LPS induces IL-10 production by human alveolar macrophages via MAPKinases- and Sp1-dependent mechanisms.


BACKGROUND:IL-10 is a cytokine mainly produced by macrophages that plays key roles in tolerance to inhaled antigens and in lung homeostasis. Its regulation in alveolar macrophages (HAM), the resident lung phagocytes, remains however unknown. METHODS:The present study investigated the role of intracellular signalling and transcription factors controlling the production of IL-10 in LPS-activated HAM from normal nonsmoking volunteers. RESULTS:LPS (1-1000 pg/ml) induced in vitro IL-10 production by HAM, both at mRNA and protein levels. LPS also activated the phosphorylation of ERK, p38 and JNK MAPkinases (immunoblots) and Sp-1 nuclear activity (EMSA). Selective inhibitors of MAPKinases (respectively PD98059, SB203580 and SP600125) and of Sp-1 signaling (mithramycin) decreased IL-10 expression in HAM. In addition, whilst not affecting IL-10 mRNA degradation, the three MAPKinase inhibitors completely abolished Sp-1 activation by LPS in HAM. CONCLUSION:These results demonstrate for the first time that expression of IL-10 in lung macrophages stimulated by LPS depends on the concomitant activation of ERK, p38 and JNK MAPKinases, which control downstream signalling to Sp-1 transcription factor. This study further points to Sp-1 as a key signalling pathway for IL-10 expression in the lung.


Respir Res


Respiratory research


Chanteux H,Guisset AC,Pilette C,Sibille Y




Has Abstract


2007-10-04 00:00:00












  • Electronic cigarette vapour increases virulence and inflammatory potential of respiratory pathogens.

    abstract:INTRODUCTION:Bacteria have been extensively implicated in the development of smoking related diseases, such as COPD, by either direct infection or bacteria-mediated inflammation. In response to the health risks associated with tobacco exposure, the use of electronic cigarettes (e-cigs) has increased. This study compare...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Gilpin DF,McGown KA,Gallagher K,Bengoechea J,Dumigan A,Einarsson G,Elborn JS,Tunney MM

    更新日期:2019-12-18 00:00:00

  • Cleaved cytokeratin-18 is a mechanistically informative biomarker in idiopathic pulmonary fibrosis.

    abstract:BACKGROUND:Stress of the endoplasmic reticulum (ER) leading to activation of the unfolded protein response (UPR) and alveolar epithelial cell (AEC) apoptosis may play a role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). Our objectives were to determine whether circulating caspase-cleaved cytokeratin-18 (c...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Cha SI,Ryerson CJ,Lee JS,Kukreja J,Barry SS,Jones KD,Elicker BM,Kim DS,Papa FR,Collard HR,Wolters PJ

    更新日期:2012-11-20 00:00:00

  • Weighing the evidence for pharmacological treatment interventions in mild COPD; a narrative perspective.

    abstract::There is increasing focus on understanding the nature of chronic obstructive pulmonary disease (COPD) during the earlier stages. Mild COPD (Global Initiative for Chronic Obstructive Lung Disease [GOLD] stage 1 or the now-withdrawn GOLD stage 0) represents an early stage of COPD that may progress to more severe disease...

    journal_title:Respiratory research

    pub_type: 杂志文章,评审


    authors: Singh D,D'Urzo AD,Donohue JF,Kerwin EM

    更新日期:2019-07-08 00:00:00

  • Different effects of deep inspirations on central and peripheral airways in healthy and allergen-challenged mice.

    abstract:BACKGROUND:Deep inspirations (DI) have bronchodilatory and bronchoprotective effects in healthy human subjects, but these effects appear to be absent in asthmatic lungs. We have characterized the effects of DI on lung mechanics during mechanical ventilation in healthy mice and in a murine model of acute and chronic air...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Jonasson S,Swedin L,Lundqvist M,Hedenstierna G,Dahlén SE,Hjoberg J

    更新日期:2008-02-28 00:00:00

  • Landscape of transcription and long non-coding RNAs reveals new insights into the inflammatory and fibrotic response following ventilator-induced lung injury.

    abstract:BACKGROUND:Mechanical ventilation can cause ventilator-induced lung injury (VILI) and lung fibrosis; however, the underlying mechanisms are still not fully understood. RNA sequencing is a powerful means for detecting vitally important protein-coding transcripts and long non-coding RNAs (lncRNAs) on a genome-wide scale,...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Wang L,Zhang N,Zhang Y,Xia J,Zhan Q,Wang C

    更新日期:2018-06-22 00:00:00

  • The Tiotropium Safety and Performance in Respimat Trial (TIOSPIR), a large scale, randomized, controlled, parallel-group trial-design and rationale.

    abstract:BACKGROUND:Tiotropium bromide is an effective therapy for COPD patients. Comparing across programs tiotropium Respimat Soft Mist inhaler was at least as efficacious as tiotropium HandiHaler, however, concerns have been raised about tiotropium's safety when given via Respimat. METHODS:The TIOSPIR trial (NCT01126437) co...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Wise RA,Anzueto A,Calverley P,Dahl R,Dusser D,Pledger G,Koenen-Bergmann M,Joseph E,Cotton D,Disse B

    更新日期:2013-04-02 00:00:00

  • Body composition and functional limitation in COPD.

    abstract:BACKGROUND:Low body mass index has been associated with increased mortality in severe COPD. The impact of body composition earlier in the disease remains unclear. We studied the impact of body composition on the risk of functional limitation in COPD. METHODS:We used bioelectrical impedance to estimate body composition...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Eisner MD,Blanc PD,Sidney S,Yelin EH,Lathon PV,Katz PP,Tolstykh I,Ackerson L,Iribarren C

    更新日期:2007-01-29 00:00:00

  • Role of PM2.5 in the development and progression of COPD and its mechanisms.

    abstract:BACKGROUND:A multitude of epidemiological studies have shown that ambient fine particulate matter 2.5 (diameter < 2.5um; PM2.5) was associated with increased morbidity and mortality of chronic obstructive pulmonary disease (COPD). However, the underlying associated mechanisms have not yet been elucidated. We conducted ...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Zhao J,Li M,Wang Z,Chen J,Zhao J,Xu Y,Wei X,Wang J,Xie J

    更新日期:2019-06-13 00:00:00

  • Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells.

    abstract:BACKGROUND:The mechanisms during the initial phase of oxygen toxicity leading to pulmonary tissue damage are incompletely known. Increase of tumour necrosis factor alpha (TNFalpha) represents one of the first pulmonary responses to hyperoxia. We hypothesised that, in the initial phase of hyperoxia, TNFalpha activates t...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Guthmann F,Wissel H,Schachtrup C,Tölle A,Rüdiger M,Spener F,Rüstow B

    更新日期:2005-01-21 00:00:00

  • Sex-specific lung functional changes in adult mice exposed only to second-hand smoke in utero.

    abstract:BACKGROUND:An increasing number of epidemiological and experimental studies have associated exposure to second-hand smoke (SHS) during pregnancy with adverse outcomes in newborns. As we have previously shown in mice, in utero exposure to SHS at critical stages of fetal development, results in altered lung responses and...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Noël A,Xiao R,Perveen Z,Zaman H,Le Donne V,Penn A

    更新日期:2017-06-27 00:00:00

  • Pentraxin 3 promotes airway inflammation in experimental asthma.

    abstract:BACKGROUND:Pentraxin 3 (PTX3) regulates multiple aspects of innate immunity and tissue inflammation. Recently, it has been reported that PTX3 deficiency enhances interleukin (IL)-17A-dominant pulmonary inflammation in an ovalbumin (OVA)-induced mouse asthma model. However, whether PTX3 treatment would provide protectio...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Gao P,Tang K,Lu Y,Huang Z,Wang S,Wang M,Wang J,Zhao J,Xie J

    更新日期:2020-09-16 00:00:00

  • Role of TNF-alpha in lung tight junction alteration in mouse model of acute lung inflammation.

    abstract::In the present study, we used tumor necrosis factor-R1 knock out mice (TNF-alpha R1KO) to understand the roles of TNF-alpha on epithelial function in models of carrageenan-induced acute lung inflammation. In order to elucidate whether the observed anti-inflammatory status is related to the inhibition of TNF-alpha, we ...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Mazzon E,Cuzzocrea S

    更新日期:2007-10-30 00:00:00

  • Surfactant protein D attenuates sub-epithelial fibrosis in allergic airways disease through TGF-β.

    abstract:BACKGROUND:Surfactant protein D (SP-D) can regulate both innate and adaptive immunity. Recently, SP-D has been shown to contribute to the pathogenesis of airway allergic inflammation and bleomycin-induced pulmonary fibrosis. However, in allergic airways disease, the role of SP-D in airway remodeling remains unknown. Th...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Ogawa H,Ledford JG,Mukherjee S,Aono Y,Nishioka Y,Lee JJ,Izumi K,Hollingsworth JW

    更新日期:2014-11-29 00:00:00

  • The anti-proliferative and anti-inflammatory response of COPD airway smooth muscle cells to hydrogen sulfide.

    abstract:BACKBROUND:COPD is a common, highly debilitating disease of the airways, primarily caused by smoking. Chronic inflammation and structural remodelling are key pathological features of this disease caused, in part, by the aberrant function of airway smooth muscle (ASM). We have previously demonstrated that hydrogen sulfi...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Perry MM,Tildy B,Papi A,Casolari P,Caramori G,Rempel KL,Halayko AJ,Adcock I,Chung KF

    更新日期:2018-05-09 00:00:00

  • Role of ATP-sensitive potassium channels on hypoxic pulmonary vasoconstriction in endotoxemia.

    abstract:BACKGROUND:ATP-regulated potassium channels (KATP) regulate pulmonary vascular tone and are involved in hypoxic pulmonary vasoconstriction (HPV). In patients with inflammation like sepsis or ARDS, HPV is impaired, resulting in a ventilation-perfusion mismatch and hypoxia. Since increase of vascular KATP channel Kir6.1 ...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Turzo M,Vaith J,Lasitschka F,Weigand MA,Busch CJ

    更新日期:2018-02-13 00:00:00

  • Laminin-332 alters connexin profile, dye coupling and intercellular Ca2+ waves in ciliated tracheal epithelial cells.

    abstract:BACKGROUND:Tracheal epithelial cells are anchored to a dynamic basement membrane that contains a variety of extracellular matrix proteins including collagens and laminins. During development, wound repair and disease of the airway epithelium, significant changes in extracellular matrix proteins may directly affect cell...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Isakson BE,Olsen CE,Boitano S

    更新日期:2006-08-02 00:00:00

  • Arginase strongly impairs neuronal nitric oxide-mediated airway smooth muscle relaxation in allergic asthma.

    abstract:BACKGROUND:Using guinea pig tracheal preparations, we have recently shown that endogenous arginase activity attenuates inhibitory nonadrenergic noncholinergic (iNANC) nerve-mediated airway smooth muscle relaxation by reducing nitric oxide (NO) production--due to competition with neuronal NO-synthase (nNOS) for the comm...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Maarsingh H,Leusink J,Bos IS,Zaagsma J,Meurs H

    更新日期:2006-01-12 00:00:00

  • Regenerative defect in vastus lateralis muscle of patients with chronic obstructive pulmonary disease.

    abstract:BACKGROUND:Impaired skeletal muscle regeneration could contribute to the progression of muscle atrophy in patients with chronic obstructive pulmonary disease (COPD). METHODS:Satellite cells and myogenesis-related proteins were compared between healthy subjects and patients with COPD, with or without muscle atrophy. Sa...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Thériault ME,Paré MÈ,Lemire BB,Maltais F,Debigaré R

    更新日期:2014-03-25 00:00:00

  • Chitinase-like protein YKL-40 correlates with inflammatory phenotypes, anti-asthma responsiveness and future exacerbations.

    abstract:BACKGROUND:Asthma is a heterogeneous chronic airway disease, which may be classified into different phenotypes. YKL-40 is a chitin-binding glycoprotein with unclear functions, but its expression is associated with inflammation and tissue remodeling. However, few studies have explored whether YKL-40 is associated with i...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Liu L,Zhang X,Liu Y,Zhang L,Zheng J,Wang J,Hansbro PM,Wang L,Wang G,Hsu AC

    更新日期:2019-05-22 00:00:00

  • The clinical course of idiopathic pulmonary fibrosis and its association to quality of life over time: longitudinal data from the INSIGHTS-IPF registry.

    abstract:BACKGROUND:Quality of life (QoL) is profoundly impaired in patients with idiopathic pulmonary fibrosis (IPF). However, data is limited regarding the course of QoL. We therefore analysed longitudinal data from the German INSIGHTS-IPF registry. METHODS:Clinical status and QoL were assessed at enrollment and subsequently...

    journal_title:Respiratory research

    pub_type: 杂志文章,多中心研究


    authors: Kreuter M,Swigris J,Pittrow D,Geier S,Klotsche J,Prasse A,Wirtz H,Koschel D,Andreas S,Claussen M,Grohé C,Wilkens H,Hagmeyer L,Skowasch D,Meyer JF,Kirschner J,Gläser S,Kahn N,Welte T,Neurohr C,Schwaiblmair M,Held

    更新日期:2019-03-15 00:00:00

  • Impact of interleukin-6 on hypoxia-induced pulmonary hypertension and lung inflammation in mice.

    abstract:BACKGROUND:Inflammation may contribute to the pathogenesis of various forms of pulmonary hypertension (PH). Recent studies in patients with idiopathic PH or PH associated with underlying diseases suggest a role for interleukin-6 (IL-6). METHODS:To determine whether endogenous IL-6 contributes to mediate hypoxic PH and...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Savale L,Tu L,Rideau D,Izziki M,Maitre B,Adnot S,Eddahibi S

    更新日期:2009-01-27 00:00:00

  • Exchange protein directly activated by cAMP (Epac) protects against airway inflammation and airway remodeling in asthmatic mice.

    abstract:BACKGROUND:β2 receptor agonists induce airway smooth muscle relaxation by increasing intracellular cAMP production. PKA is the traditional downstream signaling pathway of cAMP. Exchange protein directly activated by cAMP (Epac) was identified as another important signaling molecule of cAMP recently. The role of Epac in...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Chen YF,Huang G,Wang YM,Cheng M,Zhu FF,Zhong JN,Gao YD

    更新日期:2019-12-18 00:00:00

  • Altered molecular specificity of surfactant phosphatidycholine synthesis in patients with acute respiratory distress syndrome.

    abstract:BACKGROUND:Acute respiratory distress syndrome (ARDS) is a life-threatening critical illness, characterised by qualitative and quantitative surfactant compositional changes associated with premature airway collapse, gas-exchange abnormalities and acute hypoxic respiratory failure. The underlying mechanisms for this dys...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Dushianthan A,Goss V,Cusack R,Grocott MP,Postle AD

    更新日期:2014-11-07 00:00:00

  • Budesonide/formoterol and formoterol provide similar rapid relief in patients with acute asthma showing refractoriness to salbutamol.

    abstract:BACKGROUND:To compare the efficacy and safety of budesonide/formoterol (Symbicort) with formoterol (Oxis) in the treatment of patients with acute asthma who showed evidence of refractoriness to short-acting beta2-agonist therapy. METHODS:In a 3 hour, randomized, double-blind study, a total of 115 patients with acute a...

    journal_title:Respiratory research

    pub_type: 杂志文章,随机对照试验


    authors: Bateman ED,Fairall L,Lombardi DM,English R

    更新日期:2006-01-24 00:00:00

  • Thank you and farewell after 15 years editing respiratory research.

    abstract:: ...

    journal_title:Respiratory research

    pub_type: 社论


    authors: Lotvall J,Panettieri RA Jr

    更新日期:2018-11-26 00:00:00

  • Identification of vitamin D sensitive pathways during lung development.

    abstract:BACKGROUND:We have previously shown that vitamin D deficiency has a detrimental impact on lung development. In this study, we aimed to identify the mechanisms linking vitamin D with lung development using a mouse model of dietary manipulation. METHODS:Female offspring were euthanized at different time-points; embryoni...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Chen L,Wilson R,Bennett E,Zosky GR

    更新日期:2016-04-27 00:00:00

  • Airway inflammation and mannitol challenge test in COPD.

    abstract:BACKGROUND:Eosinophilic airway inflammation has successfully been used to tailor anti-inflammatory therapy in chronic obstructive pulmonary disease (COPD). Airway hyperresponsiveness (AHR) by indirect challenges is associated with airway inflammation. We hypothesized that AHR to inhaled mannitol captures eosinophilia i...

    journal_title:Respiratory research

    pub_type: 杂志文章,随机对照试验


    authors: de Nijs SB,Fens N,Lutter R,Dijkers E,Krouwels FH,Smids-Dierdorp BS,van Steenwijk RP,Sterk PJ

    更新日期:2011-01-18 00:00:00

  • Endotoxin receptor CD14 in PiZ alpha-1-antitrypsin deficiency individuals.

    abstract:BACKGROUND:CD14, a receptor for lipopolysaccharides (LPS), is found in both a membrane-bound form (mCD14) and a soluble form (sCD14). It is suggested that sCD14 is mainly released from blood monocytes by serine protease-mediated shedding. Because alpha1-antitrypsin (AAT), an inhibitor of serine proteases, has been show...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Sandström CS,Novoradovskaya N,Cilio CM,Piitulainen E,Sveger T,Janciauskiene S

    更新日期:2008-04-21 00:00:00

  • Decline of COPD exacerbations in clinical trials over two decades - a systematic review and meta-regression.

    abstract:BACKGROUND:An important goal of chronic obstructive pulmonary disease (COPD) treatment is to reduce the frequency of exacerbations. Some observations suggest a decline in exacerbation rates in clinical trials over time. A more systematic understanding would help to improve the design and interpretation of COPD trials. ...

    journal_title:Respiratory research

    pub_type: 杂志文章,meta分析


    authors: Andreas S,Röver C,Heinz J,Straube S,Watz H,Friede T

    更新日期:2019-08-16 00:00:00

  • Characteristics, disease burden and costs of COPD patients in the two years following initiation of long-acting bronchodilators in UK primary care.

    abstract:BACKGROUND:To assess the symptomatic and cost burden among patients initiating long-acting bronchodilator (LABD) therapy and impact of adherence on healthcare resource use and costs. METHODS:This retrospective cohort study identified patients with COPD who were newly prescribed a LABD (long-acting muscarinic antagonis...

    journal_title:Respiratory research

    pub_type: 杂志文章


    authors: Punekar YS,Landis SH,Wurst K,Le H

    更新日期:2015-11-16 00:00:00