Topical application of Acheflan on rat skin injury accelerates wound healing: a histopathological, immunohistochemical and biochemical study.

Abstract:

BACKGROUND:Dermal wound healing involves a cascade of complex events including angiogenesis and extracellular matrix remodeling. Several groups have focused in the study of the skin wound healing activity of natural products. The phytomedicine Acheflan®, and its main active constituent is the oil from Cordia verbenacea which has known anti-inflammatory, analgesic and antimicrobial activities. To our knowledge, no investigation has evaluated the effect of Acheflan® in an experimental model of skin wound healing. The present study has explored the wound healing property of Acheflan® and has compared it with topical effectiveness of collagenase and fibrinolysin by using Wistar rat cutaneous excision wound model. METHODS:Animals were divided into four groups: untreated animals are negative control (NC), wounds were treated topically every day with Collagenase ointment (TC), with Fibrinolysin ointment (TF) and with cream Acheflan (TAc). Skin samples were collected on zero, 8th and 15th days after wounding. The healing was assessed by hematoxylin-eosin (HE), picrosirius red, hydoxyproline content and immunohistochemical analysis of the vascular endothelial growth factor (VEGF) and matrix metalloprotease-9 (MMP-9). Statistical analysis was done by ANOVA and Student t-test (p < 0.05). RESULTS:The histological analysis HE of wound in the TAc group was more efficient because it was possible to observe the complete remodeling of the epidermis indicating the regression of lesions compared with the NC. The evaluation of picrosirius staining has demonstrated a significant increase of collagen distribution in the TC and TAc treatments compared with NC and TF groups. These results are corroborated with hydroxyproline content. Skin TC and TAc treated rats have showed an increase of VEGF and MMP-9 compared with NC and TF groups. All parameters were significant (P < 0.05). CONCLUSION:The phytomedicine Acheflan® (oil of Cordia verbenacea) and TC possess higher therapeutic properties for wound healing compared with TF. These ointments seem to accelerate wound healing, probably due to their involvement with the increase of angiogenesis and dermal remodeling.

authors

Perini JA,Angeli-Gamba T,Alessandra-Perini J,Ferreira LC,Nasciutti LE,Machado DE

doi

10.1186/s12906-015-0745-x

subject

Has Abstract

pub_date

2015-06-30 00:00:00

pages

203

issn

1472-6882

pii

10.1186/s12906-015-0745-x

journal_volume

15

pub_type

杂志文章
  • Molecular insights into the anti-cancer properties of traditional Tibetan medicine Yukyung Karne.

    abstract:BACKGROUND:Yukyung karne (YK) is a traditional Tibetan formulation used for many centuries for the treatment of ovarian cancer. However, the pharmacological basis of its anticancer property is not well understood. In the present study, the anticancer property of YK was investigated in cell culture. METHODS:The growth ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-14-380

    authors: Choedon T,Dolma D,Mathan G,Kumar V

    更新日期:2014-10-07 00:00:00

  • Antioxidant, anti-inflammatory and cytotoxicity of Phaleria macrocarpa (Boerl.) Scheff Fruit.

    abstract:BACKGROUND:Phaleria macrocarpa (Scheff.) Boerl (Thymelaceae) originates from Papua Island, Indonesia and grows in tropical areas. The different parts of the fruit of P. macrocarpa were evaluated for antioxidant, anti-inflammatory, and cytotoxic activities. METHODS:Phaleria macrocarpa fruit were divided into pericarp, ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-11-110

    authors: Hendra R,Ahmad S,Oskoueian E,Sukari A,Shukor MY

    更新日期:2011-11-09 00:00:00

  • Changes in antioxidant and antibacterial activities as well as phytochemical constituents associated with ginger storage and polyphenol oxidase activity.

    abstract:BACKGROUND:Herbal materials should be stored at optimal conditions in order to retain their nutritional quality. Proper storage has a significant impact on the quality of the herbs and spices. METHODS:The effects of storage temperature (5 and 15 °C) and time (4 and 8 months) on the phytochemical constituents associate...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1352-1

    authors: Ghasemzadeh A,Jaafar HZ,Rahmat A

    更新日期:2016-09-29 00:00:00

  • Hypotensive effect of Gentiana floribunda is mediated through Ca++ antagonism pathway.

    abstract:BACKGROUND:Gentiana floribunda was investigated for the possible hypotensive and vasodilator activities in an attempt to rationalize its traditional use in hypertension. METHODS:The crude extract of Gentiana floribunda (Gf.Cr) was studied in anaesthetized rats and isolated thoracic aorta tissues. RESULTS:Gf.Cr which ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-12-121

    authors: Khan AU,Mustafa MR,Khan AU,Murugan DD

    更新日期:2012-08-11 00:00:00

  • Correction to: Effects of transcutaneous electrical nerve stimulation in the Management of PostInjection Sciatic Pain in a non-randomized controlled clinical trial in Nnewi, Nigeria.

    abstract::ᅟ. ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章,已发布勘误

    doi:10.1186/s12906-018-2398-z

    authors: Okonkwo UP,Ibeneme SC,Ihegihu EY,Egwuonwu AV,Ezema IC,Maruf AF,Okoye EC,Ibikunle OP,Ezeukwu AO

    更新日期:2019-01-08 00:00:00

  • Preclinical evaluation of safety and potential of black hellebore extracts for cancer treatment.

    abstract:BACKGROUND:The therapeutic use of Helleborus niger L. is manifold due to its specific phytochemical composition. Two compound groups, the ranunculin derivates including protoanemonin and the steroidal saponins, are also associated with toxicity (genotoxicity, disintegration of membrane structures). Therefore, in vitro ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-019-2517-5

    authors: Felenda JE,Turek C,Mörbt N,Herrick A,Müller MB,Stintzing FC

    更新日期:2019-05-21 00:00:00

  • Optimization of extraction parameters of PTP1β (protein tyrosine phosphatase 1β), inhibitory polyphenols, and anthocyanins from Zea mays L. using response surface methodology (RSM).

    abstract:BACKGROUND:Protein tyrosine phosphatase expressed in insulin-sensitive tissues (such as liver, muscle, and adipose tissue) has a key role in the regulation of insulin signaling and pathway activation, making protein tyrosine phosphatase a promising target for the treatment of type 2 diabetes mellitus and obesity and re...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1296-5

    authors: Hwang SH,Kwon SH,Wang Z,Kim TH,Kang YH,Lee JY,Lim SS

    更新日期:2016-08-26 00:00:00

  • Protective effects of Oxya chinensis sinuosa Mishchenko against ultraviolet B-induced photodamage in hairless mice.

    abstract:BACKGROUND:Edible insects, including Oxya chinensis sinuosa Mishchenko (Oc), which is consumed as food in Asia, are considered as a human food shortage alternative, and also as a preventive measure against environmental destruction. Ultraviolet B (UVB) irradiation, which causes skin photodamage, is considered as an ext...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-019-2692-4

    authors: Im AR,Park I,Ji KY,Lee JY,Kim KM,Na M,Chae S

    更新日期:2019-10-28 00:00:00

  • Physician and patient attitudes towards complementary and alternative medicine in obstetrics and gynecology.

    abstract:BACKGROUND:In the U.S., complementary and alternative medicine (CAM) use is most prevalent among reproductive age, educated women. We sought to determine general attitudes and approaches to CAM among obstetric and gynecology patients and physicians. METHODS:Obstetrician-gynecologist members of the American Medical Ass...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-8-35

    authors: Furlow ML,Patel DA,Sen A,Liu JR

    更新日期:2008-06-26 00:00:00

  • Antioxidant potential, in vitro cytotoxicity and apoptotic effect induced by crude organic extract of Anthracophyllum lateritium against RD sarcoma cells.

    abstract:BACKGROUND:Macrofungi have an established history of use in traditional oriental medicine. Anthracophyllum lateritium is a terrestrial macrofungus found in the dry zone forest reserves in Sri Lanka. Yet there are no scientific reports on bioactive properties of this species. Hence, the current study was aimed at determ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0924-9

    authors: Fernando DM,Wijesundera RL,Soysa P,de Silva D,Nanayakkara CM

    更新日期:2015-11-06 00:00:00

  • A new, easy-to-make pectin-honey hydrogel enhances wound healing in rats.

    abstract:BACKGROUND:Honey, alone or in combination, has been used for wound healing since ancient times and has reemerged as a topic of interest in the last decade. Pectin has recently been investigated for its use in various biomedical applications such as drug delivery, skin protection, and scaffolding for cells. The aim of t...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-017-1769-1

    authors: Giusto G,Vercelli C,Comino F,Caramello V,Tursi M,Gandini M

    更新日期:2017-05-16 00:00:00

  • Astragaloside IV protects against diabetic nephropathy via activating eNOS in streptozotocin diabetes-induced rats.

    abstract:BACKGROUND:Astragaloside IV (AS-IV) was reported to play a role in improving diabetic nephropathy (DN), however, the underlying mechanisms still remain unclear. The aim of the present study is to investigate whether AS-IV ameliorates DN via the regulation of endothelial nitric oxide synthase (eNOS). METHODS:DN model w...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-019-2728-9

    authors: Fan Y,Fan H,Zhu B,Zhou Y,Liu Q,Li P

    更新日期:2019-12-05 00:00:00

  • Antimicrobial activity against oral pathogens and immunomodulatory effects and toxicity of geopropolis produced by the stingless bee Melipona fasciculata Smith.

    abstract:BACKGROUND:Native bees of the tribe Meliponini produce a distinct kind of propolis called geopropolis. Although many pharmacological activities of propolis have already been demonstrated, little is known about geopropolis, particularly regarding its antimicrobial activity against oral pathogens. The present study aimed...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-11-108

    authors: Liberio SA,Pereira AL,Dutra RP,Reis AS,Araújo MJ,Mattar NS,Silva LA,Ribeiro MN,Nascimento FR,Guerra RN,Monteiro-Neto V

    更新日期:2011-11-04 00:00:00

  • In vitro determination of the anti-aging potential of four southern African medicinal plants.

    abstract:BACKGROUND:Aging is an inevitable process for all living organisms. During this process reactive oxygen species generation is increased which leads to the activation of hyaluronidase, collagenase and elastase, which can further contribute to skin aging. Four southern African medicinal plants; Clerodendrum glabrum, Scho...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-304

    authors: Ndlovu G,Fouche G,Tselanyane M,Cordier W,Steenkamp V

    更新日期:2013-11-05 00:00:00

  • Development and evaluation of an instrument for the critical appraisal of randomized controlled trials of natural products.

    abstract:BACKGROUND:The efficacy of natural products (NPs) is being evaluated using randomized controlled trials (RCTs) with increasing frequency, yet a search of the literature did not identify a widely accepted critical appraisal instrument developed specifically for use with NPs. The purpose of this project was to develop an...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-9-11

    authors: Jurgens T,Whelan AM,MacDonald M,Lord L

    更新日期:2009-04-23 00:00:00

  • Factors associated with herbal use among urban multiethnic primary care patients: a cross-sectional survey.

    abstract:BACKGROUND:The use of herbal supplements in the United States has become increasingly popular. The prevalence of herbal use among primary care patients varies in previous studies; the pattern of herbal use among urban racially/ethnically diverse primary care patients has not been widely studied. The primary objectives ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-4-18

    authors: Kuo GM,Hawley ST,Weiss LT,Balkrishnan R,Volk RJ

    更新日期:2004-12-02 00:00:00

  • Mutagenicity of Chinese traditional medicine Semen Armeniacae amarum by two modified Ames tests.

    abstract:BACKGROUND:Semen armeniacae amarum (SAA) is a Chinese traditional medicine and has long been used to control acute lower respiratory tract infection and asthma, as a result of its expectorant and antiasthmatic activities. However, its mutagenicity in vitro and in vivo has not yet been reported. The Ames test for mutage...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-9-43

    authors: Jin J,Liu B,Zhang H,Tian X,Cai Y,Gao P

    更新日期:2009-11-15 00:00:00

  • Antimicrobial activities of endophytic fungi obtained from the arid zone invasive plant Opuntia dillenii and the isolation of equisetin, from endophytic Fusarium sp.

    abstract:BACKGROUND:Opuntia dillenii is an invasive plant well established in the harsh South-Eastern arid zone of Sri Lanka. Evidence suggests it is likely that the endophytic fungal populations of O. dillenii assist the host in overcoming biotic and abiotic stress by producing biologically active metabolites. With this in min...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0722-4

    authors: Ratnaweera PB,de Silva ED,Williams DE,Andersen RJ

    更新日期:2015-07-10 00:00:00

  • TRPV1 channel inhibition contributes to the antinociceptive effects of Croton macrostachyus extract in mice.

    abstract:BACKGROUND:Previous study showed that extracts from Croton macrostachyus (Euphorbiaceae) exhibit analgesic effects in acute pain models. The present study evaluates the antinociceptive properties of the methanol/methylene chloride extract (MECM) of the stem bark of this plant using mice models of persistent inflammator...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-015-0816-z

    authors: Nguelefack TB,Dutra RC,Paszcuk AF,de Andrade EL,Calixto JB

    更新日期:2015-08-25 00:00:00

  • Chemical composition and biological activity of Rubus idaeus shoots--a traditional herbal remedy of Eastern Europe.

    abstract:BACKGROUND:The young shoots of Rubus idaeus are traditionally used as a herbal remedy in common cold, fever and flu-like infections yet there is no research concerning this plant material. The aim of the study was to evaluate the chemical composition and biological properties of raspberry shoots from 11 cultivar variet...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-14-480

    authors: Krauze-Baranowska M,Głód D,Kula M,Majdan M,Hałasa R,Matkowski A,Kozłowska W,Kawiak A

    更新日期:2014-12-12 00:00:00

  • Medicinal and ethnoveterinary remedies of hunters in Trinidad.

    abstract:BACKGROUND:Ethnomedicines are used by hunters for themselves and their hunting dogs in Trinidad. Plants are used for snakebites, scorpion stings, for injuries and mange of dogs and to facilitate hunting success. RESULTS:Plants used include Piper hispidum, Pithecelobium unguis-cati, Bauhinia excisa, Bauhinia cumanensis...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-1-10

    authors: Lans C,Harper T,Georges K,Bridgewater E

    更新日期:2001-01-01 00:00:00

  • Active chemical fractions of stem bark extract of Khaya grandifoliola C.DC and Entada africana Guill. et Perr. synergistically protect primary rat hepatocytes against paracetamol-induced damage.

    abstract:BACKGROUND:Khaya grandifoliola (Meliaceae) and Entada africana (Fabaceae) are traditionally used in Bamun (a western tribe of Cameroon) traditional medicine for the treatment of liver related diseases. In this study, the synergistic hepatoprotective effect of respective active fractions of the plants were investigated ...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1169-y

    authors: Njayou FN,Kouam AF,Simo BF,Tchana AN,Moundipa PF

    更新日期:2016-07-07 00:00:00

  • In vitro evaluation of the α-glucosidase inhibitory potential of methanolic extracts of traditionally used antidiabetic plants.

    abstract:BACKGROUND:Different plant parts of Roylea cinerea (D. Don) Baill. (Lamiaceae), Clematis grata Wall. (Ranunculaceae), Cornus capitata Wall. (Cornaceae) are traditionally used in the management of diabetes and various other diseases. METHOD:The air-dried plant parts from different plants were coarsely powdered and mace...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-019-2482-z

    authors: Bhatia A,Singh B,Arora R,Arora S

    更新日期:2019-03-25 00:00:00

  • Rhus verniciflua Stokes (RVS) and butein induce apoptosis of paclitaxel-resistant SKOV-3/PAX ovarian cancer cells through inhibition of AKT phosphorylation.

    abstract:BACKGROUND:Rhus verniciflua Stokes (RVS) belongs to the Anacardiaceae family and traditionally used for cancer treatment. RVS and butein, a major compound of RVS, were known to induce apoptosis via AKT inhibition in cancer cells. Thus, in this study, we investigated the effect of RVS and its derivative compounds (fiset...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-016-1103-3

    authors: Choi HS,Kim MK,Choi YK,Shin YC,Cho SG,Ko SG

    更新日期:2016-04-27 00:00:00

  • The role of traditional healers in the diagnosis and management of Burkitt lymphoma in Cameroon: understanding the challenges and moving forward.

    abstract:BACKGROUND:Burkittlymphoma(BL) is the most common childhood cancer in Cameroon with a reported incidence of 3 per 100,000 children under 15 years in the Northwest region. Treatment at three Baptist mission hospitals has a recorded cure rate of over 50%. Traditional medicine(TM) is recognized by the national health syst...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-017-1719-y

    authors: Afungchwi GM,Hesseling PB,Ladas EJ

    更新日期:2017-04-11 00:00:00

  • The use of complementary and alternative medicine by patients with diabetes mellitus in Bahrain: a cross-sectional study.

    abstract:BACKGROUND:CAM use is widespread, especially among patients with diabetes. The Gulf States have a high prevalence of diabetes, alongside a long tradition of CAM use. The aim of this study is to establish the prevalence of CAM use among patients with diabetes mellitus in Bahrain and to examine the characteristics of the...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-10-35

    authors: Khalaf AJ,Whitford DL

    更新日期:2010-07-14 00:00:00

  • Antinociceptive activity of petroleum ether fraction obtained from methanolic extract of Clinacanthus nutans leaves involves the activation of opioid receptors and NO-mediated/cGMP-independent pathway.

    abstract:BACKGROUND:Methanol extract (MECN) of Clinacanthus nutans Lindau leaves (family Acanthaceae) demonstrated peripherally and centrally mediated antinociceptive activity via the modulation of opioid/NO-mediated, but cGMP-independent pathway. In the present study, MECN was sequentially partitioned to obtain petroleum ether...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-019-2486-8

    authors: Zakaria ZA,Abdul Rahim MH,Mohd Sani MH,Omar MH,Ching SM,Abdul Kadir A,Ahmed QU

    更新日期:2019-04-02 00:00:00

  • Beneficial effect of compound essential oil inhalation on central fatigue.

    abstract:BACKGROUNDS:Although the physical and mental enhancement effect of essential oils have been proved, the beneficial effect of essential oil in central fatigue remains unclear. In this study, we extracted essential oils from nine aromatic plants to make a compound essential oil, and detected the therapeutic effect of cen...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-018-2375-6

    authors: Han C,Li F,Tian S,Liu Y,Xiao H,Wu X,Zhang W,Zhang W,Mao M

    更新日期:2018-11-26 00:00:00

  • Phytochemical analysis with free radical scavenging, nitric oxide inhibition and antiproliferative activity of Sarcocephalus pobeguinii extracts.

    abstract:BACKGROUND:Free radicals have been implicated in the pathogenesis of diverse metabolic disorders including cancer. Therefore, fighting against free radicals has become an important strategy in the prevention or treatment of such diseases, in addition to direct or indirect anticancer chemotherapy. Sarcocephalus pobeguin...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/s12906-017-1712-5

    authors: Mfotie Njoya E,Munvera AM,Mkounga P,Nkengfack AE,McGaw LJ

    更新日期:2017-04-04 00:00:00

  • Dietary grape polyphenol resveratrol increases mammary tumor growth and metastasis in immunocompromised mice.

    abstract:BACKGROUND:Resveratrol, a polyphenol from grapes and red wine has many health beneficial effects, including protection against cardiovascular and neurodegenerative diseases and cancer. However, our group and others have provided evidence for a dual cancer promoting or inhibitory role for resveratrol in breast cancer, d...

    journal_title:BMC complementary and alternative medicine

    pub_type: 杂志文章

    doi:10.1186/1472-6882-13-6

    authors: Castillo-Pichardo L,Cubano LA,Dharmawardhane S

    更新日期:2013-01-08 00:00:00