Abstract:
BACKGROUND:Grape skin, a rich source of phytochemicals, has been reported to possess remarkable anti-obesity activity. Fatty acid synthase (FAS) is a key enzyme catalyzing the synthesis of fatty acid de novo, and has been considered as an anti-obesity target. To elucidate the anti-obesity mechanism of grape skin, we investigated the effects of grape skin extract (GSE) and resveratrol, one of the phytochemicals in GSE, on FAS and FAS over-expressed 3 T3-L1 preadipocyte. METHODS:Purified FAS was obtained from chicken liver. Dried grape skin was extracted by 50% ethanol and partitioned by ethyl acetate. Inhibitory effects of GSE and resveratrol on FAS including fast-binding inhibition, time-dependent inhibition, and enzyme kinetics were determined. Inhibitory effects of GSE and resveratrol on 3T3-L1 preadipocyte were also measured. RESULTS:GSE inhibited the overall reaction and β-ketoacyl reductase (KR) reaction of FAS with IC50 values of 4.61 μg/ml and 20.3 μg/ml. For inhibition by resveratrol, the relevant IC50 values were 11.1 μg/ml and 21.9 μg/ml, respectively. And both GSE and resveratrol showed time-dependent inhibition for FAS, with the kobs values of 0.028 min-1, and 0.040 min-1 respectively. They inhibited the overall reaction of FAS competitively with acetyl-CoA, noncompetitively with malonyl-CoA and in a mixed manner with NADPH. Moreover, the inhibition on KR domain by resveratrol was time-dependent with kobs value of 0.106 min-1. In 3 T3-L1 preadipocytes, resveratrol reduced lipid accumulation remarkably. CONCLUSIONS:GSE and resveratrol are potent FAS inhibitors and they bound reversibly to the KR domain of FAS to inhibit the reduction of the saturated acyl groups in fatty acid synthesis. Based on the valid data and deliberate analysis, we proposed that GSE and resveratrol have great medical potential and officinal value in treating obesity and related diseases.
journal_name
BMC Complement Altern Medjournal_title
BMC complementary and alternative medicineauthors
Liang Y,Tian W,Ma Xdoi
10.1186/1472-6882-13-361subject
Has Abstractpub_date
2013-12-16 00:00:00pages
361issn
1472-6882pii
1472-6882-13-361journal_volume
13pub_type
杂志文章abstract:BACKGROUND:Postmenopausal women experience estrogen deficiency-related menopausal symptoms (e.g., hot flashes and mood swings) and a dramatic increase in the incidence of chronic diseases. Although estrogen-replacement therapy (ERT) can reduce mortality from cardiovascular disease and improve osteoporosis and menopausa...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-12-260
更新日期:2012-12-21 00:00:00
abstract:BACKGROUND:JC-001 is a Chinese medicine that can modulate the immunity in Hepa 1-6 tumor-bearing mice, and we questioned whether JC-001 can serve as efficient adjuvant chemotherapy. We aimed to identify a novel approach for enhancing cis-diamminedichloroplatinum (II) (CDDP)-based chemotherapy by immunomodulation. METH...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-213
更新日期:2013-08-23 00:00:00
abstract:CORRECTION:After the publication of this article [1] it came to our attention that Harquin Simplice Foyet was incorrectly included as Harquin Simplice Harquin Foyet. The corrected name is included in the author list. The original article was updated. ...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12906-017-2069-5
更新日期:2017-12-28 00:00:00
abstract:BACKGROUND:Storage lower urinary tract symptoms (LUTS) including overactive bladder (OAB) and urinary incontinence (UI) affect millions of people worldwide, significantly impacting quality of life. Plant based medicines have been documented both empirically and in emerging scientific research to have varying benefits i...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12906-018-2101-4
更新日期:2018-01-31 00:00:00
abstract:BACKGROUND:Ginsenoside Rg1 (Rg1) is one of the most active ingredients in Panax ginseng and has been proven to have anti-oxidative and anti-aging properties. However, there have been few reports concerning the anti-aging effects of Rg1 on the hematopoietic microenvironment and bone marrow stromal cells (BMSCs). METHOD...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-015-0642-3
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doi:10.1186/1472-6882-4-18
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-10-24
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,meta分析
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,评审
doi:10.1186/s12906-015-0663-y
更新日期:2015-05-07 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
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更新日期:2015-07-16 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-14-112
更新日期:2014-03-28 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-13-312
更新日期:2013-11-11 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-017-2040-5
更新日期:2017-12-13 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-018-2303-9
更新日期:2018-10-05 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-14-20
更新日期:2014-01-13 00:00:00
abstract::Following publication of the original article [1], the author reported that Tables 3 and 4 were incorrect due to a production error. ...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12906-019-2658-6
更新日期:2019-11-07 00:00:00
abstract:BACKGROUND:Consumption of plant-derived nutraceuticals and crude drugs in traditional medicine is widely believed to confer beneficial effects in thwarting the progression of cardiovascular diseases. Rhus tripartita (family Anacardiaceae) has been traditionally used to treat a wide range of ailments. METHODS:In the pr...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
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journal_title:BMC complementary and alternative medicine
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journal_title:BMC complementary and alternative medicine
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pub_type: 杂志文章
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更新日期:2016-09-06 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
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更新日期:2015-06-30 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
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更新日期:2016-07-02 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
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更新日期:2015-03-03 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-016-1285-8
更新日期:2016-08-24 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-015-0602-y
更新日期:2015-03-21 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-016-1328-1
更新日期:2016-09-08 00:00:00
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journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-016-1545-7
更新日期:2017-01-07 00:00:00
abstract:BACKGROUND:Isoflavones are naturally occurring plant chemicals belonging to the "phytoestrogen" class. The aim of the present study was to examine the effects of isoflavones obtained from Cordyceps sinensis (CSIF) on development of estrogen deficiency-induced osteoporosis in ovariectomized rats. METHODS:After the rats...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/1472-6882-14-484
更新日期:2014-12-13 00:00:00
abstract:BACKGROUND:In contrast to the guidelines of World Health Organization (WHO) and United States-Centers for Disease Control and prevention (US-CDC), the Chinese national guidelines recommend the use of steroids, intravenous immunoglobulin (IVIG), or traditional Chinese herbs (TCHs) in hand-foot-mouth disease (HFMD) manag...
journal_title:BMC complementary and alternative medicine
pub_type: 杂志文章
doi:10.1186/s12906-018-2259-9
更新日期:2018-06-20 00:00:00