Abstract:
BACKGROUND:Epithelial-mesenchymal transition (EMT) has been believed to be related with chemotherapy resistance in non-small cell lung cancer (NSCLC). Recent studies have suggested eIF5A-2 may function as a proliferation-related oncogene in tumorigenic processes. METHODS:We used cell viability assays, western blotting, immunofluorescence, transwell-matrigel invasion assay, wound-healing assay combined with GC7 (a novel eIF5A-2 inhibitor) treatment or siRNA interference to investigate the role of eIF5A-2 playing in NSCLC chemotherapy. RESULTS:We found low concentrations of GC7 have little effect on NSCLC viability, but could enhance cisplatin cytotoxicity in NSCLC cells. GC7 also could reverse mesenchymal phenotype in NCI-H1299 and prevented A549 cells undergoing EMT after TGF-β1 inducement. eIF5A-2 knockdown resulted in EMT inhibition. CONCLUSION:Our data indicated GC7 enhances cisplatin cytotoxicity and prevents the EMT in NSCLC cells by inhibiting eIF5A-2.
journal_name
BMC Pulm Medjournal_title
BMC pulmonary medicineauthors
Xu G,Yu H,Shi X,Sun L,Zhou Q,Zheng D,Shi H,Li N,Zhang X,Shao Gdoi
10.1186/1471-2466-14-174subject
Has Abstractpub_date
2014-11-07 00:00:00pages
174issn
1471-2466pii
1471-2466-14-174journal_volume
14pub_type
杂志文章abstract:BACKGROUND:Pulmonary surfactant D (SP-D) has important regulatory functions for innate immunity and has been implicated as a biomarker for chronic obstructive pulmonary disease (COPD). We hypothesized that COPD patients would have reduced bronchoalveolar lavage (BAL) fluid SP-D levels compared to healthy smoking and no...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/1471-2466-10-53
更新日期:2010-10-25 00:00:00
abstract:BACKGROUND:COVID-19 is a systemic viral infection which mainly targets the human respiratory system with many secondary clinical manifestations especially affecting the hematopoietic system and haemostasis. Few studies have highlighted the prognostic value of blood findings such as lymphopenia, neutrophil/lymphocyte ra...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-020-01389-z
更新日期:2021-01-22 00:00:00
abstract:BACKGROUND:Our study aimed to verify the prognostic value of circulating tumor cells (CTCs) prior to initial treatment on survival of non-small cell lung cancer (NSCLC) by using meta-analysis and system review of published studies. MATERIALS AND METHODS:The PubMed, EMBASE and Cochrane Library were searched, respective...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章,meta分析
doi:10.1186/s12890-019-1029-x
更新日期:2019-12-26 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12890-020-1176-0
更新日期:2020-05-20 00:00:00
abstract:BACKGROUND:Despite the recent increasing worldwide attention towards pulmonary hypertension (PH), its epidemiology remains poorly described in Africa. Accordingly, we performed a systematic review and meta-analysis of PH prevalence, incidence and etiologies in Africa. METHODS:We searched PubMed, EMBASE, African Journa...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s12890-017-0549-5
更新日期:2017-12-08 00:00:00
abstract:BACKGROUND:The assessment of airway inflammation for the diagnosis of asthma or COPD is still uncommon in pneumology-specialized general practices. In this respect, the measurement of exhaled nitric oxide (NO), as a fast and simple methodology, is increasingly used. The indirect assessment of airway inflammation, howev...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/1471-2466-8-5
更新日期:2008-02-28 00:00:00
abstract:BACKGROUND:Idiopathic Pulmonary Fibrosis (IPF) is a fatal fibrotic lung disease occurring predominantly in middle-aged and older adults. The traditional diagnostic classification of IPF is based on clinical, radiological, and histopathological features. However, the considerable heterogeneity in IPF presentation sugges...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-017-0472-9
更新日期:2017-10-20 00:00:00
abstract:BACKGROUND:Clinical guidelines specify that diagnosis of interstitial pulmonary fibrosis (IPF) requires identification of usual interstitial pneumonia (UIP) pattern. While UIP can be identified by high resolution CT of the chest, the results are often inconclusive, making surgical lung biopsy necessary to reach a defin...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-017-0485-4
更新日期:2017-11-17 00:00:00
abstract:BACKGROUND:It has been recognized that despite previous stability some patients with idiopathic pulmonary fibrosis (IPF) experience acute clinical deteriorations called acute exacerbations of idiopathic pulmonary fibrosis (AEX-IPF). We hypothesized that pulmonary infection can be excluded based on clinical and laborato...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-015-0066-3
更新日期:2015-07-10 00:00:00
abstract:BACKGROUND:The use of extracorporeal membrane oxygenation (ECMO) in awake, spontaneously breathing and non-intubated patients (awake ECMO) may be a novel therapeutic strategy for severe acute respiratory distress syndrome (ARDS) patients. The purpose of this study is to assess the feasibility and safety of awake ECMO i...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-019-1016-2
更新日期:2019-12-09 00:00:00
abstract:BACKGROUND:Neurohumoral activation is present in COPD and might provide a link between pulmonary and systemic effects, especially cardiovascular disease. Because long acting inhaled β-agonists reduce hyperinflation, they could reduce sympathoexcitation by improving the inflation reflex. We aimed to evaluate if inhaled ...
journal_title:BMC pulmonary medicine
pub_type: 临床试验,杂志文章
doi:10.1186/s12890-015-0054-7
更新日期:2015-04-30 00:00:00
abstract:BACKGROUND:To investigate if age, gender and smoking are associated with airway wall thickness (AWT) measured by high resolution computed tomography (HRCT) and if higher AWT is associated with lower levels of pulmonary function in healthy current- and never-smokers with a wide age range. METHODS:HRCT scans were perfor...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-017-0363-0
更新日期:2017-02-01 00:00:00
abstract:BACKGROUND:Asthma is a common chronic disease among young adults, and several studies have reported increased mortality rates in patients with asthma. However, no study has described sudden unexpected death in a nationwide setting in patients with uncontrolled asthma. We defined uncontrolled asthma as a previous hospit...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-015-0033-z
更新日期:2015-04-14 00:00:00
abstract:BACKGROUND:To investigate the use of do-not-resuscitate (DNR) orders in patients hospitalized with community-acquired pneumonia (CAP) and the association with mortality. METHODS:We assembled a cohort of 1317 adults hospitalized with radiographically confirmed CAP in three Danish hospitals. Patients were grouped into n...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-020-01236-1
更新日期:2020-07-24 00:00:00
abstract:BACKGROUND:Telehealth and telemonitoring is an emerging area of study in people with cystic fibrosis (CF), with the potential of increasing access to care, and minimising infection control risks to patients without compromising their health outcomes. To date, limited evidence is available to support the use of teleheal...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-019-0784-z
更新日期:2019-01-24 00:00:00
abstract:BACKGROUND:The pathological appearance of idiopathic pleuroparenchymal fibroelastosis (IPPFE) with hematoxylin-eosin staining is similar to that of usual interstitial pneumonia (UIP) in patients with idiopathic pulmonary fibrosis (IPF). The amount of elastic fibers (EF) and detailed differences between IPPFE and IPF ha...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/1471-2466-14-91
更新日期:2014-05-28 00:00:00
abstract:BACKGROUND:Gene expression changes in the structural cells of the airways are thought to play a role in the development of asthma and airway hyperresponsiveness. This includes changes to smooth muscle contractile machinery and epithelial barrier integrity genes. We used a targeted gene expression arrays to identify cha...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-017-0545-9
更新日期:2017-12-11 00:00:00
abstract:BACKGROUND:Technology offers opportunities to improve healthcare, but little is known about Internet use by COPD patients. We tested two hypotheses: Internet access is associated with socio-demographic disparities and frequency of use is related to perceived needs. METHODS:We analyzed data from a 2007-2008 national co...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/1471-2466-14-66
更新日期:2014-04-22 00:00:00
abstract:BACKGROUND:The 2013 GOLD classification system for COPD distinguishes four stages: A (low symptoms, low exacerbation risk), B (high symptoms, low risk), C (low symptoms, high risk) and D (high symptoms, high risk). Assessment of risk is based on exacerbation history and airflow obstruction, whatever results in a higher...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2466-14-163
更新日期:2014-10-18 00:00:00
abstract:BACKGROUND:During the pathological destruction of lung tissue, neutrophil elastase (NE) degrades elastin, one of the major constituents of lung parenchyma. However there are no non-invasive methods to quantify NE degradation of elastin. We selected specific elastin fragments generated by NE for antibody generation and ...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-015-0048-5
更新日期:2015-05-03 00:00:00
abstract:BACKGROUND:Noninvasive ventilation (NIV) is used in critically ill patients with acute respiratory failure (ARF) to avoid endotracheal intubation. However, the impact of NIV use on ARF patient's outcomes is still unclear. Our objectives were to evaluate the rate of NIV failure in hypoxemic patients with an arterial car...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-015-0139-3
更新日期:2015-11-11 00:00:00
abstract:BACKGROUND:Fine needle aspiration is an important tool for diagnosis and preoperative evaluation of solitary nodules of the lung. It provides a definitive diagnosis in most patients at low cost with minimal trauma. However, because of the nature of the study and the presentation of the cells in a more distorted and inc...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/1471-2466-3-2
更新日期:2003-09-08 00:00:00
abstract:BACKGROUND:Liver dysfunction reflects the status of heart failure, with congestion and low perfusion of the liver serving as causative mechanisms. Previous studies demonstrated relationship between the results of liver function test and the prognosis in patients with heart failure. However, few studies have examined th...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/1471-2466-10-22
更新日期:2010-04-22 00:00:00
abstract:BACKGROUND:Aspergillus fumigatus is frequently encountered in sputum samples of patients with cystic fibrosis (CF), which traditionally has been interpreted as saprophytic airway colonization. However, this mere bystander role has been challenged by recent data. There is now evidence that Aspergillus fumigatus accelera...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-018-0614-8
更新日期:2018-03-27 00:00:00
abstract:BACKGROUND:Community-acquired pneumonia (CAP) requires urgent and specific antimicrobial therapy. However, the causal pathogen is typically unknown at the point when anti-infective therapeutics must be initiated. Physicians synthesize information from diverse data streams to make appropriate decisions. Artificial intel...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-020-1089-y
更新日期:2020-03-06 00:00:00
abstract:BACKGROUND:Combined effects of leaks, mechanical property of respiratory system and upper airway (UA) patency on patient-ventilator synchrony (PVA) and the level of clinically "tolerable" leaks are not well established in home ventilators. METHODS:We comparatively assessed on a bench model, the highest leak level tole...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-017-0487-2
更新日期:2017-11-21 00:00:00
abstract:BACKGROUND:Convenient approaches for accurate biopsy are extremely important to the diagnosis of lung cancer. We aimed to systematically review the clinical updates and development trends of approaches for biopsy, i.e., CT-guided PTNB (Percutaneous Transthoracic Needle Biopsy), ENB (Electromagnetic Navigation Bronchosc...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-018-0713-6
更新日期:2018-09-03 00:00:00
abstract:BACKGROUND:Treatment of patients with Cystic Fibrosis homozygous for the Phe508del gene, with Lumacaftor /Ivacaftor (LUM/IVA) improves outcomes in patients with FEV1 > 40% predicted. We set out to observe the most sensitive clinical measure that would change with treatment in terms of exercise capacity or lung function...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-019-0866-y
更新日期:2019-06-17 00:00:00
abstract:BACKGROUND:The six-minute walk test (6MWT) is a simple, low tech, safe and well established, self-paced assessment tool to quantify functional exercise capacity in adults. The definition of normal 6MWT in children is especially demanding since not only parameters like height, weight and ethnical background influence th...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/1471-2466-13-49
更新日期:2013-08-05 00:00:00
abstract:BACKGROUND:Pulmonary alveolar proteinosis (PAP) is a rare lung disease characterized by accumulation of phospholipoproteinaceous material in the alveoli. The presentation is nonspecific but typically includes dyspnea; the spectrum of disease includes rapidly progressive hypoxic respiratory failure. Whole lung lavage (W...
journal_title:BMC pulmonary medicine
pub_type: 杂志文章
doi:10.1186/s12890-019-0822-x
更新日期:2019-03-12 00:00:00