM)-treated mice and the interactio...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Oda K,Yatera K,Izumi H,Ishimoto H,Yamada S,Nakao H,Hanaka T,Ogoshi T,Noguchi S,Mukae H

    更新日期:2016-04-12 00:00:00

  • Exogenous interleukin-6, interleukin-13, and interferon-γ provoke pulmonary abnormality with mild edema in enterovirus 71-infected mice.

    abstract:BACKGROUND:Neonatal mice developed neurological disease and pulmonary dysfunction after an infection with a mouse-adapted human Enterovirus 71 (EV71) strain MP4. However, the hallmark of severe human EV71 infection, pulmonary edema (PE), was not evident. METHODS:To test whether EV71-induced PE required a proinflammato...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Huang SW,Lee YP,Hung YT,Lin CH,Chuang JI,Lei HY,Su IJ,Yu CK

    更新日期:2011-11-06 00:00:00

  • CD8+ T lymphocytes in lung tissue from patients with idiopathic pulmonary fibrosis.

    abstract:BACKGROUND:Several studies have implicated a role of inflammation in the pathogenesis of lung damage in idiopathic pulmonary fibrosis (IPF). Parenchymal lung damage leads to defects in mechanics and gas exchange and clinically manifests with exertional dyspnea. Investigations of inflammatory cells in IPF have shown tha...

    journal_title:Respiratory research

    pub_type: 临床试验,杂志文章


    authors: Daniil Z,Kitsanta P,Kapotsis G,Mathioudaki M,Kollintza A,Karatza M,Milic-Emili J,Roussos C,Papiris SA

    更新日期:2005-07-24 00:00:00

  • Endotoxin receptor CD14 in PiZ alpha-1-antitrypsin deficiency individuals.

    abstract:BACKGROUND:CD14, a receptor for lipopolysaccharides (LPS), is found in both a membrane-bound form (mCD14) and a soluble form (sCD14). It is suggested that sCD14 is mainly released from blood monocytes by serine protease-mediated shedding. Because alpha1-antitrypsin (AAT), an inhibitor of serine proteases, has been show...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Sandström CS,Novoradovskaya N,Cilio CM,Piitulainen E,Sveger T,Janciauskiene S

    更新日期:2008-04-21 00:00:00

  • Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells.

    abstract:BACKGROUND:The mechanisms during the initial phase of oxygen toxicity leading to pulmonary tissue damage are incompletely known. Increase of tumour necrosis factor alpha (TNFalpha) represents one of the first pulmonary responses to hyperoxia. We hypothesised that, in the initial phase of hyperoxia, TNFalpha activates t...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Guthmann F,Wissel H,Schachtrup C,Tölle A,Rüdiger M,Spener F,Rüstow B

    更新日期:2005-01-21 00:00:00

  • Pathophysiological classification of chronic rhinosinusitis.

    abstract:BACKGROUND:Recent consensus statements demonstrate the breadth of the chronic rhinosinusitis (CRS) differential diagnosis. However, the classification and mechanisms of different CRS phenotypes remains problematic. METHOD:Statistical patterns of subjective and objective findings were assessed by retrospective chart re...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Baraniuk JN,Maibach H

    更新日期:2005-12-19 00:00:00

  • Impact of gastroesophageal reflux on longitudinal lung function and quantitative computed tomography in the COPDGene cohort.

    abstract:RATIONALE:Gastroesophageal reflux disease (GERD) is a common comorbidity in chronic obstructive pulmonary disease (COPD) and has been associated with increased risk of acute exacerbations, hospitalization, emergency room visits, costs, and quality-of-life impairment. However, it remains unclear whether GERD contributes...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Baldomero AK,Wendt CH,Petersen A,Gaeckle NT,Han MK,Kunisaki KM,COPDGene Investigators.

    更新日期:2020-08-03 00:00:00

  • Perforin, granzyme B, and FasL expression by peripheral blood T lymphocytes in emphysema.

    abstract:BACKGROUND:It is generally accepted that emphysematous lungs are characterized by an increase in the numbers of neutrophils, macrophages, and CD8+ T lymphocytes, the lasts having increased cytotoxic activity. Because systemic inflammation is also a component of emphysema, we hypothesize that peripheral CD8+ T lymphocyt...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Morissette MC,Parent J,Milot J

    更新日期:2007-09-06 00:00:00

  • Serum biomarkers in interstitial lung diseases.

    abstract::The use of biomarkers in medicine lies in their ability to detect disease and support diagnostic and therapeutic decisions. New research and novel understanding of the molecular basis of the disease reveals an abundance of exciting new biomarkers who present a promise for use in the everyday clinical practice. The pas...

    journal_title:Respiratory research

    pub_type: 杂志文章,评审


    authors: Tzouvelekis A,Kouliatsis G,Anevlavis S,Bouros D

    更新日期:2005-07-21 00:00:00

  • The Tiotropium Safety and Performance in Respimat Trial (TIOSPIR), a large scale, randomized, controlled, parallel-group trial-design and rationale.

    abstract:BACKGROUND:Tiotropium bromide is an effective therapy for COPD patients. Comparing across programs tiotropium Respimat Soft Mist inhaler was at least as efficacious as tiotropium HandiHaler, however, concerns have been raised about tiotropium's safety when given via Respimat. METHODS:The TIOSPIR trial (NCT01126437) co...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Wise RA,Anzueto A,Calverley P,Dahl R,Dusser D,Pledger G,Koenen-Bergmann M,Joseph E,Cotton D,Disse B

    更新日期:2013-04-02 00:00:00

  • Artificial intelligence accuracy in detecting pathological breath sounds in children using digital stethoscopes.

    abstract:BACKGROUND:Manual auscultation to detect abnormal breath sounds has poor inter-observer reliability. Digital stethoscopes with artificial intelligence (AI) could improve reliable detection of these sounds. We aimed to independently test the abilities of AI developed for this purpose. METHODS:One hundred and ninety two...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Kevat A,Kalirajah A,Roseby R

    更新日期:2020-09-29 00:00:00

  • Dibutyryl-cAMP attenuates pulmonary fibrosis by blocking myofibroblast differentiation via PKA/CREB/CBP signaling in rats with silicosis.

    abstract:BACKGROUND:Myofibroblasts play a major role in the synthesis of extracellular matrix (ECM) and the stimulation of these cells is thought to play an important role in the development of silicosis. The present study was undertaken to investigate the anti-fibrotic effects of dibutyryl-cAMP (db-cAMP) on rats induced by sil...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Liu Y,Xu H,Geng Y,Xu D,Zhang L,Yang Y,Wei Z,Zhang B,Li S,Gao X,Wang R,Zhang X,Brann D,Yang F

    更新日期:2017-02-21 00:00:00

  • Psychometric properties and minimal important differences of SF-36 in Idiopathic Pulmonary Fibrosis.

    abstract:BACKGROUND:Idiopathic pulmonary fibrosis (IPF) is a rare disease with a median survival of 3-5 years after diagnosis with limited treatment options. The aim of this study is to assess the psychometric characteristics of the Short Form 36 Health Status Questionnaire (SF-36) in IPF and to provide disease specific minimal...

    journal_title:Respiratory research

    pub_type: 临床试验,杂志文章


    authors: Witt S,Krauss E,Barbero MAN,Müller V,Bonniaud P,Vancheri C,Wells AU,Vasakova M,Pesci A,Klepetko W,Seeger W,Crestani B,Leidl R,Holle R,Schwarzkopf L,Guenther A

    更新日期:2019-03-01 00:00:00

  • Five microRNAs in plasma as novel biomarkers for screening of early-stage non-small cell lung cancer.

    abstract:BACKGROUND:In order to find novel noninvasive biomarkers with high accuracy for the screening of early-stage non-small cell lung cancer (NSCLC), we investigate the predictive power of 5 microRNAs (miR-20a, miR-145, miR-21, miR223 and miR-221) as potential biomarkers in early-stage NSCLC. METHODS:In training set, 25 ea...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Geng Q,Fan T,Zhang B,Wang W,Xu Y,Hu H

    更新日期:2014-11-25 00:00:00

  • Derivation of therapeutic lung spheroid cells from minimally invasive transbronchial pulmonary biopsies.

    abstract:BACKGROUND:Resident stem and progenitor cells have been identified in the lung over the last decade, but isolation and culture of these cells remains a challenge. Thus, although these lung stem and progenitor cells provide an ideal source for stem-cell based therapy, mesenchymal stem cells (MSCs) remain the most popula...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Dinh PC,Cores J,Hensley MT,Vandergriff AC,Tang J,Allen TA,Caranasos TG,Adler KB,Lobo LJ,Cheng K

    更新日期:2017-06-30 00:00:00

  • Individuals with increased inflammatory response to ozone demonstrate muted signaling of immune cell trafficking pathways.

    abstract:BACKGROUND:Exposure to ozone activates innate immune function and causes neutrophilic (PMN) airway inflammation that in some individuals is robustly elevated. The interplay between immuno-inflammatory function and genomic signaling in those with heightened inflammatory responsiveness to ozone is not well understood. O...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Fry RC,Rager JE,Zhou H,Zou B,Brickey JW,Ting J,Lay JC,Peden DB,Alexis NE

    更新日期:2012-10-03 00:00:00

  • CCR2 and CXCR3 agonistic chemokines are differently expressed and regulated in human alveolar epithelial cells type II.

    abstract::The attraction of leukocytes from circulation to inflamed lungs depends on the activation of both the leukocytes and the resident cells within the lung. In this study we determined gene expression and secretion patterns for monocyte chemoattractant protein-1 (MCP-1/CCL2) and T-cell specific CXCR3 agonistic chemokines ...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Pechkovsky DV,Goldmann T,Ludwig C,Prasse A,Vollmer E,Müller-Quernheim J,Zissel G

    更新日期:2005-07-20 00:00:00

  • Bidirectional role of IL-6 signal in pathogenesis of lung fibrosis.

    abstract:BACKGROUND:Various signals are known to participate in the pathogenesis of lung fibrosis. Our aim was to determine which signal is predominantly mobilized in the early inflammatory phase and thereafter modulates the development of lung fibrosis. METHODS:Mice received a single dose of 3 mg/kg body weight of bleomycin (...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Kobayashi T,Tanaka K,Fujita T,Umezawa H,Amano H,Yoshioka K,Naito Y,Hatano M,Kimura S,Tatsumi K,Kasuya Y

    更新日期:2015-08-20 00:00:00

  • Azithromycin and cough-specific health status in patients with chronic obstructive pulmonary disease and chronic cough: a randomised controlled trial.

    abstract:BACKGROUND:Macrolides reduce exacerbations in patients with COPD. Their effects on health status has not been assessed as primary outcome and is less clear. This study assessed the effects of prophylactic azithromycin on cough-specific health status in COPD-patients with chronic productive cough. METHODS:In this rando...

    journal_title:Respiratory research

    pub_type: 杂志文章,随机对照试验


    authors: Berkhof FF,Doornewaard-ten Hertog NE,Uil SM,Kerstjens HA,van den Berg JW

    更新日期:2013-11-14 00:00:00

  • Landscape of transcription and long non-coding RNAs reveals new insights into the inflammatory and fibrotic response following ventilator-induced lung injury.

    abstract:BACKGROUND:Mechanical ventilation can cause ventilator-induced lung injury (VILI) and lung fibrosis; however, the underlying mechanisms are still not fully understood. RNA sequencing is a powerful means for detecting vitally important protein-coding transcripts and long non-coding RNAs (lncRNAs) on a genome-wide scale,...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Wang L,Zhang N,Zhang Y,Xia J,Zhan Q,Wang C

    更新日期:2018-06-22 00:00:00

  • Indacaterol/glycopyrronium is cost-effective compared to salmeterol/fluticasone in COPD: FLAME-based modelling in a Swedish population.

    abstract:BACKGROUND:This study assessed the cost-effectiveness of indacaterol/glycopyrronium (IND/GLY) versus salmeterol/fluticasone (SFC) in chronic obstructive pulmonary disease (COPD) patients with moderate to very severe airflow limitation and ≥1 exacerbation in the preceding year. METHODS:A previously published and valida...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Bjermer L,van Boven JFM,Costa-Scharplatz M,Keininger DL,Gutzwiller FS,Lisspers K,Mahon R,Olsson P,Roche N

    更新日期:2017-12-11 00:00:00