Abstract：
BACKGROUND:Resistance to carbapenem antibiotics is emerging worldwide among Enterobacteriaceae. To prevent hospital transmission due to unnoticed carriage of carbapenemase producing micro-organisms in newly admitted patients, or follow-up of patients in an outbreak setting, a molecular screening method was developed for detection of the most prevalent carbapenemase genes; blaOXA-48, blaVIM, blaIMP, blaNDM and blaKPC. METHODS:A real-time multiplex PCR assay was evaluated using a collection of 86 Gram negative isolates, including 62 carbapenemase producers. Seven different laboratories carried out this method and used the assay for detection of the carbapenemase genes on a selection of 20 isolates. RESULTS:Both sensitivity and specificity of the multiplex PCR assay was 100%, as established by results on the strain collection and the inter-laboratory comparisons. CONCLUSIONS:In this study, we present a multiplex real-time PCR that is a robust, reliable and rapid method for the detection of the most prevalent carbapenemases blaOXA-48, blaVIM, blaIMP, blaNDM and blaKPC, and is suitable for screening of broth cultured rectal swabs and for identification of carbapenemase genes in cultures.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
van der Zee A,Roorda L,Bosman G,Fluit AC,Hermans M,Smits PH,van der Zanden AG,Te Witt R,Bruijnesteijn van Coppenraet LE,Cohen Stuart J,Ossewaarde JMdoi
10.1186/1471-2334-14-27subject
Has Abstractpub_date
2014-01-14 00:00:00pages
27issn
1471-2334pii
1471-2334-14-27journal_volume
14pub_type
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