Effects of KH-204 on the expression of heat shock protein 70 and germ cell apoptosis in infertility rat models.

Abstract:

BACKGROUND:Idiopathic infertility is a significant number of causes of male infertility. Empirical treatments are used for idiopathic male infertility, and antioxidant supplementation is a kind of management of oxidative stress related infertility. We investigated the antioxidant effects of the modified Ojayeonjonghwan (KH-204) in a rat model of cryptorchidism. METHOD:Male rats were divided into four groups (n = 8 in each): a normal control group, a cryptorchidism-induced control group and two cryptorchidism-induced groups treated p.o. with either 200 or 400 mg/kg, KH-204 for 4 weeks. The testes and epididymides from rats in all groups were removed, weighed and subjected to histological examination and semen analysis after surgery. Oxidative stress was assessed by measuring 8-hydroxy-20-deoxyguanosine (8-OHdG), superoxide dismutase (SOD) and heat shock protein (HSP) levels. Apoptosis was determined using a terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling assay. RESULTS:Treatment with the multi-herbal medicine KH-204 (1) increased the mean weight of the cryptorchid testes; (2) restored sperm counts, motility and germinal cell layer thickness; (3) decreased levels of 8-OHdG and increased levels of SOD; and (4) decreased HSP70 levels and apoptosis. CONCLUSIONS:KH-204 reduces the oxidative stress in an experimental rat model of cryptorchidism, and it may alleviate HSP expression and germ cell apoptosis.

authors

Bae WJ,Ha US,Kim KS,Kim SJ,Cho HJ,Hong SH,Lee JY,Wang Z,Hwang SY,Kim SW

doi

10.1186/1472-6882-14-367

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

367

issn

1472-6882

pii

1472-6882-14-367

journal_volume

14

pub_type

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